Amino Acid Analysis

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Amino Acid Analysis

Author : Michail A. Alterman
Publisher : Humana Press
ISBN 13 : 9781617794445
Total Pages : 363 pages
Book Rating : 4.7/5 (944 download)

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Book Synopsis Amino Acid Analysis by : Michail A. Alterman

Download or read book Amino Acid Analysis written by Michail A. Alterman and published by Humana Press. This book was released on 2011-12-02 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Amino Acid Analysis Protocols

Author : Catherine Cooper
Publisher : Humana
ISBN 13 : 9780896036567
Total Pages : 265 pages
Book Rating : 4.0/5 (365 download)

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Book Synopsis Amino Acid Analysis Protocols by : Catherine Cooper

Download or read book Amino Acid Analysis Protocols written by Catherine Cooper and published by Humana. This book was released on 2000-09-05 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Protein Structure Analysis

Author : Roza Maria Kamp
Publisher : Springer Science & Business Media
ISBN 13 : 3642592198
Total Pages : 311 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Protein Structure Analysis by : Roza Maria Kamp

Download or read book Protein Structure Analysis written by Roza Maria Kamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 311 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Techniques in Protein Chemistry III

Author : Ruth Hogue Angeletti
Publisher : Academic Press
ISBN 13 : 1483259463
Total Pages : 563 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Techniques in Protein Chemistry III by : Ruth Hogue Angeletti

Download or read book Techniques in Protein Chemistry III written by Ruth Hogue Angeletti and published by Academic Press. This book was released on 2014-06-28 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.

Analytical Microbiology Methods

Author : A. Fox
Publisher : Springer Science & Business Media
ISBN 13 : 1489935649
Total Pages : 278 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Analytical Microbiology Methods by : A. Fox

Download or read book Analytical Microbiology Methods written by A. Fox and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First International Symposium on the Interface between Analytical Chemistry and Microbiology: Applications of Chromatography and Mass Spectrometry was held June 1987 at the University of South Carolina, Columbia, SC, U.S.A. The purpose of the "Interface" meeting was to forge connections between analytical chemists and microbiologists that are using chromatography and mass spectrometry to solve common problems. The goals were admirably fulfilled. Nearly a hundred participants from seven European countries, Japan, and the United States participated in hearing twenty-three plenary talks and thirty-six submitted papers and posters. The papers and discussions displayed the breadth and depth of current research applications and revealed future directions. This book "Analytical Microbiology Methods: Chromatography and Mass Spectrometry" is loosely based on some of the presentations and discussions at the meeting. Each chapter describes specific methodology and applications in the context of the relevant scientific background. The present book continues the theme of an earlier book, "Gas Chromatography/Mass Spectrometry Applications in Microbiology", edited by G. Odham, L. Larsson, and P-A. Mardh, published by Plenum Press in 1984.

Laboratory Guide to the Methods in Biochemical Genetics

Author : Nenad Blau
Publisher : Springer Science & Business Media
ISBN 13 : 3540766987
Total Pages : 860 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Laboratory Guide to the Methods in Biochemical Genetics by : Nenad Blau

Download or read book Laboratory Guide to the Methods in Biochemical Genetics written by Nenad Blau and published by Springer Science & Business Media. This book was released on 2008-05-31 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory.

Vademecum Metabolicum

Author : Johannes Zschocke
Publisher : Schattauer Verlag
ISBN 13 : 3794528166
Total Pages : 188 pages
Book Rating : 4.7/5 (945 download)

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Book Synopsis Vademecum Metabolicum by : Johannes Zschocke

Download or read book Vademecum Metabolicum written by Johannes Zschocke and published by Schattauer Verlag. This book was released on 2011 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Bioinformatics for Geneticists

Author : Michael R. Barnes
Publisher : John Wiley & Sons
ISBN 13 : 047086219X
Total Pages : 432 pages
Book Rating : 4.4/5 (78 download)

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Book Synopsis Bioinformatics for Geneticists by : Michael R. Barnes

Download or read book Bioinformatics for Geneticists written by Michael R. Barnes and published by John Wiley & Sons. This book was released on 2003-07-01 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: This timely book illustrates the value of bioinformatics, not simply as a set of tools but rather as a science increasingly essential to navigate and manage the host of information generated by genomics and the availability of completely sequenced genomes. Bioinformatics can be used at all stages of genetics research: to improve study design, to assist in candidate gene identification, to aid data interpretation and management and to shed light on the molecular pathology of disease-causing mutations. Written specifically for geneticists, this book explains the relevance of bioinformatics showing how it may be used to enhance genetic data mining and markedly improve genetic analysis.

