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Effects Of High Intensity Interval Training On Muscle Oxidative Metabolism In Young Men
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Book Synopsis Effects of High-intensity Interval Training on Muscle Oxidative Metabolism in Young Men by : Ryan Godsk Larsen
Download or read book Effects of High-intensity Interval Training on Muscle Oxidative Metabolism in Young Men written by Ryan Godsk Larsen and published by . This book was released on 2011 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to supply cellular energy in the form of adenosine triphosphate (ATP) by oxidative phosphorylation is critical to maintaining muscle function and health. While conventional endurance exercise training has proven effective at increasing the maximal capacity for oxidative phosphorylation (Vmax), it is not known whether exercise training stimulates mitochondrial ATP synthesis in resting muscle (Vrest). Recently, short-term high-intensity interval training (HIT) training has shown potent effects on Vmax . However, little is known about (1) the effects of short-term HIT on Vrest, and (2) the time course of adaptations in V rest and Vmax following short-term HIT. Healthy young males were recruited to participate in a 2-week training intervention (6 training sessions). Each session consisted of 4-6 bouts of 30-s sprints on a cycle ergometer. Phosphorus magnetic resonance spectroscopy was used to measure Vrest and Vmax in vivo in vastus lateralis: (i) prior to, (ii) 15 hours after a single session of exercise training, and (iii) after completing 6 sessions of exercise training. Two weeks of training increased peak whole-body oxygen consumption (35.8 ± 1.4 to 39.3 ± 1.6 ml·min-1 ·kg-1, p=0.01) and exercise capacity (217.0 ± 11.0 to 230.5 ± 11.7 W, p=0.04) on the cycle ergometer. While Vmax was unchanged after a single session of HIT, completion of six training sessions resulted in increased muscle oxidative capacity (p
Book Synopsis The Effects of High-intensity Interval Training on Skeletal Muscle Oxidative Capacity in Middle-aged Sedentary Adults by : Melanie Gardner
Download or read book The Effects of High-intensity Interval Training on Skeletal Muscle Oxidative Capacity in Middle-aged Sedentary Adults written by Melanie Gardner and published by . This book was released on 2009 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Science and Application of High-Intensity Interval Training by : Laursen, Paul
Download or read book Science and Application of High-Intensity Interval Training written by Laursen, Paul and published by Human Kinetics. This book was released on 2019 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The popularity of high-intensity interval training (HIIT), which consists primarily of repeated bursts of high-intensity exercise, continues to soar because its effectiveness and efficiency have been proven in use by both elite athletes and general fitness enthusiasts. Surprisingly, few resources have attempted to explain both the science behind the HIIT movement and its sport-specific application to athlete training. That’s why Science and Application of High-Intensity Interval Training is a must-have resource for sport coaches, strength and conditioning professionals, personal trainers, and exercise physiologists, as well as for researchers and sport scientists who study high-intensity interval training.
