Xenopus laevis: Practical Uses in Cell and Molecular Biology

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Publisher : Academic Press
ISBN 13 : 0080859348
Total Pages : 757 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Xenopus laevis: Practical Uses in Cell and Molecular Biology by :

Download or read book Xenopus laevis: Practical Uses in Cell and Molecular Biology written by and published by Academic Press. This book was released on 1992-02-03 with total page 757 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all laboratories that use Xenopus. * Features approximately twenty full-color plates illustrating experimental techniques and results and depicting embryonic development * Provides complete coverage of Xenopus laevis as an experimental system including * Embryonic development, genetics, and laboratory care * Up-to-date protocols for experimental techniques using oocytes and embryos * General information listing recipes, suppliers, sequences, codons, and clones

Methods in Cell Biology

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Publisher : Academic Press
ISBN 13 : 9780125641364
Total Pages : 646 pages
Book Rating : 4.6/5 (413 download)

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Book Synopsis Methods in Cell Biology by : Brian K. Kay

Download or read book Methods in Cell Biology written by Brian K. Kay and published by Academic Press. This book was released on 1991-12-17 with total page 646 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all laboratories that use Xenopus. * Features approximately twenty full-color plates illustrating experimental techniques and results and depicting embryonic development * Provides complete coverage of Xenopus laevis as an experimental system including * Embryonic development, genetics, and laboratory care * Up-to-date protocols for experimental techniques using oocytes and embryos * General information listing recipes, suppliers, sequences, codons, and clones

Methods in Cell Biology

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Publisher :
ISBN 13 : 9780125641364
Total Pages : 0 pages
Book Rating : 4.6/5 (413 download)

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Book Synopsis Methods in Cell Biology by : Brian K. Kay

Download or read book Methods in Cell Biology written by Brian K. Kay and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the first comprehensive treatment of the wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology. Each chapter includes background information, experimental protocols, and suggested applications. An extensive array of techniques is featured. The authors are experienced researchers who have written chapters that will be useful to both experienced researchers and to those new to Xenopus as an experimental system. Full-color plates and diagrams enhance the educational value of this book, which provides a valuable permanent resource for all labo.

Xenopus Protocols

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1597450006
Total Pages : 487 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Xenopus Protocols by : X. Johné Liu

Download or read book Xenopus Protocols written by X. Johné Liu and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.

Molecular Methods in Developmental Biology

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Publisher : Springer Science & Business Media
ISBN 13 : 1592596789
Total Pages : 222 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Molecular Methods in Developmental Biology by : Matt Guille

Download or read book Molecular Methods in Developmental Biology written by Matt Guille and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.

Xenopus Development

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 111849282X
Total Pages : 459 pages
Book Rating : 4.1/5 (184 download)

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Book Synopsis Xenopus Development by : Malgorzata Kloc

Download or read book Xenopus Development written by Malgorzata Kloc and published by John Wiley & Sons. This book was released on 2014-03-19 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: Frogs from the genus Xenopus have long been used as model organisms in basic and biomedical research. These frogs have helped unlock key fundamental developmental and cellular processes that have led to important scientific breakthroughs and have had practical application in embryology, cancer research and regenerative medicine. Xenopus Development is a vital resource on the biology and development of these key model organisms, and will be a great tool to researchers using these frogs in various disciplines of biological science. Xenopus Development is divided into four sections, the first three highlight key processes in Xenopus development from embryo to metamophosis. These sections focus on the cellular processes, organogenesis and embryo development. The final section highlights novel techniques and approaches being used in Xenopus research. Providing thorough and detailed coverage, Xenopus Development, will be a timely and welcome volume for those working in cell and molecular biology, genetics, developmental biology and biomedical research. Provides broad overview of the developmental biology of both Xenopus laevis and Xenopus tropicalis Explores cellular to systems development in key biomedical model organisms Timely synthesis of the field of Xenopus biology Highlights key biomedical and basic biological findings unlocked by Xenopus

Xenopus

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Author :
Publisher : CRC Press
ISBN 13 : 1000529819
Total Pages : 768 pages
Book Rating : 4.0/5 (5 download)

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Book Synopsis Xenopus by : Abraham Fainsod

Download or read book Xenopus written by Abraham Fainsod and published by CRC Press. This book was released on 2022-04-19 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the amphibian, Xenopus, one of the most commonly used model animals in the biological sciences. Over the past 50 years, the use of Xenopus has made possible many fundamental contributions to our knowledge in cell biology, developmental biology, molecular biology, and neurobiology. In recent years, with the completion of the genome sequence of the main two species and the application of genome editing techniques, Xenopus has emerged as a powerful system to study fundamental disease mechanisms and test treatment possibilities. Xenopus has proven an essential vertebrate model system for understanding fundamental cell and developmental biological mechanisms, for applying fundamental knowledge to pathological processes, for deciphering the function of human disease genes, and for understanding genome evolution. Key Features Provides historical context of the contributions of the model system Includes contributions from an international team of leading scholars Presents topics spanning cell biology, developmental biology, genomics, and disease model Describes recent experimental advances Incorporates richly illustrated diagrams and color images Related Titles Green, S. L. The Laboratory Xenopus sp. (ISBN 978-1-4200-9109-0) Faber, J. & P. D. Nieuwkoop. Normal Table of Xenopus laevis (Daudin): A Systematical & Chronological Survey of the Development from the Fertilized Egg till the End of Metamorphosis (ISBN 978-0-8153-1896-5) Jarret, R. L. & K. McCluskey. The Biological Resources of Model Organisms (ISBN 978-1-0320-9095-5) The Open Access version of this book, available at www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license.

