A Manual For Primary Human Cell Culture

Download A Manual For Primary Human Cell Culture full books in PDF, epub, and Kindle. Read online A Manual For Primary Human Cell Culture ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!

A Manual for Primary Human Cell Culture

Author : Jan-Thorsten Schantz
Publisher : World Scientific
ISBN 13 : 9789812387967
Total Pages : 176 pages
Book Rating : 4.3/5 (879 download)

DOWNLOAD NOW!

Book Synopsis A Manual for Primary Human Cell Culture by : Jan-Thorsten Schantz

Download or read book A Manual for Primary Human Cell Culture written by Jan-Thorsten Schantz and published by World Scientific. This book was released on 2004 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: As part of the boom in biotechnology, particularly in tissue engineering, primary human cell culture has become a major pillar in academic research and the biopharmaceutical industry. Obtaining a viable culture from a tissue sample and maintaining it for experimental, diagnostic or therapeutic purposes can be quite a challenge. Based on laboratory protocols and practical experience from many years of primary cell culture, this manual presents the basic steps necessary for culturing primary human cells. Written by students for students, the manual serves well as a practical guide to primary human cell culture. The authors have left much space for notes and the design of the manual is such that it can be continuously upgraded and extended. The content of this manual is by no means exhaustive. Protocols for specific cell types, out of over 200 different cell types in the human body, were selected from major tissue groupings in the body. They should serve as a foundation for individual researchersto experiment, explore, and establish niche protocols for their specific needs. Inspired by the practical clinical checklists available to residents and trainees in medicine, the authors have chosen a compact physical format that can fit into the pocket of a lab coat.

Culture of Animal Cells

Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 0470649356
Total Pages : 766 pages
Book Rating : 4.4/5 (76 download)

DOWNLOAD NOW!

Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2011-02-02 with total page 766 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture

Culture of Animal Cells

Author : R. Ian Freshney
Publisher :
ISBN 13 :
Total Pages : 420 pages
Book Rating : 4.:/5 (319 download)

DOWNLOAD NOW!

Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by . This book was released on 1987 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cultured!cell - Biohazards - Sterilization - Cell!lines - Cloning - Specific!cell!types - Cell!separation - Transformed!phenotype - Cytotoxicity - Culture!of!specific!cell!types - Culture!of!tumor!tissue - Three!dimensional!culture!systems.

Human Cell Culture Protocols

Author : Joanna Picot
Publisher : Springer Science & Business Media
ISBN 13 : 1592598617
Total Pages : 357 pages
Book Rating : 4.5/5 (925 download)

DOWNLOAD NOW!

Book Synopsis Human Cell Culture Protocols by : Joanna Picot

Download or read book Human Cell Culture Protocols written by Joanna Picot and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Freshney's Culture of Animal Cells

Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 1119513030
Total Pages : 832 pages
Book Rating : 4.1/5 (195 download)

DOWNLOAD NOW!

Book Synopsis Freshney's Culture of Animal Cells by : R. Ian Freshney

Download or read book Freshney's Culture of Animal Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2021-02-17 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Culture of Animal Cells

Author : R. Ian Freshney
Publisher : Wiley-Liss
ISBN 13 : 9780471348894
Total Pages : 624 pages
Book Rating : 4.3/5 (488 download)

DOWNLOAD NOW!

Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 2000-03-20 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting. The advantages of tissue culture go beyond control of the physiochemical environment and physiological conditions as shown in the comprehensive coverage of tissue culture topics, both organ culture and cell culture, provided in this manual. A wide range of essential information from basic to specialized procedures is presented, highlighting advantages and limitations, and illustrating the properties of different types of culture. This crucial reference for cell culture techniques includes: * New Atlas of Cells section in full-color presentation * Extended coverage of molecular techniques, scale-up, and serum-free medium * New chapter on problem solving * Photographs of cell lines, contaminations, and equipment * Clear and concise tables and charts * Educated recommendations on safety issues, ethical consent, and ownership Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference.

A Manual for Primary Human Cell Culture

Author : Kee Woei Ng
Publisher : World Scientific
ISBN 13 : 9812834788
Total Pages : 213 pages
Book Rating : 4.8/5 (128 download)

DOWNLOAD NOW!

Book Synopsis A Manual for Primary Human Cell Culture by : Kee Woei Ng

Download or read book A Manual for Primary Human Cell Culture written by Kee Woei Ng and published by World Scientific. This book was released on 2010 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.

Laboratory Manual for Human Cell Culture Procedure

Author : Luay Thanoon Younis
Publisher :
ISBN 13 : 9789673634057
Total Pages : 16 pages
Book Rating : 4.6/5 (34 download)

DOWNLOAD NOW!

Book Synopsis Laboratory Manual for Human Cell Culture Procedure by : Luay Thanoon Younis

Download or read book Laboratory Manual for Human Cell Culture Procedure written by Luay Thanoon Younis and published by . This book was released on 2012 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Animal Cell Culture Techniques

Author : Martin Clynes
Publisher : Springer Science & Business Media
ISBN 13 : 3642804128
Total Pages : 624 pages
Book Rating : 4.6/5 (428 download)

DOWNLOAD NOW!

Book Synopsis Animal Cell Culture Techniques by : Martin Clynes

Download or read book Animal Cell Culture Techniques written by Martin Clynes and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Animal Cell Culture

Author : Tajamul Islam Shah
Publisher : Educreation Publishing
ISBN 13 :
Total Pages : 103 pages
Book Rating : 4./5 ( download)

DOWNLOAD NOW!

Book Synopsis Animal Cell Culture by : Tajamul Islam Shah

Download or read book Animal Cell Culture written by Tajamul Islam Shah and published by Educreation Publishing. This book was released on 2018-08-10 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cell and tissue culture has become one of the key and foremost tools used in the life sciences today. It plays pivotal and an enormous role and its applications are increasing day by day at an alarming rate. This hand book serves as a guide and is designed to serve as a basic introduction to animal cell culture. It is a right path for laboratory workers who are using it for the first time, as well as for those who interact with cell culture researchers and who want a better understanding of the key concepts and terminology in this interesting and rapidly growing field. The handbook is lucid, covering topics such as getting familiar with the requirements of a laboratory dedicated to cell culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, freezing, and thawing cultured cells. The information and guidelines presented in the handbook focus on cell lines (finite or continuous) and omit experiments and techniques concerning primary cultures such as isolating and disaggregating tissues. Note: The basics of cell culture experiments share certain similarities, cell culture conditions vary widely for each cell type. Deviating from the culture conditions required for a particular cell type can result in different phenotypes being expressed, therefore you should be familiar with the cell line you are of interest, and closely follow the instructions provided with each product you are using in your experiment.

Basic Cell Culture Protocols

Author : Cheryl D. Helgason
Publisher : Humana Press
ISBN 13 : 9781493956524
Total Pages : 550 pages
Book Rating : 4.9/5 (565 download)

DOWNLOAD NOW!

Book Synopsis Basic Cell Culture Protocols by : Cheryl D. Helgason

Download or read book Basic Cell Culture Protocols written by Cheryl D. Helgason and published by Humana Press. This book was released on 2016-08-23 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Animal Cell Culture

Author : John M. Davis
Publisher : John Wiley & Sons
ISBN 13 : 0470975636
Total Pages : 389 pages
Book Rating : 4.4/5 (79 download)

DOWNLOAD NOW!

Book Synopsis Animal Cell Culture by : John M. Davis

Download or read book Animal Cell Culture written by John M. Davis and published by John Wiley & Sons. This book was released on 2011-03-16 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Cancer Cell Lines Part 1

Author : John R. W. Masters
Publisher : Springer Science & Business Media
ISBN 13 : 0792351436
Total Pages : 295 pages
Book Rating : 4.7/5 (923 download)

DOWNLOAD NOW!

Book Synopsis Cancer Cell Lines Part 1 by : John R. W. Masters

Download or read book Cancer Cell Lines Part 1 written by John R. W. Masters and published by Springer Science & Business Media. This book was released on 1999 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series provides the first complete guide to the culture of human cells. It is a detailed manual and reference resource for researchers in every aspect of medicine, biology, technology and immunobiology using human cells. Every chapter is written by an internationally recognised authority in the field. The first three volumes in the series provide a comprehensive text on the culture of established cell lines from every type of human cancer. They provide a basic manual and reference resource for every cancer research scientist using human cancer cells.

Introduction to Cell and Tissue Culture

Author : Jennie P. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 0585275718
Total Pages : 247 pages
Book Rating : 4.5/5 (852 download)

DOWNLOAD NOW!

Book Synopsis Introduction to Cell and Tissue Culture by : Jennie P. Mather

Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Basic Cell Culture Protocols

Author : Jeffrey W. Pollard
Publisher : Springer Science & Business Media
ISBN 13 : 0896034410
Total Pages : 477 pages
Book Rating : 4.8/5 (96 download)

DOWNLOAD NOW!

Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard

Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Cell Culture

Author :
Publisher : BoD – Books on Demand
ISBN 13 : 1839694459
Total Pages : 156 pages
Book Rating : 4.8/5 (396 download)

DOWNLOAD NOW!

Book Synopsis Cell Culture by :

Download or read book Cell Culture written by and published by BoD – Books on Demand. This book was released on 2022-06-15 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture is cell cloning technology that simulates in vivo environment conditions such as asepsis, appropriate temperature, and pH as well as certain nutritional conditions to enable cells to survive, grow, reproduce, and maintain their structure and function. Cell culture can be used to grow human, animal, plant, and microbial cells. Each type of cell culture has its own characteristics and essential conditions. This book focuses on the advanced technology and applications of cell culture in the research and practice of medical and life sciences. Chapters address such topics as primary cancer cell cultures, 2D and 3D cell cultures, stem cells, nanotechnology, and more.

Cell Culture Technology

Author : Cornelia Kasper
Publisher : Springer
ISBN 13 : 3319748548
Total Pages : 168 pages
Book Rating : 4.3/5 (197 download)

DOWNLOAD NOW!

Book Synopsis Cell Culture Technology by : Cornelia Kasper

Download or read book Cell Culture Technology written by Cornelia Kasper and published by Springer. This book was released on 2018-10-10 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.