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Unconventional Electron Microscopy For Molecular Structure Determination
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Book Synopsis Unconventional Electron Microscopy for Molecular Structure Determination by : W. Hoppe
Download or read book Unconventional Electron Microscopy for Molecular Structure Determination written by W. Hoppe and published by Springer-Verlag. This book was released on 2013-07-02 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Generally it is not sufficiently appreciated that electron microscopy is in fact a diffraction method. In essential aspects electron microscopes are more closely related to X-ray diffracto· meters than to light microscopes. In electron microscopes monochromatized radiation and coherent illumination (never used in light microscopy) correspond in X-ray diffractometers to the primary beam with a small divergence. Imaging ina general sense can take place in interference experiments between a primary beam and a scattered beam, or between diffe rent deflected scattered beams. This leads to the realization of an old dream in diffracto metry, namely to a general experimental solution of the "phase problem". The most im pressive analogy, however, concerns the potential of the electron microscope as a tool for structure determination (where the radiation wavelenght is smaller than the atomic distan ces). It was therefore considered timely to treat this topic in this series. It was a fortunate cioncidence that in 1976 a Workshop on "Unconventional Electron Microscope Methods for the Investigation of Molecular Structures" (sponsored by the European Molecular Biology Organisation, the Deutsche Forschungsgemeinschaft and the Max-Planck-Gesell schaft) took place, and that most speakers presenting introductory lectures agreed to publish their contributions in an expanded version in this volume. This volume is thus not a symposium report in the usual sense since it contains the majority of these introductory lectures only.
Book Synopsis Cryo-EM Part B: 3-D Reconstruction by :
Download or read book Cryo-EM Part B: 3-D Reconstruction written by and published by Academic Press. This book was released on 2010-09-30 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
Book Synopsis Electron Microscopy at Molecular Dimensions by : W. Baumeister
Download or read book Electron Microscopy at Molecular Dimensions written by W. Baumeister and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: If, ten years ago, one had been asked to comment on the prospects of peering into the fmest details of biomolecular organization, most electron microscopists would, I suppose at least, have been quite en thusiastic. When, during the early seventies, several groups were success ful in visualizing single heavy atoms, which undoubtedly was a techni cal triumph, this prompted the most sanguine expectations among bi ologists. In the following years, however, it began to transpire that radiation damage might impose limitations preventing us from taking full advantage of these exciting instrumental feasibilities. Fortunately, the radiation damage nightmare did no paralyze further activities, and it was in particular the work on the purple membrane which, brilliant ly exploiting the redundancy stratagem, revealed exhilarating new perspectives. Now, almost five years later, it seemed timely and appro priate to organize an international symposium to discuss and weight recent activities and current trends in "molecular microscopy". In planning this symposium, we selected topics according to our view of what is important or will deserve more attention in the near future. Taking into consideration suggestions made by the invited participants, some supplementary aspects were included; as a conse quence, the program developed somewhat beyond the scope as adum brated by the original title of this meeting (Regular 2-D Arrays of Biomacromolecules: Structure Determination and Assembly). As the meeting was organized, we had three morning sessions aimed at reflecting the "State ofthe Art".
Book Synopsis Electron Microscopy at Molecular Dimensions by :
Download or read book Electron Microscopy at Molecular Dimensions written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Springer Handbook of Microscopy by : Peter W. Hawkes
Download or read book Springer Handbook of Microscopy written by Peter W. Hawkes and published by Springer Nature. This book was released on 2019-11-02 with total page 1561 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features reviews by leading experts on the methods and applications of modern forms of microscopy. The recent awards of Nobel Prizes awarded for super-resolution optical microscopy and cryo-electron microscopy have demonstrated the rich scientific opportunities for research in novel microscopies. Earlier Nobel Prizes for electron microscopy (the instrument itself and applications to biology), scanning probe microscopy and holography are a reminder of the central role of microscopy in modern science, from the study of nanostructures in materials science, physics and chemistry to structural biology. Separate chapters are devoted to confocal, fluorescent and related novel optical microscopies, coherent diffractive imaging, scanning probe microscopy, transmission electron microscopy in all its modes from aberration corrected and analytical to in-situ and time-resolved, low energy electron microscopy, photoelectron microscopy, cryo-electron microscopy in biology, and also ion microscopy. In addition to serving as an essential reference for researchers and teachers in the fields such as materials science, condensed matter physics, solid-state chemistry, structural biology and the molecular sciences generally, the Springer Handbook of Microscopy is a unified, coherent and pedagogically attractive text for advanced students who need an authoritative yet accessible guide to the science and practice of microscopy.
Book Synopsis Analytical Methods in Supramolecular Chemistry by : Christoph A. Schalley
Download or read book Analytical Methods in Supramolecular Chemistry written by Christoph A. Schalley and published by John Wiley & Sons. This book was released on 2012-09-25 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of "Analytical Methods in Supramolecular Chemistry" comes in two volumes and covers a broad range of modern methods and techniques now used for investigating supramolecular systems, e. g. NMR spectroscopy, mass spectrometry, extraction methods, crystallography, single molecule spectroscopy, electrochemisty, and many more. In this second edition, tutorial inserts have been introduced, making the book also suitable as supplementary reading for courses on supramolecular chemistry. All chapters have been revised and updated and four new chapters have been added. A must-have handbook for Organic and Analytical Chemists, Spectroscopists, Materials Scientists, and Ph.D. Students in Chemistry. From reviews of the first edition: "This timely book should have its place in laboratories dealing with supramolecular objects. It will be a source of reference for graduate students and more experienced researchers and could induce new ideas on the use of techniques other than those usually used in the laboratory." Journal of the American Chemical Society (2008) VOL. 130, NO. 1 doi: 10.1021/ja0769649 "The book as a whole or single chapters will stimulate the reader to widen his horizon in chemistry and will help him to have new ideas in his research." Anal Bioanal Chem (2007) 389:2039-2040 DOI: 10.1007/s00216-007-1677-1
Book Synopsis Low-Temperature Microscopy and Analysis by : Patrick Echlin
Download or read book Low-Temperature Microscopy and Analysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 1992-03-31 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive volume explores the preparation, examination, and analysis of organic, hydrated, and biological specimens using cryomicroscopic techniques. More than 200 illustrations supplement the text.
Author :Rudolf A. Steinbrecht Publisher :Springer Science & Business Media ISBN 13 :3642728154 Total Pages :308 pages Book Rating :4.6/5 (427 download)
Book Synopsis Cryotechniques in Biological Electron Microscopy by : Rudolf A. Steinbrecht
Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Book Synopsis Advances in Imaging and Electron Physics by :
Download or read book Advances in Imaging and Electron Physics written by and published by Academic Press. This book was released on 2009-11-05 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Imaging and Electron Physics merges two long-running serials--Advances in Electronics and Electron Physics and Advances in Optical and Electron Microscopy. This series features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science and digital image processing, electromagnetic wave propagation, electron microscopy, and the computing methods used in all these domains. This particular volume presents several timely articles on the scanning transmission electron microscope. - Updated with contributions from leading international scholars and industry experts - Discusses hot topic areas and presents current and future research trends - Provides an invaluable reference and guide for physicists, engineers and mathematicians
Download or read book Cellular Imaging written by Eric Hanssen and published by Springer. This book was released on 2017-12-30 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Book Synopsis Single-particle Cryo-electron Microscopy by : Joachim Frank
Download or read book Single-particle Cryo-electron Microscopy written by Joachim Frank and published by World Scientific Publishing Company. This book was released on 2017-12-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.
Book Synopsis Analytical Methods High-Melting Metals by :
Download or read book Analytical Methods High-Melting Metals written by and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: In solid state physics and in materials science the investigation of the connection between the properties of solids and their microstructure is of major importance. For crystalline materials this connection is related to the lattice structure, and it can be shown convinc ingly that the material properties depend on deviations from the ideal lattice structure in the majority of cases. For this reason a reliable detection and analysis of defects in "nearly perfect" crystals is necessary, and a sufficient spatial resolution of the methods applied is required. Because electrons on the one hand strongly interact with the matter to be investigated and on the other hand can easily be focused electron-optical methods are very advantageous for this purpose. They are used in the diffraction mode, in the imaging mode and in the spectroscopic mode. The attainable high lateral resolution in the imaging mode makes the application of electron microscopy especially effective. Although already valuable information on crystal defects can be gained by using the routine technique of diffraction contrast imagingl-3) which has a resolution of some 4 10 nm - in the special weak-beam technique ) of some nm -, the detection of crystal defects and inhomogeneities, resp. on an atomic or molecular level by the aid of high resolution electron microscopy gets increasing importance.
Book Synopsis Transmission Electron Microscopy by : Ludwig Reimer
Download or read book Transmission Electron Microscopy written by Ludwig Reimer and published by Springer. This book was released on 2013-11-11 with total page 558 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hier steht der Extremkurztext.
Book Synopsis Protein Structure Determination on Lipid Substrates by Electron Microscopy by : Elizabeth Morrison Wilson-Kubalek
Download or read book Protein Structure Determination on Lipid Substrates by Electron Microscopy written by Elizabeth Morrison Wilson-Kubalek and published by . This book was released on 2005 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the impressive growth in gene sequence data and advances in genetics the number of proteins and macromolecular assemblies for structural studies are increasing at a rapid rate. Structural biology will help to unravel the function of the newly discovered proteins and their molecular interactions within the cell and will ultimately provide the framework for developing new therapeutic drugs. Electron crystallography has become a valuable method for the structure determination of proteins and macromolecular complexes that are large or heterogeneous, difficult to obtain, unstable, insoluble, or that fail to yield crystals suitable for X-ray crystallographic analysis. Although electron microscopy (EM) and image analysis methods are rapidly improving and continuing to prove their value, advances in sample preparation have been limited, and general methods for obtaining ordered arrays of proteins for EM analysis are needed. In this thesis, I have described a method for promoting the formation of helical protein arrays. Helical crystallization on lipid nanotubes presents an alternative platform to explore fundamentals of protein ordering, intermolecular protein interaction and phase behavior. Three-dimensional (3-D) maps of wild-type and mutant streptavidin (SA) show that the molecular packing arrangements of SA on the surface of the lipid nanotubes differ from previously reported lattice packing of SA on biotinylated monolayers. His-tagged Perfringolysin O (PFO), a soluble toxin was used to demonstrate the utility of nickel-lipid nanotubes as a tool for structural studies of His-tagged proteins. Cryo-EM and image analysis of PFO helical arrays were used to reconstruct a 3-D density map. These studies have demonstrated that lipid nanotubes offer a robust and reproducible substrate for forming helical protein arrays which present a means for studying protein structure and structure-function relationships. Using EM and single-particle image analysis I calculated 2-D projection maps from images of oligomeric PFO prepore and pore complexes formed on lipid layers and identified an apparent conformational change from the prepore to the pore. In addition, I identified specific domains of the toxin molecule within the image and have thus determined the orientation of PFO monomers within the oligomeric complex.
Book Synopsis Electron Microscopy in Microbiology by :
Download or read book Electron Microscopy in Microbiology written by and published by Academic Press. This book was released on 1988-10-01 with total page 443 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and macromolecular levels. Many of the recent findings on ultrastructural features of microorganisms have been obtained with newly developed methods, though classical approaches have not lost their validity. Therefore, both conventional and new methods have been incorporated into this volume. The topics dealt with are meaningful not only in bacterial cytology but also in physiology, enzymology, biochemistry, and molecular biology, and include aspects of medical and biotechnological application.
Book Synopsis Unconventional Electron Microscopy for Molecular Structure Determination by : W. Hoppe
Download or read book Unconventional Electron Microscopy for Molecular Structure Determination written by W. Hoppe and published by Vieweg+Teubner Verlag. This book was released on 1979-01-01 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Conformational Dynamics by : Ke-li Han
Download or read book Protein Conformational Dynamics written by Ke-li Han and published by Springer Science & Business Media. This book was released on 2014-01-20 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.
