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Expression Profiling In Neuroscience
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Book Synopsis Expression Profiling in Neuroscience by : Yannis Karamanos
Download or read book Expression Profiling in Neuroscience written by Yannis Karamanos and published by Humana Press. This book was released on 2012-01-05 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcriptomics and proteomics, studying the profile of the expression of nucleic acids and proteins respectively, are increasingly applied to gain a mechanistic insight into a wide spectrum of investigation, and the use of expression profiling studies for the central nervous system and brain function aids in the understanding of neurodegenerative disorders and tumor development mechanisms. In Expression Profiling in Neuroscience, expert researchers provide a survey of the most commonly used approaches in the field and scan the different ways of studying the central nervous system/brain environment through expression profiling. The first part addresses the gene expression profiling of the brain at a large scale or to a specific cell type such as blood-brain barrier endothelium. Then, the second part describes the protein expression studies and the different technologies applied. Written for the popular Neuromethods series, chapters include corresponding background information, tested laboratory protocols, and step-by-step methods for reproducible laboratory experiments. Detailed and authoritative, Expression Profiling in Neuroscience presents the state-of-the-art techniques necessary to expand research further into this vital area of study.
Book Synopsis Functional Genomics and Proteomics in the Clinical Neurosciences by : Scott E. Hemby
Download or read book Functional Genomics and Proteomics in the Clinical Neurosciences written by Scott E. Hemby and published by Elsevier. This book was released on 2006-10-09 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses. * Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry* Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies* Use of actual data to illustrate many principles of functional genomics and proteomics. * Introduction to bioinformatics and database management techniques
Book Synopsis Global Gene Expression Profiling of Healthy Human Brain and Its Application in Studying Neurological Disorders by : Simarjeet K. Negi
Download or read book Global Gene Expression Profiling of Healthy Human Brain and Its Application in Studying Neurological Disorders written by Simarjeet K. Negi and published by . This book was released on 2016 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human brain is the most complex structure known to mankind and one of the greatest challenges in modern biology is to understand how it is built and organized. The power of the brain arises from its variety of cells and structures, and ultimately where and when different genes are switched on and off throughout the brain tissue. In other words, brain function depends on the precise regulation of gene expression in its sub-anatomical structures. But, our understanding of the complexity and dynamics of the transcriptome of the human brain is still incomplete. To fill in the need, we designed a gene expression model that accurately defines the consistent blueprint of the brain transcriptome; thereby, identifying the core brain specific transcriptional processes conserved across individuals. Functionally characterizing this model would provide profound insights into the transcriptional landscape, biological pathways and the expression distribution of neurotransmitter systems. Here, in this dissertation we developed an expression model by capturing the similarly expressed gene patterns across congruently annotated brain structures in six individual brains by using data from the Allen Brain Atlas (ABA). We found that 84% of genes are expressed in at least one of the 190 brain structures. By employing hierarchical clustering we were able to show that distinct structures of a bigger brain region can cluster together while still retaining their expression identity. Further, weighted correlation network analysis identified 19 robust modules of coexpressing genes in the brain that demonstrated a wide range of functional associations. Since signatures of local phenomena can be masked by larger signatures, we performed local analysis on each distinct brain structure. Pathway and gene ontology enrichment analysis on these structures showed, striking enrichment for brain region specific processes. Besides, we also mapped the structural distribution of the gene expression profiles of genes associated with major neurotransmission systems in the human. We also postulated the utility of healthy brain tissue gene expression to predict potential genes involved in a neurological disorder, in the absence of data from diseased tissues. To this end, we developed a supervised classification model, which achieved an accuracy of 84% and an AUC (Area Under the Curve) of 0.81 from ROC plots, for predicting autism-implicated genes using the healthy expression model as the baseline. This study represents the first use of healthy brain gene expression to predict the scope of genes in autism implication and this generic methodology can be applied to predict genes involved in other neurological disorders.
Book Synopsis Neural Degeneration and Repair by : Hans Werner Müller
Download or read book Neural Degeneration and Repair written by Hans Werner Müller and published by John Wiley & Sons. This book was released on 2008-09-08 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions from leading scientists around the world, this is the first book focussing on the analysis of nerve cell damage and repair using genomics, transcriptomics, proteomics and systems biology in order to develop novel therapeutic and diagnostic approaches for neural diseases. Following an introduction into the microarray technology in translational neuroscience, the book goes on to look at the use of '-omics' technologies to analyse molecular changes in traumatic injury, neuron degeneration and regeneration, oxidative stress response, neuropathic pain manifestation etc. The work covers central nervous system as well as peripheral nervous system pathologies. This novel approach makes the book an indispensable companion for neurobiologists, neurologists, cell and molecular biologists, geneticists, and analytical chemists.
Book Synopsis Brain Gene Expression Profiling in Mice Selected for Differential Hypnotic Sensitivity to Ethanol by : Erik James MacLaren
Download or read book Brain Gene Expression Profiling in Mice Selected for Differential Hypnotic Sensitivity to Ethanol written by Erik James MacLaren and published by . This book was released on 2005 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Functional Genomics and Proteomics in the Clinical Neurosciences by : Scott E. Hemby
Download or read book Functional Genomics and Proteomics in the Clinical Neurosciences written by Scott E. Hemby and published by Elsevier Science. This book was released on 2006-12-22 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses. * Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry * Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies * Use of actual data to illustrate many principles of functional genomics and proteomics. * Introduction to bioinformatics and database management techniques
Book Synopsis Gene Expression Profiling in the Developing Neural Tube by : Tamar Harel
Download or read book Gene Expression Profiling in the Developing Neural Tube written by Tamar Harel and published by . This book was released on 2007 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microarrays for the Neurosciences by : Daniel H. Geschwind
Download or read book Microarrays for the Neurosciences written by Daniel H. Geschwind and published by MIT Press. This book was released on 2002 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effort to sequence the human genome has generated a new discipline, functional genomics, or the study of the relationship between the genetic code and its biologic potential. Gene expression studies are made possible not only by the decoding of the human genome, but by the development of new technologies. The preeminent technology in this area, DNA microarrays, is helping to revolutionize the field of neuroscience.
