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Author : Li Zhu
Publisher : Eaton Publishing Company/Biotechniques Books
ISBN 13 :
Total Pages : 166 pages
Book Rating : 4.:/5 (89 download)
Download or read book Apoptosis Detection and Assay Methods written by Li Zhu and published by Eaton Publishing Company/Biotechniques Books. This book was released on 1998 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Gil Mor
Publisher : Springer Science & Business Media
ISBN 13 : 1588294579
Total Pages : 271 pages
Book Rating : 4.5/5 (882 download)
Download or read book Apoptosis and Cancer written by Gil Mor and published by Springer Science & Business Media. This book was released on 2007-10-11 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
Author : John C. Reed
Publisher : Elsevier
ISBN 13 : 0121822230
Total Pages : 604 pages
Book Rating : 4.1/5 (218 download)
Download or read book Apoptosis written by John C. Reed and published by Elsevier. This book was released on 2000-07-05 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Author : Hugh J. M. Brady
Publisher : Springer Science & Business Media
ISBN 13 : 1592598129
Total Pages : 306 pages
Book Rating : 4.5/5 (925 download)
Download or read book Apoptosis Methods and Protocols written by Hugh J. M. Brady and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most fundamental question facing each and every cell within an org- ism is to survive or to die. Cell death is required for normal function; some estimates suggest that as many as one million cells undergo cell death every second in the adult human body. Almost all cells undergoing physiological, or programmed, cell death, independent of cell type, manifest a stereotypic p- tern of morphological changes termed apoptosis. Typically, apoptotic cells d- play shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. The integrity of the cell membrane is not lost during apoptosis and so avoids eliciting the inflammatory response that would have been caused by the spillage of the cell’s contents. This is quite in contrast to the loss of cell contents typical of necrosis. The caspases, the family of intracellular cysteine proteases associated with apoptosis, are responsible for the stereotypical m- phological changes. Caspases cleave various substrate proteins that act on DNA fragmentation, nuclear envelope integrity, the cytoskeleton, and cell volume regulation. Apoptotic cells are cleared in vivo by the process of phagocytosis, in which specific “phagocytes” move to the site of apoptosis, engulf the dying cells and digest them. Apoptosis has a central role in many physiological processes, for example, in the immune system. Autoreactive cells are deleted via apoptosis to prevent autoimmunity. At the end of an immune response, activated lymphocytes are removed to maintain homeostasis within the immune system.
Author : Kim H. Wong
Publisher :
ISBN 13 :
Total Pages : 258 pages
Book Rating : 4.:/5 (843 download)
Download or read book Exploring Alternative Methods to Improve the Detection of Apoptosis in a Solution-based Assay written by Kim H. Wong and published by . This book was released on 2011 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Rosalyn D. Blumenthal
Publisher : Springer Science & Business Media
ISBN 13 : 1592598692
Total Pages : 234 pages
Book Rating : 4.5/5 (925 download)
Download or read book Chemosensitivity written by Rosalyn D. Blumenthal and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
Author : George P. Patrinos
Publisher : Academic Press
ISBN 13 : 0128029889
Total Pages : 526 pages
Book Rating : 4.1/5 (28 download)
Download or read book Molecular Diagnostics written by George P. Patrinos and published by Academic Press. This book was released on 2016-10-27 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field
Author : P. Erhard
Publisher : Humana Press
ISBN 13 : 9781617794896
Total Pages : 0 pages
Book Rating : 4.7/5 (948 download)
Download or read book Apoptosis written by P. Erhard and published by Humana Press. This book was released on 2011-12-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.
Author : David Hughes
Publisher : Taylor & Francis
ISBN 13 : 1135323216
Total Pages : 403 pages
Book Rating : 4.1/5 (353 download)
Download or read book Cell Proliferation and Apoptosis written by David Hughes and published by Taylor & Francis. This book was released on 2004-03 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Proliferation and Apoptosis provides a detailed practical guide to cell proliferation and apoptosis detection methods. A novel approach combining both these areas allows important comparisons to be made. Topics covered include all aspects of tissue handling from collection, storage, fixation and processing through to locating and quantifying cells in different stages of the cell cycle. This book is an essential and comprehensive practical guide to these important and expanding areas.
