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Author : F. Sierra
Publisher : Birkhäuser
ISBN 13 : 3034863837
Total Pages : 145 pages
Book Rating : 4.0/5 (348 download)
Download or read book A Laboratory Guide to In Vitro Transcription written by F. Sierra and published by Birkhäuser. This book was released on 2013-11-21 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : F. Sierra
Publisher : Birkhäuser
ISBN 13 : 9783764323578
Total Pages : 148 pages
Book Rating : 4.3/5 (235 download)
Download or read book A Laboratory Guide to In Vitro Transcription written by F. Sierra and published by Birkhäuser. This book was released on 1990-08-01 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jost
Publisher : Birkhäuser
ISBN 13 : 3034875614
Total Pages : 319 pages
Book Rating : 4.0/5 (348 download)
Download or read book A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions written by Jost and published by Birkhäuser. This book was released on 2013-03-13 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.
Author : Donald Charles Rio
Publisher :
ISBN 13 : 9780879698904
Total Pages : 0 pages
Book Rating : 4.6/5 (989 download)
Download or read book RNA written by Donald Charles Rio and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manualprovides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.
Author : Robert E. Farrell Jr.
Publisher : Elsevier
ISBN 13 : 0080454763
Total Pages : 794 pages
Book Rating : 4.0/5 (84 download)
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Author : Paul A. Krieg
Publisher : John Wiley & Sons
ISBN 13 : 9780471125365
Total Pages : 470 pages
Book Rating : 4.1/5 (253 download)
Download or read book A Laboratory Guide to RNA written by Paul A. Krieg and published by John Wiley & Sons. This book was released on 1996-08-15 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques. Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips. A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms. Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.
Author : Robert E. Farrell Jr.
Publisher : Academic Press
ISBN 13 : 0128046791
Total Pages : 877 pages
Book Rating : 4.1/5 (28 download)
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Academic Press. This book was released on 2017-08-11 with total page 877 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. - Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more - Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies - Presents comprehensive, cutting-edge information covering all aspects of working with RNA - Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application - Presents multiple avenues for addressing the same experimental goals
Author : David K. Gardner
Publisher : Oxford University Press
ISBN 13 : 9780195142266
Total Pages : 434 pages
Book Rating : 4.1/5 (422 download)
Download or read book A Laboratory Guide to the Mammalian Embryo written by David K. Gardner and published by Oxford University Press. This book was released on 2004-01-08 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.
Author : Bozzano G Luisa
Publisher : Academic Press
ISBN 13 : 0323137792
Total Pages : 332 pages
Book Rating : 4.3/5 (231 download)
Download or read book RNA Methodologies written by Bozzano G Luisa and published by Academic Press. This book was released on 2012-12-02 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA.* Studies cellular regulation using RNA as a parameter of Gene Expression* Offers RNA isolation strategies* Explains proper handling, storage, and manipulation of RNA
Author :
Publisher : Academic Press
ISBN 13 : 008085933X
Total Pages : 691 pages
Book Rating : 4.0/5 (88 download)
Download or read book Functional Organization of The Nucleus written by and published by Academic Press. This book was released on 1992-02-03 with total page 691 pages. Available in PDF, EPUB and Kindle. Book excerpt: Functional Organization of The Nucleus
Author : Robert E. Farrell
Publisher : Academic Press
ISBN 13 : 0122496965
Total Pages : 795 pages
Book Rating : 4.1/5 (224 download)
Download or read book RNA Methodologies written by Robert E. Farrell and published by Academic Press. This book was released on 2005-04 with total page 795 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Author : Martin J. Tymms
Publisher : Springer Science & Business Media
ISBN 13 : 1592595243
Total Pages : 426 pages
Book Rating : 4.5/5 (925 download)
Download or read book In Vitro Transcription and Translation Protocols written by Martin J. Tymms and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.
Author :
Publisher :
ISBN 13 :
Total Pages : 320 pages
Book Rating : 4.F/5 ( download)
Download or read book International Journal of Anthropology written by and published by . This book was released on 1993 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : National Library of Medicine (U.S.)
Publisher :
ISBN 13 :
Total Pages : 700 pages
Book Rating : 4.3/5 (91 download)
Download or read book Current Catalog written by National Library of Medicine (U.S.) and published by . This book was released on 1991 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt: First multi-year cumulation covers six years: 1965-70.
Author : Ian R. Phillips
Publisher : Springer Science & Business Media
ISBN 13 : 1592595804
Total Pages : 473 pages
Book Rating : 4.5/5 (925 download)
Download or read book Cytochrome P450 Protocols written by Ian R. Phillips and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques. Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.
Author : G. Dolf
Publisher : Birkhäuser
ISBN 13 : 3034873123
Total Pages : 411 pages
Book Rating : 4.0/5 (348 download)
Download or read book DNA Fingerprinting: Approaches and Applications written by G. Dolf and published by Birkhäuser. This book was released on 2013-03-08 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although DNA fingerprinting is a very young branch of molecular genetics, being barely six years old, its recent impact on science, law and politics has been dramatic. The application of DNA finger printing to forensic and legal medicine has guaranteed a high public profile for this technology, and indeed, scarcely a week goes by with out the press reporting yet another crime successfully solved by molec ular genetics. Less spectacularly, but equally importantly, DNA typing methods are steadily diffusing into an ever wider set of applications and research fields, ranging from medicine through to conservation biology. To date, two DNA fingerprinting workshops have been held in the UK, one in 1988 organised by Terry Burke at the University of Leicester, and the second in 1989 at the University of Nottingham, co-ordinated by David Parkin. In parallel with these workshops, which have provided an important focus for researchers, Bill Amos and Josephine Pemberton in Cambridge have established an informal newsletter "Fingerprint News" which is playing a major role as a forum for DNA fingerprinters. By 1989, it was clear that the field had broadened sufficiently to warrant a full international meeting. As a result, Gaudenz Dolf took on the task of organising the first, of what I hope will be many, International Symposium of DNA Fingerprinting held at Bern during Ist-3rd October 1990. The success of the meeting can be judged from the remarkable attendance, with 270 delegates from no less than 30 countries.
Author : G. P. Rdei
Publisher : World Scientific
ISBN 13 : 9789810227807
Total Pages : 1150 pages
Book Rating : 4.2/5 (278 download)
Download or read book Genetics Manual written by G. P. Rdei and published by World Scientific. This book was released on 1998 with total page 1150 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Redei has created an outstanding compendium of genetics. Arranged as a dictionary, the book is almost an encyclopedic collection of terms & concepts ... The author has managed to define terms with appropriate mixtures of depth & detail for the researcher, along with clarity useful for the nonexpert." Choice, 1998
