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Development Of Novel Multi Dimensional Separation Methods For Analysis Of The Proteome Using Iso Electric Focusing Reversed Phase Hplc Maldi Tof Ms And Esi Tof Ms
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Book Synopsis Development of Novel Multi-dimensional Separation Methods for Analysis of the Proteome Using Iso-electric Focusing, Reversed Phase HPLC, MALDI TOF/MS and ESI TOF/MS. by : Daniel B. Wall
Download or read book Development of Novel Multi-dimensional Separation Methods for Analysis of the Proteome Using Iso-electric Focusing, Reversed Phase HPLC, MALDI TOF/MS and ESI TOF/MS. written by Daniel B. Wall and published by . This book was released on 2001 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Recent Developments in Applied Microbiology and Biochemistry by : Viswanath Buddolla
Download or read book Recent Developments in Applied Microbiology and Biochemistry written by Viswanath Buddolla and published by Academic Press. This book was released on 2020-10-15 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent Developments in Applied Microbiology and Biochemistry, Vol. 2, provides a comprehensive treatment and understanding on application oriented microbial concepts, giving readers insights into recent developments in microbial biotechnology and medical, agricultural and environmental microbiology. Discusses microbial proteome analyses and their importance in medical microbiology Explores emerging trends in the prevention of current global health problems, such as cancer, obesity and immunity Shows recent approaches in the production of novel enzymes from environmental samples by enrichment culture and metagenomics approaches Guides readers through the status and recent developments in analytical methods for the detection of foodborne microorganisms
Book Synopsis Novel Micro-separation Techniques and Their Couplings to Mass Spectrometry for the Analysis of Complex Peptides and Proteins by :
Download or read book Novel Micro-separation Techniques and Their Couplings to Mass Spectrometry for the Analysis of Complex Peptides and Proteins written by and published by . This book was released on 2013 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS)-based technology has evolved as a powerful tool for the analysis of a wide array of biomolecules. However, its application to complex peptidomics and proteomics samples, for example neuropeptides, has proven to be challenging due to their extreme chemical complexities and low abundances in biological tissue samples. This PhD dissertation is focused on addressing some of these challenges by developing novel micro-separation tools and coupling them with MS to achieve enhanced MS sensitivity and increased peptide and protein coverage with lower sample consumption and reduced experimental time. Both chromatography-based and electrophoresis-based separations as well as their couplings to MS have been explored in this work, including monolithic material-based chromatography, improved capillary electrophoresis (CE) separation and novel coupling of separations to mass spectrometric imaging. N-acryloxysuccinimide-based monolithic column has been fabricated with specific antibodies immobilized onto the column to enable the extraction of targeted neuropeptide family from biological matrices. Alternatively, enzymes were immobilized onto the column for highly efficient on-column enzymatic digestion for proteomics studies. A methacrylate-based monolithic column with C12 functional groups was developed and coupled to both electrospray ionization (ESI) MS and matrix-assisted laser desorption/ionization (MALDI) MS for reversed-phase liquid chromatography (RPLC). For capillary electrophoresis, two improved capillary isoelectric focusing (CIEF) systems have been developed, including membrane-assisted CIEF and immobilized pH-gradient CIEF to obtain reduced sample loss and enhanced MS signal for neuropeptide analysis. An improved sheathless pressure-assisted CE (PACE) system was also developed for coupling to MALDI MS. For novel separation-MS imaging coupling, three generations of interfaces have been designed to couple CE, LC and multi-dimensional separations to MS imaging (MSI) for quantitative analysis of complex peptides. Collectively, this work results in development of the new generation of micro-separation and sample preparation tools and their coupling to various mass spectrometric ionization and detection modes for sensitive and rapid analysis of complex peptides and proteins. The technological innovations have significantly expanded the toolbox for peptidomics/proteomics analysis and will enable more in-depth investigations on peptide function and regulation.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2001 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development of Multidimensional Liquid Phase Protein Separations for Proteomic Analysis by : Bobby Fachrizal Assiddiq
Download or read book Development of Multidimensional Liquid Phase Protein Separations for Proteomic Analysis written by Bobby Fachrizal Assiddiq and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Multidimensional Analyses of the Proteome Using Nonporous Reversed-phase HPLC Coupled with MALDI-TOF and ESI Mass Spectrometry by : Bathsheba E. Chong
Download or read book Multidimensional Analyses of the Proteome Using Nonporous Reversed-phase HPLC Coupled with MALDI-TOF and ESI Mass Spectrometry written by Bathsheba E. Chong and published by . This book was released on 2000 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 2000 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Separation Methods In Proteomics by : Gary B. Smejkal
Download or read book Separation Methods In Proteomics written by Gary B. Smejkal and published by CRC Press. This book was released on 2005-12-12 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomic
Book Synopsis Multidimensional Liquid Chromatographic Separations for Proteomics by : Karl Burgess
Download or read book Multidimensional Liquid Chromatographic Separations for Proteomics written by Karl Burgess and published by . This book was released on 2008 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sample complexity is one of the key challenges facing contemporary proteomic analysis. A variety of methods is commonly employed to reduce this complexity, both at an intact protein and digested peptide level. For complex lysates containing many thousands of proteins, orthogonal (mutually independent), multidimensional separation methods must be employed to provide sufficient resolution to characterize the appropriate number of different species. The most common of these methods are two dimensional gel electrophoresis (2DGE) of proteins and multidimensional liquid chromatographic separation (MuDPIT) of peptides, which rely on isoelectric focusing followed by mass separation in the former, and ion exchange followed by reversed phase separation in the latter. These methods have significant drawbacks in terms of sample bias, sample preparation and reproducibility, and therefore a new methodology that combines the positive aspects of both separation technologies in an automatable, reproducible form is highly desirable. New developments in column technology have allowed rapid improved-resolution separation of intact proteins in complex samples, coupled to improved methodology for peptide and protein identification. The separation of complex protein mixtures using both on- and off-line 2D liquid chromatography using derivitized polystyrene-divinylbenzene (PS-DVB) pellicular ion-exchange resins and PS-DVB monolithic reversed-phase columns is described. Proteolytic digestion of the fractions followed by rapid liquid chromatography-tandem mass spectrometry was used to complete the analysis. An alternative methodology, relying on direct analysis of the second dimension eluents by top-down (mass spectrometric analysis of intact proteins) methodology, using an Apex IV 12 T Fourier-transform ion cyclotron resonance mass spectrometer (Bruker Daltonics) and an HCT ion trap (Bruker Daltonics) equipped with electron transfer dissociation has allowed in-depth analysis of intact proteins. Sample types investigated to establish the utility of the methodology include bacterial lysates (Bordetella parapertusis, and Escherichia coli), a eukaryotic parasite (Leishmania donovani), and transformed human cell lines.
Book Synopsis Lactic Acid Bacteria by : Heping Zhang
Download or read book Lactic Acid Bacteria written by Heping Zhang and published by Springer. This book was released on 2014-04-29 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book summarizes the latest research and developments in dairy biotechnology and engineering. It provides a strategic approach for readers relating to fundamental research and practical work with lactic acid bacteria. The book covers every aspect from identification, ecology, taxonomy and industrial use. All contributors are experts who have substantial experience in the corresponding research field. The book is intended for researchers in the human, animal, and food sciences related to lactic acid bacteria. Dr. Heping Zhang is a Professor at the Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, China. Dr. Yimin Cai works in Livestock and Environment Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Japan.
Book Synopsis On-chip Multi-dimensional Biomolecule Separation Using Multi-layer Microfabricated Valves by : Ying-Chih Wang
Download or read book On-chip Multi-dimensional Biomolecule Separation Using Multi-layer Microfabricated Valves written by Ying-Chih Wang and published by . This book was released on 2004 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent proteomics researches require a sensitive, high-speed, and automatic protein separation tool that far exceeds the capability of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve control in a microchip. Several experiments demonstrating independent 2D separation were performed, and critical parameters for better chip performance were identified; including channel passivation, electroosmosis control, IEF linearity control, and detection enhancement. The result can be used for the filtration of high-abundance proteins, which used to be done by affinity columns. Also, it can be used for much possible integration between different heterogeneous separation/analysis techniques such as IEF, polyacrylamide gel electrophoresis (PAGE), CE, reverse-phase chromatography, and mass spectrometry (MS).
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1876 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Book Synopsis Development of Multi-dimensional Separation Schemes for the Analysis of Membrane Proteins by : Kimberly A. Schneider
Download or read book Development of Multi-dimensional Separation Schemes for the Analysis of Membrane Proteins written by Kimberly A. Schneider and published by . This book was released on 2003 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Characterization by Capillary Isoelectric Focusing Electrophoresis, Reversed Phase Liquid Chromatography and Mass Spectrometry by : Feng Zhou
Download or read book Protein Characterization by Capillary Isoelectric Focusing Electrophoresis, Reversed Phase Liquid Chromatography and Mass Spectrometry written by Feng Zhou and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: With the development of genomics, separation technology and mass spectrometry, proteomics has become an important tool in biological research. Characterization of posttranslational modification (PTM) by mass spectrometry is attracting an increasing attention because of its importance in signal transduction and the difficulty in obtaining this information from genomic methods. Bottom-up methods, which analyze the peptides from enzymatic digestion of proteins, are widely used for proteomic research. However, bottom-up methods may lose some important information when characterizing PTM since not all peptides are detected. In contrast, top-down methods directly characterize intact proteins, which makes it possible to retain PTM information. This dissertation focuses on the characterization of proteins by coupling high throughput methods, capillary isoelectric focusing electrophoresis (CIEF) and electrospray ionization (ESI) mass spectrometry (MS). In this work, a CIEF-RPLC-ESIMS system is designed and constructed for 2-D profiling of protein mixture. The microdialysis membrane based cathodic cell used in this system not only serves as the cathodic cell in CIEF separation, but also transfers the focused proteins separated by CIEF to RPLC. After eliminating the interference of ampholyte, a few fmol of protein can be detected with this system. The detection limit of this system is one order of magnitude lower than 2-D PAGE. By taking the advantage of high resolving power with mass spectrometry and multidimensional separation, the theoretical peak capacity of this system can reach 1x10 6, which is two orders of magnitude higher than theoretical peak capacity of 2-D PAGE. This system is firstly tested with a standard protein mixture. After slight modification, this system is used to profile the intact protein from a yeast enzyme concentrate and a lysate of the green sulfur bacterium Chlorobium tepidum. The pI vs. MW profile obtained from CIEF-RPLC-MS compares favorably with theoretical data derived from C. tepidum genome and experimental data obtained from 2-D-PAGE.
Book Synopsis The Development of Separations and Mass Spectromety [sic] Technologies for the Analysis of Biomolecules by : Maureen Therese Kachman
Download or read book The Development of Separations and Mass Spectromety [sic] Technologies for the Analysis of Biomolecules written by Maureen Therese Kachman and published by . This book was released on 2002 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Two-dimensional Reversed-phase Liquid Chromatography Coupled to MALDI TOF-TOF Mass Spectrometry by : Maria Lasaosa
Download or read book Two-dimensional Reversed-phase Liquid Chromatography Coupled to MALDI TOF-TOF Mass Spectrometry written by Maria Lasaosa and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Recent Advances in Natural Products Analysis by : Seyed Mohammad Nabavi
Download or read book Recent Advances in Natural Products Analysis written by Seyed Mohammad Nabavi and published by Elsevier. This book was released on 2020-03-07 with total page 880 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent Advances in Natural Products Analysis is a thorough guide to the latest analytical methods used for identifying and studying bioactive phytochemicals and other natural products. Chemical compounds, such as flavonoids, alkaloids, carotenoids and saponins are examined, highlighting the many techniques for studying their properties. Each chapter is devoted to a compound category, beginning with the underlying chemical properties of the main components followed by techniques of extraction, purification and fractionation, and then techniques of identification and quantification. Biological activities, possible interactions, levels found in plants, the effects of processing, and current and potential industrial applications are also included. Focuses on the latest analytical techniques used for studying phytochemical and other biological compounds Authored and edited by the top worldwide experts in their field Discusses the current and potential applications and predicts future trends of each compound group
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