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Author : Boehringer Mannheim GmbH Biochemica
Publisher :
ISBN 13 :
Total Pages : 62 pages
Book Rating : 4.:/5 (397 download)
Download or read book DNA Labeling and Detection Nonradioactive written by Boehringer Mannheim GmbH Biochemica and published by . This book was released on 1989 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Christoph Kessler
Publisher : Springer
ISBN 13 : 3662001446
Total Pages : 449 pages
Book Rating : 4.6/5 (62 download)
Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 2012-12-06 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author : Christoph Kessler
Publisher : Springer Science & Business Media
ISBN 13 : 9783540646013
Total Pages : 1170 pages
Book Rating : 4.6/5 (46 download)
Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2000-03-11 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Author : A. J. Garman
Publisher : Elsevier
ISBN 13 : 0080551092
Total Pages : 155 pages
Book Rating : 4.0/5 (85 download)
Download or read book Non-Radioactive Labelling written by A. J. Garman and published by Elsevier. This book was released on 1997-03-20 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. Aimed at researchers without specific expertise in chemistry, the book includes: A review of the main signal systems and labels available, indicating their strengths and weaknesses Discussion of the most useful strategies for labelling the various biomolecules 32 protocols covering common labelling needs Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications Sources of information including references, data and suppliers
Author : Larry J. Kricka
Publisher : Academic Press
ISBN 13 : 0323137717
Total Pages : 373 pages
Book Rating : 4.3/5 (231 download)
Download or read book Nonisotopic DNA Probe Techniques written by Larry J. Kricka and published by Academic Press. This book was released on 2012-12-02 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided. This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection. Gives extensive practical information Covers major types of nonisotopic labels and procedures Presents background information for each method Provides strategies and detailed experimental procedures for labeling and detecting DNA sequences by Fluorescence Chemiluminescence Bioluminescence Colorimetry
Author : Giuseppe Spoto
Publisher : Springer Science & Business Media
ISBN 13 : 940071226X
Total Pages : 320 pages
Book Rating : 4.4/5 (7 download)
Download or read book Detection of Non-Amplified Genomic DNA written by Giuseppe Spoto and published by Springer Science & Business Media. This book was released on 2012-07-06 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.
Author : Elena Hilario
Publisher : Springer Science & Business Media
ISBN 13 : 1597452297
Total Pages : 318 pages
Book Rating : 4.5/5 (974 download)
Download or read book Protocols for Nucleic Acid Analysis by Nonradioactive Probes written by Elena Hilario and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author : Christoph Kessler
Publisher : Springer Verlag
ISBN 13 : 9780387554822
Total Pages : 436 pages
Book Rating : 4.5/5 (548 download)
Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer Verlag. This book was released on 1992-01-01 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : T. Takahashi
Publisher :
ISBN 13 :
Total Pages : 7 pages
Book Rating : 4.:/5 ( download)
Download or read book A Nonradioactive Nucleic Acid Labeling Method and a Detection Method Using it written by T. Takahashi and published by . This book was released on 1989 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Yen-Ping Kuo
Publisher :
ISBN 13 :
Total Pages : 216 pages
Book Rating : 4.3/5 (129 download)
Download or read book Detection of Enterohemorrhagic Escherichia Coli 0157:H7 by Radioactive and Nonradioactive DNA Probes written by Yen-Ping Kuo and published by . This book was released on 1989 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : George H. Keller
Publisher :
ISBN 13 :
Total Pages : 288 pages
Book Rating : 4.3/5 (91 download)
Download or read book DNA Probes written by George H. Keller and published by . This book was released on 1989 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text concentrates on DNA probes, providing background information, sample preparation, isotopic labeling procedures, non-isotopic labeling procedures, hybridization formats and detection procedures, and amplification systems.
Author : Yeuk-Nam Kennie Tang
Publisher : Open Dissertation Press
ISBN 13 : 9781360978505
Total Pages : 110 pages
Book Rating : 4.9/5 (785 download)
Download or read book COMPARISON OF DIG NONRADIOACTI written by Yeuk-Nam Kennie Tang and published by Open Dissertation Press. This book was released on 2017-01-26 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "A Comparison of DIG Nonradioactive With 32p Radioactive Nucleic Acid Labeling of Southern Blot for the Detection of Alpha Thalassaemia" by Yeuk-nam, Kennie, Tang, 鄧若楠, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b4501045 Subjects: Thalassemia - Diagnosis DNA - Analysis
Author : Yeuk-nam Tang (Kennie)
Publisher :
ISBN 13 :
Total Pages : 186 pages
Book Rating : 4.:/5 (71 download)
Download or read book A Comparison of DIG Nonradioactive with 32p Radioactive Nucleic Acid Labeling of Southern Blot for the Detection of Alpha Thalassaemia written by Yeuk-nam Tang (Kennie) and published by . This book was released on 2005 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Stefan Surzycki
Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.6/5 (425 download)
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author : Gary C. Howard
Publisher : McGraw-Hill/Appleton & Lange
ISBN 13 : 9780444016614
Total Pages : 342 pages
Book Rating : 4.0/5 (166 download)
Download or read book Methods in Nonradioactive Detection written by Gary C. Howard and published by McGraw-Hill/Appleton & Lange. This book was released on 1993 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein methods. Biotin labeling methods for proteins and carbohydrates. Direct conjugation of enzymes to proteins. Labeling proteins with fluorochromes. Comparing fluorescent organic dyes for biomolecular labeling. Chemiluminescent detection using alkaline phosphatase-the chemistry. Detection of proteins on filters by enzymatic methods. Enzyme-linked immunosorbent assays. Automation of protein and immunochemistry methods. DNA methods. Biotin labeling methods for DNA. Direct attachment of enzymes to DNA probes. Detection of nucleic acids on filters by enzymatic methods. Polymerase chain reaction and its impact on DNA detection limits. Automated procedures for DNA methods. Tissue and cell methods. Enzyme-based methods in tissue sections. In situ hybridization. Chromosome mapping by fluorescent in situ hybridization. Colloidal gold localization of in situ hybrids. Immunofluorescence methods for microscopic analysis. Flow cytometry methods.
Author : P. Lebacq
Publisher :
ISBN 13 :
Total Pages : 18 pages
Book Rating : 4.:/5 ( download)
Download or read book Process for Staining of Nucleic Acids Detected by Non-radioactive Labeling and Reagent Kit for the Use of this Process written by P. Lebacq and published by . This book was released on 1989 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : John M. Walker
Publisher : Springer Science & Business Media
ISBN 13 : 1603273751
Total Pages : 1105 pages
Book Rating : 4.6/5 (32 download)
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