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Contemporary Practice in Clinical Chemistry

Author : William Clarke
Publisher : Academic Press
ISBN 13 : 0128158336
Total Pages : 1070 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Contemporary Practice in Clinical Chemistry by : William Clarke

Download or read book Contemporary Practice in Clinical Chemistry written by William Clarke and published by Academic Press. This book was released on 2020-06-11 with total page 1070 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contemporary Practice in Clinical Chemistry, Fourth Edition, provides a clear and concise overview of important topics in the field. This new edition is useful for students, residents and fellows in clinical chemistry and pathology, presenting an introduction and overview of the field to assist readers as they in review and prepare for board certification examinations. For new medical technologists, the book provides context for understanding the clinical utility of tests that they perform or use in other areas in the clinical laboratory. For experienced laboratorians, this revision continues to provide an opportunity for exposure to more recent trends and developments in clinical chemistry. - Includes enhanced illustration and new and revised color figures - Provides improved self-assessment questions and end-of-chapter assessment questions

Chemistry and Biochemistry of the Amino Acids

Author : Graham Barrett
Publisher : Springer Science & Business Media
ISBN 13 : 9400948328
Total Pages : 694 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Chemistry and Biochemistry of the Amino Acids by : Graham Barrett

Download or read book Chemistry and Biochemistry of the Amino Acids written by Graham Barrett and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amino acids are featured in course syllabuses and in project and research work over a wide spectrum of subject areas in chemistry and biology. Chemists and biochemists using amino acids have many common needs when they turn to the literature for comprehensive information. Among these common interests, analytical studies, in particular, have undergone rapid development in recent years. All other chemical and biochemical aspects of amino acids - synthesis, properties and reactions, preparation of derivatives for use in peptide synthesis, racemization and other fundamental mechanistic knowledge - have been the subject of vigorous progress. This book offers a thorough treatment of all these developing areas, and is structured in the belief that biochemists, physiologists and others will profit from access to information on topics such as the physical chemistry of amino acid solutions, as well as from thorough coverage of amino acid metabolism, biosynthesis and enzyme inhibition; and that chemists will find relevant material in biological areas as well as in the analysis, synthesis and reactions of amino acids.

Basic Principles of Organic Chemistry

Author : John D. Roberts
Publisher :
ISBN 13 : 9780805383218
Total Pages : 1596 pages
Book Rating : 4.3/5 (832 download)

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Book Synopsis Basic Principles of Organic Chemistry by : John D. Roberts

Download or read book Basic Principles of Organic Chemistry written by John D. Roberts and published by . This book was released on 1977 with total page 1596 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction what is organic chemistry all about?; Structural organic chemistry the shapes of molecules functional groups; Organic nomenclature; Alkanes; Stereoisomerism of organic molecules; Bonding in organic molecules atomic-orbital models; More on nomenclature compounds other than hydrocarbons; Nucleophilic substitution and elimination reactions; Separation and purification identification of organic compounds by spectroscopic techniques; Alkenes and alkynes. Ionic and radical addition reactions; Alkenes and alkynes; Oxidation and reduction reactions; Acidity or alkynes.

Chemistry and Safety of Acrylamide in Food

Author : Mendel Friedman
Publisher : Springer Science & Business Media
ISBN 13 : 9780387239200
Total Pages : 492 pages
Book Rating : 4.2/5 (392 download)

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Book Synopsis Chemistry and Safety of Acrylamide in Food by : Mendel Friedman

Download or read book Chemistry and Safety of Acrylamide in Food written by Mendel Friedman and published by Springer Science & Business Media. This book was released on 2005-04-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interest in the chemistry, biochemistry, and safety of acrylamide is running high. These proceedings contain presentations by experts from eight countries on the chemistry, analysis, metabolism, pharmacology, and toxicology of the compound.

The Role of Protein and Amino Acids in Sustaining and Enhancing Performance

Author : Institute of Medicine
Publisher : National Academies Press
ISBN 13 : 0309172810
Total Pages : 448 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis The Role of Protein and Amino Acids in Sustaining and Enhancing Performance by : Institute of Medicine

Download or read book The Role of Protein and Amino Acids in Sustaining and Enhancing Performance written by Institute of Medicine and published by National Academies Press. This book was released on 1999-09-15 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a commonly held belief that athletes, particularly body builders, have greater requirements for dietary protein than sedentary individuals. However, the evidence in support of this contention is controversial. This book is the latest in a series of publications designed to inform both civilian and military scientists and personnel about issues related to nutrition and military service. Among the many other stressors they experience, soldiers face unique nutritional demands during combat. Of particular concern is the role that dietary protein might play in controlling muscle mass and strength, response to injury and infection, and cognitive performance. The first part of the book contains the committee's summary of the workshop, responses to the Army's questions, conclusions, and recommendations. The remainder of the book contains papers contributed by speakers at the workshop on such topics as, the effects of aging and hormones on regulation of muscle mass and function, alterations in protein metabolism due to the stress of injury or infection, the role of individual amino acids, the components of proteins, as neurotransmitters, hormones, and modulators of various physiological processes, and the efficacy and safety considerations associated with dietary supplements aimed at enhancing performance.

