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Electron Microscopy At Molecular Dimensions
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Book Synopsis Electron Microscopy at Molecular Dimensions by : W. Baumeister
Download or read book Electron Microscopy at Molecular Dimensions written by W. Baumeister and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: If, ten years ago, one had been asked to comment on the prospects of peering into the fmest details of biomolecular organization, most electron microscopists would, I suppose at least, have been quite en thusiastic. When, during the early seventies, several groups were success ful in visualizing single heavy atoms, which undoubtedly was a techni cal triumph, this prompted the most sanguine expectations among bi ologists. In the following years, however, it began to transpire that radiation damage might impose limitations preventing us from taking full advantage of these exciting instrumental feasibilities. Fortunately, the radiation damage nightmare did no paralyze further activities, and it was in particular the work on the purple membrane which, brilliant ly exploiting the redundancy stratagem, revealed exhilarating new perspectives. Now, almost five years later, it seemed timely and appro priate to organize an international symposium to discuss and weight recent activities and current trends in "molecular microscopy". In planning this symposium, we selected topics according to our view of what is important or will deserve more attention in the near future. Taking into consideration suggestions made by the invited participants, some supplementary aspects were included; as a conse quence, the program developed somewhat beyond the scope as adum brated by the original title of this meeting (Regular 2-D Arrays of Biomacromolecules: Structure Determination and Assembly). As the meeting was organized, we had three morning sessions aimed at reflecting the "State ofthe Art".
Book Synopsis Electron Microscopy at Molecular Dimensions by :
Download or read book Electron Microscopy at Molecular Dimensions written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Unconventional Electron Microscopy for Molecular Structure Determination by : W. Hoppe
Download or read book Unconventional Electron Microscopy for Molecular Structure Determination written by W. Hoppe and published by Springer-Verlag. This book was released on 2013-07-02 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Generally it is not sufficiently appreciated that electron microscopy is in fact a diffraction method. In essential aspects electron microscopes are more closely related to X-ray diffracto· meters than to light microscopes. In electron microscopes monochromatized radiation and coherent illumination (never used in light microscopy) correspond in X-ray diffractometers to the primary beam with a small divergence. Imaging ina general sense can take place in interference experiments between a primary beam and a scattered beam, or between diffe rent deflected scattered beams. This leads to the realization of an old dream in diffracto metry, namely to a general experimental solution of the "phase problem". The most im pressive analogy, however, concerns the potential of the electron microscope as a tool for structure determination (where the radiation wavelenght is smaller than the atomic distan ces). It was therefore considered timely to treat this topic in this series. It was a fortunate cioncidence that in 1976 a Workshop on "Unconventional Electron Microscope Methods for the Investigation of Molecular Structures" (sponsored by the European Molecular Biology Organisation, the Deutsche Forschungsgemeinschaft and the Max-Planck-Gesell schaft) took place, and that most speakers presenting introductory lectures agreed to publish their contributions in an expanded version in this volume. This volume is thus not a symposium report in the usual sense since it contains the majority of these introductory lectures only.
Book Synopsis Electron Microscope Studies on the Molecular Dimensions of Some Insect Secreted Fibrous Proteins by : Noel Edward Flower
Download or read book Electron Microscope Studies on the Molecular Dimensions of Some Insect Secreted Fibrous Proteins written by Noel Edward Flower and published by . This book was released on 1967 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :M. A. Nasser Hajibagheri Publisher :Springer Science & Business Media ISBN 13 :1592592015 Total Pages :292 pages Book Rating :4.5/5 (925 download)
Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri
Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Book Synopsis Three-Dimensional Electron Microscopy of Macromolecular Assemblies by : Joachim Frank
Download or read book Three-Dimensional Electron Microscopy of Macromolecular Assemblies written by Joachim Frank and published by Oxford University Press. This book was released on 2006-02-02 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
Book Synopsis The Visualization of Individual Lipoprotein Macromolecules in the Electron Microscope by : Thomas L. Hayes
Download or read book The Visualization of Individual Lipoprotein Macromolecules in the Electron Microscope written by Thomas L. Hayes and published by . This book was released on 1957 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Scanning Electron Microscopy for the Life Sciences by : Heide Schatten
Download or read book Scanning Electron Microscopy for the Life Sciences written by Heide Schatten and published by Cambridge University Press. This book was released on 2013 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.
Book Synopsis On the Correlation Between Micropore Distribution Obtained from Molecular Probes and from High Resolution Electron Microscopy by :
Download or read book On the Correlation Between Micropore Distribution Obtained from Molecular Probes and from High Resolution Electron Microscopy written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: By using immersion calorimetry with liquids of various molecular dimensions, the distributions of the micropore sizes have been obtained for a number of active carbons, for the range 0.3 to 0.75 nm. These carbons have also been examined by high-resolution electron microscopy, the results from both techniques being displayed in the form of histograms. The agreement between the two methods appears to be good and provides possible evidence of the reduced interaction radius of adsorbed molecules and of exfoliation.
Book Synopsis Three-Dimensional Electron Microscopy of Macromolecular Assemblies by : Frank Joachim
Download or read book Three-Dimensional Electron Microscopy of Macromolecular Assemblies written by Frank Joachim and published by Elsevier. This book was released on 1996-01-24 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods.Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins
Book Synopsis Electron Microscopy of Molecular and Atom-Scale Mechanical Behavior, Chemistry and Structure: Volume 839 by : Materials Research Society. Meeting
Download or read book Electron Microscopy of Molecular and Atom-Scale Mechanical Behavior, Chemistry and Structure: Volume 839 written by Materials Research Society. Meeting and published by . This book was released on 2005-06-15 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: The MRS Symposium Proceeding series is an internationally recognised reference suitable for researchers and practitioners. This book, first published in 2005, showcases how electron microscopy is applied to materials problems and to encourage ideas from both the solid-state and biological communities.
Book Synopsis Electron Microscopy of Molecular and Atom-Scale Mechanical Behavior, Chemistry and Structure: by : David C. Martin
Download or read book Electron Microscopy of Molecular and Atom-Scale Mechanical Behavior, Chemistry and Structure: written by David C. Martin and published by Cambridge University Press. This book was released on 2014-06-05 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the imaging and spectroscopy of individual dopant atoms and clusters buried inside a semiconductor host, to the three-dimensional tomography of nanoparticles, virii, and biological structures and the in situ observations of nano-mechanical deformation and electrodeposition, advances in instrumentation and algorithms have dramatically changed the field of electron microscopy. Early results in sub-angstrom resolution and millivolt spectroscopy are now being applied to materials problems, and initiatives in aberration-corrected instruments should make these available to the wider community. This book, first published in 2005, showcases how electron microscopy is applied to materials problems and to encourage ideas from both the solid-state and biological communities. Topics include: atomic and subatomic imaging and spectroscopy; electron energy-loss spectroscopy for sub-nanometer chemical and optical properties; three-dimensional nanoscale characterization; quantitative electron microscopy - holography, dopant profiling and diffraction; imaging individual structures and defects in bio- and nonomaterials and in situ microscopy of deformation and growth (even in liquids).
Book Synopsis Electron Microscopy In Material Science by : U Valdre
Download or read book Electron Microscopy In Material Science written by U Valdre and published by Elsevier. This book was released on 2012-12-02 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy in Material Science covers the proceedings of the International School of Electron Microscopy held in Erice, Itsaly, in 1970. The said conference is intended to the developments of electron optics and electron microscopy and its applications in material science. The book is divided into four parts. Part I discusses the impact of electron microscopy in the science of materials. Part II covers topics such as electron optics and instrumentation; geometric electron optics and its problems; and special electron microscope specimen stages. Part III explains the theory of electron diffraction image contrast and then elaborates on related areas such as the application of electron diffraction and of electron microscopy to radiation; computing methods; and problems in electron microscopy. Part IV includes topics such as the transfer of image information in the electron microscope; phase contrast microscopy; and the magnetic phase contrast. The text is recommended for electron microscopists who are interested in the application of their field in material science, as well as for experts in the field of material science and would like to know about the importance of electron microscopy.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioimaging written by Douglas E. Chandler and published by Jones & Bartlett Publishers. This book was released on 2009 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
Book Synopsis 4D Electron Microscopy by : Ahmed H. Zewail
Download or read book 4D Electron Microscopy written by Ahmed H. Zewail and published by World Scientific. This book was released on 2010 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structural phase transitions, mechanical deformations, and the embryonic stages of melting and crystallization are examples of phenomena that can now be imaged in unprecedented structural detail with high spatial resolution, and ten orders of magnitude as fast as hitherto. No monograph in existence attempts to cover the revolutionary dimensions that EM in its various modes of operation nowadays makes possible. The authors of this book chart these developments, and also compare the merits of coherent electron waves with those of synchrotron radiation. They judge it prudent to recall some important basic procedural and theoretical aspects of imaging and diffraction so that the reader may better comprehend the significance of the new vistas and applications now afoot. This book is not a vade mecum - numerous other texts are available for the practitioner for that purpose.
Book Synopsis Computer Processing of Electron Microscope Images by : P. W. Hawkes
Download or read book Computer Processing of Electron Microscope Images written by P. W. Hawkes and published by Springer. This book was released on 2011-12-27 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Towards the end of the 1960s, a number of quite different circumstances combined to launch a period of intense activity in the digital processing of electron micro graphs. First, many years of work on correcting the resolution-limiting aberrations of electron microscope objectives had shown that these optical impediments to very high resolution could indeed be overcome, but only at the cost of immense exper imental difficulty; thanks largely to the theoretical work of K. -J. Hanszen and his colleagues and to the experimental work of F. Thon, the notions of transfer func tions were beginning to supplant or complement the concepts of geometrical optics in electron optical thinking; and finally, large fast computers, capable of manipu lating big image matrices in a reasonable time, were widely accessible. Thus the idea that recorded electron microscope images could be improved in some way or rendered more informative by subsequent computer processing gradually gained ground. At first, most effort was concentrated on three-dimensional reconstruction, particu larly of specimens with natural symmetry that could be exploited, and on linear operations on weakly scattering specimens (Chap. l). In 1973, however, R. W. Gerchberg and W. O. Saxton described an iterative algorithm that in principle yielded the phase and amplitude of the electron wave emerging from a strongly scattering speci men.
