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Book Synopsis Advancing Cryptosporidium Parvum Detection Methodologies by : Mark C. Jenkins
Download or read book Advancing Cryptosporidium Parvum Detection Methodologies written by Mark C. Jenkins and published by American Water Works Association. This book was released on 2002 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.
Download or read book Cryptosporidium written by Mark S Smith and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryptosporidium, in its various forms, is a widely recognised cause of outbreaks of waterborne disease. Regulatory bodies worldwide are increasingly requiring the development of "fit-for-purpose" detection methods for this protozoan parasite, but analysis is often problematic. Bringing together international academic and industry-based experts, this book provides a comprehensive review of the current state of analytical techniques for the detection of Cryptosporidium, as well as looking at likely future developments. In particular, the issues of species identification and oocyst viability are addressed. Quality assurance issues and potential problems associated with the new Cryptosporidium regulations are also highlighted. The extent of the perceived problems and the regulatory backdrop against which the analysis must be carried out are also discussed. Scientists in the water industry, environmental testing laboratories, researchers, consultants, environmental health professionals, food manufacturers and regulatory or environmental bodies are amongst the many who should read this book. In addition, anyone with an interest in microbiological challenges and problem-solving will welcome the coverage.
Book Synopsis Evaluating Methodologies for Detection and Enumeration of Enteric Viruses and Cryptosporidium Parvum by : Laura Yvette Sifuentes
Download or read book Evaluating Methodologies for Detection and Enumeration of Enteric Viruses and Cryptosporidium Parvum written by Laura Yvette Sifuentes and published by . This book was released on 2007 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :University of Guelph. Department of Environmental Biology Publisher : ISBN 13 :9780494110935 Total Pages :212 pages Book Rating :4.1/5 (19 download)
Book Synopsis Molecular-based Methods for the Detection and Quantification of Infectious Cryptosporidium Parvum Oocysts in Environmental Samples by : University of Guelph. Department of Environmental Biology
Download or read book Molecular-based Methods for the Detection and Quantification of Infectious Cryptosporidium Parvum Oocysts in Environmental Samples written by University of Guelph. Department of Environmental Biology and published by . This book was released on 2005 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cryptosporidium Parvum Oocysts by : Satomi Kato
Download or read book Cryptosporidium Parvum Oocysts written by Satomi Kato and published by . This book was released on 2001 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development of a PCR Based Method to Detect Viable Cryptosporidium Parvum Oocysts from Environmental Water Concentrates by : Debanjana Dasgupta
Download or read book Development of a PCR Based Method to Detect Viable Cryptosporidium Parvum Oocysts from Environmental Water Concentrates written by Debanjana Dasgupta and published by . This book was released on 2003 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cryptosporidium and Cryptosporidiosis by : Ronald Fayer
Download or read book Cryptosporidium and Cryptosporidiosis written by Ronald Fayer and published by CRC Press. This book was released on 2007-12-25 with total page 905 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the microscopic observation of infection to the widespread application of molecular techniques in taxonomy and epidemiology, to the genome sequencing of two major species and advances in biochemistry, phylogeny, and water treatment, new information on this fascinating genus continues to mount as we discover and utilize the latest scientific te
Book Synopsis Evaluation of TaqMan Real-time PCR for the Detection of Viable Cryptosporidium Parvum Oocysts in Environmental Water Samples by : Melissa A. Cameron
Download or read book Evaluation of TaqMan Real-time PCR for the Detection of Viable Cryptosporidium Parvum Oocysts in Environmental Water Samples written by Melissa A. Cameron and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Cryptosporidium parvum is of growing public health concern due to its ability to survive typical water treatment processes. In order to protect the public from infection, the Environmental Protection Agency developed Method 1623 for the detection of Cryptosporidium oocysts in environmental water samples. Execution of this method is time consuming, and the results do not provide an accurate estimation of viability. Therefore, current research is focused on creating a real-time PCR method for the accurate detection of viable Cryptosporidium parvum in environmental water samples. This thesis presents the development of a real-time PCR method, and the results obtained in its use on field samples. The assay was standardized using multiple dilution series in addition to positive and negative controls. Environmental water samples were tested using this method and Method 1623 for comparison. The results were compared statistically to determine the degree of correlation between methods. The data show that the real-time PCR method correlates well to Method 1623. In addition, the assay was determined to be more cost effective and less labor intensive than Method 1623. Although these early findings are promising, additional research and development are needed before the proposed assay can be used in industry.
Book Synopsis Detection of "Cryptosporidium Parvum" Oocysts in Faeces by : Katherine A. Webster
Download or read book Detection of "Cryptosporidium Parvum" Oocysts in Faeces written by Katherine A. Webster and published by . This book was released on 1996 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Improved in Vitro Culture of Cryptosporidium parvum by : G. Widmer
Download or read book Improved in Vitro Culture of Cryptosporidium parvum written by G. Widmer and published by IWA Publishing. This book was released on 2006-04-30 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this project was to investigate methods, which promote growth of Cryptosporidium (C.) parvum in culture. The researchers tested the assumption that improved survival of host cells enhances growth of C. parvum. Although certain modifications to the culture methodology improved host cell survival, enhancing host cell survival was not always accompanied by an increase in parasite density in the cell monolayer. Some cultures grown on collagen or laminin showed improved parasite growth. However, this effect was not consistent, indicating that other variables affect parasite growth. C. parvum was more prevalent in cells in the mitotic cycle than in non-dividing cells. This is consistent with the absence of certain biosynthetic pathways in C. parvum, and suggests that dividing cells may be more favorable to parasite development. Although current culture methods enable the detection of infectious Cryptosporidium oocysts, limited parasite growth reduces the sensitivity of the assay, making it necessary to apply sophisticated methods for the detection of the intracellular parasites. This study identified methods that slightly improve growth of Cryptosporidium in culture and also highlighted methods that have no effect. This report is available only as a pay-per-view item.
Book Synopsis Development of a New Method to Detect Cryptosporidium Parvum Oocysts from Water Samples by Using Aluminium Sulphate Flocculation and Immunomagnetic Separation by : Christoph Bissegger
Download or read book Development of a New Method to Detect Cryptosporidium Parvum Oocysts from Water Samples by Using Aluminium Sulphate Flocculation and Immunomagnetic Separation written by Christoph Bissegger and published by . This book was released on 1997 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cryptosporidiosis and Microsporidiosis by : Franz Petry
Download or read book Cryptosporidiosis and Microsporidiosis written by Franz Petry and published by Karger Medical and Scientific Publishers. This book was released on 2000-01-01 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: '... This volume provides most complete and balanced coverage of essential aspects of the pathogens as well as the diagnosis and clinical correlations of the disease they cause. It is set to become a valuable refrence for parasitologists, protozoologists, molecular biologists, clinical mecrobiologists, epidemiologists and specialists in infectious diseases.'
Book Synopsis Detection of Selected Enzyme Activities in Cryptosporidium Parvum by : Shamsuzzaman Malek
Download or read book Detection of Selected Enzyme Activities in Cryptosporidium Parvum written by Shamsuzzaman Malek and published by . This book was released on 1996 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development of a Strain Specific Diagnostic/detection Assay for Cryptosporidium Parvum by : Cindy Louise Gibbons
Download or read book Development of a Strain Specific Diagnostic/detection Assay for Cryptosporidium Parvum written by Cindy Louise Gibbons and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Detection of Cryptosporidium and E. Coli Using Fluorescent in Situ Hybridization and Solid Phase Laser Cytometry by : Susan Cameron Broadaway
Download or read book Detection of Cryptosporidium and E. Coli Using Fluorescent in Situ Hybridization and Solid Phase Laser Cytometry written by Susan Cameron Broadaway and published by . This book was released on 2013 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryptosporidium parvum is a protozoal pathogen transmitted through water by the fecal-oral route as oocysts. Because the oocysts are more resistant to environmental stresses than the bacteria conventionally used as indicators of fecal contamination, they can be present in water when indicator organisms, such as E. coli, are not found. In addition, because they are resistant to chlorine, they can pass from source water through water treatment into drinking water systems. The EPA method for detection of Cryptosporidium oocysts consists of identifying oocysts with fluorescently labeled antibodies, staining with 4',6-diamidino-2-phenylindole and examining slides with epifluorescent microscopy and differential interference contrast microscopy. This protocol is labor intensive and subject to technician error. A new method was developed for the rapid detection of Cryptosporidium parvum oocysts using fluorescent in situ hybridization (FISH) and the ScanRDI, a solid phase laser cytometer. Optimization of the FISH protocol for use with the ScanRDI was done with E. coli cells and known Cryptosporidium oocysts as a model. Source water and treated drinking water from the water treatment plant at Crow Agency on the Crow Indian Reservation in Montana was collected over the course of a year and concentrated using the EPA protocol for collection of oocysts. The samples were then examined for Cryptosporidium oocysts using both the ScanRDI method and the standard US EPA method. The combination of FISH for labeling Cryptosporidium and the ScanRDI for examination results in significantly higher numbers of Cryptosporidium detected as well as greater ease in identification. A statistical comparison was done that determined there was no correlation between the number of E. coli cells found in the water samples and the number of Cryptosporidium oocysts present. Additionally, although not tested on environmental samples, the FISH/ScanRDI method allowed for simultaneous detection of Cryptosporidium parvum oocysts and E. coli cells on the same membrane filter. Membranes were incubated before hybridization, hybridized concurrently with a Cryptosporidium specific probe and a probe specific for E. coli, followed by detection for both organisms with the ScanRDI.
Book Synopsis Giardia and Cryptosporidium by : Guadalupe Ortega-Pierres
Download or read book Giardia and Cryptosporidium written by Guadalupe Ortega-Pierres and published by CABI. This book was released on 2009 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: Giardia and Cryptosporidium are both parasites of considerable global interest due to the gastrointestinal problems these organisms can cause in humans as well as domestic and wild animals. Despite its long history as the causative agent of giardiasis, the clinical and zoonotic significance of Giardia infections in humans and animals is only beginning to emerge with the application of molecular tools. Less is known about Cryptosporidium and studies seek to understand the impact of infection in livestock and other animals, its epidemiology and zoonotic significance. Presenting a comprehensive overview of recent research this book draws on the experience of experts in all fields of Giardia and Cryptosporidium research.
Book Synopsis Technological Innovations in Sensing and Detection of Chemical, Biological, Radiological, Nuclear Threats and Ecological Terrorism by : Ashok Vaseashta
Download or read book Technological Innovations in Sensing and Detection of Chemical, Biological, Radiological, Nuclear Threats and Ecological Terrorism written by Ashok Vaseashta and published by Springer Science & Business Media. This book was released on 2012-01-03 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book arises from the NATO Advanced Study Institute “Technological Innovations in Detection and Sensing of CBRN Agents and Ecological Terrorism” held in Chisinau, Republic of Moldova in June 2010. It comprises a variety of invited contributions by highly experienced educators, scientists, and industrialists, and is structured to cover important aspects of the field that include developments in chemical-biological, and radiation sensing, synthesis and processing of sensors, and applications of sensors in detecting/monitoring contaminants introduced/dispersed inadvertently or intentionally in air, water, and food supplies. The book emphasizes nanomaterials and nanotechnology based sensing and also includes a section on sensing and detection technologies that can be applied to information security. Finally, it examines regional, national, and international policies and ethics related to nanomaterials and sensing. It will be of considerable interest and value to those already pursuing or considering careers in the field of nanostructured materials and nanotechnology based sensing, In general, it serves as a valuable source of information for those interested in how nanomaterials and nanotechnologies are advancing the field of sensing, detection, and remediation, policy makers, and commanders in the field.