Quantitative Methods in Proteomics

Author : Katrin Marcus
Publisher : Humana Press
ISBN 13 : 9781617798849
Total Pages : 539 pages
Book Rating : 4.7/5 (988 download)

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Book Synopsis Quantitative Methods in Proteomics by : Katrin Marcus

Download or read book Quantitative Methods in Proteomics written by Katrin Marcus and published by Humana Press. This book was released on 2012-06-08 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein modifications and changes made to them, as well as the quantities of expressed proteins, can define the various functional stages of the cell. Accordingly, perturbations can lead to various diseases and disorders. As a result, it has become paramount to be able to detect and monitor post-translational modifications and to measure the abundance of proteins within the cell with extreme sensitivity. While protein identification is an almost routine requirement nowadays, reliable techniques for quantifying unmodified proteins (including those that escape detection under standard conditions, such as protein isoforms and membrane proteins) is not routine. Quantitative Methods in Proteomics gives a detailed survey of topics and methods on the principles underlying modern protein analysis, from statistical issues when planning proteomics experiments, to gel-based and mass spectrometry-based applications. The quantification of post-translational modifications is also addressed, followed by the “hot” topics of software and data analysis, as well as various overview chapters which provide a comprehensive overview of existing methods in quantitative proteomics. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Quantitative Methods in Proteomics serves as a comprehensive and competent overview of the important and still growing field of quantitative proteomics.

Amino Acids, Peptides and Proteins in Organic Chemistry, Analysis and Function of Amino Acids and Peptides

Author :
Publisher : John Wiley & Sons
ISBN 13 : 3527631852
Total Pages : 508 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Amino Acids, Peptides and Proteins in Organic Chemistry, Analysis and Function of Amino Acids and Peptides by :

Download or read book Amino Acids, Peptides and Proteins in Organic Chemistry, Analysis and Function of Amino Acids and Peptides written by and published by John Wiley & Sons. This book was released on 2013-02-13 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the last of five books in the Amino Acids, Peptidesand Proteins in Organic Synthesis series. Closing a gap in the literature, this is the only series tocover this important topic in organic and biochemistry. Drawingupon the combined expertise of the international "who's who" inamino acid research, these volumes represent a real benchmark foramino acid chemistry, providing a comprehensive discussion of theoccurrence, uses and applications of amino acids and, by extension,their polymeric forms, peptides and proteins. The practical value of each volume is heightened by theinclusion of experimental procedures. The 5 volumes cover the following topics: Volume 1: Origins and Synthesis of Amino Acids Volume 2: Modified Amino Acids, Organocatalysis and Enzymes Volume 3: Building Blocks, Catalysis and Coupling Chemistry Volume 4: Protection Reactions, Medicinal Chemistry,Combinatorial Synthesis Volume 5: Analysis and Function of Amino Acids and Peptides Volume 5 of this series presents a wealth of methods to analyzeamino acids and peptides. Classical approaches are described, suchas X-ray analysis, chromatographic methods, NMR, AFM, massspectrometry and 2D-gel electrophoresis, as well as newerapproaches, including Surface Plasmon Resonance and arraytechnologies. Originally planned as a six volume series, Amino Acids,Peptides and Proteins in Organic Chemistry now completes withfive volumes but remains comprehensive in both scope andcoverage. ahref="http://eu.wiley.com/WileyCDA/WileyTitle/productCd-3527335463.html"Furtherinformation about the 5 Volume Set and purchasing details can beviewed here./a

Principles and Applications of Molecular Diagnostics

Author : Nader Rifai
Publisher : Elsevier
ISBN 13 : 0128160624
Total Pages : 424 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Principles and Applications of Molecular Diagnostics by : Nader Rifai

Download or read book Principles and Applications of Molecular Diagnostics written by Nader Rifai and published by Elsevier. This book was released on 2018-06-13 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. - Explains the principles and tools of molecular biology - Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results - Provides a detailed description of current molecular applications used to solve diagnostics tasks