Advancing Tandem Mass Spectrometry for Characterization of Complex Biopolymers

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ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (143 download)

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Book Synopsis Advancing Tandem Mass Spectrometry for Characterization of Complex Biopolymers by : Trenton M. Peters-Clarke

Download or read book Advancing Tandem Mass Spectrometry for Characterization of Complex Biopolymers written by Trenton M. Peters-Clarke and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation details innovations in mass spectrometry-based instrumentation that expand our ability to survey biopolymers, including proteins, peptides, and oligonucleotides, with increased sensitivity, speed, and depth. Chapter 1 provides an overview of mass spectrometry instrumentation at the leading edge of proteomics and for nucleic acid analysis. In Chapter 2, the optical-fiber enabled coupling of a mass spectrometer to an infrared laser improves proteomic characterization. For Chapter 3, electron transfer dissociation-based proteomics is investigated and inefficiencies of related ion-ion reactions are characterized. Chapter 4 introduces a novel nucleic acid sequencing technology with activated ion-negative electron transfer dissociation to profile RNA molecules and their modifications. In Chapter 5, tandem mass spectrometry comprehensively characterizes heavily-modified RNA therapeutics. Chapter 6 focuses on a proteome-wide analysis of the bacterial post-translational modification O-mycoloylation. In Chapter 7, a novel tandem mass spectrometry strategy, termed activated ion-tandem mass tags, is revealed, boosting quantitative reporter ion generation. Chapter 8 expands upon AI-TMT for quantitative single-cell proteomics, realizing sensitivity gains which drive a greater dynamic range of quantification. In Chapter 9, a novel mass spectrometer enables the one hour human proteome and we explore one minute proteome analyses, pushing the limits of high-throughput proteome analysis. Chapter 10 discusses emergent proteomic techniques and suggests future directions of the field.

Mass Spectrometry Techniques for the Characterization of Synthetic Polymers, Biopolymers, Biodegradation Products and Their Interactions

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ISBN 13 :
Total Pages : 178 pages
Book Rating : 4.:/5 (91 download)

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Book Synopsis Mass Spectrometry Techniques for the Characterization of Synthetic Polymers, Biopolymers, Biodegradation Products and Their Interactions by : Madalis Casiano-Maldonado

Download or read book Mass Spectrometry Techniques for the Characterization of Synthetic Polymers, Biopolymers, Biodegradation Products and Their Interactions written by Madalis Casiano-Maldonado and published by . This book was released on 2012 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: The characterization of synthetic polymers and complex oligomeric mixtures by a combination of different mass spectrometry (MS) techniques is the main topic of this dissertation. Ion mobility (IM) separation and/or tandem mass spectrometry (MS2) were interfaced with single stage mass spectrometry for the fast, complete, precise and accurate characterization of different polymers, biopolymers, and biodegradation products. Additionally, the development of a mass spectrometry protocol for the quantitation of proteins adsorbed on different polymer surfaces was accomplished in this dissertation. Over the passed years, synthetic routes for the creation of PEGs have been modified by the elimination of organic catalysts and solvents and the application of green chemistry for the generation of new polymers. In Chapter 4 of this dissertation, MS and MS2 were employed, as the main analytical techniques, for the structural elucidation of enzyme-catalyzed, functionalized PEG and tetraethylene glycol (TEG) biomaterials. The samples analyzed were synthesized by two different processes, transesterification and Michael addition reactions, both of them using CALB as the catalyst. In Chapter 5 of this dissertation, polylactide was degraded using Proteinase K and its biodegradation products were analyzed by MS and MS2. Elucidation of its degradation products is important to the biomedical community; knowledge of these products helps to see any toxicity problems with the use of this polymer. The MS and MS2 results showed that the degradation products are short polylactide chains, down to the monomer. No other, potentially dangerous organics were detected. In the following chapter of this dissertation (Chapter 6), the adsorption of three model proteins onto two different surfaces was evaluated over a pH range. The results were explained on terms of polymer chemistry, surface morphology, proteins' isoelectricpoints and molecular dimensions. Finally, Chapter 7 reports how derivatization, degradation, or chromatographic separation can be avoided for the analysis of complex systems, if multidimensional mass spectrometry methods combining ion mobility separation and different ionization techniques are employed. Using this new approach complex poly([alpha]-peptoid) samples were characterized. The results showed that the main product in the system contained the N-heterocyclic carbene (NHC) moiety that served as ring-opening polymerization catalyst. Furthermore, it was demonstrated that elimination of the NHC segment leads to cyclic poly([alpha]-peptoid)s that have very similar conformations with comparably sized polypeptides.

Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry

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Publisher : John Wiley & Sons
ISBN 13 : 1119814057
Total Pages : 565 pages
Book Rating : 4.1/5 (198 download)

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Book Synopsis Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry by : Haroun N. Shah

Download or read book Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2023-06-06 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry Detailed resource presenting the capabilities of MALDI mass spectrometry (MS) to industrially and environmentally significant areas in the biosciences Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry fulfills a need to bring the key analytical technique of MALDI mass spectrometric analysis into routine practice by specialists and non-specialists, and technicians. It informs and educates established researchers on the development of techniques as applied to industrially significant areas within the biosciences. Throughout the text, the reader is presented with recognized and emerging techniques of this powerful and continually advancing field of analytical science to key areas of importance. While many scientific papers are reporting new applications of MS-based analysis in specific foci, this book is unique in that it draws together an incredibly diverse range of applications that are pushing the boundaries of MS across the broad field of biosciences. Contributed to by recognized experts in the field of MALDI MS who have been key players in promoting the advancement and dissemination of authoritative information in this field, Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry covers sample topics such as: Oil microbiology, marine and freshwater ecosystems, agricultural and food microbiology, and industrial waste microbiology Bioremediation and landfill sites microbiology, microbiology of inhospitable sites (e.g. Arctic and Antarctic, and alkaline and acidic sites, and hot temperatures) Veterinary, poultry and animals, viral applications of MS, and antibiotic resistance using tandem MS methods Recent developments which are set to transform the use of MS from its success in clinical microbiology to a wide range of commercial and environmental uses Bridging the gap between measurement and key applications, this text is an ideal resource for industrial and environmental analytical scientists, including technologists in the food industry, pharmaceuticals, and agriculture, as well as biomedical scientists, researchers, clinicians and academics and scientists in bio-resource centers.

Tandem Fourier-transform Mass Spectrometric Methods for the Characterization of Biopolymers and Noncovalent Complexes

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ISBN 13 :
Total Pages : 728 pages
Book Rating : 4.:/5 (34 download)

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Book Synopsis Tandem Fourier-transform Mass Spectrometric Methods for the Characterization of Biopolymers and Noncovalent Complexes by : Paul David Schnier

Download or read book Tandem Fourier-transform Mass Spectrometric Methods for the Characterization of Biopolymers and Noncovalent Complexes written by Paul David Schnier and published by . This book was released on 1998 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tandem Mass Spectrometry

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Publisher : BoD – Books on Demand
ISBN 13 : 9535111361
Total Pages : 208 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Tandem Mass Spectrometry by : Ana Varela Coelho

Download or read book Tandem Mass Spectrometry written by Ana Varela Coelho and published by BoD – Books on Demand. This book was released on 2013-05-29 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tandem Mass Spectrometry - Molecular Characterization presents a comprehensive coverage of theory, instrumentation and description of experimental strategies and MS/MS data interpretation for the structural characterization of relevant molecular compounds. The areas covered include the analysis of drugs, metabolites, carbohydrates and protein post-translational modifications. The book series in Tandem Mass Spectrometry serves multiple groups of audiences; professional (academic and industry), graduate students and general readers interested in the use of modern mass spectrometry in solving critical questions of chemical and biological sciences.

Mass Spectrometry of Biological Materials, Second Edition

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Publisher : CRC Press
ISBN 13 : 9780824701574
Total Pages : 490 pages
Book Rating : 4.7/5 (15 download)

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Book Synopsis Mass Spectrometry of Biological Materials, Second Edition by : Barbara S. Larsen

Download or read book Mass Spectrometry of Biological Materials, Second Edition written by Barbara S. Larsen and published by CRC Press. This book was released on 1998-03-02 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Second Edition provides up-to-the-minute discussions on the application of mass spectrometry to the biological sciences. Shows how and why experiments are performed and furnishes details to facilitate duplication of results.

Advancing Bottom-up Proteomics and Ptm Characterization Via Development of Novel Mass Spectrometry-based Approaches

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ISBN 13 :
Total Pages : 335 pages
Book Rating : 4.:/5 (131 download)

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Book Synopsis Advancing Bottom-up Proteomics and Ptm Characterization Via Development of Novel Mass Spectrometry-based Approaches by : Fengfei Ma (Ph.D.)

Download or read book Advancing Bottom-up Proteomics and Ptm Characterization Via Development of Novel Mass Spectrometry-based Approaches written by Fengfei Ma (Ph.D.) and published by . This book was released on 2018 with total page 335 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS) has emerged as a central technology for biomedical research largely due to its capability to provide unprecedented insights into the compositions and structures of the proteome. Thousands of proteins and peptides can be characterized in a single experiment, shedding lights on the complex biological processes and enabling discovery of novel therapeutic targets. This dissertation is devoted to the development of novel liquid chromatography (LC)-MS based techniques to characterize proteins and decipher post-translational modifications (PTMs) qualitatively and quantitatively. Specifically, novel sample preparation strategy has been developed for improved sample recovery and unbiased extraction of different categories of proteins. State-of-the-art hybrid fragmentation methods have been established for large scale differentiation of 3- and 4- hydroxyproline isomers. Furthermore, an integrated pipeline for in-depth investigation of protein glycosylation has been developed and implemented. These innovative analytical methodologies have been successfully demonstrated and applied to comprehensive characterization of biological scaffolds and breast cancer cells, which provide valuable insights into multiple research areas including tissue engineering and cancer biology.

Characterization of Impurities and Degradants Using Mass Spectrometry

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Publisher : Wiley
ISBN 13 : 9780470386187
Total Pages : 0 pages
Book Rating : 4.3/5 (861 download)

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Book Synopsis Characterization of Impurities and Degradants Using Mass Spectrometry by : Guodong Chen

Download or read book Characterization of Impurities and Degradants Using Mass Spectrometry written by Guodong Chen and published by Wiley. This book was released on 2011-05-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book highlights the current practices and future trends in structural characterization of impurities and degradants. It begins with an overview of mass spectrometry techniques as related to the analysis of impurities and degradants, followed by studies involving characterization of process related impurities (including potential genotoxic impurities), and excipient related impurities in formulated products. Both general practitioners in pharmaceutical research and specialists in analytical chemistry field will benefit from this book that will detail step-by-step approaches and new strategies to solve challenging problems related to pharmaceutical research.

Mass Spectrometry in the Biological Sciences

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Publisher : Springer Science & Business Media
ISBN 13 : 1461202299
Total Pages : 569 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Mass Spectrometry in the Biological Sciences by : A. L. Burlingame

Download or read book Mass Spectrometry in the Biological Sciences written by A. L. Burlingame and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin. They demonstrate by example how these methodologies can solve a wide array of real-world problems in protein biochemistry, immunology, and glycobiology, as well as for human bacterial pathogens, lipids, and nucleic acids. The book offers a unique opportunity to learn these techniques that are revolutionizing the field. Its authoritative assessment in the context of how to solve important and challenging problems in bioscience and medicine ensures a competitive advantage for today's researchers.

Selective Structural Characterization of Complex Polymers by Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry

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ISBN 13 :
Total Pages : 218 pages
Book Rating : 4.:/5 (484 download)

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Book Synopsis Selective Structural Characterization of Complex Polymers by Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry by : Haining Ji

Download or read book Selective Structural Characterization of Complex Polymers by Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry written by Haining Ji and published by . This book was released on 2001 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tandem Mass Spectrometry Approaches to Characterizing Challenging Biomolecules

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ISBN 13 :
Total Pages : 140 pages
Book Rating : 4.:/5 (966 download)

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Book Synopsis Tandem Mass Spectrometry Approaches to Characterizing Challenging Biomolecules by : Christopher Martin Crittenden

Download or read book Tandem Mass Spectrometry Approaches to Characterizing Challenging Biomolecules written by Christopher Martin Crittenden and published by . This book was released on 2016 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry has emerged as a leading tool in the field of chemistry as an analytical method for the characterization of small molecules, proteins, and other complex biomolecules. Specifically, cyclic and stapled peptides have become an intriguing class of biomolecules in drug research afforded to them because of their biological stability and resistance to proteolytic digestions. However, challenges are presented in regards to the characterization of these molecules as traditional methods are ineffective in determining a ring-opening site on the peptidic backbone. Additionally, lipid A, the hydrophobic domain of lipopolysaccharide (LPS), consists of a diglucosamine backbone and is responsible for fastening LPS to a membrane surface. Lipid A becomes a biologically relevant molecule to study as its function within LPS is directly related to the infectious and toxic properties of gram-negative bacteria, but the molecule is structurally complex and offers many challenges in terms of traditional mass spectrometry characterization. Presented in the thesis are methods to further comprehend structural motifs related to the aforementioned biomolecules. The "ornithine effect", which describes the conversion of an arginine residue to an ornithine residue via reaction with hydrazine and subsequent preferential cyclization via nucleophilic attack of the ornithine side-chain to the neighboring carbonyl group, inducing heterolytic cleavage of the adjacent amide bond under gentle activation, is used to preferentially open cyclic and stapled rings to linearize these challenging biomolecules. Ramped collisional and photon based activation (in terms of energy and laser pulses) of lipid A molecules that contain differences in acyl-chain length and connectivity reveal general trends about the lability of certain bonds on the lipid A molecules themselves and paints a picture of the overall fragmentation trends associated with variations in lipid A structural motifs.

New Methods for the Study of Biomolecular Complexes

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Publisher : Springer
ISBN 13 : 9789048150175
Total Pages : 354 pages
Book Rating : 4.1/5 (51 download)

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Book Synopsis New Methods for the Study of Biomolecular Complexes by : W. Ens

Download or read book New Methods for the Study of Biomolecular Complexes written by W. Ens and published by Springer. This book was released on 2010-12-15 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: A NATO Advanced Research Workshop entitled New Methods for the Study of Molecular Aggregates was held at Tbe Lodge at Kananaskis Village, Alberta, Canada from 16 -20 June 1996. In fact the meeting was entirely concerned with the problem of analyzing biomolecular complexes, so the title of these proceedings has been altered to give a more precise description of the content. Tbe workshop was hosted by the time-of-flight group of the Department of Physics at the University of Manitoba, and was attended by 64 participants from around the world. '!\venty-one invited talks were given and 27 papers were presented as posters. Of the 48 contributions, 22 papers (12 orals, 10 posters) are included in these proceedings. Tbe subject of the conference was the investigation of noncovalent biomolecular complexes, with particular focus on the application of mass spectrometry to their characterization. '!\vo new ionization techniques introduced in the late 1980s, electrospray ionization (ES I) and matrix-assisted laser desorptionlionization (MALDI), resulted in a breakthrough in mass spectrometry, enabling its use in molecular weight and primary structure determination of biopolymers larger than 100 kDa. Recently it has been discovered that ESI mass spectrometry mayaiso be used to characterize complexes containing noncovalent interactions, thus opening new perspectives for supramolecular chemistry. ESI mass spectrometry has the advantage that the sampie is introduced from a homogenous solution which can be maintained at near physiological conditions of pR, concentration, and temperature.

Structural Characterization of Proteins and Anionic Biopolymers by Desorption-ionization Mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 516 pages
Book Rating : 4.:/5 (279 download)

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Book Synopsis Structural Characterization of Proteins and Anionic Biopolymers by Desorption-ionization Mass Spectrometry by : Rong Wang

Download or read book Structural Characterization of Proteins and Anionic Biopolymers by Desorption-ionization Mass Spectrometry written by Rong Wang and published by . This book was released on 1992 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Structural Characterization of Synthetic Polymers and Copolymers Using Multidimensional Mass Spectrometry Interfaced with Thermal Degradation, Liquid Chromatography And/or Ion Mobility Separation

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Publisher :
ISBN 13 :
Total Pages : 238 pages
Book Rating : 4.:/5 (911 download)

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Book Synopsis Structural Characterization of Synthetic Polymers and Copolymers Using Multidimensional Mass Spectrometry Interfaced with Thermal Degradation, Liquid Chromatography And/or Ion Mobility Separation by : Nadrah Alawani

Download or read book Structural Characterization of Synthetic Polymers and Copolymers Using Multidimensional Mass Spectrometry Interfaced with Thermal Degradation, Liquid Chromatography And/or Ion Mobility Separation written by Nadrah Alawani and published by . This book was released on 2013 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation focuses on coupling mass spectrometry (MS) and tandem mass spectrometry (MS/MS) to thermal degradation, liquid chromatography (LC) and/or ion mobility (IM) spectrometry for the characterization of complex mixtures. In chapter II, an introduction of the history and the principles of mass spectrometry (MS) and liquid chromatography (LC) are discussed. Chapter III illustrates the materials and instrumentation used to complete this dissertation. Polyethers have been characterized utilizing tandem mass spectrometry (MS/MS), as presented in Chapter IV and Chapter VI. Diblock copolymers of polyethylene oxide and polycaprolactone, PEO-b-PCL, have been characterized by matrix-assisted laser desorption/ionization quadrupole/time-of-flight mass spectrometry (MALDI-Q/ToF) and LC-MS/MS (Chapter V). Thermoplastic elastomers have been characterized by thermal degradation using an atmospheric solids analysis probe (ASAP) and ion mobility mass spectrometry (IM-MS), as discussed in Chapter VII. Interfacing separation techniques with mass spectrometry permitted the detection of species present with low concentration in complex materials and improved the sensitivity of MS. In chapter IV, the fragmentation mechanisms in MS/MS experiments of cyclic and linear poly(ethylene oxide) macroinitiators are discussed. This study aimed at determining the influence of end groups on the fragmentation pathways. In the study reported in Chapter V, ultra high performance liquid chromatography (UHPLC) was interfaced with MS and MS/MS to achieve the separation and in-depth characterization and separation of amphiphilic diblock copolymers (PEO-b-PCL) in which the architecture of the PEO block is linear or cyclic. Applying UPLC-MS and UPLC-MS/MS provides fast accurate information about the number and type of the blocks in the copolymers. Chapter VI reports MS/MS and IM-MS analyses which were performed to elucidate the influence of molecular size and collision energy on the fragmentation pathways of polyethers subjected to collisionally activated dissociation (CAD). Survival yields and collision cross-sections were derived for several oligomers of polybutylene oxide (PBO) and polytetrahydrofuran (PTHF) polymers by MS/MS and IM-MS, in order to understand their fragmentation energetics and fully characterize their structures. In Chapter VII, application of atmospheric solids analysis probe (ASAP) and ion mobility (IM) separation were coupled with mass spectrometry (MS) and tandem mass spectrometry (MS/MS) to characterize commercially available thermoplastic elastomers. These compounds are mainly composed of thermoplastic copolymers, but also contain additional chemicals to enhance their properties or to protect them from degradation. Using ASAP-IM-MS enables fast analysis, involving mild degradation at atmospheric pressure (ASAP) and subsequent characterization of the desorbates and pyrolyzates by ion mobility mass spectrometry (IM-MS) and tandem mass spectrometry (MS/MS). Such multidimensional dispersion considerably simplifies the resulting spectra, permitting the conclusive separation and characterization of the multicomponent materials examined. Chapter VIII summarizes the findings of this dissertation and is followed by appendices with supplemental data and the copyright permissions obtained for this dissertation.

Structural Characterization of Complex Polymer Systems by Degradation/mass Spectrometry

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Publisher :
ISBN 13 :
Total Pages : 204 pages
Book Rating : 4.:/5 (226 download)

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Book Synopsis Structural Characterization of Complex Polymer Systems by Degradation/mass Spectrometry by : Panthida Thomya

Download or read book Structural Characterization of Complex Polymer Systems by Degradation/mass Spectrometry written by Panthida Thomya and published by . This book was released on 2006 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The compositions and structures of large and complex macromolecular systems have been analyzed by combining low-temperature pyrolysis (

Tandem Mass Spectrometry and Ion-molecule Interactions

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Publisher :
ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.:/5 (398 download)

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Book Synopsis Tandem Mass Spectrometry and Ion-molecule Interactions by : Asoka Ranasinghe

Download or read book Tandem Mass Spectrometry and Ion-molecule Interactions written by Asoka Ranasinghe and published by . This book was released on 1992 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Tandem Mass Spectrometry

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ISBN 13 :
Total Pages : 207 pages
Book Rating : 4.:/5 (145 download)

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Book Synopsis Tandem Mass Spectrometry by : Kathleen Elizabeth Singleton

Download or read book Tandem Mass Spectrometry written by Kathleen Elizabeth Singleton and published by . This book was released on 1985 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: