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Bioluminescence And Fluorescence For In Vivo Imaging
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Author :Lubov Brovko Publisher :SPIE-International Society for Optical Engineering ISBN 13 :9780819482471 Total Pages :148 pages Book Rating :4.4/5 (824 download)
Book Synopsis Bioluminescence and Fluorescence for in Vivo Imaging by : Lubov Brovko
Download or read book Bioluminescence and Fluorescence for in Vivo Imaging written by Lubov Brovko and published by SPIE-International Society for Optical Engineering. This book was released on 2010 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioluminescence methods are gaining increased attention due to their sensitivity, selectivity, and simplicity, along with the fact that bioluminescence can be monitored bothin vitroandin vivo. This book introduces bioluminescence and fluorescence systems, along with the principles of their application forin vivoimaging of intracellular processes, and covers recent developments in optical (bioluminescence and fluorescence) imaging in cell biology. This book is intended for scientists and students involved in basic cell physiology research, as well as industry professionals, engineers, and managers involved in drug discovery and pre-clinical drug development. It discusses the practical aspects of luminescencein vivoimaging for monitoring intracellular processes. While some basic knowledge of biochemistry and biophysics is preferable, the book includes a brief review of fundamental principles to allow those not familiar with these disciplines to grasp basic concepts.
Book Synopsis Trypanosomatids by : Paul A. M. Michels
Download or read book Trypanosomatids written by Paul A. M. Michels and published by Humana. This book was released on 2020-03-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases. Chapters 14, 15, 16, 23, 24, 30, and 48 are open access under a CC BY 4.0 license.
Book Synopsis Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by : Gérald Thouand
Download or read book Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 written by Gérald Thouand and published by Springer. This book was released on 2016-01-12 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English. /div
Book Synopsis Imaging Living Cells by : Rosario Rizzuto
Download or read book Imaging Living Cells written by Rosario Rizzuto and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intended for bench-top use, this lab manual is suitable for both scientists and graduate students, since it combines an update on the most advanced imaging procedures with detailed protocols. Examples, carefully selected from the wide repertoire of cell pyhsiology, cover such different functional aspects as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.
Book Synopsis Handbook of In Vivo Chemistry in Mice by : Katsunori Tanaka
Download or read book Handbook of In Vivo Chemistry in Mice written by Katsunori Tanaka and published by John Wiley & Sons. This book was released on 2020-04-27 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides timely, comprehensive coverage of in vivo chemical reactions within live animals This handbook summarizes the interdisciplinary expertise of both chemists and biologists performing in vivo chemical reactions within live animals. By comparing and contrasting currently available chemical and biological techniques, it serves not just as a collection of the pioneering work done in animal-based studies, but also as a technical guide to help readers decide which tools are suitable and best for their experimental needs. The Handbook of In Vivo Chemistry in Mice: From Lab to Living System introduces readers to general information about live animal experiments and detection methods commonly used for these animal models. It focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release. Topics include: currently available mouse models; biocompatible fluorophores; radionuclides for radiodiagnosis/radiotherapy; live animal imaging techniques such as positron emission tomography (PET) imaging; magnetic resonance imaging (MRI); ultrasound imaging; hybrid imaging; biocompatible chemical reactions; ligand-directed nucleophilic substitution chemistry; biorthogonal prodrug release strategies; and various selective targeting strategies for live animals. -Completely covers current techniques of in vivo chemistry performed in live animals -Describes general information about commonly used live animal experiments and detection methods -Focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release -Places emphasis on material properties required for the development of appropriate compounds to be used for imaging and therapeutic purposes in preclinical applications Handbook of In Vivo Chemistry in Mice: From Lab to Living System will be of great interest to pharmaceutical chemists, life scientists, and organic chemists. It will also appeal to those working in the pharmaceutical and biotechnology industries.
Book Synopsis In Vivo Imaging in Pharmacological Research by : Nicolau Beckmann
Download or read book In Vivo Imaging in Pharmacological Research written by Nicolau Beckmann and published by Frontiers Media SA. This book was released on 2017-08-08 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery and development of a biological active molecule with therapeutic properties is an ever increasing complex task, highly unpredictable at the early stages and marked, in the end, by high rates of failure. As a consequence, the overall process leading to the production of a successful drug is very costly. The improvement of the net outcome in drug discovery and development would require, amongst other important factors, a good understanding of the molecular events that characterize the disease or pathology in order to better identify likely targets of interest, to optimize the interaction of an active agent (small molecule or macromolecule of natural or synthetic origin) with those targets, and to facilitate the study of the pharmacokinetics, pharmacodynamics and toxicity of an active agent in suitable models and in human subjects. The objective of this Research Topic is to highlight new developments and applications of imaging techniques with the objective of performing pharmacological studies in vivo, in animal models and in humans. In the domain of drug discovery, the pharmacological and biomedical questions constitute the center of attention. In this sense, it is fundamental to keep in mind the strengths and limitations of each analytical or imaging technique. At the end, the judicious application of the technique with the aim of supporting the search for answers to manifold questions arising during a long and painstaking path provides a continuous role for imaging within the complex area of drug discovery and development.
Book Synopsis Bioluminescence and Chemiluminescence by : Marlene A. Deluca
Download or read book Bioluminescence and Chemiluminescence written by Marlene A. Deluca and published by Elsevier. This book was released on 2013-09-11 with total page 813 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications is a compendium of papers presented at the second International Symposium on Analytical Applications of Bioluminescene and Chemiluminescence in San Diego, California on August 26-28, 1980. Part I deals with chemilunescence and excited states including topics on the spectroscopy of the solvent cage — generation and characteristics of the excited state and the three features of chemi- and bioluminescence. Part II deals with events prior to producing luminol-dependent chemiluminescence; this part also examines the effects of stimulants on membrane potential. Part III discusses bacterial bioluminescence and analyzes the properties of a lumazine protein from a bioluminescent bacterium. This part also analyzes accessory enzymes responsible for such bacterial bioluminescence. Part IV examines the chemistry of firefly bioluminescence and presents the formula of three reactions catalyzed by firefly luciferase. Part V analyzes bioluminescence from other sources such as the earthworm and land snails. Part VI discusses the applications of bioluminescence in clinical chemistry, soil science, and marine biology. Part VII deals with the future uses of bioluminescence, while Part VIII is an abstract of other papers dealing with this subject. This collection can be helpful for biologists, zoologists, micro-biologists, marine biologists, and researchers dealing with bio-chemistry and related disciplines.
Book Synopsis Fluorescence Imaging and Biological Quantification by : Raquel Seruca
Download or read book Fluorescence Imaging and Biological Quantification written by Raquel Seruca and published by CRC Press. This book was released on 2017-09-06 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.
Book Synopsis Reporter Gene Imaging by : Purnima Dubey
Download or read book Reporter Gene Imaging written by Purnima Dubey and published by Humana Press. This book was released on 2018-06-02 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Bioluminescence:chemicalprinciplesandmethods(3rdedition) by : Shimomura Osamu
Download or read book Bioluminescence:chemicalprinciplesandmethods(3rdedition) written by Shimomura Osamu and published by World Scientific. This book was released on 2019-06-06 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the bible of bioluminescence and a must-read not only for the students but for those who work in various fields relating to bioluminescence. It summarizes current structural information on all known bioluminescent systems in nature, from well-studied ones to those that have been seldom investigated.This book remains an important source of chemical knowledge on bioluminescence and, since the second edition's publication in 2012, has been revised to include major developments in two systems: earthworm Fridericia and higher fungi whose luciferins have been elucidated and synthesized. These two new luciferins represent an essential addition to seven previously known, with fully rewritten sections covering this new subject matter.
Book Synopsis Imaging from Cells to Animals In Vivo by : Margarida Barroso
Download or read book Imaging from Cells to Animals In Vivo written by Margarida Barroso and published by CRC Press. This book was released on 2020-12-03 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.
Book Synopsis Imaging from Cells to Animals In Vivo by : Margarida Barroso
Download or read book Imaging from Cells to Animals In Vivo written by Margarida Barroso and published by CRC Press. This book was released on 2020-12-03 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging. Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system. Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography and super-resolution imaging. Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior. Addresses the impact on discovery, such as cellular function as implicated in human disease and translational medicine, for example in cancer diagnosis.
Download or read book Bioluminescence written by Sung-Bae Kim and published by Springer Nature. This book was released on 2022-07-12 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.
Download or read book Bioluminescence written by David Lapota and published by BoD – Books on Demand. This book was released on 2012-02-01 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: We now find ourselves utilizing luciferase - luciferin proteins, ATP, genes and the whole complex of these interactions to observe and follow the progress or inhibition of tumors in animal models by measuring bioluminescence intensity, spatially and temporally using highly sophisticated camera systems. This book describes applications in preclinical oncology research by bioluminescence imaging (BLI) with a variety of applications. Chapters describe current methodologies for rapid detection of contaminants using the Milliflex system, and the use of bioluminescence resonance energy transfer (BRET) technology for monitoring physical interactions between proteins in living cells. Others are using bioluminescent proteins for high sensitive optical reporters imaging in living animals, developing pH-tolerant luciferase for brighter in vivo imaging, and oscillation characteristics in bacterial bioluminescence. The book also contains descriptions of the long-term seasonal characteristics of oceanic bioluminescence and the responsible planktonic species producing bioluminescence. Such studies are few and rare.
Book Synopsis Methods in Cellular Imaging by : Ammasi Periasamy
Download or read book Methods in Cellular Imaging written by Ammasi Periasamy and published by Springer. This book was released on 2013-05-27 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.
Book Synopsis Bioluminescence and Chemiluminescence by :
Download or read book Bioluminescence and Chemiluminescence written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Handbook of Neuro-Oncology Neuroimaging by : Herbert B. Newton
Download or read book Handbook of Neuro-Oncology Neuroimaging written by Herbert B. Newton and published by Elsevier. This book was released on 2022-08-25 with total page 1020 pages. Available in PDF, EPUB and Kindle. Book excerpt: With treatment approaches and the field of neuro-oncology neuroimaging changing rapidly, this third edition of the Handbook of Neuro-Oncology Neuroimaging is very relevant to those in the field, providing a single-source, comprehensive, reference handbook of the most up-to-date clinical and technical information regarding the application of neuroimaging techniques to brain tumor and neuro-oncology patients. This new volume will have updates on all of the material from the second edition, and in addition features several new important chapters covering diverse topics such as imaging for the use of Laser Interstitial Thermal Therapy, advanced imaging techniques in radiation therapy, therapeutic treatment fields, response assessment in clinical trials, surgical planning of neoplastic disease of the spine, and more. Sections first overview neuro-oncological disorders before delving into the physics and basic science of neuroimaging and great focus on CT and MRI. The book then focuses on advances in the neuroimaging of brain tumors and neuroimaging of specific tumor types. There is also discussion of neuroimaging of other neuro-oncological syndromes. This book will serve as a resource of background information to neuroimaging researchers and basic scientists with an interest in brain tumors and neuro-oncology. Summarizes translational research on brain imaging for brain tumors Discusses limitations of neuroimaging for diagnosis and treatment Presents advanced imaging technologies, including CT, MRI, and PET Contains new coverage on Laser Interstitial Thermal Therapy, radiation therapy, clinical trials, and more
