Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
The Determination Of Glycopeptides And Glycoproteins By Liquid Chromatography Mass Spectrometry And Tandem Mass Spectrometry
Download The Determination Of Glycopeptides And Glycoproteins By Liquid Chromatography Mass Spectrometry And Tandem Mass Spectrometry full books in PDF, epub, and Kindle. Read online The Determination Of Glycopeptides And Glycoproteins By Liquid Chromatography Mass Spectrometry And Tandem Mass Spectrometry ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis The Determination of Glycopeptides and Glycoproteins by Liquid Chromatography/mass Spectrometry and Tandem Mass Spectrometry by : James Joseph Conboy
Download or read book The Determination of Glycopeptides and Glycoproteins by Liquid Chromatography/mass Spectrometry and Tandem Mass Spectrometry written by James Joseph Conboy and published by . This book was released on 1992 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mass Spectrometry–Based Glycoproteomics and Its Clinic Application by : Haojie Lu
Download or read book Mass Spectrometry–Based Glycoproteomics and Its Clinic Application written by Haojie Lu and published by CRC Press. This book was released on 2021-09-20 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: As one of the most extensive and important protein post-translational modifications, glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently, researchers have focused on the need to characterize protein glycosylation sites, structures, and their degree of modification, to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative, quantitative, and data analysis, together with their clinical applications. Significant examples are discussed to illustrate the principles, laboratory protocols, and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine. Editor: Haojie Lu is a professor at Fudan University, specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.
Book Synopsis Mass Spectrometry of Glycoproteins by : Arnaud Delobel
Download or read book Mass Spectrometry of Glycoproteins written by Arnaud Delobel and published by Humana. This book was released on 2021-07-04 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
Book Synopsis Mass Spectrometry: Modified Proteins and Glycoconjugates by : A.L. Burlingame
Download or read book Mass Spectrometry: Modified Proteins and Glycoconjugates written by A.L. Burlingame and published by Gulf Professional Publishing. This book was released on 2005-12-13 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines
Book Synopsis Glycopeptide Detection, Selection, and Characerization Through a Liquid Chromatography-mass Spectrometry-differential Ion Mobility Platform by : Daniel Graham Delafield
Download or read book Glycopeptide Detection, Selection, and Characerization Through a Liquid Chromatography-mass Spectrometry-differential Ion Mobility Platform written by Daniel Graham Delafield and published by . This book was released on 2018 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Glycopeptides and Related Compounds by : David G. Large
Download or read book Glycopeptides and Related Compounds written by David G. Large and published by CRC Press. This book was released on 1997-05-06 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Presents state-of-the-art methods for the synthesis, analysis, and conformational investigation of glycoproteins and glycopeptides. Discusses the history of glycopeptide synthesis, therapeutic applications, and the future of research."
Book Synopsis Trace Determination of Glycoproteins by Mass Spectrometry Coupled to High Performance Liquid Chromatography and Capillary Electrophoresis by : Chuanliang Liu
Download or read book Trace Determination of Glycoproteins by Mass Spectrometry Coupled to High Performance Liquid Chromatography and Capillary Electrophoresis written by Chuanliang Liu and published by . This book was released on 1995 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao
Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.
Book Synopsis Combination of Nonspecific Proteolysis, Tandem Mass Spectrometry and Bioinformatics for Extensive Site-specific Glycosylation Analysis in Protein Mixtures by : Charles Chuks Nwosu
Download or read book Combination of Nonspecific Proteolysis, Tandem Mass Spectrometry and Bioinformatics for Extensive Site-specific Glycosylation Analysis in Protein Mixtures written by Charles Chuks Nwosu and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are a unique class of biological molecules known to perform a wide range of important functions. Amongst the numerous post-translational modifications (PTMs) that decorate proteins, glycosylation is one of the most common and diverse affecting about 70% of human proteins. Glycosylation is known to influence proper protein folding, increase protein stability and enhance cell-cell adhesion. These and many other functions make glycoproteins an important and interesting analyte to study. Protein glycosylation has been largely ignored in numerous traditional proteomic and glycomic experiments. This is partly due to the diverse and complex manner by which glycans particularly decorate proteins and the lack of effective analytical techniques available for comprehensively characterizing protein glycosylation. This dissertation addresses the current limitations of protein glycosylation analysis via strictly proteomic and glycomic strategies and it provides a solution. The effectiveness of the use of nonspecific proteolysis combined with mass spectrometric analysis towards achieving site-specific glycosylation in single purified proteins and in complex protein mixtures is described. Chapter one provides an introduction to glycoproteomics including a summary of common methods used to investigate protein glycosylation. Chapter one concludes with an overview of mass spectrometry and recent advancements that have permitted its use for the characterization of protein glycosylation. Chapter two presents a method for the enhanced detection and identification of glycopeptides in negative ion mode mass spectrometry via matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron mass spectrometry (FT-ICR MS). Negative ion mode is not commonly used in MS analyses, but appears well suited for glycopeptides. An approach for the simultaneous and extensive site-specific N- and O-glycosylation analysis in a simple protein mixture is presented in chapter three. Here, immobilized pronase is shown to significantly reduce a mixture of N- and O-glycoproteins to glycopeptides, which are then investigated by nano-flow liquid chromatography coupled with a quadrupole time-of-flight mass spectrometer (nano-LC/Q-TOF MS) and identified with an in-house data analysis software. This approach is shown to provide glycopeptide footprints that identify sites of protein glycosylation in a robust and sensitive manner. A targeted investigation of N- and O-glycosylation in complex protein mixtures via novel in-gel nonspecific proteolysis and analysis of the resulting N- and O-glycopeptides by nano-LC-MS/MS is described in chapter four. Finally, chapter five details a glycomic study involving the comparison of the human and bovine milk N-glycome via peptide N-glycosidase F (PNGase F) digestion and nano-LC-MS/MS analysis of the purified N-glycans.
Book Synopsis Analysis of Protein Post-Translational Modifications by Mass Spectrometry by : John R. Griffiths
Download or read book Analysis of Protein Post-Translational Modifications by Mass Spectrometry written by John R. Griffiths and published by John Wiley & Sons. This book was released on 2016-10-12 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers all major modifications, including phosphorylation, glycosylation, acetylation, ubiquitination, sulfonation and and glycation Discussion of the chemistry behind each modification, along with key methods and references Contributions from some of the leading researchers in the field A valuable reference source for all laboratories undertaking proteomics, mass spectrometry and post-translational modification research
Download or read book Glycomics written by Nicolle H. Packer and published by Humana Press. This book was released on 2009-07-16 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Book Synopsis High-Throughput Glycomics and Glycoproteomics by : Gordan Lauc
Download or read book High-Throughput Glycomics and Glycoproteomics written by Gordan Lauc and published by Humana Press. This book was released on 2018-06-27 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.
Book Synopsis Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry by : Ganglong Yang
Download or read book Protein Glycosylation – Advances in Identification, Characterization and Biological Function Elucidation using Mass Spectrometry written by Ganglong Yang and published by Frontiers Media SA. This book was released on 2022-03-14 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Essentials of Glycobiology by : Ajit Varki
Download or read book Essentials of Glycobiology written by Ajit Varki and published by CSHL Press. This book was released on 1999 with total page 694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Book Synopsis Differential Ion Mobility Spectrometry by : Alexandre A. Shvartsburg
Download or read book Differential Ion Mobility Spectrometry written by Alexandre A. Shvartsburg and published by CRC Press. This book was released on 2008-12-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.
Book Synopsis Methods On Glycoconjugates by : André Verbert
Download or read book Methods On Glycoconjugates written by André Verbert and published by CRC Press. This book was released on 1995-07-01 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: By guiding the reader toward answering basic questions on the structural analysis of glycoconjugates using conventional techniques, this book studies methods such as chemical cleavage, molar composition, use of enzymes, use of lectins and typical fractionation procedures such as HPLC, GLC, TLC, and other chromatographic systems. More sophisticated techniques such as NMR or MS are described to give the reader the essential information needed to adapt procedures to his/her requirements. This book will serve as a practical manual designed for scientists not familiar with glycoconjugate methodologies, but whose research work requires contact with analytical problems or metabolic investigations in glycobiology. It is the result of twenty years of experience of summer schools held in a laboratory environment and will acquaint the reader with widely used techniques in the area rather than cataloguing an exhaustive list of methods. This will help any biochemist to develop an experiment with ease.
Book Synopsis New Approaches for Quantitative Analysis of Glycopeptides Using Liquid Chromatography-mass Spectrometry by : Yining Huang
Download or read book New Approaches for Quantitative Analysis of Glycopeptides Using Liquid Chromatography-mass Spectrometry written by Yining Huang and published by . This book was released on 2016 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt:
