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Bioanalytical Applications Of Fluorescence Line Narrowing And Non Line Narrowing Spectroscopy Interfaced With Capillary Electrophoresis And High Performance Liquid Chromatography
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Book Synopsis Bioanalytical Applications of Fluorescence Line-narrowing and Non-line-narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-performance Liquid Chromatography by : Kenneth Paul Roberts
Download or read book Bioanalytical Applications of Fluorescence Line-narrowing and Non-line-narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-performance Liquid Chromatography written by Kenneth Paul Roberts and published by . This book was released on 2001 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: After cooldown the stationary analyte plugs can be interrogated indefinitely, which leads to significantly lower detection limits. Photodegradation of the analytes is also markedly reduced relative to laser excitation at room temperature. Automated translation of the cryostat/capillary relative to the spatially fixed laser excitation beam allows for sequential analysis of the separated analytes. Lower resolution but still informative analyses can be performed at 77 K. Results are presented for depurinating polycyclic aromatic hydrocarbons adducted to DNA isolated from the urine of humans exposed to coal smoke, and from the skin of mice treated with the most potent chemical carcinogen, dibenzo[a, l]pyrene. We anticipate that combining FLN and NLN detection with CE and HPLC will be useful in the many more bioanalytical applications.
Book Synopsis Bioanalytical Applications of Fluorescence Line-Narrowing and Non-Line-Narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-Performance Liquid Chromatography by :
Download or read book Bioanalytical Applications of Fluorescence Line-Narrowing and Non-Line-Narrowing Spectroscopy Interfaced with Capillary Electrophoresis and High-Performance Liquid Chromatography written by and published by . This book was released on 2001 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are widely used analytical separation techniques with many applications in chemical, biochemical, and biomedical sciences. Conventional analyte identification in these techniques is based on retention/migration times of standards; requiring a high degree of reproducibility, availability of reliable standards, and absence of coelution. From this, several new information-rich detection methods (also known as hyphenated techniques) are being explored that would be capable of providing unambiguous on-line identification of separating analytes in CE and HPLC. As further discussed, a number of such on-line detection methods have shown considerable success, including Raman, nuclear magnetic resonance (NMR), mass spectrometry (MS), and fluorescence line-narrowing spectroscopy (FLNS). In this thesis, the feasibility and potential of combining the highly sensitive and selective laser-based detection method of FLNS with analytical separation techniques are discussed and presented. A summary of previously demonstrated FLNS detection interfaced with chromatography and electrophoresis is given, and recent results from on-line FLNS detection in CE (CE-FLNS), and the new combination of HPLC-FLNS, are shown.
Book Synopsis Dissertation Abstracts International by :
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2001 with total page 790 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 2000 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Commencement by : Iowa State University
Download or read book Commencement written by Iowa State University and published by . This book was released on 2001 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Nanoscale Protein Analysis Utilizing Capillary Electrophoresis and Laser-induced Fluorescence Detection by : Michael D. Harvey
Download or read book Nanoscale Protein Analysis Utilizing Capillary Electrophoresis and Laser-induced Fluorescence Detection written by Michael D. Harvey and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The trend towards high throughput applications and miniaturization necessitates approaches capable of microlitre volume sampling and low protein concentration detection. Furthermore, one of the major trends in high throughput screening is the growing replacement of technologies that depend on radioactivity to generate a signal with those that rely on fluorescence. This trend towards non-radioactive detection in general can be understood by some of the advantages inherent to these methods over radioactive modes. These include a significant reduction in safety concerns leading to a relaxation of strict laboratory procedures, elimination of expensive waste disposal, extended shelf-life of labeled reagents, and the possibility of acquiring multiplexed data through the spectral isolation of different wavelength signals. A variety of capillary electrophoretic (CE) approaches utilizing laser-induced fluorescence (LIF) have thus been developed, providing researchers with valuable tools in protein analysis. Various covalent and non-covalent fluorescent derivatization approaches have been investigated, with emphasis on biochemical and/or clinical applications. The non-covalent dye, NanoOrange, is used as a clinical diagnostic tool for early disease diagnosis, quantitating nanomolar concentrations of human serum albumin in solution, and obtaining fluorescence-based biofluid profiles. An alternate non-covalent labeling approach utilizing the fluorescent probe, Sypro Red, and capillary gel electrophoresis allows for rapid, sensitive analysis of protein sample purity as well as molecular weight determination. These two non-covalent approaches are complemented by the development of a fluorescent Insulin-Like Growth Factor-I (IGF-I) analog for use in bioanalytical applications. Specific derivatization reaction conditions were developed to selectively label the N-terminus of the analog hence preserve biological activity. High-performance liquid chromatography and electrospray mass spectrometry were used to confirm the extent of labeling and modification site. Antibody recognition of this fluorescent analog was evaluated using CE-LIF, illustrating the clinical utility of this diagnostic reagent. In addition to the above CE-LIF approaches, a fourth capillary electrophoretic tool is provided for the clinical chemist. Rapid analysis of biofluids is of significant importance in early disease diagnosis. As such, an extensive CE-based analysis of human seminal plasma is presented. Separation conditions, sample stability, and protein/non-protein zone identification issues are addressed. This study and the CE-LIF methodologies discussed above represent original approaches to nanoscale protein analysis.
Book Synopsis Analytical Biotechnology by : Csaba Horváth
Download or read book Analytical Biotechnology written by Csaba Horváth and published by . This book was released on 1990 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents accounts of some of the recent advances in high performance liquid chromatography and capillary electrophoresis with regard to biotechnology. Four of its 11 chapters present an introduction to capillary electrophoresis and discuss its application to various analytical problems ranging from the analysis of cyclic nucleotides to quality control in the pharmaceutical industry. Subsequent chapters cover recent developments in HPLC, with emphasis on analysis of pharmaceutical proteins; the problems associated with the use of HPLC as a detection method in preparative chromatography; the use of mass spectrometry in the structure determination of peptides; and the use of the displacement mode of chromatography.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1160 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Nanochromatography and Nanocapillary Electrophoresis by : Imran Ali
Download or read book Nanochromatography and Nanocapillary Electrophoresis written by Imran Ali and published by John Wiley & Sons. This book was released on 2009-04-01 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detection of drugs at low concentration is required in a variety of biological and medical situations, in order to avoid harmful side effects posed by some drug residues. The book details the instrumentation, detection, and application of nano chromatography (that is, any chromatographic and capillary electrophoretic method dealing with the detection of a sample at nano gram per liter or lower) and capillary electrophoresis in the analyses of biological and environmental samples. Methods discussed include: Nano Gas Chromatography, Nano Capillary Electrophoresis, Nano Chiral Chromatography, Micellar Electrokinetic Chromatography, Supercritical Fluid Chromatography, and Nano High Performance Liquid Chromatography.
Download or read book Bioanalytical Separations written by and published by Elsevier. This book was released on 2003-09-03 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioanalytical Separations is volume 4 of the multi-volume series, Handbook of Analytical Separations, providing reviews of analytical separation methods and techniques used for the determination of analytes across a whole range of applications. The theme for this volume is bioanalysis, in this case specifically meaning the analysis of drugs and their metabolites in biological fluids. - Discusses new developments in instrumentation and methods of analyzing drugs and their metabolites in biological fluids - Provides guidance to the different methods, their relative value to the user, and the advantages and pitfalls of their use - Future trends are identified, in terms of the potential impact of new technologies
Book Synopsis Fluorescence Line Narrowing Spectrometry by : Michael Joseph McGlade
Download or read book Fluorescence Line Narrowing Spectrometry written by Michael Joseph McGlade and published by . This book was released on 1983 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Principles and Practice of Bioanalysis by : Richard F. Venn
Download or read book Principles and Practice of Bioanalysis written by Richard F. Venn and published by CRC Press. This book was released on 2008-02-25 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the seven years since the publication of Principles and Practice of Bioanalysis bioanalytical methods have remained the same, but their usage patterns have changed. This second edition of a bestseller provides an updated guide to the techniques used in developing and running ultra-trace analyses for drugs, metabolites, and other substance
Book Synopsis Standardization and Quality Assurance in Fluorescence Measurements II by : Ute Resch-Genger
Download or read book Standardization and Quality Assurance in Fluorescence Measurements II written by Ute Resch-Genger and published by Springer Science & Business Media. This book was released on 2008-08-15 with total page 566 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical chemists and materials scientists will find this a useful addition to their armory. The contributors have sought to highlight the present state of affairs in the validation and quality assurance of fluorescence measurements, as well as the need for future standards. Methods included range from steady-state fluorometry and microfluorometry, microscopy, and micro-array technology, to time-resolved fluorescence and fluorescence depolarization imaging techniques.
Book Synopsis Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics by : Rawi Ramautar
Download or read book Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics written by Rawi Ramautar and published by John Wiley & Sons. This book was released on 2022-10-03 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics A powerful and essential resource for researchers with an interest in CE-MS In Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications, a team of distinguished researchers delivers a comprehensive overview of bioanalytical capillary electrophoresis coupled to mass spectrometry (CE-MS). The book explains foundational principles, technology as well the strategies and techniques used in data analysis for metabolic and proteomic studies. It also provides a global overview of recent developments and advances for improving CE-MS sensitivity and reproducibility. An essential handbook for everyone performing metabolomic and proteomic analysis, the information provided here will assist researchers in tapping into the full potential of this technique to answer biological and clinical questions. Readers will also find: A thorough introduction to the principles of capillary electrophoresis, including its fundamentals, CE separation modes, capillary coatings, and the fundamentals of mass spectrometry In-depth examinations of technological developments in capillary electrophoresis, including sample preparation, online preconcentration, detection sensitivity, and metabolic coverage Comprehensive discussions of metabolomic studies, including their biomedical and clinical applications Recent advances in proteomics, including top-down and bottom-up approaches Perfect for analytical and clinical chemists, Capillary Electrophoresis—Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications will also earn a place in the libraries of biochemists, molecular biologists, and other molecular life scientists.
Download or read book Energy Research Abstracts written by and published by . This book was released on 1990 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Biochemical Applications by : Joseph R. Lakowicz
Download or read book Biochemical Applications written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial three volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. These first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).
Book Synopsis Modern Fluorescence Spectroscopy by : E. L. Wehry
Download or read book Modern Fluorescence Spectroscopy written by E. L. Wehry and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the appearance of the first two volumes of Modern Fluorescence Spectroscopy in 1976, important advances continue to be made in both the techniques and applications of molecular luminescence. In terms of "hardware," it is only recently that the application of laser excitation to molecular fluorometry has become feasible under conditions that are analy tically realistic. The improvements that can be effected in sensitivity, analy tical selectivity, and ability to handle "difficult" samples by laser fluorometry have only begun to be exploited. Likewise, time-resolved fluorometry has received widespread use in fundamental studies (a sizable number of which deal with biological systems), but has as of yet received relatively little analytical utilization. The use of electronic array detectors offers the promise of obtaining luminescence spectra more rapidly, and perhaps ultimately with greater sensitivity, than is possible by the use of scanning instruments equipped with conventional detectors. The increasing capabilities of microcomputers and the increasing sophistication of "smart" spectroscopic instrumentation signify that much more efficient acquisition and use can now be achieved of the information contained in the "excitation-emission matrix" inherent in the luminescence phenomenon.
