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Capillary Electrophoresis Of Proteins And Peptides
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Book Synopsis Capillary Electrophoresis of Proteins and Peptides by : Mark A. Strege
Download or read book Capillary Electrophoresis of Proteins and Peptides written by Mark A. Strege and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Book Synopsis Capillary Electrophoresis of Proteins and Peptides by : Nguyet Thuy Tran
Download or read book Capillary Electrophoresis of Proteins and Peptides written by Nguyet Thuy Tran and published by Humana. This book was released on 2016-07-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.
Book Synopsis Introduction to capillary electrophoresis of proteins and peptides by : H.E. Schwartz
Download or read book Introduction to capillary electrophoresis of proteins and peptides written by H.E. Schwartz and published by . This book was released on 1993 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis of Proteins by : Tim Wehr
Download or read book Capillary Electrophoresis of Proteins written by Tim Wehr and published by CRC Press. This book was released on 1998-10-20 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."
Book Synopsis Clinical Applications of Capillary Electrophoresis by : Stephen M. Palfrey
Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.
Book Synopsis Methods for High-sensitivity Separations of Proteins and Peptides Using Capillary Electrophoresis and Microcolumn Liquid Chromatography by : Kelly Ann Cobb
Download or read book Methods for High-sensitivity Separations of Proteins and Peptides Using Capillary Electrophoresis and Microcolumn Liquid Chromatography written by Kelly Ann Cobb and published by . This book was released on 1991 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Peptides and Proteins by Electrophoretic Techniques by : Angela R. Piergiovanni
Download or read book Analysis of Peptides and Proteins by Electrophoretic Techniques written by Angela R. Piergiovanni and published by MDPI. This book was released on 2019-07-23 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Book Synopsis Separation of Proteins and Peptides by Capillary Electrophoresis by : Herb Schwartz
Download or read book Separation of Proteins and Peptides by Capillary Electrophoresis written by Herb Schwartz and published by . This book was released on 1994 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis New Methods in Peptide Mapping for the Characterization of Proteins by : William S. Hancock
Download or read book New Methods in Peptide Mapping for the Characterization of Proteins written by William S. Hancock and published by CRC Press. This book was released on 1995-10-23 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Book Synopsis Capillary Electrophoresis in Biotechnology and Environmental Analysis by : Hasan Parvez
Download or read book Capillary Electrophoresis in Biotechnology and Environmental Analysis written by Hasan Parvez and published by CRC Press. This book was released on 2023-01-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.
Book Synopsis CE in Biotechnology: Practical Applications for Protein and Peptide Analyses by : Anthony B. Chen
Download or read book CE in Biotechnology: Practical Applications for Protein and Peptide Analyses written by Anthony B. Chen and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.
Book Synopsis Separation of Proteins and Peptides by Capillary Electrophoresis by : Herb E. Schwartz
Download or read book Separation of Proteins and Peptides by Capillary Electrophoresis written by Herb E. Schwartz and published by . This book was released on 1992 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Separation of Peptides and Proteins by Capillary Electrophoresis by : Kimberly Fay Greve
Download or read book Separation of Peptides and Proteins by Capillary Electrophoresis written by Kimberly Fay Greve and published by . This book was released on 1995 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Trace Analysis of Peptides and Proteins by Capillary Electrophoresis by :
Download or read book Trace Analysis of Peptides and Proteins by Capillary Electrophoresis written by and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Ultrasensitive Detection of Proteins and Peptides by Capillary Electrophoresis Affinity Assays by : Igor German
Download or read book Ultrasensitive Detection of Proteins and Peptides by Capillary Electrophoresis Affinity Assays written by Igor German and published by . This book was released on 2001 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis of Amino Acids, Peptides and Proteins by : Ziad El Rassi
Download or read book Capillary Electrophoresis of Amino Acids, Peptides and Proteins written by Ziad El Rassi and published by . This book was released on 1995 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Proteins and Peptides by On-column Preconcentration Capillary Liquid Chromatography and Capillary Electrophoresis by : Hong Shen
Download or read book Analysis of Proteins and Peptides by On-column Preconcentration Capillary Liquid Chromatography and Capillary Electrophoresis written by Hong Shen and published by . This book was released on 1998 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
