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Atomic Force Microscopy Studies Of Protein Interactions With Lipid Membranes
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Book Synopsis Atomic Force Microscopy Studies of Protein Interactions with Lipid Membranes by : George Richard Heath
Download or read book Atomic Force Microscopy Studies of Protein Interactions with Lipid Membranes written by George Richard Heath and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Atomic Force Microscopy Studies of Protein Interactions with Lipid Membranes by : George Richard Heath
Download or read book Atomic Force Microscopy Studies of Protein Interactions with Lipid Membranes written by George Richard Heath and published by . This book was released on 2014 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Studies of Protein-protein Interactions at Membrane Interfaces Using Correlated Atomic Force and Fluorescence Microscopy by : James Eric Shaw
Download or read book Studies of Protein-protein Interactions at Membrane Interfaces Using Correlated Atomic Force and Fluorescence Microscopy written by James Eric Shaw and published by . This book was released on 2007 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Processes occurring at membrane interfaces are important for cellular and biological functions. The inability to readily crystallize membrane proteins for X-ray diffraction, or in solution NMR to study proteins with large molecular weight and to retain all intramolecular structures of the membrane proteins during their solubilisation process by detergents are the key reasons for a scarcity of atomic-resolution membrane protein structures. This has hindered our understanding of the structure-function relationship of membrane proteins. New strategies for studying membrane proteins will provide more insights. In this thesis, we have coupled atomic force microscopy and two fluorescence microscopy techniques---total internal reflectance fluorescence microscopy and confocal laser scanning microscopy, to visualize protein-membrane interactions and dynamics in model membrane systems. To validate our correlated approach, we investigated the partitioning of various lipid fluorescent probes in phase-separated supported planar bilayers. We applied the same methodology in two case studies of peptide-membrane interactions in supported planar bilayers: (1) indolicidin, a cationic 13 amino-acid peptide isolated from bovine neutrophils, as a model of antimicrobial peptide-membrane interactions, and (2) NAP-22 peptide, a cationic myristoylated peptide crucial for neuronal growth and plasticity, as a model of protein-induced lipid recruitment. Using this combined AFM-fluorescence approach with supported planar bilayers, we showed that: (1) the attachment of a fluorescent dye molecule can alter the physical properties of the host lipid and inhibit the lipid-dye conjugate from associating with raft domains, (2) indolicidin preferentially associates with the more disordered fluid domain and induces gel domain lowering in a concentration- and lipid-dependent manner, and (3) NAP-22 peptide induces coalescence of raft domains at low peptide concentration and phase-immersion at high peptide concentration resulting the co-localization of cholesterol and phosphatidylinositol biphosphates. This work demonstrates the versatility of a combined AFM-fluorescence approach as well as its limitations of the current configurations.
Book Synopsis Membrane Protein – Lipid Interactions: Physics and Chemistry in the Bilayer by : Jordi H. Borrell
Download or read book Membrane Protein – Lipid Interactions: Physics and Chemistry in the Bilayer written by Jordi H. Borrell and published by Springer. This book was released on 2016-03-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been conceives as a brief introduction to biomembranes physical chemistry for undergraduate students of sciences, and it is particularly dedicated to the lipid-protein membrane interactions. A general introduction is presented in Chapters 1 and 2. The following Chapters, 3 and 4, describe the most accepted theories on lipid-membrane protein interactions as well as the new experimental approaches, in particular, these arose from nano sciences as atomic for microscopy and single molecule force spectroscopy. The book emphasizes the relevance of physical parameters as the lateral surface pressure and the lipid curvature as actors for understanding the physicochemical properties of the biomembranes.
Download or read book Force Microscopy written by Bhanu P. Jena and published by John Wiley & Sons. This book was released on 2006-07-11 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: A complete examination of the uses of the atomic force microscope in biology and medicine This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function. The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers: * Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland * Probing the structural and physical properties of microbial cell surfaces * Scanning probe microscopic characterization of the higher plant cell wall and its components * Case studies of nano drug delivery systems using engineered dendrimers * AFM techniques for studying living cells * Investigating the intermolecular forces of leukocyte adhesion molecules * Protein-protein interactions * Micromechanical properties of lipid bilayers and vesicles The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine. This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.
Book Synopsis Atomic Force Microscopy For Biologists by : Patrick A Gunning
Download or read book Atomic Force Microscopy For Biologists written by Patrick A Gunning and published by World Scientific. This book was released on 1999-12-02 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems.
Book Synopsis Visualizing Protein Interactions at Supported Bilayer Surfaces by : Gillian Vanderlee
Download or read book Visualizing Protein Interactions at Supported Bilayer Surfaces written by Gillian Vanderlee and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Atomic Force Microscopy Investigations into Biology by : Christopher Frewin
Download or read book Atomic Force Microscopy Investigations into Biology written by Christopher Frewin and published by BoD – Books on Demand. This book was released on 2012-03-07 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The atomic force microscope (AFM) has become one of the leading nanoscale measurement techniques for materials science since its creation in the 1980's, but has been gaining popularity in a seemingly unrelated field of science: biology. The AFM naturally lends itself to investigating the topological surfaces of biological objects, from whole cells to protein particulates, and can also be used to determine physical properties such as Young's modulus, stiffness, molecular bond strength, surface friction, and many more. One of the most important reasons for the rise of biological AFM is that you can measure materials within a physiologically relevant environment (i.e. liquids). This book is a collection of works beginning with an introduction to the AFM along with techniques and methods of sample preparation. Then the book displays current research covering subjects ranging from nano-particulates, proteins, DNA, viruses, cellular structures, and the characterization of living cells.
Book Synopsis Protein-lipid Interactions in Raft-exhibiting Membranes Probed by Combined AFM and FCS. by :
Download or read book Protein-lipid Interactions in Raft-exhibiting Membranes Probed by Combined AFM and FCS. written by and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The cellular membrane is a complex biological entity, far from being an inert assembly of protein and lipids which separates cells from the surrounding environment. A multitude of biological processes, ranging from active transport of ions into and out of the cell, to the immune response, are regulated at the level of the plasma membrane. The understanding of their molecular basis is among the central goals of modern biological research. In order to dissect the complexity of actual cell membranes, which involves a very complex network of intermolecular interactions, a "divide and conquer" strategy proves very useful. To this end, researchers try to isolate molecules from complex biological contexts to understand their function in simple model systems under controlled conditions. A variety of model membranes have thus been developed in order to gain insight into membrane processes. This approach has resulted in a deeper knowledge on how lipids and proteins interact and how these interactions govern the function of cellular membranes. In the recent past in fact, a connection has been established between the lateral structure of the plasma membrane and its biological function. Furthermore, a large range of biophysical techniques have been used to characterize protein-lipid microdomains. For example, atomic force microscopy (AFM) is a powerful technique which allows a highly detailed topographical characterization of lipid domains in physiological conditions. While AFM imaging offers an extremely high spatial resolution, up to the nanometer scale, the limited image acquisition speed (~minutes) can pose a severe drawback in adequately studying fast dynamic processes. On the other hand, fluorescence based imaging techniques are much faster (~10-3-100 s), but certainly lack the high spatial resolution that AFM offers. FCS in particular can also provide information about dynamic processes, like diffusion of fluorescent membrane components. For these reasons, implementing a.
Book Synopsis Life - As a Matter of Fat by : Ole G. Mouritsen
Download or read book Life - As a Matter of Fat written by Ole G. Mouritsen and published by Springer Science & Business Media. This book was released on 2005-10-24 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a multi-disciplinary perspective on the physics of life and the particular role played by lipids and the lipid-bilayer component of cell membranes. Emphasizes the physical properties of lipid membranes seen as soft and molecularly structured interfaces. By combining and synthesizing insights obtained from a variety of recent studies, an attempt is made to clarify what membrane structure is and how it can be quantitatively described. Shows how biological function mediated by membranes is controlled by lipid membrane structure and organization on length scales ranging from the size of the individual molecule, across molecular assemblies of proteins and lipid domains in the range of nanometers, to the size of whole cells. Applications of lipids in nano-technology and biomedicine are also described.
Book Synopsis Membrane Biophysics by : Hongda Wang
Download or read book Membrane Biophysics written by Hongda Wang and published by Springer. This book was released on 2017-11-21 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights recent advances in and diverse techniques for exploring the plasma membrane’s structure and function. It starts with two chapters reviewing the history of membrane research and listing recent advances regarding membrane structure, such as the semi-mosaic model for red blood cell membranes and the protein layer-lipid-protein island model for nucleated tissue cell membranes. It subsequently focuses on the localization and interactions of membrane components, dynamic processes of membrane transport and transmembrane signal transduction. Classic and cutting-edge techniques (e.g. high-resolution atomic force microscopy and super-resolution fluorescence microscopy) used in biophysics and chemistry are presented in a very comprehensive manner, making them useful and accessible to both researchers in the field and novices studying cell membranes. This book provides readers a deeper understanding of the plasma membrane’s organization at the single molecule level and opens a new way to reveal the relationship between the membrane’s structure and functions, making it essential reading for researchers in various fields.
Book Synopsis Atomic Force Microscopy for Biologists by : V. J. Morris
Download or read book Atomic Force Microscopy for Biologists written by V. J. Morris and published by World Scientific Publishing Company. This book was released on 1999-01-01 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Macromolecules; Interfacial systems; ordered macromoleculles; Cells, tissue and biominerals; STM; SNOM; SICM; SThM; PFM.
Book Synopsis Probing Protein-lipid Interactions Using Atomic Force Microscopy by : Swetha Suresh
Download or read book Probing Protein-lipid Interactions Using Atomic Force Microscopy written by Swetha Suresh and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fundamentals and Application of Atomic Force Microscopy for Food Research by : Jian Zhong
Download or read book Fundamentals and Application of Atomic Force Microscopy for Food Research written by Jian Zhong and published by Academic Press. This book was released on 2022-09-29 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals and Application of Atomic Force Microscopy for Food Research explains how to get reliable AFM data and current application progress of AFM in different food substances. Sections focus on an Introduction to AFM for food research and Applications of AFM for different types of food substances. Edited by 3 experts in the field of nanotechnology and food science, this book reduces the difficulty of AFM application and shortens the learning time for new hands. Until now, no such book has systematically described the application of Atomic Force Microscopy (AFM) for food research. Many scientists in the field of food science and engineering need to evaluate their developed foods and food contact surfaces at nanoscale. However, there is a steep learning curve for new hands, hence the need for this comprehensive resource. - Describes the application of AFM for food research - Covers applications of AFM for different types of food substances - Addresses future uses and perspectives of AFM for the development of food nanotechnology
Author :Daniel Mark Czajkowsky Publisher :Ann Arbor, Mich. : University Microfilms International ISBN 13 : Total Pages :470 pages Book Rating :4.:/5 (458 download)
Book Synopsis Structural Studies of Membranes and Membrane Proteins with Atomic Force Microscopy by : Daniel Mark Czajkowsky
Download or read book Structural Studies of Membranes and Membrane Proteins with Atomic Force Microscopy written by Daniel Mark Czajkowsky and published by Ann Arbor, Mich. : University Microfilms International. This book was released on 2000 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Peptide-Lipid Interactions by : Sidney A. Simon
Download or read book Peptide-Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction