Cell Viability Assays

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Mammalian Cell Viability

Author : Martin J. Stoddart
Publisher : Humana Press
ISBN 13 : 9781617791079
Total Pages : 0 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis Mammalian Cell Viability by : Martin J. Stoddart

Download or read book Mammalian Cell Viability written by Martin J. Stoddart and published by Humana Press. This book was released on 2011-04-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Angiogenesis Assays

Author : Carolyn A. Staton
Publisher : John Wiley & Sons
ISBN 13 : 047002934X
Total Pages : 410 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Angiogenesis Assays by : Carolyn A. Staton

Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.

Genotoxicity

Author : Marcelo Larramendy
Publisher : BoD – Books on Demand
ISBN 13 : 1789234182
Total Pages : 124 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Genotoxicity by : Marcelo Larramendy

Download or read book Genotoxicity written by Marcelo Larramendy and published by BoD – Books on Demand. This book was released on 2018-07-11 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to provide an overview of the different genotoxicants and their effects on living organisms, including humans. The contributions made by the specialists in this field of research are gratefully acknowledged. We hope that the information presented in this book will meet the expectations and needs of all those interested in the different aspects of the genotoxicity field. The publication of this book is of great importance to those scientists, pharmacologists, physicians and veterinarians, as well as engineers, teachers, graduate students and administrators of environmental programmes, who make use of these investigations to understand both the basic and applied genotoxic aspects of known and new xenobiotics, and to guide them in their future investigations.

Polyamine Protocols

Author : David M. L. Morgan
Publisher : Springer Science & Business Media
ISBN 13 : 1592595650
Total Pages : 173 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Polyamine Protocols by : David M. L. Morgan

Download or read book Polyamine Protocols written by David M. L. Morgan and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt: A unique collection of hands-on enzyme assay techniques to study polyamines and their function. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. The methods are presented by leading researchers who have perfected them to a high art, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Cancer Cell Culture

Author : Ian A. Cree
Publisher : Humana Press
ISBN 13 : 9781493956579
Total Pages : 502 pages
Book Rating : 4.9/5 (565 download)

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Book Synopsis Cancer Cell Culture by : Ian A. Cree

Download or read book Cancer Cell Culture written by Ian A. Cree and published by Humana Press. This book was released on 2016-08-23 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.

Cytotoxicity

Author : Erman Salih Istifli
Publisher : BoD – Books on Demand
ISBN 13 : 1789847540
Total Pages : 120 pages
Book Rating : 4.7/5 (898 download)

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Book Synopsis Cytotoxicity by : Erman Salih Istifli

Download or read book Cytotoxicity written by Erman Salih Istifli and published by BoD – Books on Demand. This book was released on 2019-10-02 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Compensating for cytotoxicity in the multicellular organism by a certain level of cellular proliferation is the primary aim of homeostasis. In addition, the loss of cellular proliferation control (tumorigenesis) is at least as important as cytotoxicity, however, it is a contrasting trauma. With the disruption of the delicate balance between cytotoxicity and proliferation, confrontation with cancer can inevitably occur. This book presents important information pertaining to the molecular control of the mechanisms of cytotoxicity and cellular proliferation as they relate to cancer. It is designed for students and researchers studying cytotoxicity and its control.

In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

Author : Dhanya Sunil
Publisher : CRC Press
ISBN 13 : 1351751522
Total Pages : 156 pages
Book Rating : 4.3/5 (517 download)

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Book Synopsis In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development by : Dhanya Sunil

Download or read book In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development written by Dhanya Sunil and published by CRC Press. This book was released on 2017-05-18 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials. In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates in a wide category of anticancer assays demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules. The major emphasis of the book is to present those bioassays which can be readily set up and practiced in any laboratory with limited funds, facilities or technical know-how.

Immunology: Overview and Laboratory Manual

Author : Tobili Y. Sam-Yellowe
Publisher : Springer Nature
ISBN 13 : 3030646866
Total Pages : 461 pages
Book Rating : 4.0/5 (36 download)

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Book Synopsis Immunology: Overview and Laboratory Manual by : Tobili Y. Sam-Yellowe

Download or read book Immunology: Overview and Laboratory Manual written by Tobili Y. Sam-Yellowe and published by Springer Nature. This book was released on 2021-08-01 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

Cell Viability Assays

Author : Oliver Friedrich
Publisher : Springer Nature
ISBN 13 : 1071630520
Total Pages : 501 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Cell Viability Assays by : Oliver Friedrich

Download or read book Cell Viability Assays written by Oliver Friedrich and published by Springer Nature. This book was released on 2023-05-04 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

Cell-Based Assays for High-Throughput Screening

Author : Paul A. Clemons
Publisher : Humana Press
ISBN 13 : 9781627039079
Total Pages : 0 pages
Book Rating : 4.0/5 (39 download)

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Book Synopsis Cell-Based Assays for High-Throughput Screening by : Paul A. Clemons

Download or read book Cell-Based Assays for High-Throughput Screening written by Paul A. Clemons and published by Humana Press. This book was released on 2014-11-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Cancer Cell Culture

Author : Simon P. Langdon
Publisher : Springer Science & Business Media
ISBN 13 : 1592594069
Total Pages : 346 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Cancer Cell Culture by : Simon P. Langdon

Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.

Cytotoxicity

Author : Tulay Askin Celik
Publisher : BoD – Books on Demand
ISBN 13 : 1789234301
Total Pages : 280 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Cytotoxicity by : Tulay Askin Celik

Download or read book Cytotoxicity written by Tulay Askin Celik and published by BoD – Books on Demand. This book was released on 2018-07-25 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book Cytotoxicity is aimed to be an essential reading to all medical students, biologists, biochemists and professionals involved in the field of toxicology. This book is a useful and ideal guide for novice researchers interested in learning research methods to study cytotoxic bioactive compounds. The parts of this book describe the replacement and different applications of the cytotoxic agents. All chapters are written by paramount experts in cytotoxicity research. This will hopefully stimulate more research initiatives, funding, and critical insight into the already increasing demand for cytotoxicity researches that have been evidenced worldwide.

Protocol Handbook for Cancer Biology

Author : Gauri Misra
Publisher : Academic Press
ISBN 13 : 0323898173
Total Pages : 222 pages
Book Rating : 4.3/5 (238 download)

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Book Synopsis Protocol Handbook for Cancer Biology by : Gauri Misra

Download or read book Protocol Handbook for Cancer Biology written by Gauri Misra and published by Academic Press. This book was released on 2021-02-12 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocol Handbook for Cancer Biology brings together a comprehensive collection of the methods used for cancer assessment, diagnostics, and therapeutics. Various protocols are discussed along with alternative strategies, including the advantages and limitations of techniques that have been used in labs globally. These protocols are presented by cancer biology experts based on their real-world experience. The protocols in this book will be a valuable resource for cancer researchers and graduate students, who can utilize the techniques described to conduct research more efficiently and successfully. Presents comprehensive protocols used for cancer assessment, diagnostics, and therapeutics all in one place Encompasses alternative strategies considering the requirements of the end user and taking into consideration diverse research settings Discusses limitations and advantages of each method in experimental design and execution, thus saving time during the research process

Cytotoxicity

Author : Sonia Soloneski
Publisher : BoD – Books on Demand
ISBN 13 : 1839687266
Total Pages : 123 pages
Book Rating : 4.8/5 (396 download)

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Book Synopsis Cytotoxicity by : Sonia Soloneski

Download or read book Cytotoxicity written by Sonia Soloneski and published by BoD – Books on Demand. This book was released on 2021-09-15 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book, Cytotoxicity - New Insights into Toxic Assessment, is intended to present some strategies, methods, interpretations and recent advances in order to facilitate scientific research on in vitro toxic responses, presenting both theoretical and practical aspects.

Biochemical Ecotoxicology

Author : Francois Gagne
Publisher : Elsevier
ISBN 13 : 012411623X
Total Pages : 282 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Biochemical Ecotoxicology by : Francois Gagne

Download or read book Biochemical Ecotoxicology written by Francois Gagne and published by Elsevier. This book was released on 2014-07-07 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical Ecotoxicology: Principles and Methods presents practical approaches to biochemical ecotoxicology experiments for environmental protection and conservation. With its methodical, stepped approach this essential reference introduces readers to current techniques for toxicity endpoint testing, suitable for laboratories of any size and budget. Each chapter presents a state-of-the-art principle, a quick and inexpensive procedure (including appropriate reagents), case studies, and demonstrations on how to analyze your results. Generic techniques are covered, suitable for a variety of organisms, as well as high-throughput techniques like quantitative polymerase chain reactions and enzyme-linked immunoassays. Cutting-edge approaches, including gPCR arrays and lipidomic techniques, are also included, making this is an essential reference for anyone who needs to assess environmental toxicity. Practical, cost-effective approaches to assess environmental toxicity endpoints for all types of organism Presents theory, methods, case studies and information on how to analyze results State-of-the-art techniques, such as ‘omics’ approaches to toxicology

Bioluminescence and Chemiluminescence

Author : A. Roda
Publisher : Wiley
ISBN 13 : 9780471987338
Total Pages : 648 pages
Book Rating : 4.9/5 (873 download)

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Book Synopsis Bioluminescence and Chemiluminescence by : A. Roda

Download or read book Bioluminescence and Chemiluminescence written by A. Roda and published by Wiley. This book was released on 1999-05-27 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioluminescence is the natural phenomenon found in light emitting organisms while chemiluminescence is a similar phenomenon in chemical reactions. This book contains the proceedings of one of the world's leading conferences in this field of study. As with previous, well-received publications of this conference, this book showcases the conference's growing importance.

Chaperones

Author : Stuart K. Calderwood
Publisher : Humana
ISBN 13 : 9781071633410
Total Pages : 0 pages
Book Rating : 4.6/5 (334 download)

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Book Synopsis Chaperones by : Stuart K. Calderwood

Download or read book Chaperones written by Stuart K. Calderwood and published by Humana. This book was released on 2023-08-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone function and biology; the emerging role of the extracellular HSPs; and the use of chaperones as biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Chaperones: Methods and Protocols, Second Edition is a valuable resource for all researchers who want to learn more about this interesting and developing field.