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Author : Lukas Brückner
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (982 download)
Download or read book Exploring the Potential of Nonlinear Optical Microscopy with Tailored Femtosecond Pulses written by Lukas Brückner and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Eric Olaf Potma
Publisher : John Wiley & Sons
ISBN 13 : 1119814901
Total Pages : 453 pages
Book Rating : 4.1/5 (198 download)
Download or read book Foundations of Nonlinear Optical Microscopy written by Eric Olaf Potma and published by John Wiley & Sons. This book was released on 2024-03-13 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foundations of Nonlinear Optical Microscopy Concise yet comprehensive resource presenting the foundations of nonlinear optical microscopy Foundations of Nonlinear Optical Microscopy brings together all relevant principles of nonlinear optical (NLO) microscopy, presenting NLO microscopy within a consistent framework to allow for the origin of the signals and the interrelation between different NLO techniques to be understood. The text provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, sensitivity, and imaging contrast. The book also addresses typical questions students ask, and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead. Foundations of Nonlinear Optical Microscopy covers sample topics such as: Light propagation, focusing of light, pulses of light, classical description of light-matter interactions, and quantum mechanical description of light-matter interactions Molecular transitions, selection rules, signal radiation, and detection of light Multi-photon fluorescence and pump-probe microscopy Harmonic generation, sum-frequency generation, and coherent Raman scattering Senior undergraduate and graduate students in chemistry, physics, and biomedical engineering, along with students of electrical engineering and instructors in both of these fields, can use the information within Foundations of Nonlinear Optical Microscopy and the included learning resources to gain a concise yet comprehensive overview of the subject.
Author : Adam Michael Larson
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (69 download)
Download or read book Optical Fiber Based Ultrashort Pulse Multispectral Nonlinear Optical Microscopy written by Adam Michael Larson and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonlinear optical microscopy (NLOM) utilizing femtosecond laser pulses is well suited for imaging living tissues. This work reports on the design and development of an optical fiber based multispectral NLOM developed around a laser generating broadband sub-10-fs pulses. An all-mirror dispersion-compensation setup is used to correct for quadratic and cubic phase distortions induced within the NLOM. Mouse tail tendon was used to characterize sub-10-fs pulses by interferometric autocorrelation. This is an effective method for characterizing dispersion from the optical system, immersion medium, and wet biological sample. The generation of very short autocorrelations demonstrates the ability to compensate for phase distortions within the imaging system and efficient second-harmonic upconversion of the ultrashort pulse spectrum within collagen. Reconstruction of ultrashort pulses at the focal plane of the objective allows the excitation of multiple fluorescent probes simultaneously. Multiple fluorescent probe excitation and spectral discrimination is demonstrated using mixtures of fluorescent dye solutions and an in-vitro angiogenesis model containing human umbilical vein endothelial cells (HUVEC's) expressing multiple fluorescent proteins. Sub-10-fs pulses can be propagated through polarization-maintaining single mode fiber (PMF) for use in NLOM. We demonstrate delivery of near transform-limited, 1 nJ pulses from a Ti:Al2O3 oscillator via PMF to the NLOM focal plane while maintaining 120 nm of bandwidth. Negative group delay dispersion (GDD) introduced to pre-compensate normal dispersion of the optical fiber and microscope optics ensured linear pulse propagation through the PMF. Nonlinear excitation of multiple fluorophores simultaneously and polarization sensitive NLOM imaging using second harmonic generation in collagen was demonstrated using PMF delivered pulses. Two-photon excited fluorescence spectra and second harmonic images taken with and without the fiber indicates that the fiber based system is capable of generating optical signals that are within a factor of two to three of our traditional NLOM.
Author : Kazuyoshi Itoh
Publisher : Wiley
ISBN 13 : 9781118091449
Total Pages : 0 pages
Book Rating : 4.0/5 (914 download)
Download or read book Functional Imaging by Controlled Nonlinear Optical Phenomena written by Kazuyoshi Itoh and published by Wiley. This book was released on 2013-11-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.
Author : Mikio Yamashita
Publisher : Springer
ISBN 13 : 3540271406
Total Pages : 400 pages
Book Rating : 4.5/5 (42 download)
Download or read book Mono-Cycle Photonics and Optical Scanning Tunneling Microscopy written by Mikio Yamashita and published by Springer. This book was released on 2006-04-04 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deals with both the ultrashort laser-pulse technology in the few- to mono-cycle region and the laser-surface-controlled scanning-tunneling microscopy (STM) extending into the spatiotemporal extreme technology. The former covers the theory of nonlinear pulse propagation beyond the slowly-varing-envelope approximation, the generation and active chirp compensation of ultrabroadband optical pulses, the amplitude and phase characterization of few- to mono-cycle pulses, and the feedback field control for the mono-cycle-like pulse generation. In addition, the wavelength-multiplex shaping of ultrabroadband pulses, and the carrier-phase measurement and control of few-cycle pulses are described. The latter covers the CW-laser-excitation STM, the femtosecond-time-resolved STM and atomic-level surface phenomena controlled by femtosecond pulses.
