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Design Fabrication And Characterization Of Micro Nano Electroporation Devices For Drug Gene Delivery
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Book Synopsis Design, Fabrication and Characterization of Micro/nano Electroporation Devices for Drug/gene Delivery by : HyunChul Jung
Download or read book Design, Fabrication and Characterization of Micro/nano Electroporation Devices for Drug/gene Delivery written by HyunChul Jung and published by . This book was released on 2011 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: In current traditional electroporation methods, each cell experience different local process conditions due to non uniform electric fields, and high voltage. This leads to a degree of cell death and untransfected cells in every process. We designed and developed a novel micro/nanoscaled devices to apply relatively low and uniform electric field for well-controlled in vitro gene delivery and improved cell survival rate and overall transfection efficiency.
Book Synopsis Fabrication and Characterization of Membrane Electroporation Devices for Drug/ Gene Delivery by : Kun Yeh Chiang
Download or read book Fabrication and Characterization of Membrane Electroporation Devices for Drug/ Gene Delivery written by Kun Yeh Chiang and published by . This book was released on 2008 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Electroporation is a common technology for delivering genes and small or large molecules into cells. Local electroporation using a thin membrane with random micro- pores has shown significant enhancements over bulk electroporation on transfection efficiency and cell viability. In this study, we demonstrate a much less invasive and more efficient gene delivery method - membrane sandwich electroporation (MSE). We use a poly (e-caprolacton) (PCL) membrane with well defined micro-pore arrays for cell-trapping and gene delivery. Cells are immobilized on the micro-pore arrays by vacuum for localized electroporation. The localized electroporation is realized by applying voltage pulse or DC signal to achieve dielectric breakdown of the cell membrane. The conically circular pore arrays provide a great potential gradient when an electrical field is applied. This provides an efficient way to accelerate the rigid carriers to a high momentum for delivering species inside cells without damage to cell membrane. For device fabrication, the PCL membrane films are replicated from patterned SU-8 structures. The hollow microarrays in SU-8 with 10l̃5 um in height are transferred to a PDMS (Polydimethylsiloxane) film by soft lithography. The PDMS film with transferred circular pillar patterns is used as the mold for another replication of uniform PCL thin polymer films with a thickness of 5 to 8 um. NIH 3T3 cells are trapped and immobilized on micro-pore arrays by vacuum. Opti-MEM ITM reduced-serum medium is filled into the channels and the center reservoir before the DNA (pEGFP) sample is loaded into the inlet reservoir. Electroporation is carried out followed by a cell culture process for transfection efficiency characterization. The fluorescence images of NIH 3T3 cells after DNA delivery by electroporation in a MSE device with 5 [mu]m pores array indicate a uniform gene delivery. Evidently, the cells are uniformly trapped on the micro-pores array and each cell experiences the same condition during electroporation. Impedance measurements and device modeling are performed to study the behaviors at different biases and frequencies. DNA transfection with fluorescence expression by using probe station electroporation is able to be observed from 1 Volt to 40 Volt. Such devices for local electroporation with well defined micro-pore array structures have demonstrated great improvement in comparison with devices with random pores.
Book Synopsis Microfluidics and Bio-MEMS by : Tuhin S. Santra
Download or read book Microfluidics and Bio-MEMS written by Tuhin S. Santra and published by CRC Press. This book was released on 2020-11-01 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past two decades have seen rapid development of micro-/nanotechnologies with the integration of chemical engineering, biomedical engineering, chemistry, and life sciences to form bio-MEMS or lab-on-chip devices that help us perform cellular analysis in a complex micro-/nanoflluidic environment with minimum sample consumption and have potential biomedical applications. To date, few books have been published in this field, and researchers are unable to find specialized content. This book compiles cutting-edge research on cell manipulation, separation, and analysis using microfluidics and bio-MEMS devices. It illustrates the use of micro-robots for biomedical applications, vascularized microfluidic organs-on-a-chip and their applications, as well as DNA gene microarray biochips and their applications. In addition, it elaborates on neuronal cell activity in microfluidic compartments, microvasculature and microarray gene patterning, different physical methods for drug delivery and analysis, micro-/nanoparticle preparation and separation in a micro-/nanofluidic environment, and the potential biomedical applications of micro-/nanoparticles. This book can be used by academic researchers, especially those involved in biomicrofluidics and bio-MEMS, and undergraduate- and graduate-level students of bio-MEMS/bio-nanoelectromechanical systems (bio-NEMS), biomicrofluidics, biomicrofabricatios, micro-/nanofluidics, biophysics, single-cell analysis, bionanotechnology, drug delivery systems, and biomedical micro-/nanodevices. Readers can gain knowledge of different aspects of microfluidics and bio-MEMS devices; their design, fabrication, and integration; and biomedical applications. The book will also help biotechnology-based industries, where research and development is ongoing in cell-based analysis, diagnosis, and drug screening.
Book Synopsis Design, Fabrication, and Characterization of a Motion Stage for Scalable Imprinting of DNA Nanowires by : John Joseph LaColla
Download or read book Design, Fabrication, and Characterization of a Motion Stage for Scalable Imprinting of DNA Nanowires written by John Joseph LaColla and published by . This book was released on 2012 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis work examines the scalability of an imprinting stage utilizing parallel self-aligning mechanisms in a DNA combing and imprinting (DCl) process. Scalability is vital in developing efficient, low-cost and high-yield manufacturing processes, and improving the scalability of the DCI imprinting process will benefit biomedical research by enabling the affordable and scalable production of micro/nanoarrays for drug discovery, protein isolation, nanofluidics, and other applications. Previous work on the DCI process has primarily focused on the mechanics of the imprinting process rather than scale, and misalignments between the stamp and slide surfaces make it difficult to increase the scale without drastically increasing the complexity of the system, particularly when a 3 degree of freedom positioning device is used. Herein, a 1 degree of motion stage with 3 independent, passive self-aligning mechanisms is demonstrated to achieve high performance at 3 times the scale of previous devices. The influence of kinematic coupling repeatability, parallelism, and linear motion parasitics on the performance of the imprinting device was identified, and the device's performance was measured. The repeatability of the kinematic couplings and the magnitude of the parasitic motions were found to exceed the gage resolution of 12.7 [mu]m, and the initial parallelism variation of the stage is less than 140 [mu]m. A mathematical model to quantify the scalability of the device was also developed by examining its ability to handle misalignments in the stage, stamp, and slide alignment. Analysis with the model demonstrated the ability of the device to accommodate maximum misalignments ranging from 3.9° to 9.3°, confirming the minimal performance-scale tradeoff of a 1 degree of freedom motion stage. Through this analysis, this thesis demonstrates the effectiveness of parallel, self-aligning stamp mechanisms in a scalable DCI process, and provides a framework for future development of scalable imprinting stages.
Book Synopsis Fabrication and Characterization of a Micro Electroporation Cell Chip by : Fan Yang
Download or read book Fabrication and Characterization of a Micro Electroporation Cell Chip written by Fan Yang and published by . This book was released on 2004 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microarrays and Combinatorial Techniques : Design, Fabrication, and Analysis II by : Dan V. Nicolau
Download or read book Microarrays and Combinatorial Techniques : Design, Fabrication, and Analysis II written by Dan V. Nicolau and published by SPIE-International Society for Optical Engineering. This book was released on 2004 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of SPIE present the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields. Proceedings of SPIE are among the most cited references in patent literature.
Book Synopsis Micro/Nanofluidic Devices for Single Cell Analysis by : Fan-Gang Tseng
Download or read book Micro/Nanofluidic Devices for Single Cell Analysis written by Fan-Gang Tseng and published by MDPI. This book was released on 2018-10-04 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Micro/Nanofluidic Devices for Single Cell Analysis" that was published in Micromachines
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 994 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microfluidic Devices for Biomedical Applications by : Xiujun James Li
Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun James Li and published by Woodhead Publishing. This book was released on 2013-10-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries.
Book Synopsis Molecular Materials by : Sanjay Malhotra
Download or read book Molecular Materials written by Sanjay Malhotra and published by CRC Press. This book was released on 2017-05-12 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of molecular materials research looks at the preparation and characterization of potentially useful materials with enhanced physical, chemical, and biomedical properties. Molecular Materials: Preparation, Characterization, and Applications discusses the cutting-edge interdisciplinary research in the area of advanced molecular-based materials. This book explores multiple aspects of molecular materials, including their synthesis and characterization, and gives information on their application in various fields.
Book Synopsis Chitosan for Biomaterials IV by : R. Jayakumar
Download or read book Chitosan for Biomaterials IV written by R. Jayakumar and published by Springer Nature. This book was released on 2021-08-29 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the recent developments on the biomedical applications of chitosan and its derivatives. Chitosan exhibits unique properties such as non-toxicity, biodegradability and biocompatibility. Since its chemical structure and properties can be easily modified, it can be an ideal candidate as a biomaterial. Consequently, chitosan and its derivatives are being developed in different forms such as nanoparticles, micelles, nanofibers, hydrogels, films and 3D porous materials for various biomedical applications, ranging from drug and gene delivery to tissue engineering and regenerative medicine. The chapters of this volume focus on the potential use of chitosan and its derivatives as a hemostatic agent, tissue sealants, tissue engineering scaffolds, delivery carriers for bioactive molecules in bone tissue engineering and wound dressings. Some chapter’s deal with recent advancements of chitosan-based biomaterials as a drug, gene and transdermal drug delivery carrier. In addition, the volume focusses on the prospects of chitosan-based systems for the treatment of cancer, eye and other infectious diseases. The volume will be of interest to material scientists, chemists and biotechnologists by providing a better understanding of the physicochemical and biological characteristics of chitosan and its derivatives to develop more appropriate and innovative chitosan-based materials modified for unlimited practical applications in biomedical fields.
Book Synopsis Single Cell Analysis by : Tuhin Subhra Santra
Download or read book Single Cell Analysis written by Tuhin Subhra Santra and published by MDPI. This book was released on 2021-06-02 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Book Synopsis Microneedles for Drug and Vaccine Delivery and Patient Monitoring by : Ryan F. Donnelly
Download or read book Microneedles for Drug and Vaccine Delivery and Patient Monitoring written by Ryan F. Donnelly and published by John Wiley & Sons. This book was released on 2018-06-04 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides comprehensive coverage of microneedles for delivering and monitoring patient drugs and vaccines Microneedles are an incredibly active research area and have the potential to revolutionize the way many medicines and vaccines are delivered. This comprehensive research book covers the major aspects relating to the use of microneedle arrays in enhancing both transdermal and intradermal drug delivery and provides a sound background to the use of microneedle arrays in enhanced delivery applications. Beginning with a history of the field and the various methods employed to produce microneedles from different materials, Microneedles for Drug and Vaccine Delivery and Patient Monitoring discusses the penetration of the stratum corneum by microneedles and the importance of application method and force and microneedle geometry (height, shape, inter-needle spacing). Transdermal and intradermal delivery research using microneedles is comprehensively and critically reviewed, focusing on the outcomes of in vivo animal and human studies. The book describes the important topics of safety and patient acceptability studies carried out to date. It also covers in detail the growing area for microneedle use in the monitoring of interstitial fluid contents. Finally, it reviews translational and regulatory developments in the microneedles field and describes the work ongoing in industry. The only book currently available on microneedles Filled with tables, graphs, and black and white images (photographs, micrographs) Authored by four experts in pharmaceutics Microneedles for Drug and Vaccine Delivery and Patient Monitoring is an ideal source for researchers in industry and academia working on drug delivery and transdermal delivery in particular, as well as for advanced students in pharmacy and pharmaceutical sciences.
Book Synopsis Intracellular Delivery by : Aleš Prokop
Download or read book Intracellular Delivery written by Aleš Prokop and published by Springer Science & Business Media. This book was released on 2011-05-26 with total page 871 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.
Book Synopsis Degradable Aliphatic Polyesters by : A.-C. Albertsson
Download or read book Degradable Aliphatic Polyesters written by A.-C. Albertsson and published by Springer Science & Business Media. This book was released on 2001-12-03 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: A renewed interest in aliphatic polyesters has resulted in developing materials important in the biomedical and ecological fields. Mainly materials such as PLA and PCL homopolymers have so far been used in most applications. There are many other monomers which can be used. Different molecular structures give a wider range of physical properties as well as the possibility of regulating the degradation rate. By using different types of initiators and catalysts, ring-opening polymerization of lactones and lactides provides macromolecules with advanced molecular architectures. In the future, new degradable polymers should be able to participate in the metabolism of nature. Some examples of novel polymers with inherent environmentally favorable properties such as renewability and degradability and a series of interesting monomers found in the metabolisms and cycles of nature are given.
Book Synopsis Micro/Nano Devices for Blood Analysis by : Rui A. Lima
Download or read book Micro/Nano Devices for Blood Analysis written by Rui A. Lima and published by MDPI. This book was released on 2019-12-03 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of micro- and nanodevices for blood analysis is an interdisciplinary subject that demands the integration of several research fields, such as biotechnology, medicine, chemistry, informatics, optics, electronics, mechanics, and micro/nanotechnologies. Over the last few decades, there has been a notably fast development in the miniaturization of mechanical microdevices, later known as microelectromechanical systems (MEMS), which combine electrical and mechanical components at a microscale level. The integration of microflow and optical components in MEMS microdevices, as well as the development of micropumps and microvalves, have promoted the interest of several research fields dealing with fluid flow and transport phenomena happening in microscale devices. Microfluidic systems have many advantages over their macroscale counterparts, offering the ability to work with small sample volumes, providing good manipulation and control of samples, decreasing reaction times, and allowing parallel operations in one single step. As a consequence, microdevices offer great potential for the development of portable and point-of-care diagnostic devices, particularly for blood analysis. Moreover, the recent progress in nanotechnology has contributed to its increasing popularity, and has expanded the areas of application of microfluidic devices, including in the manipulation and analysis of flows on the scale of DNA, proteins, and nanoparticles (nanoflows). In this Special Issue, we invited contributions (original research papers, review articles, and brief communications) that focus on the latest advances and challenges in micro- and nanodevices for diagnostics and blood analysis, micro- and nanofluidics, technologies for flow visualization, MEMS, biochips, and lab-on-a-chip devices and their application to research and industry. We hope to provide an opportunity to the engineering and biomedical community to exchange knowledge and information and to bring together researchers who are interested in the general field of MEMS and micro/nanofluidics and, especially, in its applications to biomedical areas.
Book Synopsis Environmental, Chemical and Medical Sensors by : Shantanu Bhattacharya
Download or read book Environmental, Chemical and Medical Sensors written by Shantanu Bhattacharya and published by Springer. This book was released on 2017-12-22 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of sensor technologies as well as the recent research for the development of environmental, chemical and medical sensor technologies. Chapters include current research on microflow cytometry, microfluidic devices, colorimetric sensors, and the development of low-cost optical densitometric sensors and paper based analytical devices for environmental and biomedical applications. Special focus has been given to nanotechnology and nanostructures- their fabrication, uses and utility in different fields of research such as for the design of tools for medical diagnostics, therapeutics, as well as for detection and estimation of pollutant levels in water and air quality monitoring. This book is intended as a resource for researchers working in the field of sensor development across the world.
