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Extracellular Matrix For Cardiovascular Reconstruction
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Book Synopsis Extracellular Matrix for Cardiovascular Reconstruction by : Steve Bibevski
Download or read book Extracellular Matrix for Cardiovascular Reconstruction written by Steve Bibevski and published by Frontiers Media SA. This book was released on 2021-06-01 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topic editor Robert G. Matheny is employed by company CorMatrix Inc.. All other topic editors declare no competing interests with regards to the Research Topic subject.
Book Synopsis Cardiac Extracellular Matrix by : Eric G. Schmuck
Download or read book Cardiac Extracellular Matrix written by Eric G. Schmuck and published by Springer. This book was released on 2018-09-20 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book on cardiac extracellular matrix (ECM) features three sections, Fundamental Science, Pre-Clinical and Translational Science, and Clinical Applications. In the Fundamental Science section, we will cover the spectrum of basic ECM science from ECM’s role in development, biomechanical properties, cardiac ECM influence of cardiomyocyte biology, pathophysiology of ECM in heart disease, and ECM in tissue engineering. Section two, Preclinical and Translational Science, will discuss cardiac ECM technologies in the clinical pipeline including approaches to ECM as a therapeutic, animal models of cardiac research, tracking and imaging methods of cardiac ECM, and cGMP manufacturing and regulatory considerations for ECM based therapeutics. Finally, the third section, Clinical Applications, will highlight the clinical experience around cardiac ECM including therapeutic strategies targeting scar tissue in the heart, Clinical trial design and regulatory considerations, current human clinical trials in cardiovascular medicine and the role of pharmaceutical and biotech companies in the commercialization of ECM technologies for cardiovascular indications. This book provides a comprehensive review for basic and translational researchers as well as clinical practitioners and those involved in commercialization, regulatory and entrepreneurial activities.
Book Synopsis Cardiac Regeneration and Repair by : Ren-Ke Li
Download or read book Cardiac Regeneration and Repair written by Ren-Ke Li and published by Elsevier. This book was released on 2014-02-17 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac Regeneration and Repair, Volume Two reviews the use of biomaterials, alone or combined with cell therapy, in providing tissue-engineered constructs to repair the injured heart and prevent or reverse heart failure. Part one explores the variety of biomaterials available for cardiac repair, including nanomaterials and hydrogels. Further chapters explore the use of biomaterials to enhance stem cell therapy for restoring ventricular function and generating stem cell-modified intravascular stents. Part two focuses on tissue engineering for cardiac repair, including chapters on decellularized biologic scaffolds, synthetic scaffolds, cell sheet engineering, maturation of functional cardiac tissue patches, vascularized engineered tissues for in vivo and in vitro applications, and clinical considerations for cardiac tissue engineering. Finally, part three explores vascular remodeling, including chapters highlighting aortic extracellular matrix remodeling, cell-biomaterial interactions for blood vessel formation, and stem cells for tissue-engineered blood vessels. Cardiac Regeneration and Repair, Volume Two is complemented by an initial volume covering pathology and therapies. Together, the two volumes of Cardiac Regeneration and Repair provide a comprehensive resource for clinicians, scientists, or academicians fascinated with cardiac regeneration, including those interested in cell therapy, tissue engineering, or biomaterials. Surveys the variety of biomaterials available for cardiac repair, including nanomaterials and hydrogels. Focuses on tissue engineering for cardiac repair including clinical considerations for cardiac tissue engineering Explores vascular remodeling, highlighting aortic extracellular matrix remodeling, cell-biomaterial interactions for blood vessel formation, and stem cells for tissue-engineered blood vessels
Book Synopsis Cardiovascular Regenerative Medicine by : Vahid Serpooshan
Download or read book Cardiovascular Regenerative Medicine written by Vahid Serpooshan and published by Springer. This book was released on 2019-06-29 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive and up-to-date resource on the use of regenerative medicine for the treatment of cardiovascular disease. It provides a much-needed review of the rapid development and evolution of bio-fabrication techniques to engineer cardiovascular tissues as well as their use in clinical settings. The book incorporates recent advances in the biology, biomaterial design, and manufacturing of bioengineered cardiovascular tissue with their clinical applications to bridge the basic sciences to current and future cardiovascular treatment. The book begins with an examination of state-of-the-art cellular, biomaterial, and macromolecular technologies for the repair and regeneration of diseased heart tissue. It discusses advances in nanotechnology and bioengineering of cardiac microtissues using acoustic assembly. Subsequent chapters explore the clinical applications and translational potential of current technologies such as cardiac patch-based treatments, cell-based regenerative therapies, and injectable hydrogels. The book examines how these methodologies are used to treat a variety of cardiovascular diseases including myocardial infarction, congenital heart disease, and ischemic heart injuries. Finally, the volume concludes with a summary of the most prominent challenges and perspectives on the field of cardiovascular tissue engineering and clinical cardiovascular regenerative medicine. Cardiovascular Regenerative Medicine is an essential resource for physicians, residents, fellows, and medical students in cardiology and cardiovascular regeneration as well as clinical and basic researchers in bioengineering, nanomaterial and technology, and cardiovascular biology.
