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Author : Mandi L. Conrad
Publisher :
ISBN 13 :
Total Pages : 318 pages
Book Rating : 4.:/5 (259 download)
Download or read book Development of Analytical Uses of High Performance Affinity Chromatography written by Mandi L. Conrad and published by . This book was released on 2008 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Irwin M. Chaiken
Publisher : CRC Press
ISBN 13 : 1351078127
Total Pages : 204 pages
Book Rating : 4.3/5 (51 download)
Download or read book Analytical Affinity Chromatography written by Irwin M. Chaiken and published by CRC Press. This book was released on 2018-01-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).
Author : Robert L. Wixom
Publisher : John Wiley & Sons
ISBN 13 : 1118060296
Total Pages : 357 pages
Book Rating : 4.1/5 (18 download)
Download or read book Chromatography written by Robert L. Wixom and published by John Wiley & Sons. This book was released on 2011-01-31 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading researchers discuss the past and present of chromatography More than one hundred years after Mikhail Tswett pioneered adsorption chromatography, his separation technique has developed into an important branch of scientific study. Providing a full portrait of the discipline, Chromatography: A Science of Discovery bridges the gap between early, twentieth-century chromatography and the cutting edge of today’s research. Featuring contributions from more than fifty award-winning chromatographers, Chromatography offers a multifaceted look at the development and maturation of this field into its current state, as well as its importance across various scientific endeavors. The coverage includes: Consideration of chromatography as a unified science rather than just a separation method Key breakthroughs, revolutions, and paradigm shifts in chromatography Profiles of Nobel laureates who used chromatography in their research, and the role it played Recent advances in column technology Chromatography’s contributions to the agricultural, space, biological/medical sciences; pharmaceutical science; and environmental, natural products, and chemical analysis Future trends in chromatography With numerous references and an engaging series of voices, Chromatography: A Science of Discovery offers a diverse look at an essential area of science. It is a unique and invaluable resource for researchers, students, and other interested readers who seek a broader understanding of this field.
Author : Allan Matte
Publisher : Elsevier
ISBN 13 : 0081030193
Total Pages : 222 pages
Book Rating : 4.0/5 (81 download)
Download or read book Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics written by Allan Matte and published by Elsevier. This book was released on 2020-09-07 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Author : Irwin Chaiken
Publisher : Elsevier
ISBN 13 : 0323146627
Total Pages : 542 pages
Book Rating : 4.3/5 (231 download)
Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Author : David S. Hage
Publisher : CRC Press
ISBN 13 : 0824751981
Total Pages : 878 pages
Book Rating : 4.8/5 (247 download)
Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Author : Erika L. Pfaunmiller
Publisher :
ISBN 13 : 9781321299625
Total Pages : 252 pages
Book Rating : 4.2/5 (996 download)
Download or read book Development of Novel Monoliths and Affinity Microcolumns for Use in High Performance Affinity Chromatography and Pharmaceutical Applications written by Erika L. Pfaunmiller and published by . This book was released on 2014 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance affinity chromatography (HPAC) is a useful tool for probing biological interactions such as the binding of an antibody with an antigen or the binding of a drug with a serum protein. This dissertation describes the development of affinity microcolumns for the rapid analysis of various analytes and the extension of some of these microcolumns for use with immunoaffinity chromatography (IAC).
Author : Csaba Horváth
Publisher : Elsevier
ISBN 13 : 1483261840
Total Pages : 342 pages
Book Rating : 4.4/5 (832 download)
Download or read book High-Performance Liquid Chromatography written by Csaba Horváth and published by Elsevier. This book was released on 2013-10-22 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-Performance Liquid Chromatography: Advances and Perspectives, Volume 5 presents the applications of high-performance liquid chromatography to the analysis and purification of biopolymers. The book, composed of three chapters, provides a detailed description of silica gel-supported stationary phases; tackles biospecific interaction chromatography, a tool in the study of complex carbohydrates and as an industrial separation process in biotechnology; and discusses the potential advantages of displacement chromatography for multicomponent separations. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good source of information.
Author : Phyllis R. Brown
Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 710 pages
Book Rating : 4.3/5 (91 download)
Download or read book High Performance Liquid Chromatography written by Phyllis R. Brown and published by Wiley-Interscience. This book was released on 1989-01-19 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detectors for Liquid Chromatography Edited by Edward S. Yeung Written by an expert in the field, this comprehensive guide explains the basic principles behind detector response instrumentation and selected applications. Early chapters cover absorption detectors for high performance liquid chromatography, FTIR detection, indirect absorbance detectors, fluorometric detection, and polarimetric detectors. Coverage continues with detection based on electrical and electrochemical measurements, mass spectrometry as an online detector for HPLC, and miscellaneous methods. 1986 0 471-82169-1 366 pp. Small Bore High Performance Liquid Chromatography Edited by Raymond R. W. Scott A state-of-the-art guide that demonstrates how to design, construct, and pack optimized small bore columns, the center of any chromatography system. Case examples show the use of these columns for high resolution, very fast analysis, and special methods for molecular weight determinations. Applications from a wide range of industrial and forensic analyses aid in developing sophistication in a number of useful techniques. The book provides essential information on topics such as calculating the minimum column radius, detectors, and molecular diffusion. Includes 92 illustrations and 14 tables to enhance explanations of microbore HPLC methods. 1984 0 471-80052-X 271 pp. Reversed-Phase High Performance Liquid Chromatography: Theory, Practice, and Biomedical Applications Ante M. Krstulovic and Phyllis R. Brown Reversed-phase liquid chromatography has increased tremendously in popularity over the past ten years. Estimates show that more than 80% of all HPLC separations are performed using this technique. This book covers both theoretical aspects of RPLC and practical information needed in diverse areas of research; it also contains a review of the RPLC applications in the biomedical/biochemical field, with references and collateral readings. Material is presented in a practical, problem-solving manner and should be immensely useful in theoretical aspects of RPLC and all areas of scientific research, particularly the biomedical/biochemical field, where RPLC has made its largest impact. 1982 0 471-05369-4 296 pp.
Author : Michael Zachariou
Publisher : Springer Science & Business Media
ISBN 13 : 1588296598
Total Pages : 341 pages
Book Rating : 4.5/5 (882 download)
Download or read book Affinity Chromatography written by Michael Zachariou and published by Springer Science & Business Media. This book was released on 2008 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.
Author : Danilo Corradini
Publisher : CRC Press
ISBN 13 : 1420016946
Total Pages : 715 pages
Book Rating : 4.4/5 (2 download)
Download or read book Handbook of HPLC written by Danilo Corradini and published by CRC Press. This book was released on 2016-04-19 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the
Author : P. Mohr
Publisher : CRC Press
ISBN 13 : 9780824774684
Total Pages : 320 pages
Book Rating : 4.7/5 (746 download)
Download or read book Affinity Chromatography written by P. Mohr and published by CRC Press. This book was released on 1985-11-22 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.
Author : Shiden T. Azaria
Publisher :
ISBN 13 :
Total Pages : 97 pages
Book Rating : 4.:/5 (12 download)
Download or read book Development of Entrapment Columns for the Study of Affinity Based Analysis of Drug-protein Interactions written by Shiden T. Azaria and published by . This book was released on 2017 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-performance affinity chromatography (HPAC) is a type of liquid chromatography in which solutes are separated based on their binding to a stationary phase that is a biologically-related agent. Because of the strong and selective nature of many biological interactions, this method has already become a powerful technique for the purification and analysis of solutes that are complementary to the immobilized binding agent. Human serum albumin (HSA), the most abundant protein in the blood with concentrations of 35-50 mg/mL in serum, has interactions with many drugs, which can affect the absorption, distribution, metabolism and excretion of such agents. The overall goal of this thesis is to examine the use of on-column entrapment methods based on hydrazide-activated silica and oxidized glycogen as a capping agent for the immobilization of proteins as affinity ligands in HPAC. Although this general type of entrapment method has been previously examined reported by our group, this method still needs further optimization for its use in an on-column format and in new applications based on HPAC. For example, it is necessary to conduct studies to further increase the amount of the entrapped affinity ligand that can be obtained by using alternative types of supports.
Author : David S. Hage
Publisher : CRC Press
ISBN 13 : 9780824740573
Total Pages : 968 pages
Book Rating : 4.7/5 (45 download)
Download or read book Handbook of Affinity Chromatography, Second Edition written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatography reflects upon important factors to consider in the development of affinity methods, such as the choice of support material, immobilization methods, and application or elution conditions. It reviews common affinity methods and explores the latest preparative, analytical, and biophysical applications, including the use of affinity chromatography with other separation techniques and analytical systems. This basis seamlessly supports the discussion of recent developments in techniques including the use of affinity ligands in capillary electrophoresis, mass spectrometry, microanalytical systems, and optical biosensors. New chapters feature expanded discussions on molecularly imprinted polymers and biomimetic ligands, chromatographic immunoassays, affinity-based immunoassays, affinity-based chiral stationary phases, and affinity ligands in multidimensional systems. Engaging the collaboration of 48 scientists and students from 23 laboratories and organizations to present the latest information on affinity methods, the Handbook of Affinity Chromatography illustrates a wide range of applications and theory for scientists, students, and laboratory workers throughout the fields of chemistry and biology.
Author : Salvatore Fanali
Publisher : Elsevier
ISBN 13 : 0323999999
Total Pages : 886 pages
Book Rating : 4.3/5 (239 download)
Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2023-04-20 with total page 886 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Fundamentals and Instrumentation, Third Edition offers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The book gives those working in academia and industry the opportunity to learn, refresh, and deepen their understanding of the field by covering basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. This third edition addresses new developments in the field with updated chapters from expert researchers. The book is a valuable reference for research scientists, teachers, university students, industry professionals in research and development, and quality control managers. Emphasizes the integration of chromatographic methods and sample preparation Provides important data related to complex matrices, sample preparation, and data handling Gives background information to facilitate the choice of LC sub-technique and experimental conditions, mobile and stationary phases, detectors, data processing, and more Offers comprehensive updates to all chapters Includes new chapters on chiral recognition, co-solvents and mobile phase additives, physicochemical measurements, and identification and quantitation in mass spectrometry
Author : Salvatore Fanali
Publisher : Elsevier
ISBN 13 : 0323983006
Total Pages : 1086 pages
Book Rating : 4.3/5 (239 download)
Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2023-04-20 with total page 1086 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Applications, Third Edition delivers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The text gives those working in academia and industry the opportunity to learn, refresh, and deepen their understanding of the field by covering basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. This third edition addresses new developments in the field with updated chapters from expert researchers. The book is a valuable reference for research scientists, teachers, university students, industry professionals in research and development, and quality control managers. Emphasizes the integration of chromatographic methods and sample preparation Provides important data related to complex matrices, sample preparation, and data handling Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Offers comprehensive updates to all chapters Adds new chapters on selection of liquid chromatographic mode, proteomics, doping analysis, analysis of microplastics, and analysis of pharmaceutically and biologically relevant isoforms
Author : Hassan Y. Aboul-Enein
Publisher : CRC Press
ISBN 13 : 1000146251
Total Pages : 482 pages
Book Rating : 4.0/5 (1 download)
Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-15 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.
