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Fluorescence Line Narrowing Spectrometry
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Book Synopsis Bioanalytical Applications of Fluorescence Line-Narrowing and Non-Line-Narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-Performance Liquid Chromatography by :
Download or read book Bioanalytical Applications of Fluorescence Line-Narrowing and Non-Line-Narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-Performance Liquid Chromatography written by and published by . This book was released on 2001 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are widely used analytical separation techniques with many applications in chemical, biochemical, and biomedical sciences. Conventional analyte identification in these techniques is based on retention/migration times of standards; requiring a high degree of reproducibility, availability of reliable standards, and absence of coelution. From this, several new information-rich detection methods (also known as hyphenated techniques) are being explored that would be capable of providing unambiguous on-line identification of separating analytes in CE and HPLC. As further discussed, a number of such on-line detection methods have shown considerable success, including Raman, nuclear magnetic resonance (NMR), mass spectrometry (MS), and fluorescence line-narrowing spectroscopy (FLNS). In this thesis, the feasibility and potential of combining the highly sensitive and selective laser-based detection method of FLNS with analytical separation techniques are discussed and presented. A summary of previously demonstrated FLNS detection interfaced with chromatography and electrophoresis is given, and recent results from on-line FLNS detection in CE (CE-FLNS), and the new combination of HPLC-FLNS, are shown.
Book Synopsis Fluorescence Line Narrowing Spectrometry by : Michael Joseph McGlade
Download or read book Fluorescence Line Narrowing Spectrometry written by Michael Joseph McGlade and published by . This book was released on 1983 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fluorescence Line Narrowing Spectrometry of Polycyclic Aromatic Hydrocarbons in Organic Glasses by : Johnie Charles Brown
Download or read book Fluorescence Line Narrowing Spectrometry of Polycyclic Aromatic Hydrocarbons in Organic Glasses written by Johnie Charles Brown and published by . This book was released on 1982 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fluorescence Line Narrowing and Nonlinear Spectroscopy of Octaethylporphine by : Bradford Blake Price
Download or read book Fluorescence Line Narrowing and Nonlinear Spectroscopy of Octaethylporphine written by Bradford Blake Price and published by . This book was released on 1990 with total page 714 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Bioanalytical Applications of Fluorescence Line-narrowing and Non-line-narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-performance Liquid Chromatography by : Kenneth Paul Roberts
Download or read book Bioanalytical Applications of Fluorescence Line-narrowing and Non-line-narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-performance Liquid Chromatography written by Kenneth Paul Roberts and published by . This book was released on 2001 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: After cooldown the stationary analyte plugs can be interrogated indefinitely, which leads to significantly lower detection limits. Photodegradation of the analytes is also markedly reduced relative to laser excitation at room temperature. Automated translation of the cryostat/capillary relative to the spatially fixed laser excitation beam allows for sequential analysis of the separated analytes. Lower resolution but still informative analyses can be performed at 77 K. Results are presented for depurinating polycyclic aromatic hydrocarbons adducted to DNA isolated from the urine of humans exposed to coal smoke, and from the skin of mice treated with the most potent chemical carcinogen, dibenzo[a, l]pyrene. We anticipate that combining FLN and NLN detection with CE and HPLC will be useful in the many more bioanalytical applications.
Book Synopsis LINE NARROWING AND MATRIX SITE EFFECTS IN CONDENSED PHASE SPECTRA. I. FLUORESCENCE SPECTRA OF P-DFB IN CYCLOHEXANE. II. NHBS OF RESORUFIN IN VITREOUS MATRICES (PARA-DIFLUOROBENZENE, NONPHOTOCHEMICAL HOLE BURNING-SPECTROSCOPY, H.B.S.). by : ANDREW FRANCIS CHILDS
Download or read book LINE NARROWING AND MATRIX SITE EFFECTS IN CONDENSED PHASE SPECTRA. I. FLUORESCENCE SPECTRA OF P-DFB IN CYCLOHEXANE. II. NHBS OF RESORUFIN IN VITREOUS MATRICES (PARA-DIFLUOROBENZENE, NONPHOTOCHEMICAL HOLE BURNING-SPECTROSCOPY, H.B.S.). written by ANDREW FRANCIS CHILDS and published by . This book was released on 1985 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: with these dynamical relationships. Two additional matrices: 4:1 ethanol/methanol and polyacrylic acid were also investigated as hosts for the resorufin dye.
Book Synopsis Capillary Electrophoresis-fluorescence Line Narrowing System (CE-FLNS) for On-line Structural Characterization by :
Download or read book Capillary Electrophoresis-fluorescence Line Narrowing System (CE-FLNS) for On-line Structural Characterization written by and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.
Book Synopsis Laser Techniques in Luminescence Spectroscopy by : Tuan Vo-Dinh
Download or read book Laser Techniques in Luminescence Spectroscopy written by Tuan Vo-Dinh and published by ASTM International. This book was released on 1990 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz
Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2007-12-05 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
Book Synopsis Recent Advances in Thin-Layer Chromatography by : F.A.A. Dallas
Download or read book Recent Advances in Thin-Layer Chromatography written by F.A.A. Dallas and published by Springer Science & Business Media. This book was released on 2013-11-09 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: For many years TLC has suffered from the image of being a low sensitivity, low resolution, non-quantitative teehnique, suitable for chemists, but not a tool for real ehromatographers. Whilst perhaps true in the past this attitude no longer ref1eets the eapabi1ities of modern instrumenta1ized TLC in all its many forms. This volume represents the proceedings of a meeting in Brighton in 1987 whieh formed part of a eontinuing series of one and two day events on TLC organized by the Chromatographie Soeiety either alone or, like this one, in eonjunetion with other learned bodies. These meetings are designed to keep ehromatographers up to date with the latest deve10pments and help promote a more positive image of TLC. lan Wilson November 1987 v CONTENTS The Chromatographic Society xi Contemporary Thin-layer Chromatography: An introduction IoD. Wilson INSTRUMENTATION Quantitative Methods in Thin-layer Chromatography C.F. Poole, S.K. Poole and T.A. Dean 11 Fluorescence Line-narrowing Spectroscopy: A New and Highly Selective Detection Technique for Thin-layer and Liquid Chromatography J.W. Hofstraat, C. Gooijer, U.A. Th. Brinkman and N.H. Velthorst 29 Qualitative and Quantitative Image Analysis of Fluorescence from High Performance Thi. n-layer Chromatography R.M. Belchamber, S.J. Brinkworth, H. Read and J.D.M. Roberts 37 Analytical Rotation Planar Chromatography Sz. Nyiredy, K. Dallenbach-Toelke and O. Sticher 45 Analytical and Preparative Overpressured Layer Chromatography E. Mincsovics and E. Tyihak 57 Anticircular Planar Chromatography: Analytical and Preparative Aspects H. Traitler and A.
Book Synopsis Fluorescence Line Narrowing in Ytterbium Doped Inorganic Glasses by : Richard Thomas Brundage
Download or read book Fluorescence Line Narrowing in Ytterbium Doped Inorganic Glasses written by Richard Thomas Brundage and published by . This book was released on 1985 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fluorescence Spectroscopy by : Otto S. Wolfbeis
Download or read book Fluorescence Spectroscopy written by Otto S. Wolfbeis and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides analytical chemists and biomedical scientists with an excellent summary of progress...This is a book that can be recommended to all analytical scientists interested in fluorimetry." (Analytical Chimica Acta) "This is a useful overview and gives the nonspecialist a feeling for the advantages and limitations of the methods. Overall this book is a worthwhile read and a good source of references." (TRAC) The book is divided into chapters on new methods, new appli- cations, fluorescence immunoassays, fluorometric analysis and fluorescence spectroscopy in biomedical sciences. Specific topics are fluorescence spectroscopy using synchrotron radiation, picosecond fluorescence spectroscopy, fluorescence microscopy, fluorescence scattering by synthetic polymers, fluorescence immunoassays, fluorescence for environmental monitoring, fluorescence in flow injection analysis, hydro-geological studies, fluorescence of proteins, lipids and membranes, cell fluorescence, calcium transients.
Book Synopsis Encyclopedia of Spectroscopy and Spectrometry by :
Download or read book Encyclopedia of Spectroscopy and Spectrometry written by and published by Academic Press. This book was released on 2016-09-22 with total page 3716 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition of the Encyclopedia of Spectroscopy and Spectrometry, Three Volume Set provides authoritative and comprehensive coverage of all aspects of spectroscopy and closely related subjects that use the same fundamental principles, including mass spectrometry, imaging techniques and applications. It includes the history, theoretical background, details of instrumentation and technology, and current applications of the key areas of spectroscopy. The new edition will include over 80 new articles across the field. These will complement those from the previous edition, which have been brought up-to-date to reflect the latest trends in the field. Coverage in the third edition includes: Atomic spectroscopy Electronic spectroscopy Fundamentals in spectroscopy High-Energy spectroscopy Magnetic resonance Mass spectrometry Spatially-resolved spectroscopic analysis Vibrational, rotational and Raman spectroscopies The new edition is aimed at professional scientists seeking to familiarize themselves with particular topics quickly and easily. This major reference work continues to be clear and accessible and focus on the fundamental principles, techniques and applications of spectroscopy and spectrometry. Incorporates more than 150 color figures, 5,000 references, and 300 articles for a thorough examination of the field Highlights new research and promotes innovation in applied areas ranging from food science and forensics to biomedicine and health Presents a one-stop resource for quick access to answers and an in-depth examination of topics in the spectroscopy and spectrometry arenas
Book Synopsis Luminescence Techniques in Chemical and Biochemical Analysis by : Willy R. G. Baeyens
Download or read book Luminescence Techniques in Chemical and Biochemical Analysis written by Willy R. G. Baeyens and published by CRC Press. This book was released on 1990-10-26 with total page 686 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides information on modern luminescence techniques, beginning with a general introduction to luminescence spectroscopy. Divided into two basic sections, the first dealing with fluorescence and the latter part on chemiluminescence. Topics include immunoassays, the use of chemiluminescence in flow
Book Synopsis Shpol'skii Spectroscopy and Other Site-Selection Methods by : Johannes W. Hofstraat
Download or read book Shpol'skii Spectroscopy and Other Site-Selection Methods written by Johannes W. Hofstraat and published by John Wiley & Sons. This book was released on 2000-08-07 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent technological breakthroughs, most notably in the field of lasers as well as detection and data processing, have made it possible to apply high-resolution molecular spectroscopy to such areas as environmental science, bioanalysis, and chemical physics. This book describes recent advances and applications of high-resolution molecular spectroscopy in low temperature solid matrices.
Book Synopsis High Resolution-sensitivity Charcterization of Polycyclic Aromatic Hydrocarbon-DNA Adducts Using Fluorescence Line Narrowing Spectrometry by : Richard Scott Cooper
Download or read book High Resolution-sensitivity Charcterization of Polycyclic Aromatic Hydrocarbon-DNA Adducts Using Fluorescence Line Narrowing Spectrometry written by Richard Scott Cooper and published by . This book was released on 1987 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Application of Fluorescence Line Narrowing Spectroscopy to Forensic Fiber Examination by : Andres D. Campiglia
Download or read book Application of Fluorescence Line Narrowing Spectroscopy to Forensic Fiber Examination written by Andres D. Campiglia and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: We propose to develop nondestructive analytical methodology capable of providing highly discriminating identification of textile fibers encountered as physical evidence in criminal investigations. Trace textile fiber evidence is found at numerous crime scenes and plays an important role in linking a suspect to the respective scene. Several methods currently exist for the analysis of trace fiber evidence. Microscopy based techniques are important tools for discriminating fibers with at least one distinguishable characteristic. The main advantage of these techniques is their non-destructive nature, which preserves the physical integrity of the fibers for further court examination. When fibers cannot be discriminated by non-destructive tests such as those based on microscopic, optical and physical examination, the next reasonable step is to extract the dye from both the known and questioned fiber for further chemical analysis. Many cases exist where the comparison of fibers based on the chemical composition of dyes still leaves the forensic scientist with the difficulty of assigning a probability to the common source determination. The problem stems, in part, from the large amounts of textile produced each year in replicate fiber types and colors. This problem is made more difficult by the fact that it may not be possible to discriminate between two fibers that have been dyed with highly similar dyes. This is not an uncommon situation, as there are many hundred of commercial dyes with indistinguishable colors, and minimal structural variations are encouraged by the patent process and commercial competition. The end purpose of this project is to fulfill this gap with a highly discriminating identification tool that maintains the evidentiary value of the original sample. We focus on the total fluorescence emission of fibers, In addition to the contribution of the textile dye (or dyes) to the fluorescence spectrum of the fiber, we investigate the contribution of intrinsic fluorescence impurities -- i.e. impurities imbedded into the fibers during fabrication of garments -- as a reproducible source of fiber comparison. The methodology, instrumentation and data analysis we propose obviates the need for destructive dye extractions while significantly increasing the discriminating power of fluorescence microscopy.