Author : Vladimir V. Didenko
Publisher : Humana Press
ISBN 13 : 9781603274081
Total Pages : 0 pages
Book Rating : 4.2/5 (74 download)
Download or read book DNA Damage Detection In Situ, Ex Vivo, and In Vivo written by Vladimir V. Didenko and published by Humana Press. This book was released on 2010-11-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.
Author : John M. S. Bartlett
Publisher : Springer Science & Business Media
ISBN 13 : 1592590713
Total Pages : 787 pages
Book Rating : 4.5/5 (925 download)
Download or read book Ovarian Cancer written by John M. S. Bartlett and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt: If there is one aspect of current cancer research that represents a major ch- lenge in both novice and experienced researchers, it is the rapid advance in our understanding of the disease. Researchers can be required to switch from analysis of gene expression to kinetics of protein activation, from genetic studies to the analysis of protein funtion. Cancers are highly complex disease systems and researchers aiming to understand the functioning of cancer systems require access to a wide range of laboratory techiques from a broad range of research disciplines. Increasingly, however, published methods are incomplete or refer back to a series of previous publications each containing only a small part of the complete pro- col. The aim of Ovarian Cancer: Methods and Protocols is to provide for ovarian cancer researchers in the first instance, a laboratory handbook that will facilitate research into cancer systems by providing a series of expert protocols, with proven efficacy, across a broad range of technical expertise. Thus, there are sections on tumor genetics and cellular signal transduction, as well as sections on apoptosis and RNA analysis. The value of Ovarian Cancer: Methods and Protocols to the ovarian cancer researcher will, I trust, be considerably enhanced by (1) the provision of a series of overviews relating to the biology, diagnosis, and treatment of this important neoplasm, and (2) the provision of a series of technical overviews introducing each part that provides an expert review of the applications and pitfalls of the various techniques included.
Author : Erman Salih Istifli
Publisher : BoD – Books on Demand
ISBN 13 : 1789847540
Total Pages : 120 pages
Book Rating : 4.7/5 (898 download)
Download or read book Cytotoxicity written by Erman Salih Istifli and published by BoD – Books on Demand. This book was released on 2019-10-02 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Compensating for cytotoxicity in the multicellular organism by a certain level of cellular proliferation is the primary aim of homeostasis. In addition, the loss of cellular proliferation control (tumorigenesis) is at least as important as cytotoxicity, however, it is a contrasting trauma. With the disruption of the delicate balance between cytotoxicity and proliferation, confrontation with cancer can inevitably occur. This book presents important information pertaining to the molecular control of the mechanisms of cytotoxicity and cellular proliferation as they relate to cancer. It is designed for students and researchers studying cytotoxicity and its control.
Author : Perpetua M. Muganda
Publisher : Methods in Pharmacology and To
ISBN 13 : 9781493980864
Total Pages : 256 pages
Book Rating : 4.9/5 (88 download)
Download or read book Apoptosis Methods in Toxicology written by Perpetua M. Muganda and published by Methods in Pharmacology and To. This book was released on 2018-04-28 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Haian Fu
Publisher : Cambridge University Press
ISBN 13 : 1107377587
Total Pages : 359 pages
Book Rating : 4.1/5 (73 download)
Download or read book Chemical Genomics written by Haian Fu and published by Cambridge University Press. This book was released on 2012-02-13 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in chemistry, biology and genomics coupled with laboratory automation and computational technologies have led to the rapid emergence of the multidisciplinary field of chemical genomics. This edited text, with contributions from experts in the field, discusses the new techniques and applications that help further the study of chemical genomics. The beginning chapters provide an overview of the basic principles of chemical biology and chemical genomics. This is followed by a technical section that describes the sources of small-molecule chemicals; the basics of high-throughput screening technologies; and various bioassays for biochemical-, cellular- and organism-based screens. The final chapters connect the chemical genomics field with personalized medicine and the druggable genome for future discovery of new therapeutics. This book will be valuable to researchers, professionals and graduate students in many fields, including biology, biomedicine and chemistry.
