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Book Synopsis Dynamics of Estrogen Receptor Transcription Complex Assembly in Breast Cancer by :
Download or read book Dynamics of Estrogen Receptor Transcription Complex Assembly in Breast Cancer written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Estrogen plays a critical role in the development and progression of breast cancer. While endocrine therapies play an important part in breast cancer treatment, the failure of these therapies reflects a lack of knowledge concerning the molecular mechanisms involved in estrogen signaling. The biological activities of estrogen are mediated by estrogen receptors (ER) . In addition, a large number of proteins termed cofactors are involved in ER signaling. Until recently, our knowledge regarding these cofactors was based on their ability to bind receptors in vitro and affect transcriptional activation in transfection experiments. The in vivo role of these cofactors and the specific target genes involved in breast cancer are not well known. Therapeutic agents, such as tamoxifen, also bind ER, but block proliferation in breast cells. However, tamoxifen increases the risk of endometrial cancer. We have used chromatin immunoprecipitation (ChIP) to investigate cofactor involvement in ER signaling in vivo and to understand the mechanisms underlying the different actions of tamoxifen in breast and endometrial cells. We are in the process of using ChIP to identify the set of genes regulated by ER and its cofactors in these tissues. The detailed understanding of tissue- and ligand-dependent changes in gene expression gained through these studies will lead to more effective therapies for ER-dependent breast cancer.