Handbook of Single Molecule Fluorescence Spectroscopy

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Publisher : Oxford University Press on Demand
ISBN 13 : 0198529422
Total Pages : 279 pages
Book Rating : 4.1/5 (985 download)

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Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by Oxford University Press on Demand. This book was released on 2006-08-17 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Single Molecule Analysis

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Publisher : Springer Nature
ISBN 13 : 1071633775
Total Pages : 511 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Single Molecule Analysis by : Iddo Heller

Download or read book Single Molecule Analysis written by Iddo Heller and published by Springer Nature. This book was released on 2023-11-13 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Single Molecule Spectroscopy

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Publisher : Springer Science & Business Media
ISBN 13 : 3642565441
Total Pages : 375 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Single Molecule Spectroscopy by : R. Rigler

Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.

Handbook of Single Molecule Fluorescence Spectroscopy

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Publisher :
ISBN 13 : 9781383024876
Total Pages : 0 pages
Book Rating : 4.0/5 (248 download)

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Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Handbook of Fluorescence Spectroscopy and Imaging

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Publisher : John Wiley & Sons
ISBN 13 : 3527633529
Total Pages : 425 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Handbook of Fluorescence Spectroscopy and Imaging by : Markus Sauer

Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Single Molecule Tools, Part A: Fluorescence Based Approaches

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Publisher : Academic Press
ISBN 13 : 9780080959276
Total Pages : 528 pages
Book Rating : 4.9/5 (592 download)

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Book Synopsis Single Molecule Tools, Part A: Fluorescence Based Approaches by :

Download or read book Single Molecule Tools, Part A: Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Fluorescence Spectroscopy in Biology

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Publisher : Springer Science & Business Media
ISBN 13 : 9783540223382
Total Pages : 330 pages
Book Rating : 4.2/5 (233 download)

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Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof

Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2004-12-08 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Spectroscopy and Dynamics of Single Molecules

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Publisher : Elsevier
ISBN 13 : 0128164646
Total Pages : 402 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Spectroscopy and Dynamics of Single Molecules by :

Download or read book Spectroscopy and Dynamics of Single Molecules written by and published by Elsevier. This book was released on 2019-08-14 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction

Handbook of Single Molecule Fluorescence Spectroscopy

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Author :
Publisher : OUP Oxford
ISBN 13 : 0191523852
Total Pages : 278 pages
Book Rating : 4.1/5 (915 download)

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Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Fluorescence Spectroscopy

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Author :
Publisher : Academic Press
ISBN 13 : 9780080923345
Total Pages : 400 pages
Book Rating : 4.9/5 (233 download)

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Book Synopsis Fluorescence Spectroscopy by :

Download or read book Fluorescence Spectroscopy written by and published by Academic Press. This book was released on 2011-09-06 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics. * Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique. * Provides tried and tested techniques which eliminate searching through many different sources.

Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein

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Publisher : Springer
ISBN 13 : 3319095587
Total Pages : 59 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein by : Sharona Cohen

Download or read book Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein written by Sharona Cohen and published by Springer. This book was released on 2015-10-17 with total page 59 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis single-molecule fluorescence resonance energy transfer (FRET) spectroscopy was used to study the folding of a protein that belongs to the large and important family of repeat proteins. Cohen shows that the dynamics of the expanded conformations is likely to be very fast, suggesting a spring-like motion of the whole chain. The findings shed new light on the elasticity of structure in repeat proteins, which is related to their function in binding multiple and disparate partners. This concise research summary provides useful insights for students beginning a PhD in this or a related area, and researchers entering this field.

Single-molecule Techniques

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Publisher : CSHL Press
ISBN 13 : 087969775X
Total Pages : 511 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Single-molecule Techniques by : Paul R. Selvin

Download or read book Single-molecule Techniques written by Paul R. Selvin and published by CSHL Press. This book was released on 2008 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.

Spectroscopic Methods of Analysis

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Publisher : Humana Press
ISBN 13 : 9781493961412
Total Pages : 397 pages
Book Rating : 4.9/5 (614 download)

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Book Synopsis Spectroscopic Methods of Analysis by : Wlodek M. Bujalowski

Download or read book Spectroscopic Methods of Analysis written by Wlodek M. Bujalowski and published by Humana Press. This book was released on 2016-08-23 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, Spectroscopic Methods of Analysis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spectroscopic Methods of Analysis : Methods and Protocols seeks to aid scientists in the further study of optical spectroscopic methods.

Protein Conformational Dynamics

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Publisher : Springer Science & Business Media
ISBN 13 : 3319029703
Total Pages : 488 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Protein Conformational Dynamics by : Ke-li Han

Download or read book Protein Conformational Dynamics written by Ke-li Han and published by Springer Science & Business Media. This book was released on 2014-01-20 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.

Single-Molecule Optical Detection, Imaging and Spectroscopy

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Publisher : John Wiley & Sons
ISBN 13 : 3527614702
Total Pages : 264 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Single-Molecule Optical Detection, Imaging and Spectroscopy by : W. E. Moerner

Download or read book Single-Molecule Optical Detection, Imaging and Spectroscopy written by W. E. Moerner and published by John Wiley & Sons. This book was released on 2008-09-26 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single Molecule Spectroscopy is one of the hottest topics in today's chemistry. It brings us close to the the most exciting vision generations of chemists have been dreaming of: To observe and examine single molecules! While most of chemistry deals with myriads of molecules, this books presents the latest developments for the detection and investigation of single entities. Written by internationally renowned authors, it is a thorough and comprehensive survey of current methods and their applications.

Single Molecule Analysis

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Publisher : Humana Press
ISBN 13 : 9781617792816
Total Pages : 317 pages
Book Rating : 4.7/5 (928 download)

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Book Synopsis Single Molecule Analysis by : Erwin J. G. Peterman

Download or read book Single Molecule Analysis written by Erwin J. G. Peterman and published by Humana Press. This book was released on 2011-09-15 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

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Author :
Publisher : Academic Press
ISBN 13 : 0128095474
Total Pages : 616 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods by :

Download or read book Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods written by and published by Academic Press. This book was released on 2016-10-28 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion