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Author : Joseph Sambrook
Publisher :
ISBN 13 : 9780879695774
Total Pages : 0 pages
Book Rating : 4.6/5 (957 download)
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Heather B. Miller
Publisher : Academic Press
ISBN 13 : 0123855454
Total Pages : 227 pages
Book Rating : 4.1/5 (238 download)
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : Joseph Sambrook
Publisher : CSHL Press
ISBN 13 : 9780879695774
Total Pages : 794 pages
Book Rating : 4.6/5 (957 download)
Download or read book Molecular cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2001 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Joseph Sambrook
Publisher :
ISBN 13 :
Total Pages : 690 pages
Book Rating : 4.3/5 (91 download)
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 1989 with total page 690 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Joseph Sambrook
Publisher : CSHL Press
ISBN 13 : 0879697717
Total Pages : 802 pages
Book Rating : 4.8/5 (796 download)
Download or read book The Condensed Protocols from Molecular Cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2006 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Author : Joseph Sambrook
Publisher :
ISBN 13 :
Total Pages : 760 pages
Book Rating : 4.3/5 (91 download)
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2001 with total page 760 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
Author : Melody S. Clark
Publisher : Springer Science & Business Media
ISBN 13 : 3642878733
Total Pages : 551 pages
Book Rating : 4.6/5 (428 download)
Download or read book Plant Molecular Biology — A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author : Joseph Sambrook
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (831 download)
Download or read book Molecular cloning written by Joseph Sambrook and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Stefan Surzycki
Publisher : John Wiley & Sons
ISBN 13 : 0470999241
Total Pages : 246 pages
Book Rating : 4.4/5 (79 download)
Download or read book Human Molecular Biology Laboratory Manual written by Stefan Surzycki and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Author : Joseph Sambrook
Publisher :
ISBN 13 : 9780879695774
Total Pages : 0 pages
Book Rating : 4.6/5 (957 download)
Download or read book Molecular cloning written by Joseph Sambrook and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jennifer A. Doudna
Publisher :
ISBN 13 : 9781621821304
Total Pages : 0 pages
Book Rating : 4.8/5 (213 download)
Download or read book CRISPR-Cas written by Jennifer A. Doudna and published by . This book was released on 2016 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Author : Joseph Sambrook
Publisher :
ISBN 13 : 9780879695774
Total Pages : 0 pages
Book Rating : 4.6/5 (957 download)
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : J. Saxena
Publisher : Scientific Publishers
ISBN 13 : 9386237237
Total Pages : 402 pages
Book Rating : 4.3/5 (862 download)
Download or read book Laboratory Manual of Microbiology, Biochemistry and Molecular Biology written by J. Saxena and published by Scientific Publishers. This book was released on 2015-05-01 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Though many practical books are available in the market but this Laboratory Manual of Microbiology, Biochemistry and Molecular Biology is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.
Author : Judith W. Zyskind
Publisher : Elsevier
ISBN 13 : 1483220974
Total Pages : 210 pages
Book Rating : 4.4/5 (832 download)
Download or read book Recombinant DNA Laboratory Manual written by Judith W. Zyskind and published by Elsevier. This book was released on 2014-05-12 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Author : Joseph Sambrook
Publisher :
ISBN 13 : 9780879698140
Total Pages : 0 pages
Book Rating : 4.6/5 (981 download)
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Erica A. Golemis
Publisher : CSHL Press
ISBN 13 : 0879697237
Total Pages : 954 pages
Book Rating : 4.8/5 (796 download)
Download or read book Protein-protein Interactions written by Erica A. Golemis and published by CSHL Press. This book was released on 2005 with total page 954 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.
Author : K. V. CHAITANYA
Publisher : PHI Learning Pvt. Ltd.
ISBN 13 : 8120348001
Total Pages : 156 pages
Book Rating : 4.1/5 (23 download)
Download or read book CELL AND MOLECULAR BIOLOGY written by K. V. CHAITANYA and published by PHI Learning Pvt. Ltd.. This book was released on 2013-06-21 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.
