Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

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Publisher : Academic Press
ISBN 13 : 0128201487
Total Pages : 360 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems by :

Download or read book Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems written by and published by Academic Press. This book was released on 2020-05-14 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on retinoid signaling pathways

Advanced Chemical Biology

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Publisher : John Wiley & Sons
ISBN 13 : 352734733X
Total Pages : 806 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Advanced Chemical Biology by : Howard C. Hang

Download or read book Advanced Chemical Biology written by Howard C. Hang and published by John Wiley & Sons. This book was released on 2023-04-03 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Chemical Biology The modern approach to teaching chemical biology Advanced Chemical Biology is organized around the central dogma of life, progressing from genes to proteins and higher-order cellular structures, including core application areas such as imaging, chemical genetics, activity-based protein profiling, and natural product discovery and biosynthesis. Advanced topics and applications in, e. g., microbiology, developmental biology, and neurobiology, are covered in separate sections. Every chapter is homogeneous in style and layout, consisting of a short historical introduction followed by a description of the underlying concepts and a selection of recent examples of how the concept has been turned into practice. The subdivision of the contents into core and supplemental chapters enables a flexible use in teaching, both for a one-semester and a two-semester course. Written by authors and editors coming from the leading scientific institutions that have developed the concepts and technologies for this discipline, Advanced Chemical Biology includes specific information on topics like: DNA function, synthesis and engineering, chemical approaches to genome integrity, and RNA function, synthesis, and probing Chemical approaches to transcription and RNA regulation in vivo, chemical biology of genome engineering, and peptide/protein synthesis and engineering Directed evolution for chemical biology, chemical biology of cellular metabolism, chemical biology of lipids, and protein post-translational modifications Chemical glycobiology, chemical and enzymatic modification of proteins, genetic code expansion, bio-orthogonal chemistry, and cellular imaging With its broad scope and focus on turning concepts into applications, Advanced Chemical Biology is an excellent starting point for anyone entering the field and looking for a guide to the wide range of available methods and strategies that chemical biology has to offer. With a Foreword by Nobel Laureate Carolyn Bertozzi.

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

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Author :
Publisher : Academic Press
ISBN 13 : 0128211555
Total Pages : 528 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods by :

Download or read book Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods written by and published by Academic Press. This book was released on 2020-07-24 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing ß-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on retinoid signaling pathways

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

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Author :
Publisher : Academic Press
ISBN 13 : 0128211520
Total Pages : 432 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation by :

Download or read book Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation written by and published by Academic Press. This book was released on 2020-05-29 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on retinoid signaling pathways

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence

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Author :
Publisher : Academic Press
ISBN 13 : 0128211539
Total Pages : 342 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence by :

Download or read book Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence written by and published by Academic Press. This book was released on 2020-06-30 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on retinoid signaling pathways

Molecular Biology of the Cell

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Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Science and Applications of Synthetic and Systems Biology

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Publisher : National Academies Press
ISBN 13 : 0309219396
Total Pages : 570 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis The Science and Applications of Synthetic and Systems Biology by : Institute of Medicine

Download or read book The Science and Applications of Synthetic and Systems Biology written by Institute of Medicine and published by National Academies Press. This book was released on 2011-12-30 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.

Metal Nanoparticles in Microbiology

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Publisher : Springer Science & Business Media
ISBN 13 : 3642183123
Total Pages : 306 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Metal Nanoparticles in Microbiology by : Mahendra Rai

Download or read book Metal Nanoparticles in Microbiology written by Mahendra Rai and published by Springer Science & Business Media. This book was released on 2011-04-02 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following an introduction to biogenic metal nanoparticles, this book presents how they can be biosynthesized using bacteria, fungi and yeast, as well as their potential applications in biomedicine. It is shown that the synthesis of nanoparticles using microbes is eco-friendly and results in reproducible metal nanoparticles of well-defined sizes, shapes and structures. This biotechnological approach based on the process of biomineralization exploits the effectiveness and flexibility of biological systems. Chapters include practical protocols for microbial synthesis of nanoparticles and microbial screening methods for isolating a specific nanoparticle producer as well as reviews on process optimization, industrial scale production, biomolecule-nanoparticle interactions, magnetosomes, silver nanoparticles and their numerous applications in medicine, and the application of gold nanoparticles in developing sensitive biosensors.

Inspired by Biology

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Publisher : National Academies Press
ISBN 13 : 0309134293
Total Pages : 170 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Inspired by Biology by : National Research Council

Download or read book Inspired by Biology written by National Research Council and published by National Academies Press. This book was released on 2008-06-17 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists have long desired to create synthetic systems that function with the precision and efficiency of biological systems. Using new techniques, researchers are now uncovering principles that could allow the creation of synthetic materials that can perform tasks as precise as biological systems. To assess the current work and future promise of the biology-materials science intersection, the Department of Energy and the National Science Foundation asked the NRC to identify the most compelling questions and opportunities at this interface, suggest strategies to address them, and consider connections with national priorities such as healthcare and economic growth. This book presents a discussion of principles governing biomaterial design, a description of advanced materials for selected functions such as energy and national security, an assessment of biomolecular materials research tools, and an examination of infrastructure and resources for bridging biological and materials science.

Expansion Microscopy for Cell Biology

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Publisher : Academic Press
ISBN 13 : 0128208082
Total Pages : 358 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Expansion Microscopy for Cell Biology by :

Download or read book Expansion Microscopy for Cell Biology written by and published by Academic Press. This book was released on 2021-01-19 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Represents the latest release in the Methods in Cell Biology series - Includes the latest information on Expansion Microscopy for Cell Biology

Quantitative Imaging in Cell Biology

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Publisher : Academic Press
ISBN 13 : 0124202012
Total Pages : 609 pages
Book Rating : 4.1/5 (242 download)

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Book Synopsis Quantitative Imaging in Cell Biology by :

Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

The Social Biology of Microbial Communities

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Publisher : National Academies Press
ISBN 13 : 0309264324
Total Pages : 633 pages
Book Rating : 4.3/5 (92 download)

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Book Synopsis The Social Biology of Microbial Communities by : Institute of Medicine

Download or read book The Social Biology of Microbial Communities written by Institute of Medicine and published by National Academies Press. This book was released on 2013-01-10 with total page 633 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the germ theory of disease in the 19th century and extending through most of the 20th century, microbes were believed to live their lives as solitary, unicellular, disease-causing organisms . This perception stemmed from the focus of most investigators on organisms that could be grown in the laboratory as cellular monocultures, often dispersed in liquid, and under ambient conditions of temperature, lighting, and humidity. Most such inquiries were designed to identify microbial pathogens by satisfying Koch's postulates.3 This pathogen-centric approach to the study of microorganisms produced a metaphorical "war" against these microbial invaders waged with antibiotic therapies, while simultaneously obscuring the dynamic relationships that exist among and between host organisms and their associated microorganisms-only a tiny fraction of which act as pathogens. Despite their obvious importance, very little is actually known about the processes and factors that influence the assembly, function, and stability of microbial communities. Gaining this knowledge will require a seismic shift away from the study of individual microbes in isolation to inquiries into the nature of diverse and often complex microbial communities, the forces that shape them, and their relationships with other communities and organisms, including their multicellular hosts. On March 6 and 7, 2012, the Institute of Medicine's (IOM's) Forum on Microbial Threats hosted a public workshop to explore the emerging science of the "social biology" of microbial communities. Workshop presentations and discussions embraced a wide spectrum of topics, experimental systems, and theoretical perspectives representative of the current, multifaceted exploration of the microbial frontier. Participants discussed ecological, evolutionary, and genetic factors contributing to the assembly, function, and stability of microbial communities; how microbial communities adapt and respond to environmental stimuli; theoretical and experimental approaches to advance this nascent field; and potential applications of knowledge gained from the study of microbial communities for the improvement of human, animal, plant, and ecosystem health and toward a deeper understanding of microbial diversity and evolution. The Social Biology of Microbial Communities: Workshop Summary further explains the happenings of the workshop.

Real-time Coastal Observing Systems for Marine Ecosystem Dynamics and Harmful Algal Blooms

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Publisher : UNESCO
ISBN 13 : 9231040421
Total Pages : 880 pages
Book Rating : 4.2/5 (31 download)

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Book Synopsis Real-time Coastal Observing Systems for Marine Ecosystem Dynamics and Harmful Algal Blooms by : Babin, Marcel

Download or read book Real-time Coastal Observing Systems for Marine Ecosystem Dynamics and Harmful Algal Blooms written by Babin, Marcel and published by UNESCO. This book was released on 2008-06-05 with total page 880 pages. Available in PDF, EPUB and Kindle. Book excerpt: The proliferation of harmful phytoplankton in marine ecosystems can cause massive fish kills, contaminate seafood with toxins, impact local and regional economies and dramatically affect ecological balance. Real-time observations are essential for effective short-term operational forecasting, but observation and modelling systems are still being developed. This volume provides guidance for developing real-time and near real-time sensing systems for observing and predicting plankton dynamics, including harmful algal blooms, in coastal waters. The underlying theory is explained and current trends in research and monitoring are discussed.Topics covered include: coastal ecosystems and dynamics of harmful algal blooms; theory and practical applications of in situ and remotely sensed optical detection of microalgal distributions and composition; theory and practical applications of in situ biological and chemical sensors for targeted species and toxin detection; integrated observing systems and platforms for detection; diagnostic and predictive modelling of ecosystems and harmful algal blooms, including data assimilation techniques; observational needs for the public and government; and future directions for research and operations.

Current Protocols Essential Laboratory Techniques

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Publisher : John Wiley & Sons
ISBN 13 : 047094241X
Total Pages : 679 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Current Protocols Essential Laboratory Techniques by : Sean R. Gallagher

Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Correlative Light and Electron Microscopy IV

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Publisher : Academic Press
ISBN 13 : 0128220597
Total Pages : 452 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Correlative Light and Electron Microscopy IV by :

Download or read book Correlative Light and Electron Microscopy IV written by and published by Academic Press. This book was released on 2021-03-09 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. - Contains contributions from experts in the field - Covers topics using nano-SIMS and EDX for CLEM - Presents recent advances and currently applied correlative approaches - Gives detailed protocols, allowing for the application of workflows in one's own laboratory setting - Covers CLEM approaches in the context of specific applications - Aims to stimulate the use of new combinations of imaging modalities

Introductory Experiments on Biomolecules and their Interactions

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Publisher : Academic Press
ISBN 13 : 0128010126
Total Pages : 123 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Introductory Experiments on Biomolecules and their Interactions by : Robert K. Delong

Download or read book Introductory Experiments on Biomolecules and their Interactions written by Robert K. Delong and published by Academic Press. This book was released on 2015-03-06 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author's experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class. - Features quantitative analysis of RNA degradation by RNase - Contains problem sets, calculations, and references for each lab fully immersing students in the learning process - Includes instruction on how to maintain a lab notebook and write a formal lab report - Provides hands-on engagement with the four major types of biomolecules and "real-life and better applied examples of molecular interactions

Enzymes of Epigenetics Part B

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Publisher : Elsevier
ISBN 13 : 0128119020
Total Pages : 466 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Enzymes of Epigenetics Part B by :

Download or read book Enzymes of Epigenetics Part B written by and published by Elsevier. This book was released on 2016-07-14 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes of Epigenetics: Part B, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods that are employed in the study of epigenetic regulation, including structural, biochemical, molecular, biological, cellular, computational, and systems approaches. Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies, and small molecule epigenetic regulators and biological connections. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Contains two new volumes that cover research methods in enzymes of epigenetics - Covers such topics as chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections