Development And Application Of Mass Spectrometry In Monitoring Of Chemical Reactions And Rapid Characterization Of Pathogenic Bacteria

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MALDI-TOF and Tandem MS for Clinical Microbiology

Author : Haroun N. Shah
Publisher : John Wiley & Sons
ISBN 13 : 1118960238
Total Pages : 850 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis MALDI-TOF and Tandem MS for Clinical Microbiology by : Haroun N. Shah

Download or read book MALDI-TOF and Tandem MS for Clinical Microbiology written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2017-03-31 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

Indicators for Waterborne Pathogens

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309091225
Total Pages : 329 pages
Book Rating : 4.3/5 (9 download)

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Book Synopsis Indicators for Waterborne Pathogens by : National Research Council

Download or read book Indicators for Waterborne Pathogens written by National Research Council and published by National Academies Press. This book was released on 2004-06-19 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens.Â Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples.Â This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application.Â The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â

Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry

Author : Rainer Cramer
Publisher : Springer
ISBN 13 : 3319048198
Total Pages : 287 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry by : Rainer Cramer

Download or read book Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry written by Rainer Cramer and published by Springer. This book was released on 2015-11-09 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics

Time-Resolved Mass Spectrometry

Author : Pawel L. Urban
Publisher : John Wiley & Sons
ISBN 13 : 1118887352
Total Pages : 376 pages
Book Rating : 4.1/5 (188 download)

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Book Synopsis Time-Resolved Mass Spectrometry by : Pawel L. Urban

Download or read book Time-Resolved Mass Spectrometry written by Pawel L. Urban and published by John Wiley & Sons. This book was released on 2016-01-19 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches – introduced during the past few decades – have shown temporal resolutions ranging from hours down to microseconds and beyond. This text covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS – in the studies of reaction kinetics and mechanism, physicochemical phenomena, protein structure dynamics, biocatalysis, and metabolic profiling. It will assist science and engineering students to gain a basic understanding of the TRMS concept, and to recognize its usefulness. In addition, it may benefit scientists who conduct molecular studies in the areas of chemistry, physics and biology.

Modern Analytical Techniques

Author : Gunter Zweig
Publisher : Academic Press
ISBN 13 : 1483220915
Total Pages : 272 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Modern Analytical Techniques by : Gunter Zweig

Download or read book Modern Analytical Techniques written by Gunter Zweig and published by Academic Press. This book was released on 2013-10-22 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical Methods for Pesticides and Plant Growth Regulators, Volume XIV: Modern Analytical Techniques covers an updated treatment of the most frequently used techniques for pesticide analysis, i.e., thin-layer chromatography, gas chromatography (packed and capillary columns), high-performance liquid chromatography, and mass spectrometry. People involved in the analysis of pesticides will find the book useful.

Principles and Applications of Clinical Mass Spectrometry

Author : Nader Rifai
Publisher : Elsevier
ISBN 13 : 0128160640
Total Pages : 218 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Principles and Applications of Clinical Mass Spectrometry by : Nader Rifai

Download or read book Principles and Applications of Clinical Mass Spectrometry written by Nader Rifai and published by Elsevier. This book was released on 2018-06-26 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups. - Provides concrete examples of the main applications of mass spectrometry technology - Describes current capabilities of the LC- and MS-based analytical methods - Details methods for successful analytical work in the field

Selected Water Resources Abstracts

Author :
Publisher :
ISBN 13 :
Total Pages : 772 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Selected Water Resources Abstracts by :

Download or read book Selected Water Resources Abstracts written by and published by . This book was released on 1990 with total page 772 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Chemical and Biological Terrorism

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309061954
Total Pages : 303 pages
Book Rating : 4.3/5 (9 download)

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Book Synopsis Chemical and Biological Terrorism by : Institute of Medicine

Download or read book Chemical and Biological Terrorism written by Institute of Medicine and published by National Academies Press. This book was released on 1999-03-12 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: The threat of domestic terrorism today looms larger than ever. Bombings at the World Trade Center and Oklahoma City's Federal Building, as well as nerve gas attacks in Japan, have made it tragically obvious that American civilians must be ready for terrorist attacks. What do we need to know to help emergency and medical personnel prepare for these attacks? Chemical and Biological Terrorism identifies the R&D efforts needed to implement recommendations in key areas: pre-incident intelligence, detection and identification of chemical and biological agents, protective clothing and equipment, early recognition that a population has been covertly exposed to a pathogen, mass casualty decontamination and triage, use of vaccines and pharmaceuticals, and the psychological effects of terror. Specific objectives for computer software development are also identified. The book addresses the differences between a biological and chemical attack, the distinct challenges to the military and civilian medical communities, and other broader issues. This book will be of critical interest to anyone involved in civilian preparedness for terrorist attack: planners, administrators, responders, medical professionals, public health and emergency personnel, and technology designers and engineers.

