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Download or read book Enzyme Kinetics and Mechanism Part D written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Academic Press
ISBN 13 : 9780121821500
Total Pages : 0 pages
Book Rating : 4.8/5 (215 download)
Download or read book Enzyme Kinetics and Mechanism, Part D: Developments in Enzyme Dynamics written by and published by Academic Press. This book was released on 1995-03-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: General Description of the Volume: This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Author :
Publisher : Academic Press
ISBN 13 : 0123847443
Total Pages : 703 pages
Book Rating : 4.1/5 (238 download)
Download or read book Contemporary Enzyme Kinetics and Mechanism written by and published by Academic Press. This book was released on 2009-10-24 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis
Author :
Publisher : Elsevier
ISBN 13 : 0080496660
Total Pages : 494 pages
Book Rating : 4.0/5 (84 download)
Download or read book Enzyme Kinetics and Mechanisms, Part E, Energetics of Enzyme Catalysis written by and published by Elsevier. This book was released on 1999-09-06 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Author : Daniel L. Purich
Publisher : Elsevier
ISBN 13 : 0123809258
Total Pages : 920 pages
Book Rating : 4.1/5 (238 download)
Download or read book Enzyme Kinetics: Catalysis and Control written by Daniel L. Purich and published by Elsevier. This book was released on 2010-06-16 with total page 920 pages. Available in PDF, EPUB and Kindle. Book excerpt: Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions
Author : Daniel L. Purich
Publisher : Elsevier
ISBN 13 : 0080489389
Total Pages : 551 pages
Book Rating : 4.0/5 (84 download)
Download or read book Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates written by Daniel L. Purich and published by Elsevier. This book was released on 2002-11-04 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates
Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
ISBN 13 : 0306480255
Total Pages : 237 pages
Book Rating : 4.3/5 (64 download)
Download or read book Enzyme Kinetics and Mechanisms written by Kenneth B. Taylor and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.
Author : Daniel L. Purich
Publisher : Academic Press
ISBN 13 : 1483214222
Total Pages : 567 pages
Book Rating : 4.4/5 (832 download)
Download or read book Contemporary Enzyme Kinetics and Mechanism written by Daniel L. Purich and published by Academic Press. This book was released on 1983-01-01 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.
Author : Vern L Schramm (Ed)
Publisher :
ISBN 13 :
Total Pages : 768 pages
Book Rating : 4.:/5 (78 download)
Download or read book Enzyme Kinetics and Mechanism written by Vern L Schramm (Ed) and published by . This book was released on 1999 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : P. Boon Chock
Publisher : Springer Science & Business Media
ISBN 13 : 1461237440
Total Pages : 441 pages
Book Rating : 4.4/5 (612 download)
Download or read book Enzyme Dynamics and Regulation written by P. Boon Chock and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in concepts and techniques have brought enzyme research to a changing yet exciting stage. Enzymes have served as indispensable tools in the phenomenal rise of molecular biology, and the resultant biotechnology thrusts enzymes to new heights and territories. This volume, the proceedings of a recent symposium on the Dynamics of Soluble and Immobilized Enzyme Systems, provides a current overview of the field to help scientists utilize long-established and newly acquired information.
Author :
Publisher : Academic Press
ISBN 13 : 0123860040
Total Pages : 369 pages
Book Rating : 4.1/5 (238 download)
Download or read book Biothermodynamics written by and published by Academic Press. This book was released on 2011-02-16 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. * Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules * Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research * Offers step-by-step lab instructions, including necessary equipment, from a global research community
Author :
Publisher : Elsevier
ISBN 13 : 0080522610
Total Pages : 379 pages
Book Rating : 4.0/5 (85 download)
Download or read book Liposomes, Part D written by and published by Elsevier. This book was released on 2004-07-17 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part D is a continuation of previous Methods in Enzymology Liposome volumes A, B, and C. Covers antibody or ligand targeted liposomes; environment sensitive liposomes; liposomal oligonucleotides; liposomes in vivo
Author :
Publisher : Elsevier
ISBN 13 : 0080497217
Total Pages : 527 pages
Book Rating : 4.0/5 (84 download)
Download or read book Numerical Computer Methods, Part D written by and published by Elsevier. This book was released on 2004-05-25 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. Selected Contents: Prediction of protein structure Modeling and studying proteins with molecular dynamics Statistical error in isothermal titration calorimetry Analysis of circular dichroism data Model comparison methods
Author : Jo M. Holt
Publisher : Elsevier
ISBN 13 : 0080497179
Total Pages : 315 pages
Book Rating : 4.0/5 (84 download)
Download or read book Energetics of Biological Macromolecules, Part D written by Jo M. Holt and published by Elsevier. This book was released on 2004-04-02 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed. *Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis
Author : Sankar Adhya
Publisher : Elsevier
ISBN 13 : 9780080522609
Total Pages : 674 pages
Book Rating : 4.5/5 (226 download)
Download or read book RNA Polymerase and Associated Factors written by Sankar Adhya and published by Elsevier. This book was released on 2003-12-18 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Models and methods for studying polymerase translocation * Assay for movements of RNA polymerase along DNA * Engineering of elongation complexes of bacterial and yeast RNA polymerases
Author :
Publisher : Elsevier
ISBN 13 : 0080497039
Total Pages : 553 pages
Book Rating : 4.0/5 (84 download)
Download or read book Nitric Oxide, Part D written by and published by Elsevier. This book was released on 2002-12-09 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Presents information on nitrosothiols and nitric oxide in cell signaling Details nitric oxide and mitochondrial functions Explains nitric oxide synthases
Author : Nooralabettu Krishna Prasad
Publisher : PHI Learning Pvt. Ltd.
ISBN 13 : 8120342399
Total Pages : 373 pages
Book Rating : 4.1/5 (23 download)
Download or read book Enzyme Technology : Pacemaker of Biotechnology written by Nooralabettu Krishna Prasad and published by PHI Learning Pvt. Ltd.. This book was released on 2011-04 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keeping in view the well-established place of enzymes in the field of biotechnology and the recent development in biotech industries, this comprehensive and well-written textbook presents the fundamental concepts of enzyme technology, emphasizing the practical and economic aspects of enzyme usage. Beginning with an overview of enzymes giving insights into the physicochemical properties, classifications, sources, mechanisms and characteristics of enzymes, the text discusses the enzyme kinetics in detail. It furnishes a great deal of information on potential of enzymes for their commercial exploitation. The text then goes on to describe the biotechnical significance of enzymes with their applications in the fields of food and pharmaceutical industries. The text is supported by a large number of solved examples and illustrative diagrams. Primarily designed for undergraduate and postgraduate students of biotechnology and biochemical engineering, the book will also be useful to professionals, researchers and entrepreneurs. KEY FEATURES: Written in an easy-to-understand style. Provides simple, clear and authoritative guide to the principles and scope of enzymes in biotechnology. Includes chapter-end review questions based on recently appeared university question papers.