Book Synopsis Profiling Gene Expression in the Brain for Insight Into Neurological Disease by : Magdalena Navarro Torres Arpi
Download or read book Profiling Gene Expression in the Brain for Insight Into Neurological Disease written by Magdalena Navarro Torres Arpi and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Gene Expression Profiling of Neural Crest Cells and Developing Schwann Cells by : Johanna Buchstaller
Download or read book Gene Expression Profiling of Neural Crest Cells and Developing Schwann Cells written by Johanna Buchstaller and published by . This book was released on 2004 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Gene Expression Profiling of the Ictal Hippocampus by : Yi Yuen Wang
Download or read book Gene Expression Profiling of the Ictal Hippocampus written by Yi Yuen Wang and published by . This book was released on 2009 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Gene Expression Profiling to Identify Biomarkers of Motor Neuron Disease by :
Download or read book Gene Expression Profiling to Identify Biomarkers of Motor Neuron Disease written by and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Neuroproteomics written by Oscar Alzate and published by CRC Press. This book was released on 2009-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Book Synopsis Expression Profiling and Functional Studies of Non-coding RNAs in the Central Nervous System by : Amy N. Dubinsky
Download or read book Expression Profiling and Functional Studies of Non-coding RNAs in the Central Nervous System written by Amy N. Dubinsky and published by . This book was released on 2011 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: We computationally identified a single nucleotide change from G to A in the 3'UTR of human cFOS 3'UTR which is predicted to be regulated by the brain enriched miRNA-7. A regulatory role for the single nucleotide change in humans (G->A) was assessed by mutating the single nucleotide in the human cFOS 3'UTR (from A->G), as well as by introducing the corresponding human mutation (G->A) into the rhesus and chimpanzee cFOS 3'UTRs. The presence of the A nucleotide in the predicted MRE for miRNA-7 was sufficient to partially abrogate miRNA-7 activity in reporter plasmids. Finally, overexpression of artificial precursor miRNAs in human HEK293 and mouse N2A cell lines confirmed differential targeting of cFOS in human versus mouse cell lines. These data provide evidence for the potential contribution of a single nucleotide change in humans as regards changes in cFOS regulated gene expression. Since cFOS is a transcription factor, downstream affects from altered expression could be significant. Together, this work provides new support for the role of brain enriched miRNAs in the CNS and identifies functional support that their misregulation or altered expression can impact expression of protein coding transcripts in disease brain, and may be relevant to primate brain evolution.
Book Synopsis Gene expression profiling of the response to physiological and environmental stress in motor neurones by : Laura Ferraiuolo
Download or read book Gene expression profiling of the response to physiological and environmental stress in motor neurones written by Laura Ferraiuolo and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Measuring and Correlating Blood and Brain Gene Expression Levels by : Mary Elizabeth Winn
Download or read book Measuring and Correlating Blood and Brain Gene Expression Levels written by Mary Elizabeth Winn and published by . This book was released on 2011 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray-based gene expression profiling is a frequently utilized tool in the search for disease-specific molecular patterns and the development of clinically relevant panels of biomarkers. Although advances in high-throughput gene expression technology make for more reliable and interpretable studies, investigations of living humans are often limited by tissue accessibility. This is especially true for neural-based illnesses, where studies rely heavily on post-mortem brain tissue. As a result, medical researchers have focused on blood, a more easily accessible and clinically obtainable tissue. In this work I explore : 1.) the technical aspects associated with assessing peripheral whole blood gene expression via microarray; and 2.) the biological significance of blood-based gene expression patterns with respect to brain-based gene expression patterns and behavioral phenotypes in mice and humans. I describe the effects of globin reduction on blood-based gene expression in mice by comparing gene expression patterns before and after globin reduction of mouse whole blood (Chapter 2). Globin reduction was found to improve the ability to detect low abundance, biologically relevant genes. I also evaluated globin reduction in the context of human blood and two Illumina gene expression assays : (i) the IVT-based direct hybridization assay; and (ii) the WG-DASL assay (Chapter 4). As in mice, I was able to recapitulate the known benefits of globin reduction in both assays, while WG-DASL appeared to be more sensitive compared to IVT. Lastly, I characterized the correlations between blood gene expression levels and behavioral phenotypes and compared blood gene expression-trait correlations with brain gene expression-trait correlations in respect to neuropsychiatric phenotypes in mice (Chapter 3) and autism in humans (Chapter 5). In both mice and humans, blood was only able to capture a small portion of the associations identified in the brain on an individual gene level. At a pathway level, blood was able to capture a larger portion of the associated brain pathways in humans as compared to mice. I conclude blood gene expression, although it may capture a small portion of the expression patterns associated with 'primary' neural insults, is more likely to capture variation due to 'secondary' perturbations or other biological and environmental insults.
Book Synopsis Expression Profiling of the Parkinsonian Substantia Nigra by : Dawn Duke
Download or read book Expression Profiling of the Parkinsonian Substantia Nigra written by Dawn Duke and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