Book Synopsis Cardiac Tissue Engineering by : Smadar Cohen
Download or read book Cardiac Tissue Engineering written by Smadar Cohen and published by Morgan & Claypool Publishers. This book was released on 2012-10-01 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac tissue engineering aims at repairing damaged heart muscle and producing human cardiac tissues for application in drug toxicity studies. This book offers a comprehensive overview of the cardiac tissue engineering strategies, including presenting and discussing the various concepts in use, research directions and applications. Essential basic information on the major components in cardiac tissue engineering, namely cell sources and biomaterials, is firstly presented to the readers, followed by a detailed description of their implementation in different strategies, broadly divided to cellular and acellular ones. In cellular approaches, the biomaterials are used to increase cell retention after implantation or as scaffolds when bioengineering the cardiac patch, in vitro. In acellular approaches, the biomaterials are used as ECM replacement for damaged cardiac ECM after MI, or, in combination with growth factors, the biomaterials assume an additional function as a depot for prolonged factor activity for the effective recruitment of repairing cells. The book also presents technological innovations aimed to improve the quality of the cardiac patches, such as bioreactor applications, stimulation patterns and prevascularization. This book could be of interest not only from an educational perspective (i.e. for graduate students), but also for researchers and medical professionals, to offer them fresh views on novel and powerful treatment strategies. We hope that the reader will find a broad spectrum of ideas and possibilities described in this book both interesting and convincing. Table of Contents: Introduction / The Heart: Structure, Cardiovascular Diseases, and Regeneration / Cell Sources for Cardiac Tissue Engineering / Biomaterials: Polymers, Scaffolds, and Basic Design Criteria / Biomaterials as Vehicles for Stem Cell Delivery and Retention in the Infarct / Bioengineering of Cardiac Patches, In Vitro / Perfusion Bioreactors and Stimulation Patterns in Cardiac Tissue Engineering / Vascularization of Cardiac Patches / Acellular Biomaterials for Cardiac Repair / Biomaterial-based Controlled Delivery of Bioactive Molecules for Myocardial Regeneration
Book Synopsis Extracellular Matrix-derived Implants in Clinical Medicine by : Daniel L Mooradian
Download or read book Extracellular Matrix-derived Implants in Clinical Medicine written by Daniel L Mooradian and published by Woodhead Publishing. This book was released on 2016-05-18 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extracellular Matrix-Derived Implants in Clinical Medicine comprehensively covers the emergence of tissue engineering and regenerative medicine over the past few decades, along with discussions of continuous funding and research. The book provides a state-of-the-art review of this increasingly important technology and how it is translating from bench to bedside. Part One of the book looks at the historical use of human and animal tissues, focusing on the main application areas, including cardiovascular, hard and soft tissue engineering, and neurological, while Part Two examines the challenges in harvesting, processing, and manufacturing of extracellular matrices, with a final section reviewing the international regulatory environment and economics of tissue-based products. Addresses issues of tissue engineering and regenerative medicine from a biomaterials industry perspective Looks at the historical use of human and animal tissues, focusing on the main application areas, including cardiovascular, hard and soft tissue engineering, and neurological Examines the challenges in harvesting, processing, and manufacturing of extracellular matrices Reviews the international regulatory environment and economics of tissue-based products
Book Synopsis Tissue Engineering for Cardiovascular Regeneration - Stem Cells, Biomaterials, and Animal Preclinical Model by : Haofan Peng (Eric)
Download or read book Tissue Engineering for Cardiovascular Regeneration - Stem Cells, Biomaterials, and Animal Preclinical Model written by Haofan Peng (Eric) and published by . This book was released on 2012 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis addresses some approach for tissue engineering in cardiovascular regeneration. Cell source, biomaterials, ex vivo and in vivo assessment are all discussed step by step. This dissertation provides a good understanding on cardiovascular disease clinically and scientifically. It is essential to understand cardiovascular disease because there are more than 80 million people in USA suffered from cardiovascular disease in 2007. Aging, smoking, diet, lake of exercise, mental stress and genetic inheritance will result in vascular abnormality such as atherosclerosis, stenosis and aneurysm which might eventually lead to stroke or heart attack. Autologous and allogeneic grafts are the most promising solutions for vascular replacement like coronary artery or lower limp bypass but their availability is very limited.