3D Electro-Rotation of Single Cells

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Author :
Publisher : Springer Nature
ISBN 13 : 3031016661
Total Pages : 101 pages
Book Rating : 4.0/5 (31 download)

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Book Synopsis 3D Electro-Rotation of Single Cells by : Liang Huang

Download or read book 3D Electro-Rotation of Single Cells written by Liang Huang and published by Springer Nature. This book was released on 2022-05-31 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.

3D Electro-Rotation of Single Cells

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Author :
Publisher : Morgan & Claypool Publishers
ISBN 13 : 1681736926
Total Pages : 121 pages
Book Rating : 4.6/5 (817 download)

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Book Synopsis 3D Electro-Rotation of Single Cells by : Liang Huang

Download or read book 3D Electro-Rotation of Single Cells written by Liang Huang and published by Morgan & Claypool Publishers. This book was released on 2019-12-26 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

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Author :
Publisher : Academic Press
ISBN 13 : 0128142855
Total Pages : 266 pages
Book Rating : 4.1/5 (281 download)

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Book Synopsis Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis by :

Download or read book Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis written by and published by Academic Press. This book was released on 2018-11-22 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Robotics for Cell Manipulation and Characterization

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Author :
Publisher : Elsevier
ISBN 13 : 0323952143
Total Pages : 402 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Robotics for Cell Manipulation and Characterization by : Changsheng Dai

Download or read book Robotics for Cell Manipulation and Characterization written by Changsheng Dai and published by Elsevier. This book was released on 2023-04-20 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: Robotics for Cell Manipulation and Characterization provides fundamental principles underpinning robotic cell manipulation and characterization, state-of-the-art technical advances in micro/nano robotics, new discoveries of cell biology enabled by robotic systems, and their applications in clinical diagnosis and treatment. This book covers several areas, including robotics, control, computer vision, biomedical engineering and life sciences using understandable figures and tables to enhance readers’ comprehension and pinpoint challenges and opportunities for biological and biomedical research. Focuses on, and comprehensively covers, robotics for cell manipulation and characterization Highlights recent advances in cell biology and disease treatment enabled by robotic cell manipulation and characterization Provides insightful outlooks on future challenges and opportunities

Single Cell Analysis in Biotechnology and Systems Biology

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Publisher : MDPI
ISBN 13 : 3038421936
Total Pages : 237 pages
Book Rating : 4.0/5 (384 download)

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Book Synopsis Single Cell Analysis in Biotechnology and Systems Biology by : Fan-Gang Tseng

Download or read book Single Cell Analysis in Biotechnology and Systems Biology written by Fan-Gang Tseng and published by MDPI. This book was released on 2018-10-01 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS

Essentials of Single-Cell Analysis

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Author :
Publisher : Springer
ISBN 13 : 3662491184
Total Pages : 414 pages
Book Rating : 4.6/5 (624 download)

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Book Synopsis Essentials of Single-Cell Analysis by : Fan-Gang Tseng

Download or read book Essentials of Single-Cell Analysis written by Fan-Gang Tseng and published by Springer. This book was released on 2016-01-21 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Nanosensors for Futuristic Smart and Intelligent Healthcare Systems

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Author :
Publisher : CRC Press
ISBN 13 : 1000684245
Total Pages : 414 pages
Book Rating : 4.0/5 (6 download)

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Book Synopsis Nanosensors for Futuristic Smart and Intelligent Healthcare Systems by : Suresh Kaushik

Download or read book Nanosensors for Futuristic Smart and Intelligent Healthcare Systems written by Suresh Kaushik and published by CRC Press. This book was released on 2022-08-18 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book, Nanosensors for Futuristic Smart and Intelligent Healthcare Systems, presents a treatise on nanosensors technology including wearables, implantable devices and wireless tools. The recent pandemic (COVID-19) has changed the behaviour of people towards diagnosis of infectious diseases and monitoring remote patient health status in real-time. The main focus of this book is the basic concepts of nanomaterials and sensing paradigms for medical devices based on nanosensor technology. The book will be valuable to researchers, engineers and scientists interested in the field of healthcare for monitoring health status in real-time.

