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Basic Dna And Rna Protocols
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Book Synopsis Basic DNA and RNA Protocols by : Adrian J. Harwood
Download or read book Basic DNA and RNA Protocols written by Adrian J. Harwood and published by . This book was released on 1996-02-19 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Basic DNA and RNA Protocols experienced researchers and innovators present an essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. Its outstanding, thoroughly up-to-date, and eminently practical protocols make it an essential tool for today's molecular biologist.
Book Synopsis Basic DNA and RNA Protocols by : Adrian J. Harwood
Download or read book Basic DNA and RNA Protocols written by Adrian J. Harwood and published by . This book was released on 2005-07-01 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods in Molecular Biology: Basic DNA and RNA protocols by : John M. Walker
Download or read book Methods in Molecular Biology: Basic DNA and RNA protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Nucleic Acid Protocols Handbook by : Ralph Rapley
Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Book Synopsis Current Protocols in Molecular Biology by :
Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis RNA Isolation and Characterization Protocols by : Ralph Rapley
Download or read book RNA Isolation and Characterization Protocols written by Ralph Rapley and published by Humana Press. This book was released on 2013-08-18 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.
Book Synopsis Techniques in Molecular Systematics and Evolution by : Rob DeSalle
Download or read book Techniques in Molecular Systematics and Evolution written by Rob DeSalle and published by Birkhäuser. This book was released on 2013-12-01 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the
Download or read book PCR Protocols written by Bruce A. White and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Book Synopsis Molecular Biology and Biotechnology by : M. P. Bansal
Download or read book Molecular Biology and Biotechnology written by M. P. Bansal and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.
Book Synopsis Molecular Techniques in Taxonomy by : Godfrey M. Hewitt
Download or read book Molecular Techniques in Taxonomy written by Godfrey M. Hewitt and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
Book Synopsis Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls by : John T. Corthell
Download or read book Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls written by John T. Corthell and published by Academic Press. This book was released on 2014-04-16 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules
Book Synopsis RNA Isolation and Characterization Protocols by : Ralph Rapley
Download or read book RNA Isolation and Characterization Protocols written by Ralph Rapley and published by Humana Press. This book was released on 1998-04-08 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.
Book Synopsis Protocols used in Molecular Biology by : Sandeep Singh
Download or read book Protocols used in Molecular Biology written by Sandeep Singh and published by Bentham Science Publishers. This book was released on 2020-01-23 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Book Synopsis Basic Techniques in Biochemistry, Microbiology and Molecular Biology by : Aakanchha Jain
Download or read book Basic Techniques in Biochemistry, Microbiology and Molecular Biology written by Aakanchha Jain and published by Humana. This book was released on 2021-03-14 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.
Book Synopsis Current Protocols in Nucleic Acid Chemistry by : Serge L. Beaucage
Download or read book Current Protocols in Nucleic Acid Chemistry written by Serge L. Beaucage and published by Current Protocols. This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.
Book Synopsis Transcription Factor Protocols by : Martin J. Tymms
Download or read book Transcription Factor Protocols written by Martin J. Tymms and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effort to sequence the human genome is now moving toward a c- clusion. As all of the protein coding sequences are described, an increasing emphasis will be placed on understanding gene function and regulation. One important aspect of this analysis is the study of how transcription factors re- late transcriptional initiation by RNA polymerase II, which is responsible for transcribing nuclear genes encoding messenger RNAs. The initiation of Class II transcription is dependent upon transcription factors binding to DNA e- ments that include the core or basal promoter elements, proximal promoter elements, and distal enhancer elements. General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation. This initiation process appears to be a crucial step in the modulation of mRNA levels in response to developmental and environmental signals. Transcription Factor Protocols provides step-by-step procedures for key techniques that have been developed to study DNA sequences and the protein factors that regulate the transcription of protein encoding genes. This volume is aimed at providing researchers in the field with the well-detailed protocols that have been the hallmark of previous volumes of the Methods in Molecular TM Biology series.
Book Synopsis Current Protocols in Molecular Biology by : Frederick M. Ausubel
Download or read book Current Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 1987 with total page 1320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