^One promising solution to vascular replacement is tissue-engineered vascular grafts for its great potential in customization with autologous cells and biocompatible materials. The major objective to a successful tissue engineered vascular graft is long term patency. Optimization of matrix, stem cell source, surface coating, and physiologically-mimic animal environment, surgically-friendly technique and real time monitoring are all elements of developing compatible, durable and functional vascular grafts. Regardless of significant progress in development of biomaterials and advanced technique to cultivate vascular constructs, cell source remains an important issue. Isolation of cells from patients' vessels not only created a second wound but also is limited by the health and age of the donors. Researchers widely studied different stem cell source including embryonic stem cells, adult stem cells and induced pluripotent stem cells.^We explored the potential of hair-follicle adult mesenchymal stem cells in differentiating toward smooth muscle cells which may provide an accessible autologous cell source for cardiovascular therapy. Hair-follicle smooth muscle cells (HF-SMCs) were isolated from a hair follicle based on tissue specific promoter. These cells expressed similar markers and exhibited similar functionality as vascular smooth muscle. Hair follicle stem cells also can be differentiated into mesenchymal lineages such as adipocytes, chondrocytes and osteoblasts by induction medium condition which need to optimize concentration of serum, growth factors, amino acid, minerals and chemicals to maintain stem cells properties or induce differentiation toward specific lineage. Fibrin hydrogel was widely applied for its biocompatible and biodegradable properties. The vascular constructs showed good compatibility to HF-SMCS and stimulate significant cellular remodeling. The disadvantage is its low mechanical strength which limits fibrin application for arterial reconstruction. Thus, a mechanically-strong and biologically-compatible biomaterial - small intestine submucosa (SIS) was chosen for tissue-engineered arterial substitute. 2 D model was first tested to analyze the compatibility and interaction between cells and matrix. Within two weeks in culture, cells migrated into SIS and secreted decent amount of extra cellular matrix - collagen and elastin, two major extracellular matrix components in the vessel wall. Vascular reactivity increased significantly while mechanical properties were similar to those of native carotid arteries. To make an implantable vascular graft, high concentration fibrin glue was applied to construct a tubular SIS conduit. The SIS grafts can hold pressure up to more than 1000mmHg which is 5 times than the normal physiological level. An implantable vascular graft not only needs to be mechanically-strong, but also need to be anti-thrombogenic. Endothelial cells seeding on biomaterial or modification of lumen on the synthetic grafts can prevent thrombosis in the circulation. Currently, in vitro model systems are inadequate to test elements of thrombogenicity and vascular dynamic functional properties while in vivo implantation is surgically-complicated, labor-intensive and cost-ineffective. To test our vascular grafts and validate the development protocol for cells seeding, we proposed an ex vivo ovine arteriovenous shunt model in which we can test the patency and physical properties of vascular grafts under physiologic conditions. Carotid arteries and jugular veins were collected freshly from the sheep to be compared with SIS grafts. Anti-coagulation properties of grafts were assessed via scanning electron microscopy imaging, en face immunostaining and histology. Seeding endothelial cells in the lumen greatly decreased attachment of thrombotic components. The most significant characteristic of this model is its convenience and accessibility in a suture free and relatively high throughput environment where multiple samples can be stably processed within one animal. After optimizing implantable SIS grafts, we moved further to preclinical animal implantation to test long-term patency of tissue engineered vascular grafts (TEVs). Surgical technique, medication of anticoagulation drug and immunosuppressor are all critical to contribute to a successful implantation. We developed a platform in which the grafts can be implanted easily without damaging risk of endothelium or compromising animal cardiovascular physiology. Animals were followed up and monitored up to 3 month. Pulsatile flow inside grafts was measured by Doppler ultrasound. In angiogram the TEV diameter remained consistent without evidence of dilation or aneurysm. When scarifying animals, the lumen of the grafts remain smooth and shining suggested there was no blood coagulation in the lumen or anastomosis site. No stenosis, graft failures, occlusions or anastomotic complications were observed. Significant cells infiltration was seen in histological analysis as wells as an intact endothelium layer on the lumen. This study suggested an implantable and biocompatible arterial substitute was successfully made by Hair follicle stem cells and clinically-available acellular matrix- small intestinal submucosa for cardiovascular regeneration.
