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Affinity Chromatography And Molecular Interactions
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Book Synopsis Molecular Interactions in Bioseparations by : That T. Ngo
Download or read book Molecular Interactions in Bioseparations written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Book Synopsis Affinity Chromatography and Molecular Interactions by : Jean-Marc Egly
Download or read book Affinity Chromatography and Molecular Interactions written by Jean-Marc Egly and published by . This book was released on 1979 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Affinity Chromatography and Molecular Interactions by : Jean-Marc Egly
Download or read book Affinity Chromatography and Molecular Interactions written by Jean-Marc Egly and published by . This book was released on with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Affinity Chromatography by : Peter D. G. Dean
Download or read book Affinity Chromatography written by Peter D. G. Dean and published by Oxford University Press, USA. This book was released on 1985 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matrix preparation and applications; Activation procedures; Cross-linking agents for coupling matrices to spacers; Operational methodologies; Ligands for immobilisation; Quantitative characterisation of interactions by affinity chromatography; Zonal elution quantitative affinity chromatography and analysis of molecular interactions; Cell separation by affinity chromatography ligand immobilisation to solid supports through cleavable mercury-sulphur bonds.
Book Synopsis Affinity Chromatography by : Michael Zachariou
Download or read book Affinity Chromatography written by Michael Zachariou and published by Springer Science & Business Media. This book was released on 2008 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.
Book Synopsis Affinity Chromatography and Biological Recognition by : Irwin Chaiken
Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Book Synopsis Frontal Affinity Chromatography with Mass Spectrometry Detection for Probing Molecular Interactions. A New Mechanistic Approach for the Development of Detection & Identification and Medical Countermeasures Against CB Agents by :
Download or read book Frontal Affinity Chromatography with Mass Spectrometry Detection for Probing Molecular Interactions. A New Mechanistic Approach for the Development of Detection & Identification and Medical Countermeasures Against CB Agents written by and published by . This book was released on 2005 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt: Frontal affinity chromatography with mass spectrometry detection (FAC-MS) is a powerful technique for the study of molecular interactions between receptors (proteins, antibodies, enzymes) and ligands (small molecules, antigens, drug compounds). Such interactions are fundamental to biochemical pathways, from the detection of a protein cascade involved in a disease state, to the study of the disease mechanisms, to high-throughput screening for the identification and optimization of lead compounds for drug discovery. FAC-MS is able to locate and characterize key molecular recognitions in complex biological systems that are otherwise "hidden" from current technologies. Thus, this cutting-edge technique can steer biotechnological research through key areas and eliminate many years of "hit-and-miss" experimentation. It will allow for rational design and development in areas, such as bio-detection and identification, and medical countermeasure, which are currently of interest to the CF and DRDC. FAC-MS is amenable to high-throughput screening of large collections of compounds simultaneously. Currently, no other alternative method can provide unbiased, direct measures of binding affinity in a rapid and easy to perform manner as FAC-MS. This article outlines the plan of adapting FAC-MS from industry (Protana Inc.) to DRDC Suffield for defense research incorporating toxin research, drug discovery, recombinant antigen-antibody optimization and proteomics.
Book Synopsis Affinity Chromatography by : O. Hoffmann-Ostenhof
Download or read book Affinity Chromatography written by O. Hoffmann-Ostenhof and published by Elsevier. This book was released on 2016-01-22 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography is a collection of papers that presents the advancement in various areas of affinity chromatography. The title particularly covers the development in affinity chromatography in the context of biochemistry and immunochemistry. The text first details gel matrices, coupling methods, and charge transfer chromatography. Next, the selection tackles the affinity chromatography of enzymes and hydrophobic interaction chromatography. The text also discusses matrix-bound antigens and antibodies, along with matrix-bound lectins and affinity chromatography of cells. Part VI deals with affinity chromatography of hormone receptors, while part vii talks about affinity techniques in the isolation Of DNA, RNA, and polysomes. The book will be of great use to students, researchers, and practitioners of biochemistry.
Book Synopsis Affinity Chromatography as a Tool for Quantification of Interactions Between Drug Molecules and Their Protein Targets by : Piotr Draczkowski
Download or read book Affinity Chromatography as a Tool for Quantification of Interactions Between Drug Molecules and Their Protein Targets written by Piotr Draczkowski and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography as a Tool for Quantification of Interactions Between Drug Molecules and Their Protein Targets.
Book Synopsis Chromatographic Determination of Molecular Interactions Applications in Biochemistry, Chemistry, and Biophysics by : Tibor Cserhati
Download or read book Chromatographic Determination of Molecular Interactions Applications in Biochemistry, Chemistry, and Biophysics written by Tibor Cserhati and published by CRC Press. This book was released on 1993-10-13 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatographic Determination of Molecular Interactions describes the theory and practice of the measurement of molecular interactions by thin-layer, high performance liquid, and gas chromatography. Methods and various procedures used for the calculation of complex stability constants are compiled, and the stability constants of a wide variety of interactions determined by the various chromatographic techniques are included. New results of molecular interactions are covered, including those for protein-peptide and amino acid-nucleic acid bases. The book will appeal to biochemists, analytical chemists, molecular biologists, biotechnologists, biophysicists, and medicinal chemists.
Book Synopsis Affinity Chromatography by : P. Mohr
Download or read book Affinity Chromatography written by P. Mohr and published by CRC Press. This book was released on 1985-11-22 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography combines theoretical aspects and practical applications-providing a solid understanding of affinity principles on a molecular level. Beginning with the historical background of affinity chromatography, this single-source volume discusses matrix supports and the insertion of spacers ... the chemical and physicochemical features of the adsorption and elution step ... immunoassay technology and separation of viruses and cells ... variants of affinity chromatography other than biospecific ... related techniques including affinity electrophoresis ... and much more. Written by leading experts in the field, Affinity Chromatography contains convenient features such as a concise outline format-summarizing useful information; numerous illustrations-clarifying fundamental and methodical factors; easy-to-read tables-for a quick understanding of vital material; current references-facilitating continuing study. Providing stimulation for the development of new affinity methods, this important volume is mandatory for analytical chemists, chromatographers, biochemists, biologists, microbiologists, pharmacists, and students in advanced undergraduate- and graduate-level analytical and preparative chemistry courses. Book jacket.
Book Synopsis The Study of Small Molecule-ligand Interactions in High Performance Affinity Chromatography Using Immobilized Triazine Dyes by : Tina Marie Christine Wade
Download or read book The Study of Small Molecule-ligand Interactions in High Performance Affinity Chromatography Using Immobilized Triazine Dyes written by Tina Marie Christine Wade and published by . This book was released on 1988 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analytical Affinity Chromatography by : Irwin M. Chaiken
Download or read book Analytical Affinity Chromatography written by Irwin M. Chaiken and published by CRC Press. This book was released on 2018-01-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).
Book Synopsis Affinity Chromatography by : Pascal Bailon
Download or read book Affinity Chromatography written by Pascal Bailon and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Well-versed practitioners detail powerful affinity chromatography methods, ranging from such traditional affinity purification as immunoaffinity chromatography, to the use of state-of-the-art phage-display technology in the discovery of affinity ligands and drugs. Also included are effective and reproducible separations of such small molecules as haptens, protein ligands, and supramolecular structures. Each chapter is devoted to a specific technique and includes an introduction, an explanation of principles, a detailed materials list, and step-by-step instructions that ensure experimental success. Practical notes suggest alternative procedures and describe how to overcome problems that may occur. State-of-the-art and eminently instructive, Affinity Chromatography: Methods and Protocols offers novice and experienced scientists alike a comprehensive collection of proven and readily reproducible techniques that are ideally suited for use not only in drug discovery and development, but also in work on affinity interactions, fermentations, separations, drug targeting, and cell culture.
Book Synopsis Affinity Chromatography by : Sameh Magdeldin
Download or read book Affinity Chromatography written by Sameh Magdeldin and published by BoD – Books on Demand. This book was released on 2012-03-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers.
Book Synopsis Quantitative In Silico Chromatography by : Toshihiko Hanai
Download or read book Quantitative In Silico Chromatography written by Toshihiko Hanai and published by Royal Society of Chemistry. This book was released on 2014-06-15 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the author of "HPLC: A Practical Guide" (RSC, 1999), this book presents the possibilities for characterising biological applications by combining analytical and computational chemistries.
Book Synopsis Handbook of Affinity Chromatography by : Toni Kline
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
