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Trends In Quantitative Enzyme Histochemistry
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Book Synopsis Trends in Enzyme Histochemistry and Cytochemistry by : David Evered
Download or read book Trends in Enzyme Histochemistry and Cytochemistry written by David Evered and published by John Wiley & Sons. This book was released on 2009-09-16 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Book Synopsis Enzyme Histochemistry by : Cornelis J. F. van Noorden
Download or read book Enzyme Histochemistry written by Cornelis J. F. van Noorden and published by Oxford University Press, USA. This book was released on 1992 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: The functional analysis of macromolecular structures in tissues and cells has been greatly enhanced by advances in histochemistry and cytochemistry. Enzyme histochemistry is becoming particularly important as new methods succeed in demonstrating and quantifying the activity of key regulatory enzymes. The specificity, precision, and reproducibility of enzyme histochemical methods are constantly improving. This practical laboratory handbook contains a selection of the most important enzyme histochemical techniques currently available for light microscopy. The methods included here were chosen because of their reliability and specificity, and all are clearly detailed in easy-to-follow protocols. The introductory chapter provides a good theoretical background to enzyme histochemistry, and the book will be of interest to all researchers in cell biology, pathology, biochemistry, and cell physiology.
Download or read book Enzyme Histochemistry written by Z. Lojda and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.
Book Synopsis Theory and Strategy in Histochemistry by : Hans Lyon
Download or read book Theory and Strategy in Histochemistry written by Hans Lyon and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: If you want practical information on how to use this book please refer to "Note to the Readers" p. VII. Histochemistry and cytochemistry are essential tools in biomedical research and routine service laboratories. Most texts on histochemistry fall into one of two categories: 1. Encyclopaedic texts covering all or nearly all information available on the whole or selected parts of histochemistry. 2. Reviews or surveys of methods found to be useful by the author(s). While the former category often appeals to the more philosophically inclined reader, direct guidance on the selection of technique may be difficult to find. In contrast, the latter category are often excellent sources for details on how to perform a particular method with a reasonable chance of success. Consideration of the exact mechanism of staining, of possible reasons for failure, and of alternative techniques are, however, frequently lacking. This book is an introduction to the scientific basis of histochemistry and is intended to provide a background for the selection and development of appro priate methods. It is not a "cook book" and readers expecting exhaustive methodological descriptions will be disappointed. Although most ofthe contributors to this book would not describe themselves as histochemists, they have all at some time found it essential to develop a basic understanding of histochemistry. This book contains the information they would have greatly appreciated ready access to at that time.
Author :Francisco Gonzalez-Lima Publisher :Springer Science & Business Media ISBN 13 :9780306460241 Total Pages :316 pages Book Rating :4.4/5 (62 download)
Book Synopsis Cytochrome Oxidase in Neuronal Metabolism and Alzheimer’s Disease by : Francisco Gonzalez-Lima
Download or read book Cytochrome Oxidase in Neuronal Metabolism and Alzheimer’s Disease written by Francisco Gonzalez-Lima and published by Springer Science & Business Media. This book was released on 1998-09-30 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a satellite to the 27th meeting of the Society for Neuroscience held October 28 in New Orleans, this symposium was dedicated to Dr. Margaret T.T. Wong-Reiley, developer of the method of cytochrome oxidase histochemical mapping of metabolism in the brain, on the 20th anniversary of this breakthrough. In this first ever compilation of papers applying this technique to the study of Alzheimer's, the 22 contributors--including Dr. Wong-Reiley--overview the role of cytochrome oxidase in cellular energy metabolism and neuronal activity and the methodology for its study via specific application examples. Part II treats the hypothesis that cytochrome inhibition may predict potential neurodegeneration, through discussions of animal models and genetic vulnerability of regions of the brain. Annotation copyrighted by Book News, Inc., Portland, OR
Download or read book The Histochemical Journal written by and published by . This book was released on 1985 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Histochemistry written by P. J. Stoward and published by John Wiley & Sons. This book was released on 1981 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Histochemistry by : Richard W. Horobin
Download or read book Histochemistry written by Richard W. Horobin and published by Elsevier. This book was released on 2014-05-16 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.