Book Synopsis Advanced Therapies for Cardiac Regeneration by : Valeria Chiono
Download or read book Advanced Therapies for Cardiac Regeneration written by Valeria Chiono and published by Frontiers Media SA. This book was released on 2021-04-13 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Research Topic is organized in the framework of the project BIORECAR (grant number: 772168; http://www.biorecar.polito.it/index.html)
Book Synopsis Cardiac Tissue Engineering by : Smadar Cohen
Download or read book Cardiac Tissue Engineering written by Smadar Cohen and published by Springer Nature. This book was released on 2022-06-01 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac tissue engineering aims at repairing damaged heart muscle and producing human cardiac tissues for application in drug toxicity studies. This book offers a comprehensive overview of the cardiac tissue engineering strategies, including presenting and discussing the various concepts in use, research directions and applications. Essential basic information on the major components in cardiac tissue engineering, namely cell sources and biomaterials, is firstly presented to the readers, followed by a detailed description of their implementation in different strategies, broadly divided to cellular and acellular ones. In cellular approaches, the biomaterials are used to increase cell retention after implantation or as scaffolds when bioengineering the cardiac patch, in vitro. In acellular approaches, the biomaterials are used as ECM replacement for damaged cardiac ECM after MI, or, in combination with growth factors, the biomaterials assume an additional function as a depot for prolonged factor activity for the effective recruitment of repairing cells. The book also presents technological innovations aimed to improve the quality of the cardiac patches, such as bioreactor applications, stimulation patterns and prevascularization. This book could be of interest not only from an educational perspective (i.e. for graduate students), but also for researchers and medical professionals, to offer them fresh views on novel and powerful treatment strategies. We hope that the reader will find a broad spectrum of ideas and possibilities described in this book both interesting and convincing. Table of Contents: Introduction / The Heart: Structure, Cardiovascular Diseases, and Regeneration / Cell Sources for Cardiac Tissue Engineering / Biomaterials: Polymers, Scaffolds, and Basic Design Criteria / Biomaterials as Vehicles for Stem Cell Delivery and Retention in the Infarct / Bioengineering of Cardiac Patches, In Vitro / Perfusion Bioreactors and Stimulation Patterns in Cardiac Tissue Engineering / Vascularization of Cardiac Patches / Acellular Biomaterials for Cardiac Repair / Biomaterial-based Controlled Delivery of Bioactive Molecules for Myocardial Regeneration
Book Synopsis Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix by : Mickey Shah
Download or read book Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix written by Mickey Shah and published by . This book was released on 2018 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: Decellularized porcine myocardium has great potential in serving as a cardiac patch for heart repair after myocardial infarction (MI). However, using full thickness of decellularized porcine myocardium as a cardiac patch may lead to poor viability of the delivered cells due to perfusion limitation and may add an unwanted additional load to the infarcted heart. To address these issues we examined the feasibility of using a thin decellularized porcine myocardium slice (dPMS) as a cardiac patch to deliver therapeutic cells and assess whether this cardiac tissue engineering strategy would improve the function of injured myocardium caused by a MI. We started with obtaining thin dPMSs of various thicknesses by cryosectioning and then checked the effects of thickness on the mechanical properties of dPMS. Adipose derived Stem Cells (ASCs) from rats and pigs were isolated, characterized, and seeded on top of dPMSs with selected thicknesses. The viability, proliferation, infiltration, and differentiation of the seeded rat and pig ASCs were evaluated in vitro. The therapeutic outcomes of dPMS as a cardiac patch (with and without cells) were determined in vivo using a rat MI model. Our results confirmed that the fabricated dPMS can serve as a cell delivery platform to deliver a large amount of cells to injured myocardium with an increased cell retention rate as compared with direct cell injection. Furthermore, when used as a cardiac patch, dPMS integrates with host tissue, promotes host cell infiltration, increases vascularization in the infarcted area, and most importantly enhances cardiac function.
Book Synopsis Cardiac Tissue Engineering by : Milica Radisic
Download or read book Cardiac Tissue Engineering written by Milica Radisic and published by Humana Press. This book was released on 2014-07-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
Book Synopsis Injectable Extracellular Matrix Hydrogels for Cardiac Repair by : Sonya Baigam Seif-Naraghi
Download or read book Injectable Extracellular Matrix Hydrogels for Cardiac Repair written by Sonya Baigam Seif-Naraghi and published by . This book was released on 2012 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiovascular disease is the leading cause of death in the United States and the rest of the western world. Interrupting the progression of negative remodeling after a myocardial infarction (MI) could mitigate the damage caused by a heart attack and preventing the onset of heart failure. Extracellular matrix (ECM) hydrogels have emerged as potential scaffolds for cardiac repair; however, all ECM hydrogels previously explored have been derived from xenogeneic sources, raising concerns regarding immunogenicity, disease transfer, and increased regulation by the FDA. In this dissertation work, a potentially autologous pericardial matrix hydrogel is developed, characterized, and tested. The presence of sulfated sugars in the material led to the hypothesis that heparin-binding growth factors could be sequestered for prolonged delivery and increased effect in vivo. The positive results of this work prompted the exploration of delivering a novel growth factor candidate with the pericardial matrix to evaluate the potential effect on cardiac function post-MI, demonstrating the potential value of this growth factor delivery system in vivo. Though concerns regarding using a xenogeniec material provided the basis for first investigating an autologous option, understanding the effect on the host response is important, prompting evaluation of the inflammatory response to rat myocardial matrix compared to porcine myocardial matrix. Using a non-decellularized myocardial matrix and saline as controls, we sought to understand the inflammatory response to allogeneic and xenogeneic materials in healthy animals. The only signs of chronic inflammation were seen in the non-decellularized positive controls; little difference was noted between the porcine and rat ECM hydrogels, supporting the conclusion that there may be no added value to allogeneic over xenogeneic materials. Exposure to myocardial matrix had no effect on clotting times or platelet activation in human blood and platelet-rich-plasma samples. Direct injection of the ECM hydrogel into the lumen of the left ventricle did not cause notable ischemia, edema, or hemorrhage in peripheral organs. This work establishes the safety and biocompatibility of the myocardial matrix and, taken with recent results from pre-clinical large animal studies, provides a foundation for moving forward with clinical trials.
Book Synopsis Decellularization Methods of Tissue and Whole Organ in Tissue Engineering by : Abdol-Mohammad Kajbafzadeh
Download or read book Decellularization Methods of Tissue and Whole Organ in Tissue Engineering written by Abdol-Mohammad Kajbafzadeh and published by Springer Nature. This book was released on 2021-09-28 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume is the first of a series that introduces safe, feasible, and practical decellularization and recellularization techniques for tissue and organ reconstruction. We have put special emphasis on the research areas most likely to develop well-engineered scaffolds for tissue and organ engineering, while presenting easily applicable bench-to-bedside approaches highlighting the latest technical innovations in the field. This book includes both a fundamental discussion for a broad understanding of the basis of tissue repair and substitution, as well as chapters written by world renowned specialists from 20 countries providing deeper discussions and analysis of related sub disciplines. Within these pages, the reader will find state-of-the-art protocols and current clinical challenges in cell and tissue biology, including accurate and comprehensive information on extracellular matrices, natural biomaterials, tissue dynamics, morphogenesis, stem cells, cellular fate progressions, cell and tissue properties for in-vitro and in-vivo applications. This comprehensive and carefully organized treatise provides a clear framework for graduate students and postdoctoral researchers new to the field, but also for researchers and practitioners looking to expand their knowledge on tissue and organ reconstruction.
Book Synopsis Spotlight on the Heart's Extracellular Matrix by : Karl T. Weber
Download or read book Spotlight on the Heart's Extracellular Matrix written by Karl T. Weber and published by . This book was released on 2000 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Tissue Engineering Strategies for Cardiac Regeneration by : Jennifer R. Hurley
Download or read book Tissue Engineering Strategies for Cardiac Regeneration written by Jennifer R. Hurley and published by . This book was released on 2011 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiovascular disease (CVD) is a significant health burden and is the leading cause of death in the United States. Cardiac pathologies which lead to cardiovascular disease, including myocardial infarction and diabetes, often result in damaged, ischemic and/or fibrotic tissue and would benefit from a therapeutic approach to promote cardiac regeneration. The use of a tissue engineering approach, where the principles of biology and engineering are combined to develop functional tissue substitutes, is a very promising and active field of study. However, cardiac tissue engineering approaches to date have been limited due to 1) insufficient vascularization of engineered tissues (essential for the supply of oxygen, nutrients, and immune cells and waste removal) and 2) impaired regulation of extracellular matrix remodeling and turnover (leading to damaging structural, geometric and functional changes in the heart). Therefore, promoting both vascularization and reparative matrix remodeling is one of the key requirements for successful cardiac regeneration via cardiac tissue engineering approaches. Our long-term goal is to develop a new cardiac tissue engineering approach to treat cardiovascular diseases, including myocardial infarction and diabetic cardiomyopathy, by applying recent advances in nanobiotechnology to modify the microenvironment of heart muscle and promote cardiac regeneration. The studies in this dissertation contribute to this goal by investigating the interactions between cardiac cells, including endothelial cells and fibroblasts, as well as the use of RAD16-II peptide nanofibers and mechanical strain to promote angiogenesis and reparative matrix remodeling by these cells in vitro. The central hypothesis of this research is that RAD16-II peptide nanofibers can be used as a microenvironment for a cardiac tissue engineering approach which promotes cardiac regeneration via revascularization and reparative matrix remodeling by cardiac fibroblasts. The results from this dissertation research help identify the role of fibroblasts in temporal regulation of the angiogenic process and in reparative matrix remodeling. Additionally, the effects of diabetes on the matrix remodeling response by fibroblasts and potential compensatory tissue engineering strategies (i.e. mechanical stretch, peptide nanofiber microenvironment) were investigated as well. The culture system of RAD16-II nanofibers was utilized as both a controlled microenvironment for study and as a system which supports matrix metalloproteinase-mediated extracellular matrix remodeling by cardiac fibroblasts in vitro. The findings of this research will contribute towards developing an optimal microenvironment to enhance cardiac regeneration after injury and for cardiac tissue engineering applications.
Book Synopsis Remodelling of the Cardiac Extracellular Matrix in Ageing and Heart Failure by : Margaux Ann Horn
Download or read book Remodelling of the Cardiac Extracellular Matrix in Ageing and Heart Failure written by Margaux Ann Horn and published by . This book was released on 2010 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of a Naturally Derived Cardiac Extracellular Matrix Hydrogel by : Stephen YuHsiang Lin
Download or read book Characterization of a Naturally Derived Cardiac Extracellular Matrix Hydrogel written by Stephen YuHsiang Lin and published by . This book was released on 2012 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: The extracellular matrix (ECM) is a complex composition of proteins, proteoglycans, and glycosaminoglycans. Biological scaffolds composed of ECM have been used to help repair a variety of tissues in the body. It has been shown that an extracellular matrix material can be generated by decellularizing cardiac tissue and processed into injectable formulations for cardiac tissue engineering. When brought to physiological conditions, the matrix material will self-assemble into a porous hydrogel. The objective of the present study is to prepare the gel form of the myocardial matrix, characterize and tailor material properties of the gel, compare material properties to a human derived myocardial matrix gel, and finally evaluate the ability of the gel to support the growth of smooth muscle cells in vitro. Material properties including viscosity, swelling ratio, gelation kinetics, and stiffness were assessed. The results of the study show that material properties of the myocardial matrix can be tailored by adjusting gelation conditions. Changes in final pH, salt concentration, and protein concentration had effects on viscosity and gelation kinetics but not swelling ratio. This study also shows that there are differences in material properties between that of the porcine myocardial matrix and the human myocardial matrix. Finally the study demonstrated that myocardial matrix supported the adhesion and proliferation of rat aortic smooth muscle cells under static conditions.
