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Chemical And Physical Properties Of Bovine Alpha Lactalbumin Beta Casein And Genetically Altered Bovine Beta Casein Produced In The Milk Of Transgenic Mice
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Book Synopsis Chemical and Physical Properties of Bovine Alpha-lactalbumin, Beta- Casein and Genetically Altered Bovine Beta-casein Produced in the Milk of Transgenic Mice by : Shin-Yi Jeng
Download or read book Chemical and Physical Properties of Bovine Alpha-lactalbumin, Beta- Casein and Genetically Altered Bovine Beta-casein Produced in the Milk of Transgenic Mice written by Shin-Yi Jeng and published by . This book was released on 1996 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: The genomic bovine $alpha$-lactalbumin gene (1.7 kb) and the bovine $beta$-casein gene (8.5 kb) linked to bovine $alpha$-lactalbumin 5$prime$ flanking region (2.0 kb) were expressed in two lines of transgenic mice, $alpha$-114 and $alphabeta$ 34, respectively. Whey proteins, caseins, or skim milk proteins of the transgenic mouse milk containing bovine $alpha$-lactalbumin or bovine $beta$-casein were analyzed and separated by polyacrylamide gel electrophoresis (PAGE), using SDS, urea, or native (non-denaturing) conditions. Using PAGE followed by immunoblotting, both heterologous proteins were proved as identical as the native proteins in the molecular weight and the charge. The estimated expression level of bovine $alpha$-lactalbumin and $beta$-casein was about 1.0 mg/ml and 3.0 mg/ml, respectively. The expression level of bovine $alpha$-lactalbumin was lactation-dependent, which was higher in early lactation than that in late lactation stage. Calcium binding of the heterologous bovine $alpha$-lactalbumin was also the same as native bovine $alpha$-lactalbumin. The phosphorylation level of the bovine $beta$-casein expressed in the transgenic mouse milk was identical to the native bovine $beta$-casein. The glycosylated bovine $alpha$-lactalbumin and the N-terminal amino acid sequence of purified bovine $alpha$-lactalbumin were characterized and determined. The physical properties of transgenic mouse milks were changed due to the heterologous expression of the bovine $beta$-casein or $alpha$-lactalbumin in the mouse milks. An increased moisture of transgenic mouse milk containing bovine $alpha$-lactalbumin was observed. However, the milk composition of transgenic mouse milk containing bovine $beta$-casein did not change significantly. In addition, two chymosin-resistant and one plasmin-resistant bovine $beta$-casein genomic constructs were generated and microinjected to generate transgenic mice. Mouse milk containing each of the three mutant $beta$-caseins was produced and analyzed by immunoblotting with a bovine $beta$-casein specific antibody. The mice carrying one of the chymosin-resistant gene constructs produced the milk containing mutant bovine $beta$-casein with the expected molecular weight. The result of rennin digestion demonstrated that the peptide bond of 192-193 in the mutant protein was chymosin-resistant. However, the mice carrying the plasmin-resistant $beta$-casein produced two proteins in their milk which reacted with bovine $beta$-casein antibody and had smaller molecular weights than that of the expected mutant $beta$-casein.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1997 with total page 826 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Chemical and Physical Properties of Alpha and Beta Casein by : Elroy Carl Hagberg
Download or read book Chemical and Physical Properties of Alpha and Beta Casein written by Elroy Carl Hagberg and published by . This book was released on 1949 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Modification of Bovine Beta-casein Using Genetic Engineering and Its Structure/function Studies by : Byung-Kwon Choi
Download or read book Modification of Bovine Beta-casein Using Genetic Engineering and Its Structure/function Studies written by Byung-Kwon Choi and published by . This book was released on 1996 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The putative glycosylation site (Asn-X-Ser) was introduced to the bovine $beta$-casein cDNA and genomic DNA (A$sp2$ genetic variant) using PCR-based site-directed mutagenesis to increase its amphiphilicity. The two mutant bovine $beta$-casein sequences containing the substitution of ${bf Ser}sb{70}$ for ${bf Leu}sb{70}$ (Asn$sb{68}$-Ser$sb{69}$-${bf Ser}sb{70}$-Pro$sb{71})$ and the substitution of ${bf Ser}sb{71}$ for ${bf Pro}sb{71}$ (Asn$sb68$-Ser$sb{69}$-${bf Ser}sb{70}$-${bf Ser}sb{71})$ were constructed and expressed using Pichia pastoris and transgenic mice. The mutant constructs were placed under the control of the methanol-inducible alcohol oxidase (AOX1) promoter for P. pastoris and of the bovine $alpha$-lactalbumin promoter for transgenic mice. The N-linked glycosylation of the mutant carrying Pro$sb{71}$ was completely inhibited whereas the other mutant carrying Ser$sb{71}$ was N-linked glycosylated in both expression systems. Thus, the C-terminal proline residue in the sequon was found to be a structural inhibitor for N-glycosylation. Glyco-$beta$-caseins produced in both systems were phosphorylated to the same degree as authentic bovine $beta$-casein. In addition, based on lectin blots, the oligosaccharides of glyco-$beta$-casein from P. pastoris were high-mannose type whereas transgenic glyco-$beta$-caseins contained various oligosaccharide chains. Mouse casein micelles containing glycosylated bovine $beta$-casein showed the largest median diameter and rough outer surfaces, compared to normal mouse casein micelles and micelles from transgenic milk containing wild-type bovine $beta$-casein.
Book Synopsis Animal Biotechnology by : National Research Council
Download or read book Animal Biotechnology written by National Research Council and published by National Academies Press. This book was released on 2002-12-29 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.
