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Development Of Methodology For The Study Of Nucleic Acids From Single Cells Using Capillary Electrophoresis
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Book Synopsis Development of Methodology for the Study of Nucleic Acids from Single Cells Using Capillary Electrophoresis by : Jennifer Lee Zabzdyr
Download or read book Development of Methodology for the Study of Nucleic Acids from Single Cells Using Capillary Electrophoresis written by Jennifer Lee Zabzdyr and published by . This book was released on 2004 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson
Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson
Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Humana. This book was released on 2011-12-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single–strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Book Synopsis The Nucleic Acid Protocols Handbook by : Ralph Rapley
Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Book Synopsis Development of New Analytical Method for Specific Nucleic Acid Sequences Using Affinity Capillary Electrophoresis by : 足立賢
Download or read book Development of New Analytical Method for Specific Nucleic Acid Sequences Using Affinity Capillary Electrophoresis written by 足立賢 and published by . This book was released on 2009 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Clinical Applications of Nucleic Acid Amplification by : Meagan B. Myers
Download or read book Clinical Applications of Nucleic Acid Amplification written by Meagan B. Myers and published by Springer Nature. This book was released on 2023-04-11 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Clinical Applications of Nucleic Acid Amplification is a valuable resource for both novice and expert scientists in this developing field.
Book Synopsis Nucleic Acid Electrophoresis by : Dietmar Tietz
Download or read book Nucleic Acid Electrophoresis written by Dietmar Tietz and published by Springer. This book was released on 2012-11-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described here in detail. Leading experts present the required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers' addresses, helpful Internet resources, as well as specific applications, e.g. in legal medicine, microbiology and agriculture.
Book Synopsis Gel Electrophoresis: Nucleic Acids by : Dr Robin Martin
Download or read book Gel Electrophoresis: Nucleic Acids written by Dr Robin Martin and published by Garland Science. This book was released on 2020-08-26 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes.
Book Synopsis Footprinting of Nucleic Acid-Protein Complexes by :
Download or read book Footprinting of Nucleic Acid-Protein Complexes written by and published by Academic Press. This book was released on 2014-05-19 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Footprinting of Nucleic Acid-Protein Complexes provides protocols for studying the stoichiometry, binding site size and location, and structural changes in nucleic acids caused by their interaction with proteins. The methods are central to studying key biological processes, such as transcription and translation. The techniques are important to experiments in vivoand in vitro, in eukaryotes and in prokaryotes, at qualitative and quantitative levels,and across many disciplines. This book is a laboratory manual of footprinting techniques for studying nucleic acid-protein interactions. It contains clear and concise descriptions of the most important methodologies, and includes in vivo as well as in vitro applications. It is aimed at bench scientists from graduate students on, and should be of value in industrial labs as well as in academic settings. Use of different footprinting approaches can provide unique insights into DNA-protein systems. The protocols containedin this handbook are written to be"user-friendly,"and thus should be conducive to extending the use of footprinting to new systems. The section on quantitative analysis of DNAse I footprints should prove especially useful for in depth evaluation of cooperative interactions. (For the End User) Provides clear exposition of footprinting techniques for characterizing DNA-protein interactions Covers both protection methods for identifying sites of protein binding and interference methods for determining points of contact between DNA and protein Includes approaches for both in vitro and in vivo measurements High quality, timely, and of lasting practical value in the laboratory
Book Synopsis Capillary Electrophoresis of Nucleic Acids by :
Download or read book Capillary Electrophoresis of Nucleic Acids written by and published by Springer. This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Book Synopsis Nucleic Acid Electrophoresis by : Dietmar Tietz
Download or read book Nucleic Acid Electrophoresis written by Dietmar Tietz and published by . This book was released on 1998-10-22 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA).
Book Synopsis Capillary Electrophoresis-based Methodology Development for Biomolecule Analysis by : NI. Li
Download or read book Capillary Electrophoresis-based Methodology Development for Biomolecule Analysis written by NI. Li and published by . This book was released on 2011 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) is a separation tool with wide applications in biomolecule analysis. Fast and high-resolution separation requiring minute sample volumes is advantageous to study multiple components in biological samples. Flexible modes and methods can be developed. In this thesis, I focus on developing and applying novel CE methods to study multi-target nucleic acid sensing with high sensitivity (Part I) and interactions between multiple components, i.e. proteins, nanoparticles and drugs (Part II).
Book Synopsis Analysis of Nucleic Acids by Capillary Electrophoresis by : Christoph Heller
Download or read book Analysis of Nucleic Acids by Capillary Electrophoresis written by Christoph Heller and published by Springer Science & Business Media. This book was released on 1997-06-26 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last decade, capillary electrophoresis has been developed into a very powerful analytical technique, which has many advantages over conventional slab gel techniques. The improvement is comparable to the one occuring earlier in the field of chromatography, with the introduction of the high performance technology. How important this technique has be come, is reflected by the shear volume of papers published each month; as well as a dozen of books already published on this subject. One of the most important conferences in the field, the "International Symposium on High Performance Capillary Electrophoresis" attracts now nearly thousand people every year. As capillary electrophoresis can be applied to many different analytical problems, a spe cialization is unavoidable. This evolution is also reflected in the development of instrumen tation: whereas the first devices were designed for all possible applications, new instruments are now built, that are specialized for one particular task, e.g. DNA analysis. I very much welcome the decision of the series editor and the publisher, to edit a series ofspecialized books, covering all aspects of capillary electrophoresis. Having worked on the electrophoretic separation of DNA for many years, I am convinced that there are so many different aspects on this issue that they deserve a whole book on their own. Therefore, I was happy to agree when being asked to edit this book.
Book Synopsis Gel Electrophoresis: Nucleic Acids by : P. Jones
Download or read book Gel Electrophoresis: Nucleic Acids written by P. Jones and published by Wiley-Blackwell. This book was released on 1995 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gel electrophoresis of nucleic acids is the one technique that spans the whole range of molecular biology techniques. The combination of its high resolution and versatility of its applications makes it the one method used by all molecular biologists. This book gives clear, step-by-step protocols for all the important techniques from simple analytical separations of nucleic acids to the latest PCR techniques. Hence it will be essential reading for all those working in the area of molecular biology. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
Book Synopsis Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis by : Woei G. Tan
Download or read book Rapid Sizing of DNA and Analysis of Single Cells Using Capillary Electrophoresis written by Woei G. Tan and published by . This book was released on 2000 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Molecular Biology: Capillary electrophoresis of nucleic acids: Introduction to the capillary electrophoresis of nucleic acids by : John M. Walker
Download or read book Methods in Molecular Biology: Capillary electrophoresis of nucleic acids: Introduction to the capillary electrophoresis of nucleic acids written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