Book Synopsis High-Intensity Exercise in Hypoxia - Beneficial Aspects and Potential Drawbacks by : Olivier Girard
Download or read book High-Intensity Exercise in Hypoxia - Beneficial Aspects and Potential Drawbacks written by Olivier Girard and published by Frontiers Media SA. This book was released on 2018-01-25 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past, ‘traditional’ moderate-intensity continuous training (60-75% peak heart rate) was the type of physical activity most frequently recommended for both athletes and clinical populations (cf. American College of Sports Medicine guidelines). However, growing evidence indicates that high-intensity interval training (80-100% peak heart rate) could actually be associated with larger cardiorespiratory fitness and metabolic function benefits and, thereby, physical performance gains for athletes. Similarly, recent data in obese and hypertensive individuals indicate that various mechanisms – further improvement in endothelial function, reductions in sympathetic neural activity, or in arterial stiffness – might be involved in the larger cardiovascular protective effects associated with training at high exercise intensities. Concerning hypoxic training, similar trends have been observed from ‘traditional’ prolonged altitude sojourns (‘Live High Train High’ or ‘Live High Train Low’), which result in increased hemoglobin mass and blood carrying capacity. Recent innovative ‘Live Low Train High’ methods (‘Resistance Training in Hypoxia’ or ‘Repeated Sprint Training in Hypoxia’) have resulted in peripheral adaptations, such as hypertrophy or delay in muscle fatigue. Other interventions inducing peripheral hypoxia, such as vascular occlusion during endurance/resistance training or remote ischemic preconditioning (i.e. succession of ischemia/reperfusion episodes), have been proposed as methods for improving subsequent exercise performance or altitude tolerance (e.g. reduced severity of acute-mountain sickness symptoms). Postulated mechanisms behind these metabolic, neuro-humoral, hemodynamics, and systemic adaptations include stimulation of nitric oxide synthase, increase in anti-oxidant enzymes, and down-regulation of pro-inflammatory cytokines, although the amount of evidence is not yet significant enough. Improved O2 delivery/utilization conferred by hypoxic training interventions might also be effective in preventing and treating cardiovascular diseases, as well as contributing to improve exercise tolerance and health status of patients. For example, in obese subjects, combining exercise with hypoxic exposure enhances the negative energy balance, which further reduces weight and improves cardio-metabolic health. In hypertensive patients, the larger lowering of blood pressure through the endothelial nitric oxide synthase pathway and the associated compensatory vasodilation is taken to reflect the superiority of exercising in hypoxia compared to normoxia. A hypoxic stimulus, in addition to exercise at high vs. moderate intensity, has the potential to further ameliorate various aspects of the vascular function, as observed in healthy populations. This may have clinical implications for the reduction of cardiovascular risks. Key open questions are therefore of interest for patients suffering from chronic vascular or cellular hypoxia (e.g. work-rest or ischemia/reperfusion intermittent pattern; exercise intensity; hypoxic severity and exposure duration; type of hypoxia (normobaric vs. hypobaric); health risks; magnitude and maintenance of the benefits). Outside any potential beneficial effects of exercising in O2-deprived environments, there may also be long-term adverse consequences of chronic intermittent severe hypoxia. Sleep apnea syndrome, for instance, leads to oxidative stress and the production of reactive oxygen species, and ultimately systemic inflammation. Postulated pathophysiological changes associated with intermittent hypoxic exposure include alteration in baroreflex activity, increase in pulmonary arterial pressure and hematocrit, changes in heart structure and function, and an alteration in endothelial-dependent vasodilation in cerebral and muscular arteries. There is a need to explore the combination of exercising in hypoxia and association of hypertension, developmental defects, neuro-pathological and neuro-cognitive deficits, enhanced susceptibility to oxidative injury, and possibly increased myocardial and cerebral infarction in individuals sensitive to hypoxic stress. The aim of this Research Topic is to shed more light on the transcriptional, vascular, hemodynamics, neuro-humoral, and systemic consequences of training at high intensities under various hypoxic conditions.
Book Synopsis The Effects of High Intensity Interval Training on Skeletal Muscle Mitochondrial Dynamics in Diet-induced Obese Mice by : James B. Tincknell
Download or read book The Effects of High Intensity Interval Training on Skeletal Muscle Mitochondrial Dynamics in Diet-induced Obese Mice written by James B. Tincknell and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biologic Revelation by : Wayne Caparas
Download or read book Biologic Revelation written by Wayne Caparas and published by WestBow Press. This book was released on 2017-04-21 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expert Reviews: “Superbly researched, very convincing. . . BioLogic Revelation is a New Dawn in the way we need to work out. Forget your daily, hour long, sweaty, modestly effective exercise routine. You need quality, over quantity. There is a better, quicker, more effective way for better results based on solid scientific research. Personally, I modified my routine based on these well-researched facts and I am loving the results. A MUST READ!” Dr. Adrienne Denese, M.D. New York Times Bestselling Author Harvard Researcher, Anti-Aging Expert “If you want to burn fat, build lean muscle, look younger and maintain youthful joints then I highly recommend Wayne’s new book BioLogic Revelation. In this book Wayne unlocks the secrets to not only getting in the best shape of your life but also how to use fitness to help you slow the aging process. If you’re ready to transform your health and fitness level with less effort then I highly recommend reading this book and implementing the cutting edge and science-backed workout routine Wayne has created.” Dr. Josh Axe, Bestselling Author Natural Health & Fitness Expert Founder, DrAxe.com “BioLogic Revelation is going to revolutionize the fitness industry with its heavily researched, time-proven body of work. . . As a proponent of non-punishing fitness, I am relieved Wayne Caparas has written BioLogic Revelation. Now I have the resource to prove that more is not better, harder is not the answer, and proper form is always key to the results one needs and wants. Thanks to this groundbreaking book, undeniable proof is finally in black and white for all to see. Talk about a revelation!” Suzanne Bowen, Creator of BarreAmped Internationally Renowned Fitness Expert Founder, SuzanneBowenFitness.com “Cutting-edge yet easy to read and understand, Biologic Revelation is a truly original work, rich with innovative exercise techniques, exhaustive research, nutrition advice, and motivational strategies. . . Reading this book and following the program will change your life!” Dr. William Maguire, Jr., M.D. Two-Time Post and Courier Golden Pen Winner “I’ve been using variations of BioLogic Workouts for nearly a decade and the results have been phenomenal. So much so, that I started teaching the protocols of the BioLogic Method to my patients six years ago and all who stay the course enjoy the full cascade of benefits detailed in BioLogic Revelation.” Dr. Ben M. Sweeney Author, Natural Health & Healing Expert “Wayne is offering something new and fresh. He will challenge established fitness philosophy and back it up with solid scientific research. BioLogic Revelation has the potential to change the fitness landscape.” Dr. Kevin R. Baird, D. Min. Founder/Executive Director The Bonhoeffer Institute “BioLogic Revelation is a great work in every way. . . Wayne’s writing style makes this information-rich text exceptionally colorful and enjoyable to read, so you should have no problem making sense of all the science and putting it into action.” Scott Hasenbalg, Family Advocate President of Redemptive Ventures, LLC Founding Executive Director of Show Hope “I know those in the fitness field will have difficulty upsetting their historic “apple carts.” But if they can be open to new discoveries and the secrets revealed in BioLogic Revelation, many lives will be saved, and the joy for life will be multiplicatively enhanced.” Dr. Michael A. Kollar, Ed.D. Outstanding Lifetime Contribution To Psychology Award from the South Carolina Psychological Association
Book Synopsis Sports Nutrition & Performance Enhancing Supplements by : Abbie E. Smith-Ryan, Ph.D.
Download or read book Sports Nutrition & Performance Enhancing Supplements written by Abbie E. Smith-Ryan, Ph.D. and published by Linus Learning. This book was released on 2009 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Which of the following will yield the fastest measurable change in performance and/or body composition? (A) changes in training. (B) changes in diet. (C) use of sports supplements. (D) they all work equally well. If you answered 'C,' go to the head of the class. That's right; the proper use of sports supplements can produce changes in minutes (e.g. caffeine), days (e.g. creatine) and weeks (e.g. beta-alanine). Yet we are bombarded by muddled thinking from the mainstream media telling us that 'creatine causes cramps,' 'high protein diets are bad for your kidneys,' 'supplements aren't needed as long as you eat a balanced diet' and other ideas that are void of data. Sports Nutrition & Performance Enhancing Supplements (eds. Abbie Smith-Ryan PhD CSCS*D CISSN and Jose Antonio PhD FNSCA FISSN. Linus Publications) is a focused resource that will give you the latest sports nutrition science, and eradicate the intellectually lazy positions held so dearly by the anti-supplement crowd."--Amazon.
Book Synopsis The Pathway for Oxygen by : Ewald R. Weibel
Download or read book The Pathway for Oxygen written by Ewald R. Weibel and published by Harvard University Press. This book was released on 1984 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is rare indeed for one book to be both a first-rate classroom text and a major contribution to scholarship. The Pathway for Oxygen is such a book, offering a new approach to respiratory physiology and morphology that quantitatively links the two. Professionalism in science has led to a compartmentalization of biology. Function is the domain of the physiologist, structure that of the morphologist, and they often operate with vastly disparate concepts and procedures. Yet the performance of the respiratory system depends both on structural and on functional properties that cannot be separated. The first chapter of The Pathway for Oxygen engages the student with the design and function of the vertebrate respiratory organs from a comparative viewpoint. The second chapter adds to that foundation the link between cell energetics and oxygen needs of the whole animal. With Chapter 3 the excitement begins--new ideas, fresh attacks on old problems, and a fuller account of the power of the quantitative approach Dr. Weibel has pioneered. The Pathway for Oxygen will be read eagerly by medical students, graduate students, advanced undergraduates in zoology--and by their professors.
Author :Luc J. C. van Loon Publisher :Karger Medical and Scientific Publishers ISBN 13 :3318024082 Total Pages :146 pages Book Rating :4.3/5 (18 download)
Book Synopsis Limits of Human Endurance by : Luc J. C. van Loon
Download or read book Limits of Human Endurance written by Luc J. C. van Loon and published by Karger Medical and Scientific Publishers. This book was released on 2013 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to improve exercise performance capacity through adequate nutrition Nutrition is one of the key factors that modulate exercise performance. In this book, a group of expert scientists discuss the ergogenic properties of various nutritional interventions and present research to show that dietary strategies can be applied to extend the limits of human endurance, lower the risk of illness or injury, and speed recovery rates. More specifically, they discuss recent findings on topics such as caffeine and its effect on the brain, carnitine and fat oxidation, ergogenic properties of beta- alanine, dietary protein and muscle reconditioning, nutrition and immune status, and the importance of proper hydration. This publication will provide the reader with many novel insights into the complex interaction between nutrition and exercise, allowing them to define more effective dietary strategies to improve health and performance. Moreover, while focusing on elite athletes, it is interesting to note that some of the discoveries can be applied beyond this niche, for example to improve performance outcomes in the elderly.
Book Synopsis Science and Development of Muscle Hypertrophy by : Brad J. Schoenfeld
Download or read book Science and Development of Muscle Hypertrophy written by Brad J. Schoenfeld and published by Human Kinetics. This book was released on 2020-05-08 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Science and Development of Muscle Hypertrophy, Second Edition, is the most comprehensive resource on muscle hypertrophy in the world. Written by Brad Schoenfeld, PhD, an internationally renowned expert on muscle hypertrophy, this book is the definitive resource for strength and conditioning professionals, personal trainers, sport scientists, researchers, and exercise science instructors who are seeking information regarding muscle hypertrophy, including the mechanism of its development, how the body structurally and hormonally changes when exposed to stress, ways to most effectively design training programs, and nutritional guidelines for eliciting hypertrophic changes. This new edition offers more than 1,000 references and applied guidelines. Two all-new chapters deliver practical content on the measurement of muscle hypertrophy and advanced training practices. Readers will learn various methods by which hypertrophy is measured, including site-specific measures (circumference measures, MRI, CT, and ultrasound), indirect measures (underwater weighing, DXA, BIA, ADP, and skinfolds), and histological measures (biopsy), as well as the strengths and limitations of each modality. The new edition also provides guidance for achieving greater training volumes with training practices that maximize the individual’s genetic potential to gain muscle. No other resource offers a comparable amount of content solely focused on the science of muscle hypertrophy and its application to designing training programs. The full-color book offers several features to make the content accessible to readers: Research Findings sidebars highlight the aspects of muscle hypertrophy currently being examined to encourage readers to re-evaluate their knowledge and ensure their training practices are up to date. Practical Applications sidebars outline how to apply the research conclusions for maximal hypertrophic development. Comprehensive subject and author indexes optimize the book’s use as a reference tool. Although muscle hypertrophy can be attained through a range of training programs, this book allows readers to understand and apply the specific responses and mechanisms that promote optimal muscle hypertrophy. It explores how genetic background, age, sex, and other factors have been shown to mediate the hypertrophic response to exercise, affecting both the rate and the total gain in lean muscle mass. Sample programs show how to design a three- or four-day-per-week undulating periodized program and a modified linear periodized program for maximizing muscular development. Science and Development of Muscle Hypertrophy is an invaluable resource for those seeking to maximize hypertrophic gains for themselves or their athletes or clients and for those searching for the most comprehensive and authoritative research in the field. Earn continuing education credits/units! A continuing education course and exam that uses this book is also available. It may be purchased separately or as part of a package that includes all the course materials and exam.
Book Synopsis Mitochondria in Obesity and Type 2 Diabetes by : Beatrice Morio
Download or read book Mitochondria in Obesity and Type 2 Diabetes written by Beatrice Morio and published by Academic Press. This book was released on 2019-04-26 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondria in Obesity and Type 2 Diabetes: Comprehensive Review on Mitochondrial Functioning and Involvement in Metabolic Diseases synthesizes discoveries from laboratories around the world, enhancing our understanding of the involvement of mitochondria in the etiology of diseases, such as obesity and type 2 diabetes. Chapters illustrate and provide an overview of key concepts on topics such as the role of mitochondria in adipose tissue, cancer, cardiovascular comorbidities, skeletal muscle, the liver, kidney, and more. This book is a must-have reference for students and educational teams in biology, physiology and medicine, and researchers. Synthesizes actual knowledge on mitochondrial function Provides an integrated vision of each tissue in the etiology of obesity and type 2 diabetes Identifies the interactive networks that involve alteration in mitochondrial mass and function in disease progression Highlights the role played by mitochondria in the prevention and treatment of obesity and type 2 diabetes
Book Synopsis The Impact of Interval Intensity in Overweight Young Men by : John Colin Boyd
Download or read book The Impact of Interval Intensity in Overweight Young Men written by John Colin Boyd and published by . This book was released on 2012 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: Considering the increasing global prevalence of overweight and obesity and their propensity for disease, this study was undertaken in an attempt to optimize exercise prescription for this at-risk group by determining if the benefits associated with interval training occur in an intensity dependent manner. 19 sedentary, overweight males (Age: 22.7 ± 3.9 yrs, BMI: 31.4 ± 2.6 kg/m2, WC: 106.5 ± 6.6 cm) performed interval training for three weeks at either 70% or 100% of their peak work rate on a cycle ergometer. Aerobic capacity measurements, time to completion trials, muscle biopsies, and fasted blood samples were all performed pre and post training. Analyses of aerobic capacity and exercise performance demonstrate greater improvements made in the 100% compared to the 70% group, while measures of skeletal muscle oxidative capacity indicate equivalent changes between groups. Taking into account the similar increases in mitochondrial content in both groups and understanding the influence of both oxygen supply and demand in determining maximal oxygen consumption, the greater increases in aerobic capacity achieved by the 100% group may be the result of enhanced cardiovascular adaptations. These findings suggest that some of the health benefits associated with interval exercise may be intensity dependent. Therefore, there may be additional benefit to exercise at higher intensities.
Book Synopsis Exercise and Diabetes by : Sheri R. Colberg
Download or read book Exercise and Diabetes written by Sheri R. Colberg and published by American Diabetes Association. This book was released on 2013-05-30 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical movement has a positive effect on physical fitness, morbidity, and mortality in individuals with diabetes. Although exercise has long been considered a cornerstone of diabetes management, many health care providers fail to prescribe it. In addition, many fitness professionals may be unaware of the complexities of including physical activity in the management of diabetes. Giving patients or clients a full exercise prescription that take other chronic conditions commonly accompanying diabetes into account may be too time-consuming for or beyond the expertise of many health care and fitness professionals. The purpose of this book is to cover the recommended types and quantities of physical activities that can and should be undertaken by all individuals with any type of diabetes, along with precautions related to medication use and diabetes-related health complications. Medications used to control diabetes should augment lifestyle improvements like increased daily physical activity rather than replace them. Up until now, professional books with exercise information and prescriptions were not timely or interactive enough to easily provide busy professionals with access to the latest recommendations for each unique patient. However, simply instructing patients to “exercise more” is frequently not motivating or informative enough to get them regularly or safely active. This book is changing all that with its up-to-date and easy-to-prescribe exercise and physical activity recommendations and relevant case studies. Read and learn to quickly prescribe effective and appropriate exercise to everyone.
Book Synopsis Science and Application of High-Intensity Interval Training by : Paul Laursen
Download or read book Science and Application of High-Intensity Interval Training written by Paul Laursen and published by Human Kinetics. This book was released on 2019-01-02 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: The popularity of high-intensity interval training (HIIT), which consists primarily of repeated bursts of high-intensity exercise, continues to soar because its effectiveness and efficiency have been proven in use by both elite athletes and general fitness enthusiasts. Surprisingly, few resources have attempted to explain both the science behind the HIIT movement and its sport-specific application to athlete training. That’s why Science and Application of High-Intensity Interval Training is a must-have resource for sport coaches, strength and conditioning professionals, personal trainers, and exercise physiologists, as well as for researchers and sport scientists who study high-intensity interval training. Authors Paul Laursen and Martin Buchheit—both well-known, expert-level HIIT researchers as well as practitioners and endurance athletes—do a masterful job of blending science-based concepts of HIIT with practical application strategies. Laursen, Buchheit, and a team of highly qualified contributors—who bring hundreds of years of combined HIIT science and application experience from across all sports—have written Science and Application of High-Intensity Interval Training to provide practitioners and athletes an understanding of the foundational principles of HIIT programming. Chapters in the first section describe five types of training, how to manipulate HIIT variables to maximize improvements in physical performance, and how to incorporate HIIT into a general training program. Readers will also learn the influence HIIT can have on fatigue, stress, and an athlete’s overall health. The final 20 chapters each focus on a different sport and are written by leading coaches or practitioners who have successfully applied HIIT principles at an elite level in their respective sport. These chapters describe specific ways to incorporate HIIT into a training regimen for everything from combat sports to endurance events to the most popular U.S. and international individual and team sports. Each chapter also contains sport-specific preparation and competition phases, an overall one-year training program, and a brief story of how the coach or practitioner who authored the chapter used HIIT to successfully prepare an athlete for a competition. Knowing the proper ways to incorporate high-intensity interval training into a fitness or conditioning program is of vital importance: Not following proper protocols can lead to excessive and prolonged fatigue, illness, or injury. Science and Application of High-Intensity Interval Training is an essential guide for those who want to incorporate HIIT into their own training or their athletes’ programming. Earn continuing education credits/units! A continuing education course and exam that uses this book is also available. It may be purchased separately or as part of a package that includes all the course materials and exam.
Book Synopsis Effects of Different Training Intensities in High-intensity Interval Training (HIIT) on Maximal Aerobic Velocity, Hematological and Muscle-damage Markers in Healthy Young Adults by : Fatma Rhibi
Download or read book Effects of Different Training Intensities in High-intensity Interval Training (HIIT) on Maximal Aerobic Velocity, Hematological and Muscle-damage Markers in Healthy Young Adults written by Fatma Rhibi and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Obesity and Lipotoxicity by : Ayse Basak Engin
Download or read book Obesity and Lipotoxicity written by Ayse Basak Engin and published by Springer. This book was released on 2017-06-05 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the resultant health consequences and considerable increase in prevalence, obesity has become a major worldwide health problem. “Obesity and Lipotoxicity” is a comprehensive review of the recent researches to provide a better understanding of the lipotoxicity-related mechanisms of obesity and the potential for the development of new treatment strategies. This book overviews the biochemical pathways leading to obesity-related metabolic disorders that occur subsequent to lipotoxicity. Chapters examine the deleterious effects of nutrient excess at molecular level including the cellular and molecular aspects of breast cancer, resistance to leptin, insulin, adiponectin, and interconnection between the circadian clock and metabolic pathways during high-fat feeding. “Lipotoxicity and Obesity” will be a useful resource for clinicians and basic science researchers, such as biochemists, toxicologists, immunologists, nutritionists, adult and pediatric endocrinologists, cardiologists, as well as students who are thought in this field.
Book Synopsis Enzymatic Analysis by : Janet V. Passonneau
Download or read book Enzymatic Analysis written by Janet V. Passonneau and published by Springer Science & Business Media. This book was released on 2008-02-07 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
