Developmental Biology Protocols, Volumes I, II, and III

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Publisher : Humana Press
ISBN 13 : 9780896038554
Total Pages : 0 pages
Book Rating : 4.0/5 (385 download)

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Book Synopsis Developmental Biology Protocols, Volumes I, II, and III by : Rocky S. Tuan

Download or read book Developmental Biology Protocols, Volumes I, II, and III written by Rocky S. Tuan and published by Humana Press. This book was released on 1999-12-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fast-growing field of developmental biology provides a unifying framework that integrates anatomy, physiology, genetics, biochemistry, cellular and molecular biology, as well as evolutionary biology. The number of "reference" publications that deal specifically with the practical aspects of experimental developmental biology are relatively scarce. Developmental Biology Protocols grows out of this need for a comprehensive laboratory manual that provides the readers with the principles, background, rationale, as well as practical protocols, for studying and analyzing the events of embryonic development. Its highly practical format and wide range of model systems and multidisciplinary experimental techniques attest to the authors' determination to provide a balanced presentation of both background information and actual laboratory details. This three-volume compilation of relevant and useful information will be a well-utilized resource for both the students and teachers of developmental biology at all levels. Developmental Biology Protocols offers the most comprehensive, cutting-edge collection of contemporary experimental methods available for the study of embryogenesis and development. Written by an interdisciplinary team of leading scientific investigators, this authoritative collection provides step-by-step instructions for successful laboratory execution. Topics range from animal model system acquisition to molecular genetics, and include high resolution imaging, transgenesis, teratology, comparative anatomy and embryology, the characterization of embryonic structure and function, the analysis of cell function, and the regulation of gene activity. In addition to many unique, new experimental protocols, the authors have also utilized a host of experimental approaches used in other disciplines applicable to the study of development. Comprehensive and richly annotated, Developmental Biology Protocols constitutes the gold-standard reference for today's developmental biologists.

Developmental Biology Protocols

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1592590659
Total Pages : 537 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Developmental Biology Protocols by : Rocky S. Tuan

Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Developmental Biology Protocols

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1592590667
Total Pages : 530 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Developmental Biology Protocols by : Rocky S. Tuan

Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Developmental Biology Protocols

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Author :
Publisher : Humana Press
ISBN 13 : 9780896035751
Total Pages : 505 pages
Book Rating : 4.0/5 (357 download)

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Book Synopsis Developmental Biology Protocols by : Rocky S. Tuan

Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Humana Press. This book was released on 2000-01-17 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Developmental Biology Protocols

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Author :
Publisher : Humana Press
ISBN 13 : 9780896038523
Total Pages : 0 pages
Book Rating : 4.0/5 (385 download)

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Book Synopsis Developmental Biology Protocols by : Rocky S. Tuan

Download or read book Developmental Biology Protocols written by Rocky S. Tuan and published by Humana Press. This book was released on 1999-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences alike. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Current Protocols in Molecular Biology

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Publisher :
ISBN 13 : 9780471503385
Total Pages : pages
Book Rating : 4.5/5 (33 download)

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Book Synopsis Current Protocols in Molecular Biology by :

Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Embryonic Stem Cell Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1597450375
Total Pages : 504 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Embryonic Stem Cell Protocols by : Kursad Turksen

Download or read book Embryonic Stem Cell Protocols written by Kursad Turksen and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Avian and Reptilian Developmental Biology

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Publisher :
ISBN 13 : 9781493972166
Total Pages : 365 pages
Book Rating : 4.9/5 (721 download)

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Book Synopsis Avian and Reptilian Developmental Biology by : Guojun Sheng

Download or read book Avian and Reptilian Developmental Biology written by Guojun Sheng and published by . This book was released on 2017 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a variety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

Essential Zebrafish Methods: Cell and Developmental Biology

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Publisher : Academic Press
ISBN 13 : 0080923437
Total Pages : 581 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Essential Zebrafish Methods: Cell and Developmental Biology by : Monte Westerfield

Download or read book Essential Zebrafish Methods: Cell and Developmental Biology written by Monte Westerfield and published by Academic Press. This book was released on 2009-08-27 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade to both experienced researchers and more junior members in the lab. Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error

Protein Purification Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 159259655X
Total Pages : 474 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Protein Purification Protocols by : Paul Cutler

Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Craniofacial Development

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Publisher :
ISBN 13 : 9781071618479
Total Pages : 313 pages
Book Rating : 4.6/5 (184 download)

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Book Synopsis Craniofacial Development by : Sebastian Dworkin

Download or read book Craniofacial Development written by Sebastian Dworkin and published by . This book was released on 2022 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. .

The Zebrafish: Cellular and Developmental Biology

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Publisher : Academic Press
ISBN 13 : 9780123848932
Total Pages : 366 pages
Book Rating : 4.8/5 (489 download)

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Book Synopsis The Zebrafish: Cellular and Developmental Biology by :

Download or read book The Zebrafish: Cellular and Developmental Biology written by and published by Academic Press. This book was released on 2010-12-24 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls Summarizes the Zebrafish Genome Project

Molecular Embryology

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Publisher : Springer Science & Business Media
ISBN 13 : 1592592708
Total Pages : 752 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Molecular Embryology by : Paul T. Sharpe

Download or read book Molecular Embryology written by Paul T. Sharpe and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.

Plant Developmental Biology

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Publisher : Humana
ISBN 13 : 9781493957347
Total Pages : 448 pages
Book Rating : 4.9/5 (573 download)

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Book Synopsis Plant Developmental Biology by : Lars Hennig

Download or read book Plant Developmental Biology written by Lars Hennig and published by Humana. This book was released on 2016-08-23 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.

Molecular Methods in Developmental Biology

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Publisher : Springer Science & Business Media
ISBN 13 : 1592596789
Total Pages : 222 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Molecular Methods in Developmental Biology by : Matt Guille

Download or read book Molecular Methods in Developmental Biology written by Matt Guille and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.

Plant Developmental Biology

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Author :
Publisher : Humana Press
ISBN 13 : 9781607617662
Total Pages : 448 pages
Book Rating : 4.6/5 (176 download)

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Book Synopsis Plant Developmental Biology by : Lars Hennig

Download or read book Plant Developmental Biology written by Lars Hennig and published by Humana Press. This book was released on 2011-04-06 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.

PCR Protocols

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Publisher : Springer Science & Business Media
ISBN 13 : 1592593844
Total Pages : 1083 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis PCR Protocols by : John M. S. Bartlett

Download or read book PCR Protocols written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 1083 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.