Book Synopsis Histochemical and Immunohistochemical Techniques by : Peter H. Bach
Download or read book Histochemical and Immunohistochemical Techniques written by Peter H. Bach and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mononuclear Phagocytes by : R. van Furth
Download or read book Mononuclear Phagocytes written by R. van Furth and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 792 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sixteen years have passed since the first Leiden Conference on Mononuclear Phagocytes. That first meeting led to new nomenclature, i. e. , the Mononuclear Phagocytes System (MPS), which was published in the Bulletin of the World Health Organization (R. van Furth, et aI. , Bull. WHO, 1972, 46: 845-852). The paradigm of the MPS has the advantage of being much more consistent than the Reticuloendothelial System (RES) (L. Aschoff, Ergebn. Inn. Med. Kinderheilk. 1924, 26:1), because cells with similar characteristics can be classified in the same category. The essence ota new concept is, according to Popper, that it must be susceptible to falsification. This has been attempted several times for the MPS by those who adhere to the RES. However, in biology no one can be certain of truth. Any theory must be based on experimental observations and/or theoretical consider ations and both should be presented in such a way that others can try to refute it, then or later. One may have a theoretical or a pragmatic preference for a theory which at a given time is regarded as possibly truc, but later it may be shown to be false. In other words, what we present in this volume is regarded as true at this moment, but others may consider it as not true and try to disprove it. Controversies often stimulate new research.
Book Synopsis Progress in Histochemistry and Cytochemistry by :
Download or read book Progress in Histochemistry and Cytochemistry written by and published by . This book was released on 1991 with total page 822 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods of Enzymatic Analysis: Fundamentals by : Hans Ulrich Bergmeyer
Download or read book Methods of Enzymatic Analysis: Fundamentals written by Hans Ulrich Bergmeyer and published by . This book was released on 1983 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Experimental Hepatocarcinogenesis by : M.B. Roberfroid
Download or read book Experimental Hepatocarcinogenesis written by M.B. Roberfroid and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: The meeting on experimental hepatocarcinogenesis which took place in Spa, Belgium at the end of May 1987 was the Second European Meeting. About 100 scientists, mostly from Europe but also from the United States, met there for three days in a very friendly atmosphere to exchange knowledge and ideas on experimental and human liver carcinogenesis. The main topics discussed during the meeting included general reviews on hepatocarcinogenesis, experimental models of hepa tocarcinogenesis, biology of hepatocarcinogenesis, and in vitro studies in hepatocarcinogenesis. They are all covered by the various chapters of this proceedings volume, which reflects the present state of knowledge in this important field of cancer research. The final aim of that research is to understand the basic mechanisms of carcinogenesis. The liver offers a parti cularly interesting tool to reach such a goal. Indeed, its biochemistry, its morphology, and its physiology are very diverse, but relatively well known. Various protocols have been developed to produce hepatocellular carcinomas or other malignant tumors. Their appearance is most often preceded by phenotypically altered foci and nodules which have been isolated and characterized. The major cell populations of normal, neoplastic, and malignant livers have been cultivated.
Book Synopsis Fluorescence Microscopy by : F. W. D. Rost
Download or read book Fluorescence Microscopy written by F. W. D. Rost and published by Cambridge University Press. This book was released on 1992 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.
Book Synopsis Quantitative and Qualitative Microscopy by : P. Michael Conn
Download or read book Quantitative and Qualitative Microscopy written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.
Author :Francisco Gonzalez-Lima Publisher :Springer Science & Business Media ISBN 13 :1475799365 Total Pages :294 pages Book Rating :4.4/5 (757 download)
Book Synopsis Cytochrome Oxidase in Neuronal Metabolism and Alzheimer’s Disease by : Francisco Gonzalez-Lima
Download or read book Cytochrome Oxidase in Neuronal Metabolism and Alzheimer’s Disease written by Francisco Gonzalez-Lima and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on an international symposium titled "Cytochrome oxidase in energy metabolism and Alzheimer's disease," held as a satellite to the 27th meeting of the Society for Neuroscience, New Orleans, 1997. The symposium was dedicated in honor of Dr. Margaret T. T. Wong-Riley because, in our opinion, the cytochrome oxidase histo chemical method introduced by Dr. Wong-Riley in 1979 was the most significant break through to map energy metabolism in the entire brain since the 2-deoxyglucose method introduced by Dr. Louis Sokoloff and colleagues in 1977. Both of these metabolic map ping techniques have made monumental contributions to brain research by allowing an integral view of brain activity. They have also developed into various specialized tech niques, including applications to the human brain. One of these new applications, which is described in detail in this book, is the quantitative cytochrome oxidase cytochemical method used to study Alzheimer's disease. The objective of this book is to describe the role of cytochrome oxidase in neuronal metabolism and Alzheimer's disease. Whether genetic or environmental, the pathogenesis of Alzheimer's disease involves a cascade of multiple intracellular events, eventually re sulting in failure of oxidative energy metabolism. Could impairment of cytochrome oxi dase in energy metabolism initiate the degenerative process? Cytochrome oxidase function and dysfunction are discussed in relationship to neuronal energy metabolism, neurodegen eration, and Alzheimer's disease. The book is made up of 10 chapters, divided into three major parts.
