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Author : Ramon Kenneth Tabtiang
Publisher :
ISBN 13 :
Total Pages : 380 pages
Book Rating : 4.:/5 (66 download)
Download or read book Combinatorial Regulation of HO Transcription in Saccharomyces Cerevisiae written by Ramon Kenneth Tabtiang and published by . This book was released on 1998 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Thomas William Engar
Publisher :
ISBN 13 :
Total Pages : 74 pages
Book Rating : 4.:/5 (693 download)
Download or read book Regulation of Gene Expression in Saccharomyces Cerevisiae written by Thomas William Engar and published by . This book was released on 2006 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Umut Eser
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (83 download)
Download or read book Spatio Temporal Regulation of Cell Cycle Transcription written by Umut Eser and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The Start checkpoint regulates cell cycle commitment and associated transcription in the budding yeast, Saccharomyces cerevisiae. It was previously shown that commitment to cell division corresponds to activating the positive feedback loop of G1 cyclins controlled by the transcription factors SBF and MBF. Around this pivotal cell cycle event, over 300 genes (G1/S regulon) are expressed to facilitate the G1/S transition. Despite its importance, little was known about distinct temporal regulation within the G1/S regulon. We found that SBF and MBF target genes have a well-defined distribution of transcriptional activation times. We also showed that activation of G1 cyclins precedes the activation of the bulk of the G1/S regulon, which we named 'feedback-first' regulation. In budding yeast, feedback-first regulation ensures that commitment to cell division occurs before large-scale changes in transcription. Thus, the transition can be viewed as a two-step process whereby the decision to divide precedes synthesis of the cellular machinery required for division. Furthermore, we found that feedback-first regulation is conserved in the related yeast S. bayanus as well as human cells. This finding highlighted the importance of understanding the molecular mechanisms through which co-regulated genes can have distinct activation dynamics. We showed that timing is partially explained by the combinatorial use of SBF and MBF transcription factors, which implement a logical OR function for gene activation. In addition to combinatorial use of transcription factors, we analyzed genome-wide chromosome conformation capture data to examine the potential link between the timing of gene expression and 3-D genome architecture. The early-activated genes of the G1/S regulon are significantly enriched for the number of physical contacts to the rest of the genome. Further analysis revealed two main clusters, whose interactions co-vary and whose activation time distributions are distinct. Taken together, these our work explains a significant amount of timing variation within cell cycle-dependent gene expression. Thus, we concluded that the cell utilizes both genome architecture and the combinatorial use of transcription factors to implement feedback-first regulation ensuring that commitment to cell division precedes genome-wide cell cycle-dependent transcription.
Author : Ulrich Kück
Publisher : Springer Science & Business Media
ISBN 13 : 3662103648
Total Pages : 372 pages
Book Rating : 4.6/5 (621 download)
Download or read book Genetics and Biotechnology written by Ulrich Kück and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mycology, the study of fungi, originated as a subdiscipline of botany and was a descriptive discipline, largely neglected as an experimental science until the early years of this century. A seminal paper by Blakeslee in 1904 provided evidence for self incompatibility, termed "heterothallism", and stimulated interest in studies related to the control of sexual reproduction in fungi by mating-type specificities. Soon to follow was the demonstration that sexually reproducing fungi exhibit Mendelian inheritance and that it was possible to conduct formal genetic analysis with fungi. The names Burgeff, Kniep and Lindegren are all associated with this early period of fungal genetics research. These studies and the discovery of penicillin by Fleming, who shared a Nobel Prize in 1945, provided further impetus for experimental research with fungi. Thus began a period of interest in mutation induction and analysis of mutants for bio chemical traits. Such fundamental research, conducted largely with Neurospora crassa, led to the one gene: one enzyme hypothesis and to a second Nobel Prize for fungal research awarded to Beadle and Tatum in 1958. Fundamental research in biochemical genetics was extended to other fungi, especially to Saccharomyces cere visiae, and by the mid-1960s fungal systems were much favored for studies in eukaryotic molecular biology and were soon able to compete with bacterial systems in the molecular arena.
Author : Josephine Ho
Publisher :
ISBN 13 :
Total Pages : 162 pages
Book Rating : 4.:/5 (12 download)
Download or read book Investigating the Regulatory Relationships Between Transcription Factors and Their Targets in Saccharomyces Cerevisiae Using RNA-seq written by Josephine Ho and published by . This book was released on 2017 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Nicholas Santoro
Publisher :
ISBN 13 :
Total Pages : 478 pages
Book Rating : 4.3/5 (91 download)
Download or read book Distinct Binding Sites and Signaling Pathways Regulate the Saccharomyces Cerevisiae Heat Shock Transcription Factor written by Nicholas Santoro and published by . This book was released on 2000 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Megan Denise Lenardon
Publisher :
ISBN 13 :
Total Pages : 340 pages
Book Rating : 4.:/5 (224 download)
Download or read book The Role of Auxiliary Transcription Factors in the Regulation of Gene Expression During Sporulation in Saccharomyces Cerevisiae written by Megan Denise Lenardon and published by . This book was released on 2005 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Barrett C. Foat
Publisher :
ISBN 13 : 9780549054207
Total Pages : 87 pages
Book Rating : 4.0/5 (542 download)
Download or read book Quantitative Modeling of Gene Expression Regulation by RNA-binding Proteins and Transcription Factors in Saccharomyces Cerevisiae written by Barrett C. Foat and published by . This book was released on 2007 with total page 87 pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of gene expression is one of the means by which cells modulate their behavior. Moreover, the steady-state concentration of an mRNA transcript is the result of both the rate of transcription and the rate of mRNA decay. Cells regulate transcription and mRNA decay via nucleic acid binding proteins that physically interact with their DNA or RNA targets. Genome-scale technologies such as mRNA expression microarrays and chromatin immunoprecipitation microarrays generate data that reflect the activities of these transcription and decay factors. By interpreting these genomics data through statistical-mechanical models for regulator-nucleic acid interactions, we can discover binding sites for known and unknown regulators and characterize both their sequence-specific binding affinities and their condition-specific regulatory activities. This process is embodied in an algorithm I developed called MatrixREDUCE.
Author : Vasudha Bharatula
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (111 download)
Download or read book Regulation of Stress Induced Gene Expression in Saccharomyces Cerevisiae written by Vasudha Bharatula and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription initiation is complex process involving transcription factors(TF), co-activators, nucleosome remodelers and the pre-initiation complex (PIC);general transcription factors (GTF) and RNA polymerase II (RNAP2). The spatiotemporalorganization of these different proteins and their role in regulatingplasticity and selectivity of transcriptional reprogramming in various stressesremains unknown. I describe the role of Multicopy Suppressor of Snf1 mutation(Msn2); a stress responsive transcription factor, Mediator (coactivator) andnucleosome remodeling in fine-tuning gene expression in response to stress inyeast.Msn2 exhibits distinct patterns of nucleo-cytoplasmic oscillations indifferent stresses. I determined that Msn2 target genes exhibited fast or slowinduction kinetics in response to transient and persistent Msn2 nuclearoccupancy respectively. Chromatin immunoprecipitation studies revealed thatMsn2 binds promoters of common stress genes as well as condition specificgenes in nutrient and oxidative stresses, suggesting that TF dynamics could playa role in selective gene regulation in different stresses. Additionally, Msn2binding to ~30 oxidative genes was dependent on Yap1. However, deleting Msn2did not lead to a significant decrease in expression of oxidative stress responsegenes as did the absence of Yap1. These results suggest that indirectcooperativity between Msn2 and Yap1 could lead to selective promoter bindingbut the effects of such interactions on gene expression remain unclear.I studied the role of Mediator in native conditions, where its function has not been adequately addressed. Mediator occupies chromosomal interacting domains (CID), which mark boundaries between interacting genomic regions, suggesting that Mediator could play a significant role in higher-order genome organization along with functioning as a coactivator.Finally, I assessed the combined effect of Msn2, Mediator and nucleosome remodeling on gene expression upon nutrient deprivation. Both Msn2 and Mediator are selectively recruited to promoters of genes activated and repressed in nutrient stress. Genes activated by Msn2 exhibited rapid loss of nucleosomes, in an Msn2 dependent manner. In contrast, repression of genes was not accompanied by a significant gain in nucleosomes, suggesting additional mechanisms of repression could exist. The absence of Msn2, significantly decreased Mediator recruitment at promoters of target genes, indicating that Mediator- TF interactions are crucial for gene expression changes in stress. Interestingly, both RNAP2 and Mediator remained poised at promoters of repressed genes, possibly to re-initiate transcription once the stress has passed.These findings offer novel insights on how Msn2 regulates expression of specific genes depending on the environment by modulating its dynamics and genomic binding. The binding of Msn2 helps recruit Mediator and facilitate nucleosome loss at genes induced in stress. Whereas, repressed genes seems to possess Mediator and RNAP2 in an inactive form which could be the mechanism of repression and a way to activate these genes immediate after stress.
Author : Brigitte Ganter Seghezzi
Publisher :
ISBN 13 :
Total Pages : 330 pages
Book Rating : 4.:/5 (312 download)
Download or read book Regulation of Transcription of Mating-type Specific Genes in the Yeast Saccharomyces Cerevisiae written by Brigitte Ganter Seghezzi and published by . This book was released on 1994 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Laura Lynn Newcomb
Publisher :
ISBN 13 :
Total Pages : 144 pages
Book Rating : 4.:/5 (89 download)
Download or read book Connections Between Growth and Division written by Laura Lynn Newcomb and published by . This book was released on 2001 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Naoko Kobayashi
Publisher :
ISBN 13 :
Total Pages : 422 pages
Book Rating : 4.:/5 ( download)
Download or read book Transcriptional Regulation of a Stress Inducible Gene of Saccharomyces Cerevisiae written by Naoko Kobayashi and published by . This book was released on 1990 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Xu Zhou
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (864 download)
Download or read book Mechanisms of Transcriptional Control in Phosphate-responsive Signaling Pathway of Saccharomyces Cerevisiae written by Xu Zhou and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Regulation of gene expression is essential for many biological processes. Binding of transcription factors to DNA is a key regulatory step in the control of gene expression. It is commonly observed that DNA sequences with high affinity for transcription factors occur more frequently in the genome than the instances of genes bound or regulated by these factors. However, the mechanism by which transcription factors selectively identify and regulate these genes was unclear. I utilized the transcriptional control of the phosphate-responsive signaling pathway (PHO) in Saccharomyces cerevisiae as a model system to address this problem.
Author : Shelley Chu
Publisher :
ISBN 13 :
Total Pages : 470 pages
Book Rating : 4.:/5 (66 download)
Download or read book Transcriptional Regulation of Meiosis and Spore Morphogenesis in Saccharomyces Cerevisiae written by Shelley Chu and published by . This book was released on 1998 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Priyasri Shotton
Publisher :
ISBN 13 :
Total Pages : 268 pages
Book Rating : 4.:/5 (828 download)
Download or read book A Study of the Interplay Between Saccharomyces Cerevisiae Transcription and DNA Repair written by Priyasri Shotton and published by . This book was released on 2012 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA is the storehouse of genetic information for the normal functioning of all cellular processes. This DNA is "read" by RNA Polymerases (RNAP) to carry out the transcription process, which is one of the most vital regulatory mechanisms within the cell for gene expression. There are three important RNA Polymerases available in the cell. RNAPI synthesizes ribosomal RNAs, while RNAPII makes mRNA precursors, snRNAs and most microRNAs, additionally RNAPIII takes care of tRNAs, small RNAs and 5S rRNAs that occur in the nucleus and the cytosol. The work in the author's lab focuses on the function of RNAPII transcription. It is not straightforward for RNAPII to transcribe the DNA, it needs to struggle with two major roadblocks that are in its path. First, is the nucleosomal barrier that is scattered across the coding regions of active genes. The mechanism to overcome this major obstacle is a very complex process that involves an extensive range of proteins which work collectively to help in the alteration of chromatin structure that ultimately facilitates transcriptional regulation. In the thesis, the author has attempted to take a look at this intricate network and has studied the function of Rad26p, in yeast (associated with CSB-Cockayne syndrome B, in humans), a transcription coupled DNA repair factor, having an additional role in transcription. The results reveal that Rad26p plays a novel role in the regulation of elongation by modulating histone H2A-H2B dimer occupancy.
Author : Christopher T. Harbison
Publisher :
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.:/5 (65 download)
Download or read book Genome-wide Analysis of Transcriptional Expression Programs, Regulatory Networks and Cis-regulatory Sequences in Saccharomyces Cerevisiae written by Christopher T. Harbison and published by . This book was released on 2005 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Historically, knowledge of gene-specific transcription has been accumulated by the study of the individual genetic and physical interactions between transcriptional regulators and the genes they regulate, often requiring considerable time and effort. Microarray technology now enables investigation of gene expression at the level of the entire genome, allowing researchers access to rich datasets and promising new levels of depth in the understanding of transcriptional regulation. Our lab has made use of these technologies both to measure the levels of all mRNA transcripts within a population of cells, as well as to locate the regions within the genome that are bound by transcriptional regulators. Such studies not only allow for the functional annotation of both genes and regulators, but can also provide clues about the identity of the regulatory regions within DNA, the structure of global regulatory networks and the regulation of DNA-binding proteins. These and other insights are presented here based on our genome-wide studies of transcriptional regulation in the yeast Saccharomyces cerevisiae.
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Author : Kate Chabarek
Publisher :
ISBN 13 :
Total Pages : 98 pages
Book Rating : 4.:/5 (73 download)
Download or read book Regulation of Copper-binding Transcription Factor MAC[1] by PHO85 in the Yeast Saccharomyces Cerevisiae written by Kate Chabarek and published by . This book was released on 2006 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:
