Characterization Of Improved Sweet Sorghum Cultivars

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Characterization of Improved Sweet Sorghum Cultivars

Author : P. Srinivasa Rao
Publisher : Springer Science & Business Media
ISBN 13 : 8132207831
Total Pages : 134 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Characterization of Improved Sweet Sorghum Cultivars by : P. Srinivasa Rao

Download or read book Characterization of Improved Sweet Sorghum Cultivars written by P. Srinivasa Rao and published by Springer Science & Business Media. This book was released on 2012-10-05 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: A number of driving forces, including the soaring global crude oil prices and environmental concerns in both developed and developing nations has triggered a renewed interest in the recent years on the R&D of biofuel crops. In this regard, many countries across the globe are investing heavily in the bioenergy sector for R&D to increase their energy security and reduce their dependence on imported fossil fuels. Currently, most of the biofuel requirement is met by sugarcane in Brazil and corn in the United States, while biodiesel from rapeseed oil in Europe. Sweet sorghum has been identified as a unique biofuel feedstock in India since it is well adapted to Indian agro-climatic conditions and more importantly it does not jeopardize food security at the cost of fuel. Sweet sorghum [Sorghum bicolor (L.) Moench] is considered as a SMART new generation energy crop as it can accumulate sugars in its stalks similar to sugarcane, but without food¬¬-fuel trade-offs and can be cultivated in almost all temperate and tropical climatic conditions and has many other advantages. The grain can be harvested from the panicles at maturity. There is no single publication detailing the agronomic and biochemical traits of tropical sweet sorghum cultivars and hybrid parents. Hence, an attempt is made in this publication- “Characterization of improved sweet sorghum cultivars” to detail the complete description of cultivars. This book serves as a ready reference on the detailed characterization of different improved sweet sorghum genotypes following the PPVFRA guidelines for the researchers, entrepreneurs, farmers and other stakeholders to identify the available sweet sorghum cultivars and understand their yield potential in tropics.

Characterization of Improved Sweet Sorghum Cultivars

Author : P. Srinivasa Rao
Publisher : Springer Science & Business Media
ISBN 13 : 8132207823
Total Pages : 134 pages
Book Rating : 4.1/5 (322 download)

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Book Synopsis Characterization of Improved Sweet Sorghum Cultivars by : P. Srinivasa Rao

Advances in Sorghum Science

Author : Ratikanta Maiti
Publisher : CRC Press
ISBN 13 : 1000286908
Total Pages : 268 pages
Book Rating : 4.0/5 (2 download)

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Book Synopsis Advances in Sorghum Science by : Ratikanta Maiti

Download or read book Advances in Sorghum Science written by Ratikanta Maiti and published by CRC Press. This book was released on 2021-07-16 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, Advances in Sorghum Science: Botany, Production, and Crop Improvement, provides an easy-to-read and comprehensive treatment of the sorghum crop. With the world’s production of sorghum topping over 55 million tons annually, sorghum is very important for as a staple dietary food for much of the world as a rich source of micronutrients and macronutrients, as an ingredient in the processing of many foods, and as a source of fodder. The authors of the volume provide detailed information on sorghum from several disciplines and bring together recent literature under one umbrella. The book covers the various aspects of the sorghum crop, starting from its origin, to its domestication, and going on to biotechnology of the crop. It describes sorghum production, ideotypes, botany, physiology, abiotic and biotic factors affecting crop productivity, methods of cultivation, postharvest management, grain quality analysis for food processing, improvement of sorghum crop, and research advancements in breeding and biotechnology. This valuable resource will be helpful to researchers and scientists working to understand the relation between various disciplines and the implementation of new methods and technology for crop improvement and higher productivity. The multi-pronged approach will help to enable the increase sorghum productivity to meet the world’s growing demands.

Breeding Sorghum for Diverse End Uses

Author : Aruna C
Publisher : Woodhead Publishing
ISBN 13 : 0081018800
Total Pages : 450 pages
Book Rating : 4.0/5 (81 download)

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Book Synopsis Breeding Sorghum for Diverse End Uses by : Aruna C

Download or read book Breeding Sorghum for Diverse End Uses written by Aruna C and published by Woodhead Publishing. This book was released on 2018-08-22 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Breeding Sorghum for Diverse End Uses is a comprehensive overview of all significant global efforts for the genetic improvement of sorghum, a major crop of many semi-arid nations that is suitable for a huge range of uses, from human food, to biofuels. Split into two main sections, the book initially reviews the genetic suitability of sorghum for breeding, also providing the history of the genetic improvement of the grain. Finally, other sections look at specific breeding programs that could be improved in a number of areas, including human food, animal feed and industrial usage. Readers in academics, research, plant genetics and sorghum development will find this resource of great value. In addition, it is essential reading for engineers who utilize sorghum for food, feed and industrial materials in industry. Provides information on key advances in the genetic makeup of sorghum Allows plant breeders to apply this research to effectively breed new strains of sorghum that are dependent on final usage goals Includes the latest findings in each section to orient researchers to plans for future genetic enhancement

Lost Crops of Africa

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309176891
Total Pages : 405 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Lost Crops of Africa by : National Research Council

Download or read book Lost Crops of Africa written by National Research Council and published by National Academies Press. This book was released on 1996-02-14 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scenes of starvation have drawn the world's attention to Africa's agricultural and environmental crisis. Some observers question whether this continent can ever hope to feed its growing population. Yet there is an overlooked food resource in sub-Saharan Africa that has vast potential: native food plants. When experts were asked to nominate African food plants for inclusion in a new book, a list of 30 species grew quickly to hundreds. All in all, Africa has more than 2,000 native grains and fruitsâ€""lost" species due for rediscovery and exploitation. This volume focuses on native cereals, including: African rice, reserved until recently as a luxury food for religious rituals. Finger millet, neglected internationally although it is a staple for millions. Fonio (acha), probably the oldest African cereal and sometimes called "hungry rice." Pearl millet, a widely used grain that still holds great untapped potential. Sorghum, with prospects for making the twenty-first century the "century of sorghum." Tef, in many ways ideal but only now enjoying budding commercial production. Other cultivated and wild grains. This readable and engaging book dispels myths, often based on Western bias, about the nutritional value, flavor, and yield of these African grains. Designed as a tool for economic development, the volume is organized with increasing levels of detail to meet the needs of both lay and professional readers. The authors present the available information on where and how each grain is grown, harvested, and processed, and they list its benefits and limitations as a food source. The authors describe "next steps" for increasing the use of each grain, outline research needs, and address issues in building commercial production. Sidebars cover such interesting points as the potential use of gene mapping and other "high-tech" agricultural techniques on these grains. This fact-filled volume will be of great interest to agricultural experts, entrepreneurs, researchers, and individuals concerned about restoring food production, environmental health, and economic opportunity in sub-Saharan Africa. Selection, Newbridge Garden Book Club

Characterization of Sorghum Cultivars by Morphological, Chemical and Electrophoretic Tests

Author : S. M. Inamdar
Publisher :
ISBN 13 :
Total Pages : 82 pages
Book Rating : 4.:/5 (782 download)

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Book Synopsis Characterization of Sorghum Cultivars by Morphological, Chemical and Electrophoretic Tests by : S. M. Inamdar

Download or read book Characterization of Sorghum Cultivars by Morphological, Chemical and Electrophoretic Tests written by S. M. Inamdar and published by . This book was released on 2001 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Heterosis and Composition of Sweet Sorghum

Author : Rebecca J. Corn
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (76 download)

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Book Synopsis Heterosis and Composition of Sweet Sorghum by : Rebecca J. Corn