Author : Shiqi Peng
Publisher : John Wiley & Sons
ISBN 13 : 0470522194
Total Pages : 523 pages
Book Rating : 4.4/5 (75 download)
Download or read book Pharmaceutical Bioassays written by Shiqi Peng and published by John Wiley & Sons. This book was released on 2009-12-09 with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive compendium of bioassay procedures and applications A virtual encyclopedia of key bioassay protocols, this up-to-date, essential resource reviews the methods and applications of bioassays that quantify drug activity and evaluate the validity of pharmacological models. Demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules, the book covers the evaluation and screening of drug compounds in a wide spectrum of therapeutic categories. Throughout, the authors use various models to link experimental observations with findings that clarify the effect of drug compounds on cellular biology and measure the pharmacological activity of chemical substances. This provides an important technological platform for successful drug research and clinical therapy, making Pharmaceutical Bioassays an ideal sourcebook for pharmacologists, pharmaceutical and medical researchers, analytical chemists, toxicologists, and students. Just as a fundamental knowledge of pharmaceutical bioassays is vital to understanding how bioactive agents and new drugs are defined, discovered, and developed, so too is this indispensable reference, which: Offers a complete reference on pharmaceutical bioassays and their specific applications in the assessment of therapies for cancer, Alzheimer’s, diabetes, epilepsy, inflammation, antimalarials, pharmacogenomics, and other major therapeutic areas Covers bioassays that measure toxicity, including those for chemical toxicity and hepatoxicity—a particularly significant consideration in the development of safe and tolerable medications Highlights clinically relevant methods, ideas, and techniques to support any pharmaceutical researcher Suggests guidelines and systems to enhance communication between pharmacologists, chemists, and biologists Provides an informative analysis of pharmaceutical bioassay technologies and lab applications
Author : T. G. Cotter
Publisher : Ashgate Publishing
ISBN 13 :
Total Pages : 352 pages
Book Rating : 4.3/5 (91 download)
Download or read book Techniques in Apoptosis written by T. G. Cotter and published by Ashgate Publishing. This book was released on 1996 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Discoveries in the past 2-3 years have highlighted the importance of cell death in numerous physiological and pathological mechanisms, making the study of this process one of the hottest topics in the biological sciences. This book will appeal to those who are new to the field or are thinking of entering the field and cannot afford to waste valuable time using outdated methods. It will also be of interest to those who are already working in the area but may not be aware of the multitude of methods that are now available for the assessment of apoptosis - as well as the pitfalls associated with a particular method. Techniques in Apoptosis is an essential bench companion for graduates, postdoctoral fellows, research scientists and clinicians working on any aspect of cell growth and cell death."--BOOK JACKET.Title Summary field provided by Blackwell North America, Inc. All Rights Reserved
Author : G. Warnes
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (115 download)
Download or read book The Multiplexing of Assays for the Measurement of Early Stages of Apoptosis by Polychromatic Flow Cytometry written by G. Warnes and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection of apoptosis has been a stalwart application for flow cytometric analysis for decades and this review of flow cytometric methods to detect early stages of apoptosis includes the use of the pivotal assay to detect early and late apoptosis, the Annexin V assay which when multiplexed with biologically functional fluorescent dyes to measure mitochondrial function and Reactive Oxygen Species (ROS) generation allows further identification of functionally different subsets within apoptotic populations. Here we show how this polychromatic approach can be used to demonstrate which subset of cells show changes in mitochondrial function and when ROS is generated in a time dependent manner. This polychromatic approach to flow cytometry leads to the identification of over ten sub-populations of cells during classic apoptosis or programmed cell death (PCD).