Foodomics

Author : Alejandro Cifuentes
Publisher : John Wiley & Sons
ISBN 13 : 1118537351
Total Pages : 631 pages
Book Rating : 4.1/5 (185 download)

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Book Synopsis Foodomics by : Alejandro Cifuentes

Download or read book Foodomics written by Alejandro Cifuentes and published by John Wiley & Sons. This book was released on 2013-02-05 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the latest "-omics" tools to advance the study of food and nutrition The rapidly emerging field of foodomics examines food and nutrition by applying advanced "-omics" technologies in order to improve people's health, well-being, and knowledge. Using tools from genomics, transcriptomics, epigenomics, proteomics, and metabolomics, foodomics offers researchers new analytical approaches to solve a myriad of current challenges in food and nutrition science. This book presents the fundamentals of foodomics, exploring the use of advanced mass spectrometry techniques in food science and nutrition in the post-genomic era. The first chapter of the book offers an overview of foodomics principles and applications. Next, the book covers: Modern instruments and methods of proteomics, including the study and characterization of food quality, antioxidant food supplements, and food allergens Advanced mass spectrometry-based methods to study transgenic foods and the microbial metabolome Mass spectrometry-based metabolomics in nutrition and health research Foodomics' impact on our current understanding of micronutrients (phenolic compounds and folates), optimal nutrition, and personalized nutrition and diet related diseases Principles and practices of lipidomics and green foodomics Use of chemometrics in mass spectrometry and foodomics The final chapter of Foodomics explores the potential of systems biology approaches in food and nutrition research. All the chapters conclude with references to the primary literature, enabling readers to explore individual topics in greater depth. With contributions from a team of leading pioneers in foodomics, this book enables students and professionals in food science and nutrition to take advantage of the latest tools to advance their research and open up new areas of food and nutrition investigation.

Handbook of New Bacterial Systematics

Author : M. Goodfellow
Publisher :
ISBN 13 :
Total Pages : 584 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Handbook of New Bacterial Systematics by : M. Goodfellow

Download or read book Handbook of New Bacterial Systematics written by M. Goodfellow and published by . This book was released on 1993 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides microbiologists with a comprehensive treatment of concepts, ideas, and methods that make up the subject of modern bacterial systematics. It includes not only traditional numerical and chemotaxonomic methods, but also full coverage of molecular systematics. The book is divided into three sections - classification, nomenclature, and identification. The authors are world leaders in the field and many are involved in the "Bergey's manual," which is the bible of the field. Key Features * Provides microbiologists with a comprehensive treatment of the concepts ideas and methods that make up the subject of modern bacterial systematics. * Includes not only traditional numerical and chemotaxonomic methods but also full coverage of molecular systamatics. * Three sections included are: classification, nomenclature and identification. * The authors are world leaders in this subject - many are involved in the "Bergey's manual" which is the bible of the field.

Cell Analysis on Microfluidics

Author : Jin-Ming Lin
Publisher : Springer
ISBN 13 : 9811053944
Total Pages : 435 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Cell Analysis on Microfluidics by : Jin-Ming Lin

Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Mass Spectrometry in Chemical Biology

Author : Norberto Peporine Lopes
Publisher : Royal Society of Chemistry
ISBN 13 : 1788013468
Total Pages : 312 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Mass Spectrometry in Chemical Biology by : Norberto Peporine Lopes

Download or read book Mass Spectrometry in Chemical Biology written by Norberto Peporine Lopes and published by Royal Society of Chemistry. This book was released on 2017-11-16 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry is one of the most widespread technologies in chemistry and has been increasingly used in biology with the rise of omics sciences. This book summarizes some important methodological approaches in mass spectrometry and applications in the field of chemical biology. The core chapters build on basic concepts introduced in the opening chapter and explore established fields such as high throughput screening, proteomics and metabolomics. Emerging applications of mass spectrometry in elucidating biosynthetic pathways, enzyme mechanisms and protein-protein interactions are then presented. Connections between these diverse research fields are highlighted throughout. The book concludes with a discussion of databases and future perspectives. This book will be a useful tool to early chemical biology researchers wishing to incorporate mass spectrometry as a tool in their research.

Selected Water Resources Abstracts

Author :
Publisher :
ISBN 13 :
Total Pages : 1344 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Selected Water Resources Abstracts by :

Download or read book Selected Water Resources Abstracts written by and published by . This book was released on 1990 with total page 1344 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Rapid Antibody-based Technologies in Food Analysis

Author : Richard O’Kennedy
Publisher : Royal Society of Chemistry
ISBN 13 : 1788017919
Total Pages : 356 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Rapid Antibody-based Technologies in Food Analysis by : Richard O’Kennedy

Download or read book Rapid Antibody-based Technologies in Food Analysis written by Richard O’Kennedy and published by Royal Society of Chemistry. This book was released on 2019-05-29 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are significant challenges in food analysis, problems with food contamination and authentication, and a worldwide need to ensure food safety. This book provides a description of antibody-based technologies used in food analysis. It focuses on key applications, outlining the approaches used, their advantages and limitations, and describes future areas for development. Chapters are written by experts in the field, critically examining each of the currently used methodologies and highlighting new evolving technologies, such as lab-on-chip and microfluidics-based devices and biosensors. Case studies demonstrating the utility of each of the methods described are included. Important introductory chapters cover sample preparation for analysis and statistical sampling necessary for quality control for verification of results. An overview chapter highlighting major analytical issues and areas that have specific requirements, e.g. food authentication, is provided. Researchers and scientists in the field who have to acquire, verify and use technologies for food analysis, food producers and processors, food safety and testing laboratories, and government agencies will all find this a useful addition to their library.

Mass Spectrometry-Based Chemical Proteomics

Author : W. Andy Tao
Publisher : John Wiley & Sons
ISBN 13 : 1118970217
Total Pages : 449 pages
Book Rating : 4.1/5 (189 download)

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Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao

Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Industrial and Environmental Biotechnology

Author : Nuzhat Ahmed
Publisher : Horizon Press
ISBN 13 : 9781898486527
Total Pages : 0 pages
Book Rating : 4.4/5 (865 download)

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Book Synopsis Industrial and Environmental Biotechnology by : Nuzhat Ahmed

Download or read book Industrial and Environmental Biotechnology written by Nuzhat Ahmed and published by Horizon Press. This book was released on 2014-04-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contamination of the environment by herbicides, pesticides, solvents, various industrial byproducts (including toxic metals, radionucleotides and metalloids) is of enormous economic and environmental significance. Biotechnology can be used to develop "green" or environmentally friendly solutions to these problems by harnessing the ability of bacteria to adapt metabolic pathways, or recruit new genes to metabolise harmful compounds into harmless byproducts. In addition to its role in cleaning-up the environment, biotechnology can be used for the production of novel compounds with both agricultural and industrial applications. Internationally acclaimed authors from diverse fields present comprehensive reviews of all aspects of Industrial and Environmental Biotechnology. Chapters concerned with environmental biotechnology cover two major categories of pollutants: organic compounds and metals. Organic pollutants include cyclic aromatic compounds, with/without nitrogenous or chloride substitutions while metal pollutants include copper, chromate, silver, arsenic and mercury. The genetic basis of bioremediation and the microbial processes involved are examined, and the current and/or potential applications of bioremediation are discussed. The use of biotechnology for industrial and agricultural applications includes a chapter on the use of enzymes as biocatalysts to synthesize novel opiate derivatives of medical value. The conversion of low-value molasses to higher value products by biotechnological methods and the use tissue culture methods to improve sugar cane and potatoes crop production is discussed.

Heterotrophic Plate Counts and Drinking-water Safety

Author : Jamie Bartram
Publisher : IWA Publishing
ISBN 13 : 9781843390251
Total Pages : 278 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis Heterotrophic Plate Counts and Drinking-water Safety by : Jamie Bartram

Download or read book Heterotrophic Plate Counts and Drinking-water Safety written by Jamie Bartram and published by IWA Publishing. This book was released on 2003-08-31 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heterotrophic Plate Counts and Drinking-water Safety provides a critical assessment of the role of the Heterotrophic Plate Count (HPC) measurement in drinking water quality management. It was developed from an Expert workshop of 32 scientists convened by the World Health Organization and the WHO/NSF International Collaborating Centre for Drinking Water Safety and Treatment in Geneva, Switzerland. Heterotrophs are organisms, including bacteria, yeasts and moulds, that require an external source of organic carbon for growth. The HPC test (or Standard Plate Count), applied in many variants, is the internationally accepted test for measuring the hetrotrophic microorganism population in drinking water, and also other media. It measures only a fraction of the microorganisms actually present and does not distinguish between pathogens and non-pathogens. High levels of microbial growth can affect the taste and odor of drinking water and may indicate the presence of nutrients and biofilms which could harbor pathogens, as well as the possibility that some event has interfered with the normal production of the drinking water. HPC counts also routinely increase in water that has been treated by an in-line device such as a carbon filter or softener, in water-dispensing devices and in bottled waters and indeed in all water that has suitable nutrients, does not have a residual disinfectant, and is kept under sufficient conditions. There is debate among health professionals as to the need, utility or quantitative basis for health-based standards or guidelines relating to HPC-measured regrowth in drinking water. The issues that were addressed in this work include: the relationship between HPC in drinking water (including that derived from in-line treatment systems, dispensers and bottled water) and health risks for the general public the role of HPC as an indirect indicator or index for pathogens of concern in drinking water the role of HPC in assessing the efficacy and proper functioning of water treatment and supply processes the relationship between HPC and the aesthetic acceptability of drinking water. Heterotrophic Plate Counts and Drinking-water Safety provides valuable information on the utility and the limitations of HPC data in the management and operation of piped water systems as well as other means of providing drinking water to the public. It is of particular value to piped public water suppliers and bottled water suppliers, manufacturers and users of water treatment and transmission equipment and inline treatment devices, water engineers, sanitary and clinical microbiologists, and national and local public health officials and regulators of drinking water quality.