Development And Validation Of A Hydrostatic Pressure Bioreactor For Cell Culture Applications In Intervertebral Disc And Mesenchymal Stem Cells

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Development and Validation of a Hydrostatic Pressure Bioreactor for Cell Culture: Applications in Intervertebral Disc and Mesenchymal Stem Cells

Author : Troy Justin Eggum
Publisher :
ISBN 13 : 9780494263402
Total Pages : 120 pages
Book Rating : 4.2/5 (634 download)

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Book Synopsis Development and Validation of a Hydrostatic Pressure Bioreactor for Cell Culture: Applications in Intervertebral Disc and Mesenchymal Stem Cells by : Troy Justin Eggum

Download or read book Development and Validation of a Hydrostatic Pressure Bioreactor for Cell Culture: Applications in Intervertebral Disc and Mesenchymal Stem Cells written by Troy Justin Eggum and published by . This book was released on 2007 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Degeneration of the intervertebral disc (IVD) is a significant cause of pain and immobility for many people. Tissue engineering with mesenchymal stem cells (MSC) offers promise for repair or regeneration of disc tissue if they can be encouraged to differentiate to a nucleus pulposus phenotype. The cells were exposed to conditions approximating the in vivo extracellular environment of the IVD; low glucose (2.5 mg/mL), 5% hypoxia, and medium conditioned by the cells of the nucleus pulposus (notochordal and chondrocyte-like). A novel hydrostatic pressure bioreactor was designed and validated to simulate physiologic-range pressures (1MPa at 0.5 Hz and static) to in vitro cultures of MSC. These conditions were compared to the "standard" cell culture conditions of 4.5 mg/mL glucose, 20% oxygen, no hydrostatic pressure, and a "neutral" conditioned medium. The bioreactor was shown to not adversely affect cell culture and MSC were sensitive to all factors tested.

The Development and Validation of a Hydrostatic Pressure Bioreactor for Applications in Bone Tissue Engineering

Author : Joshua Colm Felician Aeddan Price
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (16 download)

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Book Synopsis The Development and Validation of a Hydrostatic Pressure Bioreactor for Applications in Bone Tissue Engineering by : Joshua Colm Felician Aeddan Price

Download or read book The Development and Validation of a Hydrostatic Pressure Bioreactor for Applications in Bone Tissue Engineering written by Joshua Colm Felician Aeddan Price and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Bioreactors for Stem Cell Expansion and Differentiation

Author : Joaquim M.S. Cabral
Publisher : CRC Press
ISBN 13 : 0429841817
Total Pages : 260 pages
Book Rating : 4.4/5 (298 download)

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Book Synopsis Bioreactors for Stem Cell Expansion and Differentiation by : Joaquim M.S. Cabral

Download or read book Bioreactors for Stem Cell Expansion and Differentiation written by Joaquim M.S. Cabral and published by CRC Press. This book was released on 2018-09-03 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Bioreactor Systems for Tissue Engineering

Author : Cornelia Kasper
Publisher : Springer Science & Business Media
ISBN 13 : 3540693564
Total Pages : 274 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis Bioreactor Systems for Tissue Engineering by : Cornelia Kasper

Download or read book Bioreactor Systems for Tissue Engineering written by Cornelia Kasper and published by Springer Science & Business Media. This book was released on 2009-02-03 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: The editors of this special volume would first like to thank all authors for their excellent contributions. We would also like to thank Prof. Dr. Thomas Scheper, Dr. Marion Hertel and Ulrike Kreusel for providing the opportunity to compose this volume and Springer for organizational and technical support. Tissue engineering represents one of the major emerging fields in modern b- technology; it combines different subjects ranging from biological and material sciences to engineering and clinical disciplines. The aim of tissue engineering is the development of therapeutic approaches to substitute diseased organs or tissues or improve their function. Therefore, three dimensional biocompatible materials are seeded with cells and cultivated in suitable systems to generate functional tissues. Many different aspects play a role in the formation of 3D tissue structures. In the first place the source of the used cells is of the utmost importance. To prevent tissue rejection or immune response, preferentially autologous cells are now used. In particular, stem cells from different sources are gaining exceptional importance as they can be differentiated into different tissues by using special media and supplements. In the field of biomaterials, numerous scaffold materials already exist but new composites are also being developed based on polymeric, natural or xenogenic sources. Moreover, a very important issue in tissue en- neering is the formation of tissues under well defined, controlled and reprod- ible conditions. Therefore, a substantial number of new bioreactors have been developed.

Stem Cell Bioprocessing and Manufacturing

Author : Joaquim M. S. Cabral
Publisher : MDPI
ISBN 13 : 3039430386
Total Pages : 170 pages
Book Rating : 4.0/5 (394 download)

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Book Synopsis Stem Cell Bioprocessing and Manufacturing by : Joaquim M. S. Cabral

Download or read book Stem Cell Bioprocessing and Manufacturing written by Joaquim M. S. Cabral and published by MDPI. This book was released on 2021-01-20 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: The next healthcare revolution will apply regenerative medicines using human cells and tissues. The aim of the regenerative medicine approach is to create biological therapies or substitutes in vitro for the replacement or restoration of tissue function in vivo lost through failure or disease. However, whilst science has revealed the potential, and early products have shown the power of such therapies, there is an immediate and long-term need for expertise with the necessary skills to face the engineering and life science challenges before the predicted benefits in human healthcare can be realized. Specifically, there is a need for the development of bioprocess technology for the successful transfer of laboratory-based practice of stem cell and tissue culture to the clinic as therapeutics through the application of engineering principles and practices. This Special Issue of Bioengineering on Stem Cell Bioprocessing and Manufacturing addresses the central role in defining the engineering sciences of cell-based therapies, by bringing together contributions from worldwide experts on stem cell biology and engineering, bioreactor design and bioprocess development, scale-up, and manufacturing of stem cell-based therapies.

Bioreactors for Tissue Engineering

Author : Julian Chaudhuri
Publisher : Springer Science & Business Media
ISBN 13 : 1402037414
Total Pages : 373 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Bioreactors for Tissue Engineering by : Julian Chaudhuri

Download or read book Bioreactors for Tissue Engineering written by Julian Chaudhuri and published by Springer Science & Business Media. This book was released on 2006-01-16 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the first time in a single volume, the design, characterisation and operation of the bioreactor system in which the tissue is grown is detailed. Bioreactors for Tissue Engineering presents an overall picture of the current state of knowledge in the engineering of bioreactors for several tissue types (bone, cartilage, vascular), addresses the issue of mechanical conditioning of the tissue, and describes the use of techniques such as MRI for monitoring tissue growth. This unique volume is dedicated to the fundamentals and application of bioreactor technology to tissue engineering products. Not only will it appeal to graduate students and experienced researchers in tissue engineering and regenerative medicine, but also to tissue engineers and culture technologists, academic and industrial chemical engineers, biochemical engineers and cell biologists who wish to understand the criteria used to design and develop novel systems for tissue growth in vitro.

Bioreactor Systems for Tissue Engineering II

Author : Cornelia Kasper
Publisher : Springer Science & Business Media
ISBN 13 : 3642160506
Total Pages : 331 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Bioreactor Systems for Tissue Engineering II by : Cornelia Kasper

Download or read book Bioreactor Systems for Tissue Engineering II written by Cornelia Kasper and published by Springer Science & Business Media. This book was released on 2010-10-03 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alternative Sources of Adult Stem Cells: Human Amniotic Membrane, by S. Wolbank, M. van Griensven, R. Grillari-Voglauer, and A. Peterbauer-Scherb; * Mesenchymal Stromal Cells Derived from Human Umbilical Cord Tissues: Primitive Cells with Potential for Clinical and Tissue Engineering Applications, by P. Moretti, T. Hatlapatka, D. Marten, A. Lavrentieva, I. Majore, R. Hass and C. Kasper; * Isolation, Characterization, Differentiation, and Application of Adipose-Derived Stem Cells, by J. W. Kuhbier, B. Weyand, C. Radtke, P. M. Vogt, C. Kasper and K. Reimers; * Induced Pluripotent Stem Cells: Characteristics and Perspectives, by T. Cantz and U. Martin; * Induced Pluripotent Stem Cell Technology in Regenerative Medicine and Biology, by D. Pei, J. Xu, Q. Zhuang, H.-F. Tse and M. A. Esteban; * Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells, by C. Weber, S. Pohl, R. Poertner, P. Pino-Grace, D. Freimark, C. Wallrapp, P. Geigle and P. Czermak; * Cartilage Engineering from Mesenchymal Stem Cells, by C. Goepfert, A. Slobodianski, A.F. Schilling, P. Adamietz and R. Poertner; * Outgrowth Endothelial Cells: Sources, Characteristics and Potential Applications in Tissue Engineering and Regenerative Medicine, by S. Fuchs, E. Dohle, M. Kolbe, C. J. Kirkpatrick; * Basic Science and Clinical Application of Stem Cells in Veterinary Medicine, by I. Ribitsch, J. Burk, U. Delling, C. Geißler, C. Gittel, H. Jülke, W. Brehm; * Bone Marrow Stem Cells in Clinical Application: Harnessing Paracrine Roles and Niche Mechanisms, by R. M. El Backly, R. Cancedda; * Clinical Application of Stem Cells in the Cardiovascular System, C. Stamm, K. Klose, Y.-H. Choi

Cell and Tissue Reaction Engineering

Author : Regine Eibl
Publisher : Springer Science & Business Media
ISBN 13 : 3540681825
Total Pages : 367 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis Cell and Tissue Reaction Engineering by : Regine Eibl

Download or read book Cell and Tissue Reaction Engineering written by Regine Eibl and published by Springer Science & Business Media. This book was released on 2008-09-30 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.

Bioprocessing for Cell-Based Therapies

Author : Che J. Connon
Publisher : John Wiley & Sons
ISBN 13 : 1118743407
Total Pages : 216 pages
Book Rating : 4.1/5 (187 download)

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Book Synopsis Bioprocessing for Cell-Based Therapies by : Che J. Connon

Download or read book Bioprocessing for Cell-Based Therapies written by Che J. Connon and published by John Wiley & Sons. This book was released on 2016-12-15 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions from leading, international academics and industrial practitioners, Bioprocessing for Cell-Based Therapies explores the very latest techniques and guidelines in bioprocess production to meet safety, regulatory and ethical requirements, for the production of therapeutic cells, including stem cells. An authoritative, cutting-edge handbook on bioprocessing for the production of therapeutic cells with extensive illustrations in full colour throughout An authoritative, cutting-edge handbook on bioprocessing for the production of therapeutic cells with extensive illustrations in full colour throughout In depth discussion of the application of cell therapy including methods used in the delivery of cells to the patient Includes contributions from experts in both academia and industry, combining a practical approach with cutting edge research The only handbook currently available to provide a state of the art guide to Bioprocessing covering the complete range of cell-based therapies, from experts in academia and industry

Design and Validation of a Low-cost Bioreactor System for Therapeutic Mesenchymal Stromal Cell Expansion

Author : Morgan Elizabeth Mantay
Publisher :
ISBN 13 :
Total Pages : 228 pages
Book Rating : 4.:/5 (112 download)

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Book Synopsis Design and Validation of a Low-cost Bioreactor System for Therapeutic Mesenchymal Stromal Cell Expansion by : Morgan Elizabeth Mantay

Download or read book Design and Validation of a Low-cost Bioreactor System for Therapeutic Mesenchymal Stromal Cell Expansion written by Morgan Elizabeth Mantay and published by . This book was released on 2019 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of stem cells for clinical applications has increased as biomedical researchers develop cell-based therapeutic approaches for use in tissue regeneration and/or the treatment of medical conditions such as diabetes or other diseases. Large numbers of stem cells are required as research progresses from the laboratory to clinical trials and on to subsequent industrial scale-up for large-scale manufacturing for distribution. There are a wide range of bioreactors available on the market for anchorage-dependent stem cell expansion, however, to increase accessibility and affordability of cell therapies, it is critical to consider the bioprocesses associated with culturing to support cell expansion and quality. In this work, we aimed to design a low-cost bioreactor system for as mesenchymal stromal cell (MSC) expansion that yields clinically relevant numbers of cells with reduced labor, while maintaining cell potency and immunomodulatory capabilities essential for successful clinical application.

Design of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply

Author : Manuel Kohlmann
Publisher : Diplomica Verlag
ISBN 13 : 3842858949
Total Pages : 107 pages
Book Rating : 4.8/5 (428 download)

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Book Synopsis Design of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply by : Manuel Kohlmann

Download or read book Design of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply written by Manuel Kohlmann and published by Diplomica Verlag. This book was released on 2011-03 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Perfused bioreactors have been developed with different designs and with various cell lines such as fibroblasts, osteoblasts and C2C12 myoblasts. All these current systems are custom made and mostly capable for only one cell type. Small seeding spaces and chambers inhibit longer cell studies and the observation of cell interaction. This study presents an advanced perfusion bioreactor which allows cell observation for longer than 5 days on an area of 2.8cm2 with a volume of 17ml. Initial experiments investigated homogeneity of the heat distribution, which is precisely controlled and stable over the length of the experiment. The addition of perfusion to the system results in the manual feeding process with modified F12 nutrient media being unnecessary. The perfusion and perfusion rate are user controllable up to 8ml/h. Different inlets allow cell seeding, cell feeding and chemical stimulation. Since the metabolism by-products are diluted and removed by the flow of the perfused system, no inhibited growth occurs and the pH-value will maintain constant at 7.4 which removes the need for balancing the CO2 environment. Fully enclosed and sealed assemblies with a controlled hot plate can be used outside of the incubator and incorporated into the stage of a microscope to track and monitor cell growth. The bioreactor chamber consists of three parts of transparent annealed cast Acrylic plus sealing material. Acrylic is chosen since it is machinable by laser cutting, which is a fast and easy method of manufacture. Due to the annealing process sterilizing by ethanol is possible. Heat distribution analysis was made with an IR-camera. And the pH was tested by indicator paper. The flow rate was set at 3.3ml/h. Computer simulations for flow and heat distribution and standard tests with cell cultures showed that a round bioreactor chamber design has advantages due to more uniform conditions. To track the cells during their distribution and over their whole life cycle, a completely transparent system is being developed. It includes an Indium Tin Oxide (ITO) coated glass plate, which can be used as a hot plate with precise controlled heating properties to heat the whole chamber.

Bioreactors

Author : Luigi Salvay
Publisher :
ISBN 13 : 9781681176956
Total Pages : 304 pages
Book Rating : 4.1/5 (769 download)

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Book Synopsis Bioreactors by : Luigi Salvay

Download or read book Bioreactors written by Luigi Salvay and published by . This book was released on 2017-02 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Bioreactors in Stem Cell Biology

Author : Kursad Turksen
Publisher :
ISBN 13 : 9781071620182
Total Pages : 273 pages
Book Rating : 4.6/5 (21 download)

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Book Synopsis Bioreactors in Stem Cell Biology by : Kursad Turksen

Download or read book Bioreactors in Stem Cell Biology written by Kursad Turksen and published by . This book was released on 2022 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioreactors in Stem Cell Biology: Methods and Protocols, Second Edition will benefit both established investigators and newcomers to this dynamic area of study.

Characterization and Impact of the Hydrodynamics on the Performance of Umbilical-cord Derived Stem Cells Culture in Stirred Tank Bioreactors

Download Characterization and Impact of the Hydrodynamics on the Performance of Umbilical-cord Derived Stem Cells Culture in Stirred Tank Bioreactors PDF Online Free

Author : Céline Loubière
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (18 download)

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Book Synopsis Characterization and Impact of the Hydrodynamics on the Performance of Umbilical-cord Derived Stem Cells Culture in Stirred Tank Bioreactors by : Céline Loubière

Download or read book Characterization and Impact of the Hydrodynamics on the Performance of Umbilical-cord Derived Stem Cells Culture in Stirred Tank Bioreactors written by Céline Loubière and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal stem cells (MSC) are becoming increasingly involved in the regenerative medicine field, particularly to treat diseases that are not effectively curable with the current therapies. Two scientific barriers are nevertheless responsible for MSC use and commercialization limitations. On one side, large amounts of cells are needed to reach the high cell dose requirements. On the other side, cells being the final product themselves, directly injected into the patient, their quality have to be controlled (stem cell phenotype, differentiation capability). MSC cultivation on microcarriers in a stirred bioreactor seems to meet these challenges. However, a precise knowledge about the impact of the technologies and the hydrodynamics generated, on the physiological cell response, is necessary to improve the scale-up of MSC cultures in bioreactors. In this context, present work is dedicated to the study of the impact of the agitation mode (orbital or mechanical) on the cell attachment, expansion and detachment on various microcarrier types, in the case of MSC derived from the Wharton's jelly (WJ-MSC) of umbilical cords. To quantify more precisely cell distribution and expansion on microcarriers, an automatic and in situ counting method was developed, which need no detachment step. This allowed the identification of commercial microcarriers suitable for WJ-MSC cultures, which were then compared to home-made microcarriers, synthesized by a partner laboratory, in terms of cell attachment and expansion, and detachment efficiency. In parallel to these works, the impact of the impeller design on the microcarrier suspension in stirred tank bioreactors was investigated. Based on a dimensional analysis and CFD simulations, it resulted in the establishment of two models relating the minimal agitation rate to ensure all particle suspension (Njs) with the impeller geometrical characteristics (design, size, off-bottom clearance) and the material properties of both the solid and the liquid phases. CFD models validation allowed then to develop a strategy to optimize the geometrical configuration of an impeller, dedicated to MSC cultures on microcarriers in a minibioreactor. Parameters characterizing the hydromechanical stress encountered by the solid phase were wisely chosen and integrated into CFD simulations. Based on a design of experiments, and the hydrodynamics data recovered from simulations, response surfaces were built and a multiobjective optimization was achieved in order to determine the geometry minimizing the particle stress, and also by adhered cells. WJ-MSC cultures in minibioreactors equipped with impellers displaying various geometries were finally validated, with a preliminary comparison of the impact of these geometries on the cell expansion.

Intervertebral Disc Regeneration

Author : Benjamin Gantenbein
Publisher : Mdpi AG
ISBN 13 : 9783036527543
Total Pages : 138 pages
Book Rating : 4.5/5 (275 download)

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Book Synopsis Intervertebral Disc Regeneration by : Benjamin Gantenbein

Download or read book Intervertebral Disc Regeneration written by Benjamin Gantenbein and published by Mdpi AG. This book was released on 2022-01-11 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a brief snapshot of recent research on the subject of intervertebral disc degeneration and how this specific organ could be regenerated. It provides stimuli to the reader in representing research from different angles in this cross-disciplinary field of spine surgeons, mechanical engineers and biologists. In particular, it is instructive as to how disc herniations could be successfully induced in vitro and, also, to how novel cell-based therapies using rare autochthonous stem cells could potentially be used in the future.

Functional Tissue Engineering

Author : Farshid Guilak
Publisher : Springer Science & Business Media
ISBN 13 : 0387955534
Total Pages : 434 pages
Book Rating : 4.3/5 (879 download)

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Book Synopsis Functional Tissue Engineering by : Farshid Guilak

Download or read book Functional Tissue Engineering written by Farshid Guilak and published by Springer Science & Business Media. This book was released on 2003-07-09 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: -Softcover reprint of a successful hardcover reference (370 copies sold) -Price to be accessible to the rapidly increasing population of students and investigators in the field of tissue engineering -Chapters written by well-known researchers discuss issues in functional tissue engineering as well as provide guidelines and a summary of the current state of technology

Cell Engineering and Regeneration

Author : Heinz Redl
Publisher : Springer
ISBN 13 : 9783319088303
Total Pages : 0 pages
Book Rating : 4.0/5 (883 download)

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Book Synopsis Cell Engineering and Regeneration by : Heinz Redl

Download or read book Cell Engineering and Regeneration written by Heinz Redl and published by Springer. This book was released on 2017-02-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference work presents the origins of cells for tissue engineering and regeneration, including primary cells, tissue-specific stem cells, pluripotent stem cells and trans-differentiated or reprogrammed cells. There is particular emphasis on current understanding of tissue regeneration based on embryology and evolution studies, including mechanisms of amphibian regeneration. The book covers the use of autologous versus allogeneic cell sources, as well as various procedures used for cell isolation and cell pre-conditioning , such as cell sorting, biochemical and biophysical pre-conditioning, transfection and aggregation. It also presents cell modulation using growth factors, molecular factors, epigenetic approaches, changes in biophysical environment, cellular co-culture and other elements of the cellular microenvironment. The pathways of cell delivery are discussed with respect to specific clinical situations, including delivery of ex vivo manipulated cells via local and systemic routes, as well as activation and migration of endogenous reservoirs of reparative cells. The volume concludes with an in-depth discussion of the tracking of cells in vivo and their various regenerative activities inside the body, including differentiation, new tissue formation and actions on other cells by direct cell-to-cell communication and by secretion of biomolecules.