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Author : Arthur Kornberg
Publisher :
ISBN 13 :
Total Pages : 128 pages
Book Rating : 4.:/5 (36 download)
Download or read book Enzymatic Synthesis of DNA. written by Arthur Kornberg and published by . This book was released on 1961 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jesús Fernández Lucas
Publisher : John Wiley & Sons
ISBN 13 : 3527343768
Total Pages : 348 pages
Book Rating : 4.5/5 (273 download)
Download or read book Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives written by Jesús Fernández Lucas and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of innovative tools for creative nucleic acid chemists that open the door to novel probes and therapeutic agents Nucleic acids continue to gain importance as novel diagnostic and therapeutic agents. With contributions from noted scientists and scholars, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practical reference that includes a wide range of approaches for the synthesis of designer nucleic acids and their derivatives. The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications. This important book: -Presents a timely and topical guide to the synthesis of designer nucleic acids and their derivatives -Addresses the growing market for nucleotide-derived pharmaceuticals used as anti-infectives and chemotherapeutic agents, as well as fungicides and other agrochemicals. -Covers novel methods and the most recent trends in the field -Contains contributions from an international panel of noted scientistics Written for biochemists, medicinal chemists, natural products chemists, organic chemists, and biotechnologists, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practice-oriented guide that reviews innovative methods for the enzymatic as well as non-enzymatic synthesis of nucleic acid species.
Author : Kazuhiko Nakatani
Publisher : Springer
ISBN 13 : 3319271113
Total Pages : 280 pages
Book Rating : 4.3/5 (192 download)
Download or read book Modified Nucleic Acids written by Kazuhiko Nakatani and published by Springer. This book was released on 2016-04-04 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.
Author : Arthur 1918- Kornberg
Publisher : Hassell Street Press
ISBN 13 : 9781013413988
Total Pages : 128 pages
Book Rating : 4.4/5 (139 download)
Download or read book Enzymatic Synthesis of DNA written by Arthur 1918- Kornberg and published by Hassell Street Press. This book was released on 2021-09-09 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work has been selected by scholars as being culturally important and is part of the knowledge base of civilization as we know it. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. To ensure a quality reading experience, this work has been proofread and republished using a format that seamlessly blends the original graphical elements with text in an easy-to-read typeface. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.
Author : Adolphe Appia
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (959 download)
Download or read book Enzymatic Synthesis of DNA. written by Adolphe Appia and published by . This book was released on 1961 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Saran Narang
Publisher : Elsevier
ISBN 13 : 0323158889
Total Pages : 254 pages
Book Rating : 4.3/5 (231 download)
Download or read book Synthesis And Applications Of DNA And RNA written by Saran Narang and published by Elsevier. This book was released on 2012-12-02 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesis and Applications of DNA and RNA discusses the significant contributions in the development of synthetic routes to DNA and RNA. This book contains nine chapters that describe the complexities in the chemistry and biology of DNA and RNA. After briefly dealing with the various stages of development in the chemical synthesis of polynucleotides, this book goes on presenting the DNA synthesis on solid supports and through the phosphoramidite method on silica supports. The discussions then shift to the chemical-enzymatic synthesis of expressed genes; the biochemical aspects of chemical syntheses of oligoribonucleotides; and the methods of rapid DNA and RNA sequence analysis. A chapter specifically tackles the protocols of DNA synthesis using double-stranded plasmid DNA as a template. The final chapter deals with the use of oligonucleotides for the identification and isolation of specific gene sequences. This chapter also covers the use oligonucleotides in the detection of human genetic diseases. Biologists, geneticists, and researchers interested in DNA and RNA synthesis will find this work invaluable.
Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Aaron M. Leconte
Publisher :
ISBN 13 :
Total Pages : 414 pages
Book Rating : 4.:/5 (763 download)
Download or read book Expanding the Scope of the Enzymatic Synthesis of DNA for written by Aaron M. Leconte and published by . This book was released on 2009 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA polymerases synthesize DNA, the essential biomolecule responsible for encoding the complex information necessary for life, with remarkable efficiency and fidelity. Chemists and biologists have exploited this extraordinary enzyme to develop a wide range of biotechnological tools, including but certainly not limited to PCR and Sanger sequencing. While these landmark applications continue to be relied upon to this day, they represent only a small fraction of the possible uses of these enzymes. Here, I detail my work to expand the scope of the enzymatic synthesis of DNA in two distinct fields. Chapters 2-5 detail efforts to identify replicable candidate unnatural base pairs to expand the genetic alphabet from the natural four base code to an expanded six base code. Using a wide range of techniques, including chemical synthesis, directed evolution of DNA polymerases, and screening based methodologies, the d 5SICS:d MMO2 base pair is identified, resulting in our strongest candidate base pair identified to date. Chapter 6 details the use of an activity based phage display system to identify a DNA polymerase that possesses improved recognition of substrates applicable in next generation sequencing applications.