Author :University of Illinois at Urbana-Champaign. Agricultural Experiment Station Publisher : ISBN 13 : Total Pages :196 pages Book Rating :4.:/5 (31 download)
Book Synopsis Recent Progress in Solving Some Farm Problems of Illinois by : University of Illinois at Urbana-Champaign. Agricultural Experiment Station
Download or read book Recent Progress in Solving Some Farm Problems of Illinois written by University of Illinois at Urbana-Champaign. Agricultural Experiment Station and published by . This book was released on 1996 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development of Enzyme Linked Immunospecific Assay of Casien and Periparturient Changes of Bovine Alpha-lactalbumin and Alpha-, Beta-casein by : Heng-Cherl Yom
Download or read book Development of Enzyme Linked Immunospecific Assay of Casien and Periparturient Changes of Bovine Alpha-lactalbumin and Alpha-, Beta-casein written by Heng-Cherl Yom and published by . This book was released on 1986 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1996 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Investigation on Beta Casein and Alpha Lactalbumin Genes and Their Association with Milk Yield in Tharparkar Breed of Cattle by : S. Pathak
Download or read book Investigation on Beta Casein and Alpha Lactalbumin Genes and Their Association with Milk Yield in Tharparkar Breed of Cattle written by S. Pathak and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1988 with total page 1284 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Milk Proteins written by Isabel Gigli and published by BoD – Books on Demand. This book was released on 2016-09-07 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Milk proteins have nutritional value and extraordinary biological properties. Research over the last decades has provided new insight into the structure and the function of milk bioactive peptides. Some of these peptides are delivered directly into milk, and some are encrypted in major proteins such as caseins and lactoglobulins. These peptides have antimicrobial functions modulating the gut microflora. Even when milk is undisputedly the first food for mammals, milk proteins sometimes can be a health threat, either because of allergic reaction or because of toxicity. In this regard, in vitro studies showed donkey's casein and major whey proteins to be more digestible than cows' for human consumption. In this book, readers will find updated research on the major milk proteins' structure, bioactive peptides, milk protein allergy, therapeutic strategies, and chemical markers that can be used to detect cow milk intolerance in infants. This book provides the most current scientific information on milk proteins, from structure to biological properties. It will be of great benefit for those interested in milk production, milk chemistry, and human health.
Book Synopsis Scientific Literature Reviews on Generally Recognized as Safe (GRAS) Food Ingredients by : Tracor Jitco, Inc
Download or read book Scientific Literature Reviews on Generally Recognized as Safe (GRAS) Food Ingredients written by Tracor Jitco, Inc and published by . This book was released on 1974 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Milk Proteins V2 written by Hugh McKenzie and published by Elsevier. This book was released on 2012-12-02 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: Milk Proteins: Chemistry and Molecular Biology, Volume II provides an extensive and detailed discussion on individual milk proteins. This volume focuses on caseins, which constitute the major group of milk proteins, and provides an understanding of the formation and structure of casein micelles. The topics discussed include the formation and structure of casein micelles; isolation, properties, and zone electrophoresis of whole casein; casein and its attack by rennin (chymosin); biochemistry of prorennin (prochymosin) and rennin (chymosin); minor milk proteins and enzymes; milk protein research and milk technology; and milk proteins in prospect. This book is a good reference for students majoring in protein chemistry, as well as protein chemists and biochemists conducting research on milk proteins.
Book Synopsis Genetic Engineering of Milk Proteins in Transgenic Animals by : Heng-Cherl Yom
Download or read book Genetic Engineering of Milk Proteins in Transgenic Animals written by Heng-Cherl Yom and published by . This book was released on 1991 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Casein written by and published by . This book was released on 1974 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Variability in True Protein, Casein, Nonprotein Nitrogen, and Proteolysis in High and Low Somatic Cell Milks by : Robert Joseph Verdi
Download or read book Variability in True Protein, Casein, Nonprotein Nitrogen, and Proteolysis in High and Low Somatic Cell Milks written by Robert Joseph Verdi and published by . This book was released on 1985 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of Some Minor Caseins and Proteose Peptones of Bovine Milk by : Eamon Padraig Fearghal O'Flaherty
Download or read book Characterization of Some Minor Caseins and Proteose Peptones of Bovine Milk written by Eamon Padraig Fearghal O'Flaherty and published by . This book was released on 1997 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: The physical, chemical and functional characteristics of food proteins are of fundamental importance to the behaviour of these proteins during processing, storage and ultimately on consumption of the food product, while their biological properties impart nutritional, physiological, biochemical and immunological qualities to the food. Of all food proteins, milk proteins are probably the most thoroughly characterised, chemically, physically and genetically. With the advent of modern analytical techniques, polymorphism of milk proteins can now be studied at the nucleotide level. The discovery of new milk protein gene alleles has enabled animals to be genotyped at birth, allowing for the selection of protein variants associated with specific dairy properties, eg. k-casein in bovine breeds. Expression analysis of native or modified genes with the use of in vitro transcription systems, mammary cell lines and transgenic animals has further improved knowledge of milk protein gene function. Alteration of the primary sequence of milk proteins by modern genetic engineering methods allows for changes in the three dimensional structure to be made, which offers the potential to modify protein functional characteristics. Although the proteins of bovine milk have been widely studies, some minor components of the casein and whey protein fractions have not been characterized to date. Although the significance of such minor components may be negligible, characterisation of such proteins may contribute to a better understanding of the complex milk protein system.