Download or read book Heterosis and Composition of Sweet Sorghum written by Rebecca J. Corn and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Sweet sorghum (Sorghum bicolor) has potential as a bioenergy feedstock due to its high yield potential and the production of simple sugars for fermentation. Sweet sorghum cultivars are typically tall, high biomass types with juicy stalks and high sugar concentration. These sorghums can be harvested, milled, and fermented to ethanol using technology similar to that used to process sugarcane. Sweet sorghum has advantages in that it can be planted by seed with traditional planters, is an annual plant that quickly produces a crop and fits well in crop rotations, and it is a very water-use efficient crop. Processing sweet sorghum is capital intensive, but it could fit into areas where sugarcane is already produced. Sweet sorghum could be timed to harvest and supply the sugar mill during the off season when sugarcane is not being processed, be fit into crop rotations, or used in water limiting environments. In these ways, sweet sorghum could be used to produce ethanol in the Southern U.S and other tropical and subtropical environments. Traditionally, sweet sorghum has been grown as a pureline cultivar. However, these cultivars produce low quantities of seed and are often too tall for efficient mechanical harvest. Sweet sorghum hybrids that use grain-type seed parents with high sugar concentrations are one way to overcome limitation to seed supply and to capture the benefits of heterosis. There are four objectives of this research. First to evaluate the importance of genotype, environment, and genotype-by-environment interaction effects on the sweet sorghum yield and composition. The second objective is to determine the presence and magnitude of heterosis effects for traits related to sugar production in sweet sorghum. Next: to study the ability of sweet sorghum hybrids and cultivars to produce a ratoon crop and determine the contribution of ratoon crops to total sugar yield. The final objective is to evaluate variation in composition of sweet sorghum juice and biomass. Sweet sorghum hybrids, grain-type sweet seed parents, and traditional cultivars that served as male parents were evaluated in multi-environment trials in Weslaco, College Station, and Halfway, Texas in 2007 and 2008. Both genotype and environment influenced performance, but environment had a greater effect than genotype on the composition of sweet sorghum juice and biomass yield. In comparing performance, elite hybrids produced fresh biomass and sugar yields similar to the traditional cultivars while overcoming the seed production limitations. High parent heterosis was expressed among the experimental hybrids for biomass yield, sugar yield and sugar concentration. Additional selection for combining ability would further enhance yields and heterosis in the same hybrid. Little variation was observed among hybrids for juice and biomass composition suggesting that breeding efforts should focus on yield before altering plant composition.

Optimization of In-Vitro Tissue Culture and Transformation System for Sweet Sorghum Cultivars Using Immature Inflorescence

Author : Logayn Abushal
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (138 download)

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Book Synopsis Optimization of In-Vitro Tissue Culture and Transformation System for Sweet Sorghum Cultivars Using Immature Inflorescence by : Logayn Abushal

Download or read book Optimization of In-Vitro Tissue Culture and Transformation System for Sweet Sorghum Cultivars Using Immature Inflorescence written by Logayn Abushal and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Background: Sweet sorghum is a multi-purpose crop that can be used for human food, animal feed, and bio-fuel production. This crop is drought-tolerant and can therefore be grown in arid and semi-arid regions especially in marginal areas in Egypt. Nevertheless, production of Sweet sorghum cannot coup with the increasing demand in consumption as a food, and fuel due to a lag in scientific research regarding its genetic improvement. Therefore, genetic improvement of Sweet sorghum is innately required so that new traits of economic importance could be introduced; however, this can only be achieved through the establishment of a robust in-vitro tissue culture system. Thus, this study aimed to establish an effective regeneration system for three different Sweet sorghum cultivars namely; Rex, Sugar Drip, and Ramada, using immature inflorescence explants. Subsequently, the cultivar Sugar was selected to examine its transformation efficiency using the microprojectile bombardment technology. Methods: For callus induction media, explants from immature inflorescences of different lengths (1.5- 16 cm), were grown over a combination of 2,4-D (0, 2, 4 & 6mg/L) with kinetin (0, 0.2 & 0.5mg/L). For regeneration media, BAP was the main cytokinin implemented in the three different treatments along with either: NAA, IAA, or IAA & TDZ, whereas TDZ was employed alone in a fourth treatment. The transformation system of the cultivar Sugar Drip was also developed using immature inflorescence explants. Co-bombardment was performed using the neomycin phosphotransferase II (emnptII) gene under the control of maize ubiquitin (emUbi1) promoter. The selection of putative transgenic plants was performed using paromomycin antibiotic. Results: The best embryogenic callus induction frequency was observed in Rex (77%) on modified MS media supplemented with 4mg/L 2,4-D + 0.2mg/L kinetin after six weeks from culture. However, Ramada and Sugar Drip had their highest callus induction rates of 93 and 94%, respectively, when 6mg/L 2,4-D + 0.2mg/L kinetin was used. The addition of 1mg/L IAA + 0.5mg/L BAP + 0.1mg/L TDZ had its best outcome in terms on shoot induction, shoot/callus number, and root formation. In Sugar Drip transformation, paromomycin eliminated most the non-transgenic plants from the putative transgenic ones. Eventually, 6 out of 348 bombarded samples were found to be transgenic after PCR screening. The percentage of transformation from two independent experiments was around 1.724%. Conclusion: PCR analysis of putative transformants revealed a transformation efficiency of 1.724%. Therefore, genetic transformation using particle bombardment has shown to be a successful method for the transformation of immature Sweet sorghum inflorescences with relatively high success rates.

Sorghum

Author : Ignacio A. Ciampitti
Publisher : John Wiley & Sons
ISBN 13 : 0891186271
Total Pages : 528 pages
Book Rating : 4.8/5 (911 download)

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Book Synopsis Sorghum by : Ignacio A. Ciampitti

Download or read book Sorghum written by Ignacio A. Ciampitti and published by John Wiley & Sons. This book was released on 2020-01-22 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sorghum is among the top five cereals and one of the key crops in global food security efforts. Sorghum is a resilient crop under high-stress environments, ensuring productivity and access to food when other crops fail. Scientists see the potential of sorghum as a main staple food in a future challenged by climate change. The contributors provide a comprehensive review of sorghum knowledge. The discussion covers genetic improvements, development of new hybrids, biotechnology, and physiological modifications. Production topics include water and nutrient management, rotations, and pest control. Final end uses, sorghum as a bioenergy crop, markets, and the future of sorghum are presented. IN PRESS! This book is being published according to the “Just Published” model, with more chapters to be published online as they are completed.

The Biomass Assessment Handbook

Author : Frank Rosillo-Calle
Publisher : Taylor & Francis
ISBN 13 : 1136554890
Total Pages : 293 pages
Book Rating : 4.1/5 (365 download)

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Book Synopsis The Biomass Assessment Handbook by : Frank Rosillo-Calle

Download or read book The Biomass Assessment Handbook written by Frank Rosillo-Calle and published by Taylor & Francis. This book was released on 2012-04-27 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increasing importance of biomass as a renewable energy source has lead to an acute need for reliable and detailed information on its assessment, consumption and supply. Responding to this need, and overcoming the lack of standardized measurement and accounting procedures, this handbook provides the reader with the skills to understand the biomass resource base, the tools to assess the resource, and explores the pros and cons of exploitation. Topics covered include assessment methods for woody and herbaceous biomass, biomass supply and consumption, remote sensing techniques as well as vital policy issues. International case studies, ranging from techniques for measuring tree volume to transporting biomass, help to illustrate step-by-step methods and are based on field work experience. Technical appendices offer a glossary of terms, energy units and other valuable resource data.

New Insights into Salinity Sensing, Signaling and Adaptation in Plants

Author : Giovanni Stefano
Publisher : Frontiers Media SA
ISBN 13 : 2889666948
Total Pages : 375 pages
Book Rating : 4.8/5 (896 download)

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Book Synopsis New Insights into Salinity Sensing, Signaling and Adaptation in Plants by : Giovanni Stefano

Download or read book New Insights into Salinity Sensing, Signaling and Adaptation in Plants written by Giovanni Stefano and published by Frontiers Media SA. This book was released on 2021-04-12 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt:

GGE Biplot Analysis

Author : Weikai Yan
Publisher : CRC Press
ISBN 13 : 1420040375
Total Pages : 287 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis GGE Biplot Analysis by : Weikai Yan

Download or read book GGE Biplot Analysis written by Weikai Yan and published by CRC Press. This book was released on 2002-08-28 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research data is expensive and precious, yet it is seldom fully utilized due to our ability of comprehension. Graphical display is desirable, if not absolutely necessary, for fully understanding large data sets with complex interconnectedness and interactions. The newly developed GGE biplot methodology is a superior approach to the graphical analys

A Study in Sorghum Bicolor

Author : Matthew Scott Bartek
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (873 download)

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Book Synopsis A Study in Sorghum Bicolor by : Matthew Scott Bartek

Download or read book A Study in Sorghum Bicolor written by Matthew Scott Bartek and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently designated as a bioenergy crop, Sorghum is rather unique as it can produce large quantities of cellulose or sugar which can be used to produce advanced biofuels or compounds. Sweet sorghum contains high levels of sugars and biomass sorghums consist primarily of ligno-cellulosic biomass. Improvement of both sorghum types is essential for maximizing production and conversion efficiency. Photoperiod sensitive sorghum is thought to maximize biomass production yet maturity influence on biomass production and composition is not fully understood. Utilizing sorghum for sugar production has increased efforts to develop sweet sorghums with sugar yields similar to sugarcane. Hybridization of these species has been investigated with, until recently, little success. Testing newly developed intergeneric hybrids and improvement of parents used in their creation will determine their feasibility and improve hybrid performance. Objectives of this research are multifaceted. First, analyze photoperiod sensitive sorghum in varying day length environments to determine maturity effects on plant phenotype, composition, and QTL detection. Second, analyze intergeneric sorghum x sugarcane hybrids to determine agronomic performance in relation to sugarcane. Lastly, introgress the iap allele into sweet sorghum females for use in intergeneric hybrid creation. Photoperiod sensitive sorghum RILs were evaluated in College Station and Weslaco, Texas and Puerto Rico which caused differential expression of plant maturity. Genetic control of trait expression was high for each location. Results indicate gradual induction of plant maturity increases detection of phenotypic QTL and detection of compositional QTL increases when maturity effects on plant phenotype are reduced. Intergeneric sorghum x sugarcane F1 hybrids were compared to sugarcane in Weslaco, Texas in 2011. Each hybrid expressed agronomic traits similar or better than that of the sugarcane variety. High levels of repeatability and genetic influence on trait expression were observed. Overall performance of the sugarcane variety was better than any individual hybrid tested. Introgression of iap into sweet sorghum was successful and generated seventeen new sweet sorghum female genotypes possessing the allele. Only two genotypes exhibited higher brix readings and both were later maturing than Tx3361. Height and maturity of all developed genotypes varied and desirability of developed lines was similar to Tx3361. The electronic version of this dissertation is accessible from http://hdl.handle.net/1969.1/151262

Bioremediation and Bioeconomy

Author : Majeti Narasimha Vara Prasad
Publisher : Elsevier
ISBN 13 : 0128028726
Total Pages : 730 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Bioremediation and Bioeconomy by : Majeti Narasimha Vara Prasad

Download or read book Bioremediation and Bioeconomy written by Majeti Narasimha Vara Prasad and published by Elsevier. This book was released on 2015-10-03 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioremediation and Bioeconomy provides a common platform for scientists from various backgrounds to find sustainable solutions to environmental issues, including the ever-growing lack of water resources which are under immense pressure due to land degradation, pollution, population explosion, urbanization, and global economic development. In addition, large amounts of toxic waste have been dispersed in thousands of contaminated sites and bioremediation is emerging as an invaluable tool for environmental clean-up. The book addresses these challenge by presenting innovative and cost-effective solutions to decontaminate polluted environments, including usage of contaminated land and waste water for bioproducts such as natural fibers, biocomposites, and fuels to boost the economy. Users will find a guide that helps scientists from various backgrounds find sustainable solutions to these environmental issues as they address the topical issues crucial for understanding new and innovative approaches for sustainable development. Provides a compilation of new information on phytoremediation not found in other books in the present market The first book to link phytoremediation and the bioeconomy Includes strategies to utilize contaminated soils for producing bioresources and co-generation of value chain and value additions products

Evaluation of Sweet Sorghum Cultivars as a Potential Rotation Crop in Molecular Farming and as a Feedstock Crop for Ethanol Production and Co-generation

Download Evaluation of Sweet Sorghum Cultivars as a Potential Rotation Crop in Molecular Farming and as a Feedstock Crop for Ethanol Production and Co-generation PDF Online Free

Author : Brett R. Weeden
Publisher :
ISBN 13 :
Total Pages : 30 pages
Book Rating : 4.:/5 (114 download)

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Book Synopsis Evaluation of Sweet Sorghum Cultivars as a Potential Rotation Crop in Molecular Farming and as a Feedstock Crop for Ethanol Production and Co-generation by : Brett R. Weeden

Download or read book Evaluation of Sweet Sorghum Cultivars as a Potential Rotation Crop in Molecular Farming and as a Feedstock Crop for Ethanol Production and Co-generation written by Brett R. Weeden and published by . This book was released on 2003 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt:

A-Z of Biorefinery

Author : Nuttha Thongchul
Publisher : Elsevier
ISBN 13 : 0128192496
Total Pages : 786 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis A-Z of Biorefinery by : Nuttha Thongchul

Download or read book A-Z of Biorefinery written by Nuttha Thongchul and published by Elsevier. This book was released on 2021-11-19 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: A-Z of Biorefinery: A Comprehensive View provides a comprehensive book that highlights and illustrates important topics relating to biorefineries, including associated theory, current and future research trends, available techniques and future challenges. This book will benefit a wide range of audiences, including students, engineers, scientists, practitioners, and those who are keen to explore more on biorefinery. Sections cover the availability of current technologies, constraints, market trends, recent system developments, and the concepts that enable modern biorefineries to utilize all kinds of biomass. This book is an essential resource for students, scientists, engineers and practitioners working in industry and academia. Covers the most important topics relating to biorefineries Provides related definitions, theories, overviews of methods, applications and important references Offers perspectives and concise reviews for each section Includes complete design case studies with tutorials

Sweet Sorghum

Author : Lila Rogers
Publisher :
ISBN 13 : 9781536153866
Total Pages : 118 pages
Book Rating : 4.1/5 (538 download)

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Book Synopsis Sweet Sorghum by : Lila Rogers

Download or read book Sweet Sorghum written by Lila Rogers and published by . This book was released on 2019-05-11 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sweet sorghum is a cereal that belongs to the species Sorghum bicolor (L) Moench. Although the crop is reportedly native to Africa, it is grown worldwide largely because it thrives well under wide rainfall, varied day lengths, and varied soil conditions and can tolerate varying degrees of biotic and abiotic factors and stresses. In Sweet Sorghum: Characteristics, Cultivation and Uses, the authors review the physiology and adaptation of sweet sorghum crops to varied environmental and climatic conditions across Sub-Saharan Africa. The authors also discuss the saline soil distribution and cultivation of sweet sorghum in China. Soil salinization is one of the most prominent environmental problems in the world, which limits crop yield and productivity seriously. Today, about 20% of the worlds cultivated land and nearly half of all irrigated lands are affected by salinity. Lastly, the utilization of sweet sorghum bagasse as raw material in the development of an environmentally friendly particleboard bonded with a natural adhesive without the addition of harmful chemical substances such as citric acid was carried out. The effectiveness of several manufacturing conditions such as pre-treatment of particles before hot pressing condition, citric acid contents, pressing temperature and time, and sucrose addition were investigated.