Author : Anna Baccaro
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (128 download)
Download or read book Enzymatic Synthesis of Functionalized DNA written by Anna Baccaro and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Samson T. Jacob
Publisher : CRC Press
ISBN 13 : 1351088750
Total Pages : 359 pages
Book Rating : 4.3/5 (51 download)
Download or read book Enzymes of Nucleic Acid Synthesis and Modification written by Samson T. Jacob and published by CRC Press. This book was released on 2018-01-18 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First volume gives an overview of the enzymes involved in DNA synthesis and modification; the second volume deals with the RNA-enzymes. Although the major emphasis of the book is on eukaryotic enzymes, a separate chapter dealing with prokaryotic DNA repair enzymes has been included to discuss the major advances in this field in recent years. There are two separate chapters on RNA polymerases to provide a comprehensive coverage of the enzymes from lower eukaryotes, plants and higher eukaryotes.
Author : Hans Günter Gassen
Publisher :
ISBN 13 :
Total Pages : 268 pages
Book Rating : 4.:/5 (45 download)
Download or read book Chemical and Enzymatic Synthesis of Gene Fragments written by Hans Günter Gassen and published by . This book was released on 1982 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preface: In the past the chemical and enzymatic synthesis of oligonucleotides of defined sequence had to be left to a few experts. Now, however, with the triester approach, the phosphite method and the solid-support techniques gene fragment synthesis has turned into an easy procedure even for a non-chemist. Due to the elegant chemistry involved, all methods work without sophisticated equipment and are prone to mechanisation and eventual automation. It is hoped that combined chemical-encymatic gene synthesis may become a standard technique in a molecular biology laboratory, such as DNA sequencing or in-vitro recombination of nucleic acides. We omitted chemical RNA synthesis, since this field is developing so rapidly at the moment that one has to refer to the original publications. However, we included enzymatic synthesis of RNA fragments, procedures which already have obtained a high degree of standardisation. Most of the contributions are revised versions of the protocols supplied for the EMBO sponsored course on "Automated Chemical and Enzymic Gene Synthesis", held in Darmstadt, March 21 to April 3, 1982. The protocols were improved on the basis of the experience of 30 student scientists with chemical, biological or medical backgrounds. Previously omitted procedures, such as the wandering spot method for oligonucleotide analysis, were included. In editing the manuscript we encountered problems with the nomenclature of nucleic acid components. In unambiquous cases we favoured a simple description, hoping for example, that oligodeoxynucleotide is always understood to mean oligo-2'-deoxyribonucleotide. This book aims to provide those interested in DNA/RNA research with state-of-the-art methods in the synthesis, purification, and analysis of DNA and RNA fragments. The editors wish to thank the authors for their efforts in preparing manuscripts from the the revised laboratory protocols. We gratefully acknowledge the skill and the patience of Mrs. E. Ronnfeldt in typing the manuscripts. We express our thanks to Verlag Chemie for the friendly and very efficient cooperation.--H.G. Gassen A. Lang--Darmstadt, in July 1982.
Author : Mohammad Mehedi Masud
Publisher : LAP Lambert Academic Publishing
ISBN 13 : 9783659188435
Total Pages : 124 pages
Book Rating : 4.1/5 (884 download)
Download or read book Enzymatic Synthesis of Modified DNA by PCR and Its Application written by Mohammad Mehedi Masud and published by LAP Lambert Academic Publishing. This book was released on 2012 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thymidine analogue bearing a methyl ester at the C5 position were synthesized. Thermophilic family B DNA polymerases accepted these analogues as substrate to form the corresponding PCR product. Modified DNA containing this analogue were prepared by PCR with KOD Dash DNA polymerase and were further post-modified to form the corresponding derivatized DNA. These DNA could act as a template for PCR and could accept natural TTP or modified analogue as substrate. The thymidine analogue with an amino-linker was also reacted with fluorescein isothiocyanate, yielding a new analogue which was used as a substrate for PCR to get the corresponding fluorescent labeled DNA. Finally, a novel modified DNA aptamer for sialyllactose that is ubiquitous component of the cell surface responsible for the infections of several viruses was produced by using SELEX method with strong binding ability.
Author : Piet Herdewijn
Publisher : Springer Science & Business Media
ISBN 13 : 1592598234
Total Pages : 436 pages
Book Rating : 4.5/5 (925 download)
Download or read book Oligonucleotide Synthesis written by Piet Herdewijn and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Author : Michael John Chamberlin
Publisher :
ISBN 13 :
Total Pages : 474 pages
Book Rating : 4.F/5 ( download)
Download or read book Enzymatic Synthesis of Ribonucleic Acid written by Michael John Chamberlin and published by . This book was released on 1963 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher : Academic Press
ISBN 13 : 0128170964
Total Pages : 380 pages
Book Rating : 4.1/5 (281 download)
Download or read book Enzymatic Polymerizations written by and published by Academic Press. This book was released on 2019-10-14 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Polymerizations, Volume 627 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Enzymatic Polymerizations
Author :
Publisher : Ardent Media
ISBN 13 :
Total Pages : 8 pages
Book Rating : 4./5 ( download)
Download or read book The Life Sciences written by and published by Ardent Media. This book was released on with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Xiaomei Ren
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (16 download)
Download or read book Enzymatic Synthesis of Chemically Modified DNA for Analytical and Diagnostic Applications written by Xiaomei Ren and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
