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Exocytosis Methods
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Book Synopsis Exocytosis and Endocytosis by : Andrei I. Ivanov
Download or read book Exocytosis and Endocytosis written by Andrei I. Ivanov and published by Springer Science & Business Media. This book was released on 2008 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, skilled experts provide the most up-to-date, step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. The book is insightful to both newcomers and seasoned professionals. It offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.
Download or read book Exocytosis Methods written by Peter Thorn and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission. The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion. The volume continues with several model systems that are being employed to unravel the complexities of exocytosis. Written for the Neuromethods series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results. Practical and authoritative, Exocytosis Methods seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field.
Book Synopsis Exocytosis and Endocytosis by : Florence Niedergang
Download or read book Exocytosis and Endocytosis written by Florence Niedergang and published by Humana. This book was released on 2021-12-06 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation.
Book Synopsis Exocytosis and Endocytosis by : Andrei I. Ivanov
Download or read book Exocytosis and Endocytosis written by Andrei I. Ivanov and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.
Book Synopsis Endocytosis and Exocytosis by : A.G. Lee
Download or read book Endocytosis and Exocytosis written by A.G. Lee and published by Elsevier. This book was released on 1996-12-17 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 4 of Biomembranes covers endocytosis, exocytosis and related processes. A major role of the plasma membrane is as a permeability barrier, keeping the inside of the cell inside and the outside, outside. Mechanisms must then exist to allow movement of material between the cell and its environment. One mechanism for export from the cell is by exocytosis, a process in which the membranes of secretory vesicles fuse with the plasma membrane releasing the contents of the vesicle into the extracellular medium. The process has been studied in particular depth for the release of neurotransmitters at the synapse. Import into the cell is possible by the process of receptor-mediated endocytosis in which selected plasma membrane proteins are internalizes; when these proteins are receptors for macromolecules, the result is uptake of the macromolecule. Transferring, the low-density lipoprotein, and asialoglycoproteins are all taken up into cells in this way. Phagocytosis, the ingestion of cells and cell fragments by neutrophils and macrophages, also involves receptors - on the phagocytic membrane - of which the best studied are those for the Fc domain of IgG, for the third component of complement, and for the mannose/fructose carbohydrates. Protection of a host against infection can also be achieved by damaging the integrity of the plasma membrane of the invading organism. This is the strategy evolved by the cytotoxic T lymphocytes, which produce a pore-forming toxin, perforin. Volume 4 of Biomembranes explores the structures and mechanisms involved in these biologically and medically important processes.
Book Synopsis Molecular Biology of The Cell by : Bruce Alberts
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development and Application of Analytical Methods for the Analysis of Exocytosis at the Single-cell Level by : Tom L. Colliver
Download or read book Development and Application of Analytical Methods for the Analysis of Exocytosis at the Single-cell Level written by Tom L. Colliver and published by . This book was released on 2000 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Mechanisms of Exocytosis by :
Download or read book Molecular Mechanisms of Exocytosis written by and published by . This book was released on 2007 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exocytosis is a fundamental cellular process that is used by eukaryotic cells to release a variety of biological compounds including peptide hormones and neurotransmitters or to insert specific lipids and proteins in the plasma membrane. In recent years, a multidisciplinary approach promoted an extraordinary progress in the understanding of the molecular mechanisms regulating exocytosis. This led to the discovery of a large number of components belonging to the machinery that governs the fusion of secretory vesicles with plasma membranes in different cell systems, including neuronal and endocrine cells. The basic machinery required for vesicle fusion turned out to be well conserved through evolution from yeast to man. So far, because of the large number of components involved, understanding of the molecular basis of exocytosis has remained the privilege of a relatively small group of specialists. This book, written by recognized experts in the field aims at clarifying for a non-specialist audience the role of the key players in the exocytotic process not only in neuronal and endocrine cells but also in a variety of other relevant cell systems. The book represents a unique collection of up-to-date reviews that will introduce researchers and students to the forefront of this rapidly moving and fascinating field.
Book Synopsis Molecular Mechanisms of Exocytosis by : Romano Regazzi
Download or read book Molecular Mechanisms of Exocytosis written by Romano Regazzi and published by Springer Science & Business Media. This book was released on 2008-05-12 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the role of key components in the exocytotic process, not only in neuronal and endocrine cells but in a variety of other relevant cell systems. So far, because of the large number of components involved, understanding of the molecular basis of exocytosis has remained the privilege of a relatively small group of specialists. The book collects up-to-date reviews from the forefront of this fascinating and rapidly evolving field.
Book Synopsis Pharmacology of Neurotransmitter Release by : Thomas C. Südhof
Download or read book Pharmacology of Neurotransmitter Release written by Thomas C. Südhof and published by Springer Science & Business Media. This book was released on 2007-12-07 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been known for half a century that neurotransmitters are released in preformed quanta, that the quanta represent transmitter-storing vesicles, and that release occurs by exocytosis. The focus of this book is twofold. In the first part, the molecular events of exocytosis are analysed. In the second part of the book, the presynaptic receptors for endogenous chemical signals are presented that make neurotransmitter release a highly regulated process.
Book Synopsis Electroanalytical Methods Of Biological Materials by : Anna Brajter-toth
Download or read book Electroanalytical Methods Of Biological Materials written by Anna Brajter-toth and published by CRC Press. This book was released on 2002-03-21 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text details contemporary electroanalytical strategies of biomolecules and electrical phenomena in biological systems. It presents developments in sequence-specific DNA detection for more efficient medical diagnosis of genetic and infectious diseases and microbial and viral pathogens.
Book Synopsis Mechanisms of Exocytosis, Volume 1152 by : Robert Zorec
Download or read book Mechanisms of Exocytosis, Volume 1152 written by Robert Zorec and published by Wiley-Blackwell. This book was released on 2009-02-09 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exocytosis, a universal process of eukaryotic cells, consisting of the fusion between the vesicle and the plasma membrane is an important topic in cell biology, physiology, biophysics, biochemistry and many other disciplines. The understanding of this rather complex process is essential for the understanding of normal function of unicellular and multicellular organisms from animal to plant kingdoms and in pathological conditions as well.
Book Synopsis Endocytosis, Exocytosis and Vesicle Traffic in Plants by : C. R. Hawes
Download or read book Endocytosis, Exocytosis and Vesicle Traffic in Plants written by C. R. Hawes and published by Cambridge University Press. This book was released on 1991-11-07 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The transport of macromolecules in membrane bounded vesicles is a fundamental process of all eukaryotic cells. The paths taken by this vesicular traffic have been intensively researched in animal cells but are less well characterised in plant cells. Nevertheless, with the development of and combination of techniques in biochemistry, molecular biology and cell biology, progress in the study of plant vesicle traffic has been achieved in recent years. This book attempts to highlight the recent advances made and to explore avenues for future research. The book opens with a general overview of vesicular traffic both in animal and plant cells. This is followed by a more detailed consideration of higher plant coated vesicles, endocytosis, exocytosis and mechanisms of vesicle traffic and transport in cells. The biochemistry, cell biology and molecular biology of each is considered and particular attention is given to those specialised cell systems in which vesicle traffic is of particular importance. This book brings together expertise from a wide range of research disciplines involved in the study of vesicle traffic in plants, and, as such, provides a unique synthesis from which the research can move forward. It will be of general interest to those involved in the study of vesicle traffic in both plant and animal cells.
Book Synopsis Biology for AP ® Courses by : Julianne Zedalis
Download or read book Biology for AP ® Courses written by Julianne Zedalis and published by . This book was released on 2017-10-16 with total page 1923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences.
Book Synopsis Concepts of Biology by : Samantha Fowler
Download or read book Concepts of Biology written by Samantha Fowler and published by . This book was released on 2018-01-07 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.
Book Synopsis Exocytosis from Molecules to Cells by : Arun Anantharam
Download or read book Exocytosis from Molecules to Cells written by Arun Anantharam and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is divided into three sections: 1) components of exocytotic pathways; 2) biophysical insights from synthetic and reconstituted systems; and, 3) physiological systems of exocytosis.
Book Synopsis Single Cell Assays of Exocytosis by : Peng Chen
Download or read book Single Cell Assays of Exocytosis written by Peng Chen and published by . This book was released on 2002 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells package signaling molecules (e.g. catecholamine and neurotransmitter) within membrane-encased vesicles. Upon cell stimulation, a rise in intracellular Ca [superscript 2+] concentration triggers the fusion of vesicles with the cell's outer membrane and release of contents into extracellular space in a process called exocytosis. Understanding the process whereby cells transduce an external signal to a secretory response has been an important topic of research of many years. This thesis aims at improving current techniques and developing new techniques for single cell assays of exocytosis. Membrane capacitance measurements of the voltage-clamped cell membrane have revolutionized the studies of exocytosis with unprecedented resolution, because changes in membrane surface area accompany fusion of secretory vesicles with the plasma membrane. Our noise study predicts the theoretical limits of capacitance measurements in the whole-cell configuration, and provides guidelines to choose optimal experimental conditions. Membrane conductance changes often occur concurrently with the stimulus used to evoke exocytosis and can lead to unreliable estimation of membrane capacitance. To measure membrane capacitance faithfully even in presence of large linear (e.g. CFTR current) or non-linear (Ca [superscript 2+] current) ionic currents, we have developed a new cell model to describe "non-ideal" membrane properties and new approaches for estimating membrane capacitance. Electrochemical detection of secretion with micro-carbon fiber microelectrodes has been used as a complementary approach to capacitance measurement to study exocytosis. However, carbon fibers can only capture 2%-15% of total cell secretion due to its planar geometry. We have fabricated picoliter-sized electrochemical electrodes/wells on silicon chips for measuring catecholamine release from individual cells with millisecond resolution. Each well-electrode roughly conforms the shape of the cell in order to capture a large fraction of released catecholamine. By applying engineering principles and techniques for biological studies, this work should advance the electrophysiological assays of exocytosis and lead to a better understanding of its mechanism and regulation.
