Virus Induced Gene Silencing

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Virus-Induced Gene Silencing

Author : Annette Becker
Publisher : Humana Press
ISBN 13 : 9781627032773
Total Pages : 0 pages
Book Rating : 4.0/5 (327 download)

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Book Synopsis Virus-Induced Gene Silencing by : Annette Becker

Download or read book Virus-Induced Gene Silencing written by Annette Becker and published by Humana Press. This book was released on 2013-02-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.

Virus-Induced Gene Silencing in Plants

Author : Vincent Courdavault
Publisher :
ISBN 13 : 9781071607510
Total Pages : 261 pages
Book Rating : 4.6/5 (75 download)

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Book Synopsis Virus-Induced Gene Silencing in Plants by : Vincent Courdavault

Download or read book Virus-Induced Gene Silencing in Plants written by Vincent Courdavault and published by . This book was released on 2020 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Plant Functional Genomics

Author : Erich Grotewold
Publisher : Springer Science & Business Media
ISBN 13 : 1592594131
Total Pages : 443 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Functional Genomics by : Erich Grotewold

Download or read book Plant Functional Genomics written by Erich Grotewold and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 443 pages. Available in PDF, EPUB and Kindle. Book excerpt: Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.

Phytonutritional Improvement of Crops

Author : Noureddine Benkeblia
Publisher : John Wiley & Sons
ISBN 13 : 1119079942
Total Pages : 549 pages
Book Rating : 4.1/5 (19 download)

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Book Synopsis Phytonutritional Improvement of Crops by : Noureddine Benkeblia

Download or read book Phytonutritional Improvement of Crops written by Noureddine Benkeblia and published by John Wiley & Sons. This book was released on 2017-10-02 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: An in-depth treatment of cutting-edge work being done internationally to develop new techniques in crop nutritional quality improvement Phytonutritional Improvement of Crops explores recent advances in biotechnological methods for the nutritional enrichment of food crops. Featuring contributions from an international group of experts in the field, it provides cutting-edge information on techniques of immense importance to academic, professional and commercial operations. World population is now estimated to be 7.5 billion people, with an annual growth rate of nearly 1.5%. Clearly, the need to enhance not only the quantity of food produced but its quality has never been greater, especially among less developed nations. Genetic manipulation offers the best prospect for achieving that goal. As many fruit crops provide proven health benefits, research efforts need to be focused on improving the nutritional qualities of fruits and vegetables through increased synthesis of lycopene and beta carotene, anthocyanins and some phenolics known to be strong antioxidants. Despite tremendous growth in the area occurring over the past several decades, the work has only just begun. This book represents an effort to address the urgent need to promote those efforts and to mobilise the tools of biotechnical and genetic engineering of the major food crops. Topics covered include: New applications of RNA-interference and virus induced gene silencing (VIGS) for nutritional genomics in crop plants Biotechnological techniques for enhancing carotenoid in crops and their implications for both human health and sustainable development Progress being made in the enrichment and metabolic profiling of diverse carotenoids in a range of fruit crops, including tomatoes, sweet potatoes and tropical fruits Biotechnologies for boosting the phytonutritional values of key crops, including grapes and sweet potatoes Recent progress in the development of transgenic rice engineered to massively accumulate flavonoids in-seed Phytonutritional Improvement of Crops is an important text/reference that belongs in all universities and research establishments where agriculture, horticulture, biological sciences, and food science and technology are studied, taught and applied.

100 Years of Virology

Author : Charles H. Calisher
Publisher : Springer Science & Business Media
ISBN 13 : 3709164257
Total Pages : 222 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis 100 Years of Virology by : Charles H. Calisher

Download or read book 100 Years of Virology written by Charles H. Calisher and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: One hundred years ago, when Martinus W. Beijerinck in Delft and Friedrich Loeffler on Riems Island discovered a new class of infectious agents in plants and animals, a new discipline was born. This book, a compilation of papers written by well-recognized scientists, gives an impression of the early days, the pioneer period and the current state of virology. Recent developments and future perspectives of this discipline are sketched against a historic background. With contributions by A. Alcami, D. Baulcombe, F. Brown, L. W. Enquist, H. Feldmann, A. Garcia-Sastre, D. Griffiths, M. C. Horzinek, A. van Kammen, H.-D. Klenk, F. A. Murphy, T. Muster, R. O'Neill, P. Palese, C. Patience, R. Rott, H.- P. Schmiedebach, S. Schneider-Schaulies, G. L. Smith, J. A. Symons, Y. Takeuchi, V. ter Meulen, P. J. W. Venables, V. E. Volchkov, V. A. Volchkova, R. A. Weiss, W. Wittmann, H. Zheng.

Plant Gene Silencing

Author : M.A. Matzke
Publisher : Springer Science & Business Media
ISBN 13 : 9401141835
Total Pages : 297 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Plant Gene Silencing by : M.A. Matzke

Download or read book Plant Gene Silencing written by M.A. Matzke and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an up-to-date and comprehensive collection of reviews on various aspects of epigenetic gene silencing in plants. Research on this topic has undergone explosive growth during the past decade and has revealed novel features of gene regulation and plant defense responses that also apply to animals and fungi. Gene silencing is relevant for agricultural biotechnology because stable expression of transgenes is required for the successful commercialization of genetically engineered crops. The reviews have been written by distinguished authors who have made significant contributions to plant gene silencing research. This volume supersedes other books on gene silencing by focussing on plant systems, where many pioneering experiments have been performed, and by including the latest developments from top laboratories. The book is geared toward advanced students of genetics and plant sciences as well as applied and basic research scientists who work with transgenic organisms and epigenetic regulation of gene expression.

Orchid Biotechnology II

Author : Hong-Hwa Chen
Publisher : World Scientific
ISBN 13 : 981432793X
Total Pages : 380 pages
Book Rating : 4.8/5 (143 download)

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Book Synopsis Orchid Biotechnology II by : Hong-Hwa Chen

Download or read book Orchid Biotechnology II written by Hong-Hwa Chen and published by World Scientific. This book was released on 2011 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: Orchid Biotechnology II presents a series of recent works on both basic and applied researches in biotechnology progress for Phalaenopsis and Oncidium orchids. These include the development of flower, ovule, gynostemium and perianth, the discovery of new orchid-infecting viruses and virus movement, secondary metabolites, technology of DNA endoduplication and genetic transformation, growth regulation by micronutrition and orchid mycorrhiza, and plant growth substances for flowering. The diversity and specialization in orchid floral morphology have fascinated botanists and collectors for centuries. The orchid industry has been growing substantially in the past ten years worldwide. This book focuses on the recent advances in the research of orchid biotechnology from the past ten years in Taiwan. To advance the orchid industry, enhancement of basic research as well as advanced biotechnology will provide a good platform to improve flower quality and breeding of new varieties.

Plant Gene Silencing

Author : Kirankumar S. Mysore
Publisher : Humana
ISBN 13 : 9781493924523
Total Pages : 0 pages
Book Rating : 4.9/5 (245 download)

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Book Synopsis Plant Gene Silencing by : Kirankumar S. Mysore

Download or read book Plant Gene Silencing written by Kirankumar S. Mysore and published by Humana. This book was released on 2015-03-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.

Human Herpesviruses

Author : Ann Arvin
Publisher : Cambridge University Press
ISBN 13 : 1139461648
Total Pages : 1325 pages
Book Rating : 4.1/5 (394 download)

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Book Synopsis Human Herpesviruses by : Ann Arvin

Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.

Barley

Author : Wendy A. Harwood
Publisher : Humana Press
ISBN 13 : 9781493989423
Total Pages : 359 pages
Book Rating : 4.9/5 (894 download)

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Book Synopsis Barley by : Wendy A. Harwood

Download or read book Barley written by Wendy A. Harwood and published by Humana Press. This book was released on 2018-12-02 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

Natural Product Biosynthesis

Author : Christopher T. Walsh
Publisher : Royal Society of Chemistry
ISBN 13 : 1788010760
Total Pages : 787 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Natural Product Biosynthesis by : Christopher T. Walsh

Download or read book Natural Product Biosynthesis written by Christopher T. Walsh and published by Royal Society of Chemistry. This book was released on 2017-04-28 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt: This textbook describes the types of natural products, the biosynthetic pathways that enable the production of these molecules, and an update on the discovery of novel products in the post-genomic era.

Plant Resistance to Viruses

Author : David Evered
Publisher : John Wiley & Sons
ISBN 13 : 0470513578
Total Pages : 226 pages
Book Rating : 4.4/5 (75 download)

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Book Synopsis Plant Resistance to Viruses by : David Evered

Download or read book Plant Resistance to Viruses written by David Evered and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concern about the environmental consequences of the widespread use of pesticides has increased, and evidence of pesticide-resistant virus vectors have continued to emerge. This volume presents a timely survey of the mechanisms of plant resistance and examines current developments in breeding for resistance, with particular emphasis on advances in genetic engineering which allow for the incorporation of viral genetic material into plants. Discusses the mechanisms of innate resistance in strains of tobacco, tomato, and cowpea; various aspects of induced resistance, including the characterization and roles of the pathogenesis-related proteins; antiviral substances and their comparison with interferon; and cross-protection between plant virus strains. Also presents several papers which evaluate the status of genetic engineering as it relates to breeding resistant plants. Among these are discussions of the potential use of plant viruses as gene vectors, gene coding for viral coat protein, satellite RNA, and antisense RNA, and practical issues such as the durability of resistant crop plants in the field.

Plant-Pathogen Interactions

Author : Pamela C. Ronald
Publisher : Springer Science & Business Media
ISBN 13 : 1592599664
Total Pages : 253 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant-Pathogen Interactions by : Pamela C. Ronald

Download or read book Plant-Pathogen Interactions written by Pamela C. Ronald and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts. This will help to develop better methods for disease control in plants and animals.

Plant Virology

Author : Aiming Wang
Publisher : Humana
ISBN 13 : 9781071618370
Total Pages : 0 pages
Book Rating : 4.6/5 (183 download)

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Book Synopsis Plant Virology by : Aiming Wang

Download or read book Plant Virology written by Aiming Wang and published by Humana. This book was released on 2022-12-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.

RNA Interference

Author : Dr. Tim Doran
Publisher : CABI
ISBN 13 : 1845934105
Total Pages : 269 pages
Book Rating : 4.8/5 (459 download)

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Book Synopsis RNA Interference by : Dr. Tim Doran

Download or read book RNA Interference written by Dr. Tim Doran and published by CABI. This book was released on 2009 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.

Transgenic Crops VI

Author : Eng Chong Pua
Publisher : Springer Science & Business Media
ISBN 13 : 3540717110
Total Pages : 442 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Transgenic Crops VI by : Eng Chong Pua

Download or read book Transgenic Crops VI written by Eng Chong Pua and published by Springer Science & Business Media. This book was released on 2007-09-07 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume, Transgenic Crops VI, includes the following broad topic sections: Oils and Fibers, Medicinal Crops, Ornamental Crops, Forages and Grains, Regulatory and Intellectual Property of Genetically Manipulated Plants. It is an invaluable reference for plant breeders, researchers and graduate students in the fields of plant biotechnology, agronomy, horticulture, forestry, genetics, and both plant cell and molecular biology.

Plant Virology

Author : R. E. F. Matthews
Publisher : Academic Press
ISBN 13 : 1483258661
Total Pages : 916 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Plant Virology by : R. E. F. Matthews

Download or read book Plant Virology written by R. E. F. Matthews and published by Academic Press. This book was released on 2013-10-22 with total page 916 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Virology, Second Edition, was written to cover the substantial developments in many areas of plant virology since the first edition was published. Advances have been made in all branches of the subject, but these have been most far reaching with respect to the structure of viruses and of their components, and in the understanding of how viral genomes are organized and how viruses replicate in cells. Significant developments have also occurred in the understanding of how viruses are transmitted by invertebrates and in the application of control measures for specific diseases. The taxonomy of viruses has advanced significantly, and there are now 25 internationally approved families and groups of plant viruses. All these developments have required that most sections be entirely rewritten. This book is intended primarily for graduate students in plant pathology, plant virology, general virology, and microbiology, and for teachers and research workers in these fields. It should also prove useful to some people in related disciplines—molecular biologists, biochemists, plant physiologists, and entomologists.