Author : Min Gu
Publisher : Cambridge University Press
ISBN 13 : 0521882400
Total Pages : 245 pages
Book Rating : 4.5/5 (218 download)
Download or read book Femtosecond Biophotonics written by Min Gu and published by Cambridge University Press. This book was released on 2010-05-06 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering key techniques for optical microscopy and micro-fabrication, this book provides the first detailed treatment of femtosecond laser-based biophotonics. After a review of the techniques for nonlinear and multiphoton imaging, applications for laser-based manipulation of micro-particles are introduced. The final chapter focuses on the burgeoning field of femtosecond micro-engineering.
Author : Romedi Selm
Publisher :
ISBN 13 : 9783843906661
Total Pages : 116 pages
Book Rating : 4.9/5 (66 download)
Download or read book Nonlinear Optical Microscopy written by Romedi Selm and published by . This book was released on 2012 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Anthony Chien-der Lee
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (819 download)
Download or read book Development of the Ultrashort Pulse Nonlinear Optical Microscopy Spectral Imaging System written by Anthony Chien-der Lee and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonlinear Optical Microscopy (NLOM) has been shown to be a valuable tool for noninvasive imaging of complex biological systems. An effective approach for multicolor molecular microscopy is simultaneous excitation of multiple fluorophores by broadband sub-10-fs pulses. This dissertation will discuss the development of two spectral imaging systems using the principles of nonlinear optical microscopy for pixel-by-pixel spectral segmentation of multiple fluorescent spectra. The first spectral system is reliant on a fiber-optic cable to transmit fluorescent signal to a spectrometer, while the second is based on a spectrometer with an aberration-corrected concave grating that is directly coupled to the microscope. A photon-counting, 16-channel multianode photomultiplier tube (PMT) is used for both systems. Custom software developed in LabVIEW controls multiple counter cards as well as a field-programmable gate array (FPGA) for 1 Hz acquisition of 256x256x16 spectral images. Biological specimens consisting of multicolor endothelial cells and zebrafish will be used for experimental verification. Results indicate successful spectral segmentation of multiple fluorophores with a decrease in signal-to-noise ratio in the FPGA-based imaging system.
Author : Frank Träger
Publisher : Springer Science & Business Media
ISBN 13 : 3642194095
Total Pages : 1704 pages
Book Rating : 4.6/5 (421 download)
Download or read book Springer Handbook of Lasers and Optics written by Frank Träger and published by Springer Science & Business Media. This book was released on 2012-05-05 with total page 1704 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition features numerous updates and additions. Especially 4 new chapters on Fiber Optics, Integrated Optics, Frequency Combs and Interferometry reflect the changes since the first edition. In addition, major complete updates for the chapters: Optical Materials and Their Properties, Optical Detectors, Nanooptics, and Optics far Beyond the Diffraction Limit. Features Contains over 1000 two-color illustrations. Includes over 120 comprehensive tables with properties of optical materials and light sources. Emphasizes physical concepts over extensive mathematical derivations. Chapters with summaries, detailed index Delivers a wealth of up-to-date references.
Author : Stefan W. Hell
Publisher :
ISBN 13 :
Total Pages : 104 pages
Book Rating : 4.:/5 (437 download)
Download or read book Nonlinear Optical Microscopy written by Stefan W. Hell and published by . This book was released on 1996 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Kseniya S. Shin
Publisher :
ISBN 13 :
Total Pages : 108 pages
Book Rating : 4.:/5 (124 download)
Download or read book Nonlinear Optical Microscopy for Tissue Imaging and Analysis in Anatomic Pathology written by Kseniya S. Shin and published by . This book was released on 2020 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering (SRS) microscopy provides chemical information for imaged tissue at submicron resolution. In the experiments described, I combine SRS with other non-linear microscopies, including second harmonic generation (SHG) microscopy and two-photon fluorescence (TPF) microscopy, to develop a methodology for tissue imaging and analysis with anatomic pathology diagnostic challenges in mind. Employing an interdisciplinary approach in collaboration with experts in the field I investigate the diagnostic potential of a multimodal approach to complex tissues such as brain, breast, and bone, illuminating the immense potential non-linear optical microscopy has to offer to pathologists and clinicians as they strive to improve patient care.
Author : Ilyas Saytashev
Publisher :
ISBN 13 : 9781339981345
Total Pages : 132 pages
Book Rating : 4.9/5 (813 download)
Download or read book Nonlinear Optical Microscopy of Highly Pigmented Tissues written by Ilyas Saytashev and published by . This book was released on 2016 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Haifeng Li
Publisher :
ISBN 13 :
Total Pages : 109 pages
Book Rating : 4.:/5 (748 download)
Download or read book Nonlinear Nanoprobes for Characterizing Ultrafast Optical Near Field written by Haifeng Li and published by . This book was released on 2011 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Costantino De Angelis
Publisher : CRC Press
ISBN 13 : 1351269755
Total Pages : 345 pages
Book Rating : 4.3/5 (512 download)
Download or read book Nonlinear Meta-Optics written by Costantino De Angelis and published by CRC Press. This book was released on 2020-05-20 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses fabrication as well as characterization and modeling of semiconductor nanostructures in the optical regime, with a focus on nonlinear effects. The visible range as well as near and far infrared spectral region will be considered with a view to different envisaged applications. The book covers the current key challenges of the research in the area, including: exploiting new material platforms, fully extending the device operation into the nonlinear regime, adding re-configurability to the envisaged devices and proposing new modeling tools to help in conceiving new functionalities. • Explores several topics in the field of semiconductor nonlinear nanophotonics, including fabrication, characterization and modeling of semiconductor nanostructures in the optical regime, with a focus on nonlinear effects • Describes the research challenges in the field of optical metasurfaces in the nonlinear regime • Reviews the use and achievements of all-dielectric nanoantennas for strengthening the nonlinear optical response • Describes both theoretical and experimental aspects of photonic devices based on semiconductor optical nanoantennas and metasurfaces • Gathers contributions from several leading groups in this research field to provide a thorough and complete overview of the current state of the art in the field of semiconductor nonlinear nanophotonics Costantino De Angelis has been full professor of electromagnetic fields at the University of Brescia since 1998. He is an OSA Fellow and has been responsible for several university research contracts in the last 20 years within Europe, the United States, and Italy. His technical interests are in optical antennas and nanophotonics. He is the author of over 150 peer-reviewed scientific journal articles. Giuseppe Leo has been a full professor in physics at Paris Diderot University since 2004, and in charge of the nonlinear devices group of MPQ Laboratory since 2006. His research areas include nonlinear optics, micro- and nano-photonics, and optoelectronics, with a focus on AlGaAs platform. He has coordinated several research programs and coauthored 100 peer-reviewed journal articles, 200 conference papers, 10 book chapters and also has four patents. Dragomir Neshev is a professor in physics and the leader of the experimental photonics group in the Nonlinear Physics Centre at Australian National University (ANU). His activities span over several branches of optics, including nonlinear periodic structures, singular optics, plasmonics, and photonic metamaterials. He has coauthored 200 publications in international peer-reviewed scientific journals.
Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309059917
Total Pages : 358 pages
Book Rating : 4.3/5 (9 download)
Download or read book Harnessing Light written by National Research Council and published by National Academies Press. This book was released on 1998-09-25 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical science and engineering affect almost every aspect of our lives. Millions of miles of optical fiber carry voice and data signals around the world. Lasers are used in surgery of the retina, kidneys, and heart. New high-efficiency light sources promise dramatic reductions in electricity consumption. Night-vision equipment and satellite surveillance are changing how wars are fought. Industry uses optical methods in everything from the production of computer chips to the construction of tunnels. Harnessing Light surveys this multitude of applications, as well as the status of the optics industry and of research and education in optics, and identifies actions that could enhance the field's contributions to society and facilitate its continued technical development.
Author : Ji-Xin Cheng
Publisher : Elsevier
ISBN 13 : 0323903371
Total Pages : 612 pages
Book Rating : 4.3/5 (239 download)
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Author : Jun Ye
Publisher : Springer Science & Business Media
ISBN 13 : 0387237917
Total Pages : 373 pages
Book Rating : 4.3/5 (872 download)
Download or read book Femtosecond Optical Frequency Comb: Principle, Operation and Applications written by Jun Ye and published by Springer Science & Business Media. This book was released on 2006-06-15 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last few years, there has been a convergence between the fields of ultrafast science, nonlinear optics, optical frequency metrology, and precision laser spectroscopy. These fields have been developing largely independently since the birth of the laser, reaching remarkable levels of performance. On the ultrafast frontier, pulses of only a few cycles long have been produced, while in optical spectroscopy, the precision and resolution have reached one part in Although these two achievements appear to be completely disconnected, advances in nonlinear optics provided the essential link between them. The resulting convergence has enabled unprecedented advances in the control of the electric field of the pulses produced by femtosecond mode-locked lasers. The corresponding spectrum consists of a comb of sharp spectral lines with well-defined frequencies. These new techniques and capabilities are generally known as “femtosecond comb technology. ” They have had dramatic impact on the diverse fields of precision measurement and extreme nonlinear optical physics. The historical background for these developments is provided in the Foreword by two of the pioneers of laser spectroscopy, John Hall and Theodor Hänsch. Indeed the developments described in this book were foreshadowed by Hänsch’s early work in the 1970s when he used picosecond pulses to demonstrate the connection between the time and frequency domains in laser spectroscopy. This work complemented the advances in precision laser stabilization developed by Hall.