Cell Viability Assays

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Publisher : Springer Nature
ISBN 13 : 1071630520
Total Pages : 501 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Cell Viability Assays by : Oliver Friedrich

Download or read book Cell Viability Assays written by Oliver Friedrich and published by Springer Nature. This book was released on 2023-05-04 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

Microfluidics for Cells and Other Organisms

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Author :
Publisher : MDPI
ISBN 13 : 3039215620
Total Pages : 200 pages
Book Rating : 4.0/5 (392 download)

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Book Synopsis Microfluidics for Cells and Other Organisms by : Danny van Noort

Download or read book Microfluidics for Cells and Other Organisms written by Danny van Noort and published by MDPI. This book was released on 2019-10-23 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics-based devices play an important role in creating realistic microenvironments in which cell cultures can thrive. They can, for example, be used to monitor drug toxicity and perform medical diagnostics, and be in a static-, perfusion- or droplet-based device. They can also be used to study cell-cell, cell-matrix or cell-surface interactions. Cells can be either single cells, 3D cell cultures or co-cultures. Other organisms could include bacteria, zebra fish embryo, C. elegans, to name a few.

Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms

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Author :
Publisher : Academic Press
ISBN 13 : 0128242434
Total Pages : 406 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms by : Xinyu Liu

Download or read book Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms written by Xinyu Liu and published by Academic Press. This book was released on 2021-08-14 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms provides a comprehensive introduction to the state-of-the-art micro and nano systems that have recently been developed and applied to biophysical studies of cells and small organisms. These micro and nano systems span from microelectromechanical systems (MEMS) and microfluidic devices to robotic micro-nanomanipulation systems. These biophysical studies range from cell mechanics to the neural science of worms and Drosophila. This book will help readers understand the fundamentals surrounding the development of these tools and teach them the most recent advances in cellular and organismal biophysics enabled by these technologies. Comprehensive coverage of micro and nano-system technology and application to biophysical studies of cells and small organisms. Highlights the most recent advances in cellular and organismal biophysics enabled by micro and nano systems. Insightful outlook on future directions and trends in each chapter covering a sub-area of the book topic.

Engineering of Micro/Nano Biosystems

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Author :
Publisher : Springer
ISBN 13 : 9811365490
Total Pages : 368 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Engineering of Micro/Nano Biosystems by : Gregory Barbillon

Download or read book Engineering of Micro/Nano Biosystems written by Gregory Barbillon and published by Springer. This book was released on 2019-08-02 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This tutorial book offers an in-depth overview of the fundamental principles of micro/nano technologies and devices related to sensing, actuation and diagnosis in fluidics and biosystems. Research in the MEMS/NEMS and lab-on-chip fields has seen rapid growth in both academic and industrial domains, as these biodevices and systems are increasingly replacing traditional large size diagnostic tools. This book is unique in describing not only the devices and technologies but also the basic principles of their operation. The comprehensive description of the fabrication, packaging and principles of micro/nano biosystems presented in this book offers guidance for researchers designing and implementing these biosystems across diverse fields including medical, pharmaceutical and biological sciences. The book provides a detailed overview of the fundamental mechanical, optical, electrical and magnetic principles involved, together with the technologies required for the design, fabrication and characterization of micro/nano fluidic systems and bio-devices. Written by a collaborative team from France and Korea, the book is suitable for academics, researchers, advanced level students and industrial manufacturers.

Handbook of Electroporation

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Author :
Publisher : Springer
ISBN 13 : 9783319328850
Total Pages : 0 pages
Book Rating : 4.3/5 (288 download)

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Book Synopsis Handbook of Electroporation by : Damijan Miklavčič

Download or read book Handbook of Electroporation written by Damijan Miklavčič and published by Springer. This book was released on 2017-09-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of 156 chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The Handbook's 276 authors have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook. The book was edited by an international team of Section Editors: P. Thomas Vernier, Boris Rubinsky, Juergen Kolb, Damijan Miklavcic, Marie-Pierre Rols, Javier Raso, Richard Heller, Gregor Serša, Dietrich Knorr, and Eugene Vorobiev.

Advances in Transport Phenomena 2011

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Publisher : Springer Science & Business Media
ISBN 13 : 3319017934
Total Pages : 169 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Advances in Transport Phenomena 2011 by : Liqiu Wang

Download or read book Advances in Transport Phenomena 2011 written by Liqiu Wang and published by Springer Science & Business Media. This book was released on 2013-10-07 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of the annual review “Advances in Transport Phenomena” series contains three in-depth review articles on the microfluidic fabrication of vesicles, the dielectrophoresis field-flow fractionation for continuous-flow separation of particles and cells in microfluidic devices, and the thermodynamic analysis and optimization of heat exchangers, respectively.

Encyclopedia of Food Microbiology

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Publisher : Academic Press
ISBN 13 : 0123847338
Total Pages : 3243 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Encyclopedia of Food Microbiology by : Carl A. Batt

Download or read book Encyclopedia of Food Microbiology written by Carl A. Batt and published by Academic Press. This book was released on 2014-04-02 with total page 3243 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the world's leading scientists and spanning over 400 articles in three volumes, the Encyclopedia of Food Microbiology, Second Edition is a complete, highly structured guide to current knowledge in the field. Fully revised and updated, this encyclopedia reflects the key advances in the field since the first edition was published in 1999 The articles in this key work, heavily illustrated and fully revised since the first edition in 1999, highlight advances in areas such as genomics and food safety to bring users up-to-date on microorganisms in foods. Topics such as DNA sequencing and E. coli are particularly well covered. With lists of further reading to help users explore topics in depth, this resource will enrich scientists at every level in academia and industry, providing fundamental information as well as explaining state-of-the-art scientific discoveries. This book is designed to allow disparate approaches (from farmers to processors to food handlers and consumers) and interests to access accurate and objective information about the microbiology of foods Microbiology impacts the safe presentation of food. From harvest and storage to determination of shelf-life, to presentation and consumption. This work highlights the risks of microbial contamination and is an invaluable go-to guide for anyone working in Food Health and Safety Has a two-fold industry appeal (1) those developing new functional food products and (2) to all corporations concerned about the potential hazards of microbes in their food products

Comprehensive Biotechnology

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Publisher : Elsevier
ISBN 13 : 0444640479
Total Pages : 4876 pages
Book Rating : 4.4/5 (446 download)

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Book Synopsis Comprehensive Biotechnology by :

Download or read book Comprehensive Biotechnology written by and published by Elsevier. This book was released on 2019-07-17 with total page 4876 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Biotechnology, Third Edition, Six Volume Set unifies, in a single source, a huge amount of information in this growing field. The book covers scientific fundamentals, along with engineering considerations and applications in industry, agriculture, medicine, the environment and socio-economics, including the related government regulatory overviews. This new edition builds on the solid basis provided by previous editions, incorporating all recent advances in the field since the second edition was published in 2011. Offers researchers a one-stop shop for information on the subject of biotechnology Provides in-depth treatment of relevant topics from recognized authorities, including the contributions of a Nobel laureate Presents the perspective of researchers in different fields, such as biochemistry, agriculture, engineering, biomedicine and environmental science

Zell's Popular Encyclopedia

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Publisher :
ISBN 13 :
Total Pages : 650 pages
Book Rating : 4.:/5 (31 download)

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Book Synopsis Zell's Popular Encyclopedia by :

Download or read book Zell's Popular Encyclopedia written by and published by . This book was released on 1882 with total page 650 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Optical Trapping and Manipulation

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Publisher : MDPI
ISBN 13 : 303943537X
Total Pages : 128 pages
Book Rating : 4.0/5 (394 download)

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Book Synopsis Optical Trapping and Manipulation by : Philip Jones

Download or read book Optical Trapping and Manipulation written by Philip Jones and published by MDPI. This book was released on 2020-11-25 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present “Optical Trapping and Manipulation: From Fundamentals to Applications”, a Special Issue of Micromachines dedicated to the latest research in optical trapping. In recognition of the broad impact of optical manipulation techniques across disciplines, this Special Issue collected contributions related to all aspects of optical trapping and manipulation. Both theoretical and experimental studies were welcome, and applications of optical manipulation methods in fields including (but not limited to) single molecule biophysics, cell biology, nanotechnology, atmospheric chemistry, and fundamental optics were particularly welcome in order to showcase the breadth of the current research. The Special Issue accepted diverse forms of contributions, including research papers, short communications, methods, and review articles representing the state-of-the-art in optical trapping.