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Author : Yuichiro Takagi
Publisher :
ISBN 13 :
Total Pages : 230 pages
Book Rating : 4.:/5 (41 download)
Download or read book Biochemical Characterization of RNA Polymerase II written by Yuichiro Takagi and published by . This book was released on 1997 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : John Crickard
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (959 download)
Download or read book A Biochemical Characterization of Transcription Elongation Factors that Facilitate the Processivity of RNA Polymerase II. written by John Crickard and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The processivity of RNA Polymerase II across the body of a gene is a complex anddynamic process that involves the contribution of many transcription elongation factors.These elongation factors play a variety of roles including maintenance of RNAPII activity,maintenance of chromatin, and regulation of chromatin structure. The roles of elongationfactors highlight two strategies utilized by the cell to promote transit of RNAPII acrossthe body of a gene. The overall goal of this work was to gain mechanistic insight, basedon in vitro models, into RNAPII processivity, and how elongation factors contribute to thisprocess. In this dissertation, I demonstrate that a region of the H2B tail domain, knownas the H2B repression domain (HBR), helps Spt16-Pob3 (FACT) remove H2A/B dimersfrom the nucleosome. Then I show that Spt4/5, a universally conserved transcriptionelongation factor, maintains active RNAPII through interactions with the non-templatestrand of DNA. I also find that eukaryotic specific regions of Spt5 may recognize certainconformations of RNAPII. Finally, I identify a novel strategy utilized by Spt4/5 to act onspecific intermediate states to relocate RNAPII with in the nucleosome. This observationsuggests that nucleosomes may not simply act as a roadblock to transcription, but mayserve a regulatory function during transcription elongation. Together these findingsadvance our understanding of how elongation factors contribute to the processivity ofRNAPII, and how that effects gene regulation.
Author : Shaharum Shamsuddin
Publisher :
ISBN 13 :
Total Pages : 592 pages
Book Rating : 4.:/5 (54 download)
Download or read book Biochemical Characterization of the Interactions Between a Transcription Factor, CTCF, and Its Partners Y-Box Binding Protein-1 and the Large Subunit of RNA Polymerase II written by Shaharum Shamsuddin and published by . This book was released on 2002 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Eugene Thaddeus Butler
Publisher :
ISBN 13 :
Total Pages : 464 pages
Book Rating : 4.:/5 (27 download)
Download or read book The Detection, Purification, and Biochemical Characterization of an RNA Polymerase Induced by Bacteriophage SP6 written by Eugene Thaddeus Butler and published by . This book was released on 1978 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sankar Adhya
Publisher : Elsevier
ISBN 13 : 0080522599
Total Pages : 833 pages
Book Rating : 4.0/5 (85 download)
Download or read book RNA Polymerase and Associated Factors, Part C written by Sankar Adhya and published by Elsevier. This book was released on 2003-12-18 with total page 833 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
Author : Shaharum Shamsuddin
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (54 download)
Download or read book Biochemical Characterization of the Interactions Between a Transcription Factor, CTCF, and Its Partners Y-Box Binding Protein-1 and the Large Subunit of RNA Polymerase II written by Shaharum Shamsuddin and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Henri C. Buc
Publisher : Royal Society of Chemistry
ISBN 13 : 0854041346
Total Pages : 353 pages
Book Rating : 4.8/5 (54 download)
Download or read book RNA Polymerases as Molecular Motors written by Henri C. Buc and published by Royal Society of Chemistry. This book was released on 2009-04-16 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, written by expert scientists in the field, analyses how these diverse fields of research interact on a specific example - RNA polymerase. The book concentrates on RNA polymerases because they play a central role among all the other machines operating in the cell and are the target of a wide range of regulatory mechanisms. They have also been the subject of spectacular advances in their structural understanding in recent years, as testified by the attribution of the Nobel prize in chemistry in 2006 to Roger Kornberg. The book focuses on two aspects of the transcription cycle that have been more intensively studied thanks to this increased scientific cooperation - the recognition of the promoter by the enzyme, and the achievement of consecutive translocation steps during elongation of the RNA product. Each of these two topics is introduced by an overview, and is then presented by worldwide experts in the field, taking the viewpoint of their specialty. The overview chapters focus on the mechanism-structure interface and the structure-machine interface while the individual chapters within each section concentrate more specifically on particular processes-kinetic analysis, single-molecule spectroscopy, and termination of transcription, amongst others. Specific attention has been paid to the newcomers in the field, with careful descriptions of new emerging techniques and the constitution of an atlas of three-dimensional pictures of the enzymes involved.