Xenopus—Advances in Research and Application: 2012 Edition

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Author :
Publisher : ScholarlyEditions
ISBN 13 : 1481620908
Total Pages : 39 pages
Book Rating : 4.4/5 (816 download)

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Book Synopsis Xenopus—Advances in Research and Application: 2012 Edition by :

Download or read book Xenopus—Advances in Research and Application: 2012 Edition written by and published by ScholarlyEditions. This book was released on 2012-12-26 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: Xenopus—Advances in Research and Application: 2012 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Xenopus in a concise format. The editors have built Xenopus—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Xenopus in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Xenopus—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Xenopus

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Publisher : Humana
ISBN 13 : 9781493987832
Total Pages : 0 pages
Book Rating : 4.9/5 (878 download)

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Book Synopsis Xenopus by : Kris Vleminckx

Download or read book Xenopus written by Kris Vleminckx and published by Humana. This book was released on 2018-08-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.

Cell Cycle - Materials and Methods

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Publisher : Springer Science & Business Media
ISBN 13 : 3642577830
Total Pages : 312 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Cell Cycle - Materials and Methods by : Michele Pagano

Download or read book Cell Cycle - Materials and Methods written by Michele Pagano and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual. A glossary helps the reader to cope with the complex cell cycle terminology.

Early Development of Xenopus Laevis

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Publisher :
ISBN 13 : 9780879695781
Total Pages : 0 pages
Book Rating : 4.6/5 (957 download)

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Book Synopsis Early Development of Xenopus Laevis by : Hazel L. Sive

Download or read book Early Development of Xenopus Laevis written by Hazel L. Sive and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.

Drosophila melanogaster: Practical Uses in Cell and Molecular Biology

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Publisher : Academic Press
ISBN 13 : 0080859429
Total Pages : 789 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Drosophila melanogaster: Practical Uses in Cell and Molecular Biology by :

Download or read book Drosophila melanogaster: Practical Uses in Cell and Molecular Biology written by and published by Academic Press. This book was released on 1995-01-25 with total page 789 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future. Key Features* Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster* Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques

Atlas of Xenopus Development

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Publisher : Springer Science & Business Media
ISBN 13 : 8847029058
Total Pages : 134 pages
Book Rating : 4.8/5 (47 download)

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Book Synopsis Atlas of Xenopus Development by : G. Bernardini

Download or read book Atlas of Xenopus Development written by G. Bernardini and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developmental biology attracts scientists from many different areas of biology, and the amphibian Xenopus holds a special place among the organisms studied as a model of vertebrate development. Until now, no recently published atlas existed to aid researchers and students coming to the Xenopus embryo for the first time. The present book satisfies this need. With its synthesizing approach and its generous provision of beautiful scanning, transmission, and light microscopy images, this unique volume will be a standard reference not only for developmental biologists but for all students of embryology, histology, and comparative anatomy.

Experimental Design for Biologists

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Publisher : CSHL Press
ISBN 13 : 0879697350
Total Pages : 211 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Experimental Design for Biologists by : David J. Glass

Download or read book Experimental Design for Biologists written by David J. Glass and published by CSHL Press. This book was released on 2007 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well-received course taught by the author, Experimental Design for Biologistsfills this gap. Experimental Design for Biologistsexplains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls†and “experimentalist controls†. Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls†. Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologistsis an essential source of theory and practical guidance in designing a research plan.

Intermediate Filaments

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Publisher : Springer Science & Business Media
ISBN 13 : 9780306458545
Total Pages : 660 pages
Book Rating : 4.4/5 (585 download)

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Book Synopsis Intermediate Filaments by : Harald Herrmann

Download or read book Intermediate Filaments written by Harald Herrmann and published by Springer Science & Business Media. This book was released on 1998-09-30 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprises 20 contributions on intermediate filament (IF) proteins, providing a comprehensive overview of the essentials of IF research and very recent developments, including many previously unpublished concepts and results. Representative topics include intermediate filament organization during oogenesis and early development in the clawed frog, lessons from keratin transgenic and knockout mice, cytokeratins as markers of differentiation in the diagnosis of epithelial tumors, vimentin and lipid metabolism, and nuclear lamin- binding proteins. Annotation copyrighted by Book News, Inc., Portland, OR

Methods in Plant Cell Biology, Part B

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Author :
Publisher : Academic Press
ISBN 13 : 0080859488
Total Pages : 581 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Methods in Plant Cell Biology, Part B by :

Download or read book Methods in Plant Cell Biology, Part B written by and published by Academic Press. This book was released on 1995-10-10 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Methods in Plant Cell Biology, Part A

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Author :
Publisher : Elsevier
ISBN 13 : 008085947X
Total Pages : 605 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Methods in Plant Cell Biology, Part A by :

Download or read book Methods in Plant Cell Biology, Part A written by and published by Elsevier. This book was released on 1995-10-10 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants