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The Microbial Degradation Of Tce Trichloroethylene
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Book Synopsis The Microbial Degradation of TCE (Trichloroethylene). by : J. H. Wolfram
Download or read book The Microbial Degradation of TCE (Trichloroethylene). written by J. H. Wolfram and published by . This book was released on 1987 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this research was to characterize naturally occurring microorganisms capable of degrading trichloroethylene (TCE). Microorganisms tolerant to TCE were isolated from an aeration tower at Wurtsmith AFB MI and from industrial sludges at Hill AFB UT. Isolates from the Wurtsmith samples exhibited the highest density of cells with TCE as the only apparent organic carbon source and were selected as the inoculum for degradation studies. However, the capability of selected microorganisms to degrade TCE was not substantiated in both gas chromatography analyses and samples inoculated in culture bottles. Experiments using radiolabeled carbon-14 to study assimilation suggest that TCE is metabolized into a polar form by the microorganism. Subsequent cultures grown in radiolabeled TCE did not duplicate the previous results possible because of contamination, selection of variants or loss of culture. The organism responsible or the apparent TCE degradation may belong in the genus Citrobacter.
Book Synopsis Microbial Degradation of Trichloroethylene and Toluene by : François A. Roch
Download or read book Microbial Degradation of Trichloroethylene and Toluene written by François A. Roch and published by . This book was released on 1995 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Comparative Plant Uptake and Microbial Degradation of Trichloroethylene in the Rhizospheres of Five Plant Species-- Implications for Bioremediation of Contaminated Surface Soils by :
Download or read book Comparative Plant Uptake and Microbial Degradation of Trichloroethylene in the Rhizospheres of Five Plant Species-- Implications for Bioremediation of Contaminated Surface Soils written by and published by . This book was released on 1992 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this study was to collect data that would provide a foundation for the concept of using vegetation to enhance in situ bioremediation of contaminated surface soils. Soil and vegetation (Lespedeza cuneata, Paspalum notatum, Pinus taeda, and Solidago sp.) samples from the Miscellaneous Chemicals Basin (MCB) at the Savannah River Site were used in tests to identify critical plant and microbiological variables affecting the fate of trichloroethylene (TCE) in the root zone. Microbiological assays including phospholipid acid analyses, and 14C-acetate incorporation were conducted to elucidate differences in rhizosphere and nonvegetated soil microbial communities from the MCB. The microbial activity, biomass, and degradation of TCE in rhizosphere soils were significantly greater than corresponding nonvegetated soils. Vegetation had a positive effect on microbial degradation of 14C-TCE in whole-plant experiments. Soils from the MCB containing Lespedeza cuneata, Pinus taeda, and Glycine max mineralized greater than 25% of the 14C- TCE added compared with less than 20% in nonvegetated soils. Collectively, these results provide evidence for the positive role of vegetation in enhancing biodegradation.
Book Synopsis The Effects of Trichloroethylene (TCE) and Toluene on Soil Microbial Community Structure and Function by : Mark Edmund Fuller
Download or read book The Effects of Trichloroethylene (TCE) and Toluene on Soil Microbial Community Structure and Function written by Mark Edmund Fuller and published by . This book was released on 1995 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microbial Degradation of Volatile Anthropogenic Organic Chemicals by : Martin Alexander
Download or read book Microbial Degradation of Volatile Anthropogenic Organic Chemicals written by Martin Alexander and published by . This book was released on 1995 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis In Situ Bioremediation of Trichloroethylene Using Burkholderia Cepacia G4 PR1 by : Richard A. Snyder
Download or read book In Situ Bioremediation of Trichloroethylene Using Burkholderia Cepacia G4 PR1 written by Richard A. Snyder and published by . This book was released on 1998 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Effect of Acclimation on Biodegradation of Trichloroethylene and Toluene by Soil Microbial Populations by : Der-Yi Mu
Download or read book Effect of Acclimation on Biodegradation of Trichloroethylene and Toluene by Soil Microbial Populations written by Der-Yi Mu and published by . This book was released on 1993 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Comparative Plant Uptake and Microbial Degradation of Trichloroethylene in the Rhizospheres of Five Plant Species by : Todd Alan Anderson
Download or read book Comparative Plant Uptake and Microbial Degradation of Trichloroethylene in the Rhizospheres of Five Plant Species written by Todd Alan Anderson and published by . This book was released on 1994 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Microbial Community Structure in a Trichloroethylene Contaminated Aquifer During Toluene Stimulated Bioremediation by : Elica Monique Moss
Download or read book Microbial Community Structure in a Trichloroethylene Contaminated Aquifer During Toluene Stimulated Bioremediation written by Elica Monique Moss and published by . This book was released on 2004 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Enhanced Trichloroethylene Degradation Using Genetically-Engineered Microorganisms by :
Download or read book Enhanced Trichloroethylene Degradation Using Genetically-Engineered Microorganisms written by and published by . This book was released on 1996 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt: Novel recombinant TCE-degrading bacteria were created using the best known enzyme for TCE degradation, soluble methane monooxygenase(sMMO) of the soil bacterium Methylosinus trichosporium OB3b. sMMO degrades a wide range of halogenated hydrocarbons (HCFCs, chloroform, dichloroethane, etc.), and it degrades TCE 100 times faster than any other microbial enzyme. The mmo genes were cloned and expressed in Pseudomonas putida Fl, Agrobacterium tumefaciens, and Rhizobium meliloti using plasmids pSMMO20 and pSMMO40 created by the Wood laboratory. In addition, a novel fixed-film bioreactor has been constructed and optimized to mineralize TCE in the gas phase.
Book Synopsis The Influence of Soil Organic Matter on the Fate of Trichloroethylene in Soil by :
Download or read book The Influence of Soil Organic Matter on the Fate of Trichloroethylene in Soil written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Trichloroethylene Degradation by Mixed Culture and Zero-valent Iron by : Kaili Qian
Download or read book Trichloroethylene Degradation by Mixed Culture and Zero-valent Iron written by Kaili Qian and published by . This book was released on 2010 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Multiple Co-Substrates for Biostimulation of TCE Degradation by :
Download or read book Multiple Co-Substrates for Biostimulation of TCE Degradation written by and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this Phase II SBIR project was to test and demonstrate the feasibility of using multiple co-substrates to stimulate in situ biodegradation of volatile organochloride compounds (VOCs) including trichloroethylene (TCE), cis- 1,2 dichloroethylene (DCE) and vinyl chloride (VC). The project also evaluated the use of novel substrate delivery and groundwater capture systems for use during in situ biostimulation applications with gaseous cosubstrates. The work involved a field-scale demonstration of propane and oxygen injection to stimulate microbial degradation of VOCs in an aquifer underlying the Lakehurst, NJ Naval Air Engineering Station (NAES).
Book Synopsis Bioremediation and Biotechnology by : Khalid Rehman Hakeem
Download or read book Bioremediation and Biotechnology written by Khalid Rehman Hakeem and published by Springer Nature. This book was released on 2020-01-26 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: Toxic substances threatens aquatic and terrestrial ecosystems and ultimately human health. The book is a thoughtful effort in bringing forth the role of biotechnology for bioremediation and restoration of the ecosystems degraded by toxic and heavy metal pollution. The introductory chapters of the book deal with the understanding of the issues concerned with the pollution caused by toxic elements and heavy metals and their impacts on the different ecosystems followed by the techniques involved in monitoring of the pollution. These techniques include use of bio-indicators as well as modern techniques for the assessment and monitoring of toxicants in the environment. Detailed chapters discussing the role of microbial biota, aquatic plants, terrestrial plants to enhance the accumulation efficiency of these toxic and heavy metals are followed by remediation techniques involving myco-remediation, bio-pesticides, bio-fertilizers, phyto-remediation and rhizo-filtration. A sizable portion of the book has been dedicated to the advanced bio-remediation techniques which are finding their way from the laboratory to the field for revival of the degraded ecosystems. These involve bio-films, micro-algae, genetically modified plants and filter feeders. Furthermore, the book is a detailed comprehensive account for the treatment technologies from unsustainable to sustainable. We believe academicians, researchers and students will find this book informative as a complete reference for biotechnological intervention for sustainable treatment of pollution.
Book Synopsis Trichloroethylene Contaminated Soil Clean-up Using Surfactant-based Separation Technology and Bioremediation by : Sasikarn Chuahom
Download or read book Trichloroethylene Contaminated Soil Clean-up Using Surfactant-based Separation Technology and Bioremediation written by Sasikarn Chuahom and published by . This book was released on 2006 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surfactant-based separation technology and bioremediation was integrated to enhance the removal of trichloroethylene (TCE) from soil. In this system, cloud point extraction by non-ionic surfactant was conducted to separate high amount of TCE into the surfactant-rich phase and then bioremediation was integrated into the system by adding bacteria to co-metabolize the remaining TCE. Six surfactant systems including SURFONIC TDA-5, SURFONIC TDA-6, SURFONIC L24-7, NEODOL 91-5, NEODOL 91-6 without electrolyte addition and DTAB/DOWFAX (2:1 molar ratio) with 0.8 M NaCI were studied. These surfactants induced a phase separation with the surfactant-rich phase presented on top of the solution thus preventing the accumulation of surfactant on soil particles. The results found that SURFONIC TDA-6, SURFONIC L24-7, an NEODOL 91-6 did not inhibit TCE degradability of either Rhodococcus sp. L4 or Phodococcus sp. P3 bacteria while others killed the bacteria. Rhodococcus sp. L4 degraded TCE effectively in the presence of SURFONIC TDA-6, in which more than 58% of 10 ppm TCE was reduced within 24 hours compared to only 30% of TCE removal in control treatment (without the bacteria), SURFONIC TDA-6 was then selected for determining the optimal condition for TCE extraction consisting of equilibrium time, contact time between surfactant solution and soil and initial concentration of surfactant. The optimal condition for TCE extraction by cloud point technique were as followed; 72 hours of equilibrium time, 1 lour of contact time between soil and surfactant solution, and 70 mM of initial concentration of surfactant. These acquired conditions were later applied to compare the effectiveness of three TCE treatment processes including: (1) bioremediation, (2) surfactant extraction and (3) integrated technique. TCE removal efficiency was determined from the remaining TCE concentration in soil after treatment. The TCE removal efficiency of 100 ppm TCE contaminated soil by bioremediation, surfactant extraction and integrated technique were about 74%, 74% and 94%, respectively. Morever, TCE was mineralized as showed by the increase of chloride ions after remediation by bioremediation and integrated technique. When increased the amount of initial TCE to 300 ppm, the removal efficiency of the integrated technique was about 94% which was around 30% higher than either technique alone. The result found that soil remediation by the integrated technique had the highest TCE removal efficiency.
Book Synopsis Sequential Anaerobic-Aerobic Biodegradation of Trichloroethylene and 1,4-Dioxane by : Alexandra LaPat Polasko
Download or read book Sequential Anaerobic-Aerobic Biodegradation of Trichloroethylene and 1,4-Dioxane written by Alexandra LaPat Polasko and published by . This book was released on 2017 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chlorinated ethenes, such as trichloroethylene (TCE) and their stabilizers, such as 1,4- dioxane, are widespread groundwater contaminants. Bioremediation can be an effective approach, but opposing redox conditions favored by chlorinated ethane-and 1,4-dioxane- degrading bacteria pose a challenge for their concurrent bioremediation. We engineered a microbial community composed of the anaerobic chlorinated ethene-degrading consortium (KB-1i ) and aerobic (Pseudonocardia dioxanivorans CB1190 (CB1190)) bacterial strain, which uses 1,4-dioxane. After anaerobic incubation and TCE reduction, CB1190 + KB-1i coculture was viable and rapidly biodegraded 1,4-dioxane in the presence of oxygen. Aerobic biodegradation of cis-1,2-dichloroethylene (cDCE) by CB1190 was also described. As a plume disperses downgradient, the redox conditions change from anaerobic (source zone) to aerobic (leading edge). The results from this study demonstrate that the engineered microbial community can survive redox changes and biodegrade chlorinated ethenes and 1,4-dioxane. This approach could reduce the cost, energy, and substrates required for in-situ bioremediation of contaminant mixtures.
Book Synopsis Aerobic Biodegradation of Trichloroethylene by : M. J. K. Nelson
Download or read book Aerobic Biodegradation of Trichloroethylene written by M. J. K. Nelson and published by . This book was released on 1987 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: Samples, suspected of having a capability to biologically transform trichloroethylene (TCE), were provided by Tyndall Air Force Base for verification and characterization of activity. Biological transformation of TCE was not observed in these samples. Other soil and water samples, obtained from the Pensacola area, were therefore screened for TCE degradation activity. One sample was found to be responsible for the metabolic activity was isolated. The isolated organism degrades TCE (up to 3.4 micro mole) to less than 0.02 micro mole within 24 hours. TCE degradation occurred only when water from the original site of isolation and 02 were in the medium. The isolate converted TCE into CO2 and unidentified nonvolatile products. Phenol, toluene ortho- and meta-cresol were found to replace the site water requirement for TCE metabolism.
