Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Download Atomic Force Microscopy Study Of Collagen Assembly full books in PDF, epub, and Kindle. Read online Atomic Force Microscopy Study Of Collagen Assembly ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author : Chad A. Stoltz
Publisher :
ISBN 13 :
Total Pages : 98 pages
Book Rating : 4.:/5 (299 download)
Download or read book Atomic Force Microscopy Study of Collagen Assembly written by Chad A. Stoltz and published by . This book was released on 1999 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Marla Gale
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)
Download or read book Collagen Assembly as Examined by Atomic Force Microscopy written by Marla Gale and published by . This book was released on 1995 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of atomic force microscopy (AFM) to image the assembly of Type I collagen from monomers, through the various intermediates, to mature fibrils, has been demonstrated for the first time. These stages of collagen assembly were found to proceed by a stepwise process, which commenced with axially associated oligomers (height ~ 1 nm, lengths 450-1200 nm) and proceeded by lateral and axial association through intermediate microfibrils of 2-3 nm in diameter (with periodicity ~ 67 nm). Further assembly into larger microfibrils (>10,000 nm in length) that aggregated readily was observed. The end product of assembly observed in this study was a mature fibril, which demonstrated 4D-banding with a periodicity of 67 nm.
Download or read book Collagen Assembly as Examined by Atomic Force Microscopy written by and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Chuck Kaung Wen
Publisher :
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.:/5 (225 download)
Download or read book Atomic Force Microscopy Studies of Fibrous Long Spacing Type Collagen Aggregates Formed In-vitro: Self-assembly, Structure and Stability written by Chuck Kaung Wen and published by . This book was released on 2003 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Richard Kil
Publisher :
ISBN 13 : 9780494400777
Total Pages : 160 pages
Book Rating : 4.4/5 (7 download)
Download or read book Helical Collagen Fibrils Produced in Vitro written by Richard Kil and published by . This book was released on 2007 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the most ubiquitous protein in animals, collagen is a key protein of study in a number of fields including materials engineering, biotechnology, and regenerative medicine. A cornerstone of these studies is the self-assembly pathway of fibrils in vivo and in vitro. Although non-helical fibrils have dominated past work, helical fibrils have recently garnered more attention, though only in vivo. Here, it is demonstrated that helical fibrils can be reconstituted in vitro with high initial monomer concentrations above 0.75mg/mL, from acid-solubilized type I collagen extracted from calfskin. Simultaneous and warm starts were used in 0.025M phosphate buffer, at temperatures spanning 24--37°C, with final pH values of & sim;7.2. Characterization for the first time by atomic force microscopy reveals a right-handed helical structure, and that they are indistinguishable from in vivo helical fibrils. The self-assembly pathway is discussed based on the final morphologies revealed.
Author : Ming Sun
Publisher :
ISBN 13 : 9780494305089
Total Pages : 170 pages
Book Rating : 4.3/5 (5 download)
Download or read book Bridging the Hierarchical Structures of Collagen by Atomic Force Microscopy: From Nanoscale to Mesoscale written by Ming Sun and published by . This book was released on 2006 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most abundant structural protein in mammalian tissues, Type I Collagen monomer, a long rope of 300 nm in length and 1.5 nm in diameter, can self assemble into different three-dimensional structures with multiple functions as diverse as transparent cornea, tough tendon, and strong bone. Although the microscopic structure of the monomer and the macroscopic structures of some higher hierarchical assembled fibrils have been characterized during the past years, the formation of these higher hierarchical structures, and the emergence of their bioactivities on the nano-to-mesoscale, are still not so clear. In our work, AFM (atomic force microscopy) was applied in vitro, primarily as a imaging tool to investigate the self assembled protofibril patterns (bottom-up method), and also as a 'molecular broom' to create monomer bundle patterns under appropriate force (top-down method). We believe those unique discoveries in our lab will definitely cast light on the understanding of the in vivo self-assembly and related structure-property relationships of collagen, and provide a functional surface coating method for tissue engineering and cell study.
![Download Parallel Studies of in Vitro Native Collagen Fibrillogenesis [microform] : Cold Start, Warm Start and Dialysis Assembly Pathways Revealed by Atomic Force Microscopy PDF Online Free](https://books.google.com/books/content/images/frontcover/jvPqjgEACAAJ?fife=w200-h370)
Author : Mark Jeremy Sinyor
Publisher : National Library of Canada = Bibliothèque nationale du Canada
ISBN 13 : 9780612841567
Total Pages : 176 pages
Book Rating : 4.8/5 (415 download)
Download or read book Parallel Studies of in Vitro Native Collagen Fibrillogenesis [microform] : Cold Start, Warm Start and Dialysis Assembly Pathways Revealed by Atomic Force Microscopy written by Mark Jeremy Sinyor and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 2003 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Peter Fratzl
Publisher : Springer Science & Business Media
ISBN 13 : 0387739068
Total Pages : 516 pages
Book Rating : 4.3/5 (877 download)
Download or read book Collagen written by Peter Fratzl and published by Springer Science & Business Media. This book was released on 2008-05-10 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Not only does this book provide a comprehensive review of current research advances in collagen structure and mechanics, it also explores this biological macromolecule’s many applications in biomaterials and tissue engineering. Readers gain an understanding of the structure and mechanical behavior of type I collagen and collagen-based tissues in vertebrates across all length scales, from the molecular (nano) to the organ (macro) level.
Author : Peter Eaton
Publisher : OUP Oxford
ISBN 13 : 0191576670
Total Pages : 256 pages
Book Rating : 4.1/5 (915 download)
Download or read book Atomic Force Microscopy written by Peter Eaton and published by OUP Oxford. This book was released on 2010-03-25 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) is an amazing technique that allies a versatile methodology (that allows measurement of samples in liquid, vacuum or air) to imaging with unprecedented resolution. But it goes one step further than conventional microscopic techniques; it allows us to make measurements of magnetic, electrical or mechanical properties of the widest possible range of samples, with nanometre resolution. This book will demystify AFM for the reader, making it easy to understand, and to use. It is written by authors who together have more than 30 years experience in the design, construction, and use of AFMs and will explain why the microscopes are made the way they are, how they should be used, what data they can produce, and what can be done with the data. Illustrative examples from the physical sciences, materials science, life sciences, nanotechnology and industry demonstrate the different capabilities of the technique.
Author : MARLA. GALE
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (978 download)
Download or read book Collagen Assembly as Examined by Atomic Microscopy written by MARLA. GALE and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jennifer Lu Tran
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (133 download)
Download or read book Dynamics of Collagen Films and Starch Observed by Atomic Force Microscopy written by Jennifer Lu Tran and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Collagen is the most abundant protein in the human body. In vivo, collagen is spatially arranged as aligned fibers. In vitro, collagen can be self-assembled into higher order structures. These hierarchical collagen networks, however, lack the alignment found in natural tissues. Using mica as a template, aligned arrays of collagen can be created due to the aggregation of collagen along the crystallographic orientation of the muscovite substrate. Potassium ions, which disturb the interaction between collagen and mica, were used to control the pattern of collagen alignment to create unidirectional and bidirectional oriented films. This reversible synthesis of patterned films from randomly arranged collagen was observed in real-time via atomic force microscopy (AFM) in fluid. This study demonstrates the mobility and reorganization of collagen on surfaces and it preference for specific arrangements under varying environmental conditions. AFM was also used to monitor starch phosphorylation by alpha-glucan water dikinase (GWD).
Author : Naghmeh Rezaei
Publisher :
ISBN 13 :
Total Pages : 97 pages
Book Rating : 4.:/5 (112 download)
Download or read book Mechanical Studies of Single Collagen Molecules Using Imaging and Force Spectroscopy written by Naghmeh Rezaei and published by . This book was released on 2016 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: Collagen is a key component of the extracellular matrix and is the most abundant protein in vertebrates. Collagen is found in almost every connective tissue of the body including skin, bone, tendon, cartilage, arteries and cornea, where it plays a crucial role in providing structural support. Collagen molecules self-assemble to form hierarchical structures, from single molecules to fibrils to fibers and tissues. Structural and mechanical changes at the molecular level may affect self-assembly of the molecules and the resulting tissue. Despite its significance, the mechanics of collagen and its flexibility at the molecular level remain contentious, and collagen has been variously described as a flexible polymer to a semi-rigid rod. In this thesis, I present my work developing and utilizing experimental and analytical tools to study the mechanical proprieties of molecular collagen. I carefully designed and controlled a wide variety of experimental conditions, such as different collagen types and sources, solution pH and salt concentrations, and analysed the results in search of potential reasons for inconsistency in reported results of collagen flexibility at the basic molecular level. Atomic force microscopy (AFM) imaging is used to study effect of environmental factors such as ionic strength and pH on molecular conformations and flexibility of single collagen molecules. In addition, molecular conformations of different types of collagen from different sources are compared using AFM imaging. I measure persistence length of collagen molecules, a measure of flexibility, arising due to the conformational sampling of collagen. My results link the bending energy of collagen molecules to how tightly the helix is wound. In order to analyse AFM images of collagen, I developed an image and statistical analysis algorithm, SmarTrace, optimized for my images of collagen. The program was validated using images of DNA with known persistence length, then applied to collagen molecules. Analysis of different types of collagen in two different solutions and type I collagen in solutions of different ionic strength and pH show that collagen's flexibility depends strongly on ionic strength and pH. In addition, it shows that different types of collagen show similar average conformational characteristics in a given solution environment. In addition, mechanical properties and force-response of single collagen and procollagen molecules are studied using optical tweezers. I discuss the challenges of stretching single collagen proteins, whose length is much less than the size of the microspheres used as manipulation handles, and show how instrumental design and biochemistry can be used to overcome these challenges. The result of this work is an improved understanding of the sensitivity of molecular flexibility, stability and response of collagen to environmental factors. This can shed light on identifying underlying mechanisms of collagen-related diseases as well as designing and producing improved engineered biomaterials with tunable properties.
Author : Victor J Morris
Publisher : World Scientific
ISBN 13 : 190897821X
Total Pages : 423 pages
Book Rating : 4.9/5 (89 download)
Download or read book Atomic Force Microscopy For Biologists (2nd Edition) written by Victor J Morris and published by World Scientific. This book was released on 2009-08-11 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems.The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alike./a
Author : Ning Su
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (13 download)
Download or read book Atomic Force Microscopic, Electron Spectroscopic Imaging and Molecular Simulation Investigations of the Assembly and Structures of Collagen Constructs written by Ning Su and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jian Zhong
Publisher : Academic Press
ISBN 13 : 0128241322
Total Pages : 385 pages
Book Rating : 4.1/5 (282 download)
Download or read book Fundamentals and Application of Atomic Force Microscopy for Food Research written by Jian Zhong and published by Academic Press. This book was released on 2022-09-29 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals and Application of Atomic Force Microscopy for Food Research explains how to get reliable AFM data and current application progress of AFM in different food substances. Sections focus on an Introduction to AFM for food research and Applications of AFM for different types of food substances. Edited by 3 experts in the field of nanotechnology and food science, this book reduces the difficulty of AFM application and shortens the learning time for new hands. Until now, no such book has systematically described the application of Atomic Force Microscopy (AFM) for food research. Many scientists in the field of food science and engineering need to evaluate their developed foods and food contact surfaces at nanoscale. However, there is a steep learning curve for new hands, hence the need for this comprehensive resource. - Describes the application of AFM for food research - Covers applications of AFM for different types of food substances - Addresses future uses and perspectives of AFM for the development of food nanotechnology
Author :
Publisher : Academic Press
ISBN 13 : 008088816X
Total Pages : 562 pages
Book Rating : 4.0/5 (88 download)
Download or read book Introduction to Electron Microscopy for Biologists written by and published by Academic Press. This book was released on 2008-10-22 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
Author : Vinayak Mull
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (141 download)
Download or read book Development of Adhesion Force Microscopy for the Surface Charge Analysis of Collagen Fibrils in Vivo and in Vitro written by Vinayak Mull and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Collagen fibrils are a key component of the extracellular matrix. In this study, we use the adhesion force between a silicon probe and a collagen substrate to demonstrate the sensitivity of adhesion force towards charge distribution on the surface of collagen fibrils. We map the charge distribution at the surface of single in vivo and in vitro assembled collagen fibrils and characterize the location and strength of three sub-D-band regions that have been observed previously by cryo-electron microscopy. Our approach provides an adhesion fingerprint unique to each fibril type and points to local charge variations at the sub-D-band level even along a single fibril. We also demonstrate that this technique is capable of detecting changes on the surface of collagen fibrils because of glycation. It opens the road for a detailed analysis of collagen fibrils surface charge modifications due to ligand binding or mechanical strain on a fibril-by-fibril basis.
