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Dna Cloning A Hands On Approach
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Book Synopsis DNA Cloning: A Hands-on Approach by : Seok-Yong Choi
Download or read book DNA Cloning: A Hands-on Approach written by Seok-Yong Choi and published by Springer. This book was released on 2019-04-17 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Book Synopsis Molecular Biology Techniques by : Heather B. Miller
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book DNA Cloning written by Seok-Yong Choi and published by . This book was released on 2019 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Book Synopsis Safety of Genetically Engineered Foods by : National Research Council
Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Book Synopsis Laboratory Methods in Enzymology: DNA by :
Download or read book Laboratory Methods in Enzymology: DNA written by and published by Elsevier. This book was released on 2013-09-02 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on DNA
Book Synopsis Methods in Molecular Biology and Protein Chemistry by : Brenda D. Spangler
Download or read book Methods in Molecular Biology and Protein Chemistry written by Brenda D. Spangler and published by Wiley. This book was released on 2002-06-14 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ein praxisorientierter Leitfaden für Studenten im Hauptstudium. Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes. Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen. Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung. Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten. Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien. Verständlich und anschaulich geschrieben. Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden. "Methods in Molecular Biology" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren.
Book Synopsis New Directions for Biosciences Research in Agriculture by : National Research Council
Download or read book New Directions for Biosciences Research in Agriculture written by National Research Council and published by National Academies Press. This book was released on 1985-01-01 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research. Directed to the Agricultural Research Service and the agricultural research community at large, the volume discusses biosciences research in genetic engineering, animal science, plant science, and plant diseases and insect pests. An optimal climate for productive research is discussed.
Author :Committee on Technological Innovation in Medicine Publisher :National Academies Press ISBN 13 :0309587611 Total Pages :255 pages Book Rating :4.3/5 (95 download)
Book Synopsis Sources of Medical Technology by : Committee on Technological Innovation in Medicine
Download or read book Sources of Medical Technology written by Committee on Technological Innovation in Medicine and published by National Academies Press. This book was released on 1995-01-15 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Evidence suggests that medical innovation is becoming increasingly dependent on interdisciplinary research and on the crossing of institutional boundaries. This volume focuses on the conditions governing the supply of new medical technologies and suggest that the boundaries between disciplines, institutions, and the private and public sectors have been redrawn and reshaped. Individual essays explore the nature, organization, and management of interdisciplinary R&D in medicine; the introduction into clinical practice of the laser, endoscopic innovations, cochlear implantation, cardiovascular imaging technologies, and synthetic insulin; the division of innovating labor in biotechnology; the government- industry-university interface; perspectives on industrial R&D management; and the growing intertwining of the public and proprietary in medical technology.
Book Synopsis Gene Cloning and Manipulation by : Christopher Howe
Download or read book Gene Cloning and Manipulation written by Christopher Howe and published by Cambridge University Press. This book was released on 2007-07-12 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Book Synopsis Mapping and Sequencing the Human Genome by : National Research Council
Download or read book Mapping and Sequencing the Human Genome written by National Research Council and published by National Academies Press. This book was released on 1988-01-01 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.
Download or read book Gene Biotechnology written by William Wu and published by CRC Press. This book was released on 2016-04-19 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the princ
Book Synopsis Molecular Cloning by : Joseph Sambrook
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis How to Clone a Mammoth by : Beth Shapiro
Download or read book How to Clone a Mammoth written by Beth Shapiro and published by Princeton University Press. This book was released on 2020-09-08 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: An insider's view on bringing extinct species back to life Could extinct species, like mammoths and passenger pigeons, be brought back to life? In How to Clone a Mammoth, Beth Shapiro, an evolutionary biologist and pioneer in ancient DNA research, addresses this intriguing question by walking readers through the astonishing and controversial process of de-extinction. From deciding which species should be restored to anticipating how revived populations might be overseen in the wild, Shapiro vividly explores the extraordinary cutting-edge science that is being used to resurrect the past. Considering de-extinction's practical benefits and ethical challenges, Shapiro argues that the overarching goal should be the revitalization and stabilization of contemporary ecosystems. Looking at the very real and compelling science behind an idea once seen as science fiction, How to Clone a Mammoth demonstrates how de-extinction will redefine conservation's future.
Book Synopsis Introduction to Pharmaceutical Biotechnology, Volume 1 (Second Edition): Basic Techniques and Concepts by :
Download or read book Introduction to Pharmaceutical Biotechnology, Volume 1 (Second Edition): Basic Techniques and Concepts written by and published by . This book was released on 2024-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Stem Cells: An Insider's Guide by : Paul Knoepfler
Download or read book Stem Cells: An Insider's Guide written by Paul Knoepfler and published by World Scientific. This book was released on 2013-07-30 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem Cells: An Insider's Guide is an exciting new book that takes readers inside the world of stem cells guided by international stem cell expert, Dr. Paul Knoepfler. Stem cells are catalyzing a revolution in medicine. The book also tackles the exciting and hotly debated area of stem cell treatments that are capturing the public's imagination. In the future they may also transform how we age and reproduce. However, there are serious risks and ethical challenges, too. The author's goal with this insider's guide is to give readers the information needed to distinguish between the ubiquitous hype and legitimate hope found throughout the stem cell world. The book answers the most common questions that people have about stem cells. Can stem cells help my family with a serious medical problem such as Alzheimer's, Multiple Sclerosis, or Autism? Are such treatments safe? Can stem cells make me look younger or even literally stay physically young? These questions and many more are answered here.A number of ethical issues related to stem cells that spark debates are discussed, including risky treatments, cloning and embryonic stem cells. The author breaks new ground in a number of ways such as by suggesting reforms to the FDA, providing a new theory of aging based on stem cells, and including a revolutionary Stem Cell Patient Bill of Rights. More generally, the book is your guide to where the stem cell field will be in the near future as well as a thoughtful perspective on how stem cell therapies will ultimately change your life and our world.
Book Synopsis Gene Cloning and DNA Analysis by : T. A. Brown
Download or read book Gene Cloning and DNA Analysis written by T. A. Brown and published by John Wiley & Sons. This book was released on 2013-04-25 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)