Author : Sankar Adhya
Publisher : Elsevier
ISBN 13 : 0080522602
Total Pages : 715 pages
Book Rating : 4.0/5 (85 download)
Download or read book RNA Polymerase and Associated Factors, Part D written by Sankar Adhya and published by Elsevier. This book was released on 2003-12-18 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Models and methods for studying polymerase translocation * Assay for movements of RNA polymerase along DNA * Engineering of elongation complexes of bacterial and yeast RNA polymerases
Author : Claudia M. D'Abramo
Publisher :
ISBN 13 :
Total Pages : 404 pages
Book Rating : 4.:/5 (253 download)
Download or read book Biochemical Characterization of the BVDV RNA-dependent RNA Polymerase During Initiation and Elongation of RNA Synthesis written by Claudia M. D'Abramo and published by . This book was released on 2006 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The RNA-dependent RNA polymerase (RdRp) of viruses belonging to the Flaviviridae family, including the hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV) is critical for viral replication. The major goal of this PhD study was to biochemically characterize the role of the polymerase during initiation and elongation of RNA synthesis, utilizing the BVDV RdRp as a model system. We showed that the BVDV polymerase efficiently incorporates chain-terminating nucleoside analogues, which ultimately arrest RNA synthesis. The incorporated chain-terminators, however, can be removed from the primer terminus in the presence of pyrophosphate (PPi). These results suggest that the phosphorolytic excision of incorporated chain-terminators is a possible mechanism that can diminish the efficiency of this class of compounds against viral RdRps. The chain-terminators then served as valuable tools in subsequent experiments to analyze the functional role(s) of the RdRp-associated GTP-specific binding site (G-site) and the consequences of GTP binding during the initiation of RNA synthesis. The results provide biochemical evidence for the existence of a G-site in the BVDV enzyme, and suggest that GTP binding controls template positioning during de novo initiation. Finally, through the development of a novel ribonuclease-based footprinting assay, it was determined that catalytically active complexes contact the newly synthesized RNA during elongation of RNA synthesis with approximately 6-7 base pairs. The polymerase moves along the template according to the position where RNA synthesis is arrested. Taken together, this study provides novel insight into mechanisms involved during initiation and elongation of RNA replication of viruses belonging to the Flaviviridae family. The ability of RdRps to excise incorporated chain-terminators points to possible shortcomings of nucleoside analogue inhibitors that are under development as antiviral agents for the treatment of infection with HCV." --
Author : Samson T. Jacob
Publisher : CRC Press
ISBN 13 : 1351080318
Total Pages : 203 pages
Book Rating : 4.3/5 (51 download)
Download or read book Enzymes of Nucleic Acid Synthesis and Modification written by Samson T. Jacob and published by CRC Press. This book was released on 2018-01-18 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First volume gives an overview of the enzymes involved in DNA synthesis and modification; the second volume deals with the RNA-enzymes. Although the major emphasis of the book is on eukaryotic enzymes, a separate chapter dealing with prokaryotic DNA repair enzymes has been included to discuss the major advances in this field in recent years. There are two separate chapters on RNA polymerases to provide a comprehensive coverage of the enzymes from lower eukaryotes, plants and higher eukaryotes.
Author : Gretchen Ann Rice
Publisher :
ISBN 13 :
Total Pages : 248 pages
Book Rating : 4.:/5 (33 download)
Download or read book Analysis of RNA Polymerase II Ternary Complexes written by Gretchen Ann Rice and published by . This book was released on 1990 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Rodney Gerard Weilbaecher
Publisher :
ISBN 13 :
Total Pages : 536 pages
Book Rating : 4.:/5 (34 download)
Download or read book Characterization of an RNA Polymerase II Subunit, RPB9, and a Transcript Elongation Factor, TFIIS, from Saccharomyces Cerevisiae written by Rodney Gerard Weilbaecher and published by . This book was released on 1997 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sharon Lynn Wampler
Publisher :
ISBN 13 :
Total Pages : 170 pages
Book Rating : 4.:/5 (318 download)
Download or read book A Biochemical Analysis of Transcription Initiation by RNA Polymerase II written by Sharon Lynn Wampler and published by . This book was released on 1993 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Woo-Yeon Kim
Publisher :
ISBN 13 :
Total Pages : 294 pages
Book Rating : 4.:/5 (39 download)
Download or read book Mammalian RNA Polymerase IIO written by Woo-Yeon Kim and published by . This book was released on 1988 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Robert Marc Learned
Publisher :
ISBN 13 :
Total Pages : 316 pages
Book Rating : 4.:/5 (27 download)
Download or read book The Biochemical and Genetic Analysis of Human RRNA Transcription by RNA Polymerase I written by Robert Marc Learned and published by . This book was released on 1986 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mark E. Samuels
Publisher :
ISBN 13 :
Total Pages : 424 pages
Book Rating : 4.:/5 (132 download)
Download or read book Biochemical Studies of Transcription by RNA Polymerase II written by Mark E. Samuels and published by . This book was released on 1985 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jaesang Kim
Publisher :
ISBN 13 :
Total Pages : 320 pages
Book Rating : 4.:/5 (356 download)
Download or read book Isolation and Functional Characterization of Cofactors of RNA Polymerase II Transcription written by Jaesang Kim and published by . This book was released on 1996 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
