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The Analysis Of Limited Quantities Of Amino Acids And Peptides Using Capillary Electrophoresis And Laser Induced Fluorescence Detection
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Book Synopsis The Analysis of Limited Quantities of Amino Acids and Peptides Using Capillary Electrophoresis and Laser Induced Fluorescence Detection by : Beverly Nickerson
Download or read book The Analysis of Limited Quantities of Amino Acids and Peptides Using Capillary Electrophoresis and Laser Induced Fluorescence Detection written by Beverly Nickerson and published by . This book was released on 1991 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Tara Lee Massie
Download or read book Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Tara Lee Massie and published by . This book was released on 2010 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a versatile and sensitive analytical tool with many potential applications. Its advantages over other chromatographic methods include minimal solvent and sample requirements, low waste volumes, simple instrumentation, and diversity of analytes studied. CE is one of the most efficient separation techniques available for the analysis of both large and small molecules, but because few analytes are natively fluorescent, it is necessary to bind them with fluorescent tags if LIF detection is to be employed. These tags increase sensitivity and provide additional separation from components of the sample that do not bind the tags. In this dissertation, four applications of CE-LIF for the determination of tagged analytes are documented. First, a rapid CE-LIF method was developed for the determination of gamma-aminobutyric acid (GABA) and other important brain biogenic amines and amino acids present in biological samples at low levels. The high efficiency and sensitivity necessary to identify toxicologically relevant changes in neurotransmitter levels in brain tissue and microdialysates following Mn exposure was achieved, and the method required minimum sample work-up and quantities of sample for analysis. A linear relationship between the concentration of GABA and the CE-LIF peak height was established, with correlation coefficients of 0.989 or better for four neurotransmitters. Second, the use of two squarylium-based fluorescent dyes (bis-SQHN-4d and SQHN-3c) as noncovalent protein labels was investigated. The fluorescence emissions of both dyes were enhanced upon complexation with model proteins, demonstrating promise as noncovalent fluorescent protein labels. Third, the nature of interactions between two boronic acid-based probes (SQTM-BA1 and ANQW-BA1) and two model proteins (bovine serum albumin (BSA) and hemagglutinin (HA)) have been explored. The complex between SQTM-BA1 and BSA was the only complex to show increased fluorescence emission, and the role of electrostatic interactions between SQTM-BA1 and BSA in complex formation was explored. Lastly, CE-LIF methods were developed to label and quantify turnip yellow mosaic virus (TYMV). Noncovalent labels Red-1c and NN127 effectively labeled the surface proteins of the virus, and the labeled viruses were then analyzed by CE-LIF. The CE-LIF methods developed herein for the analysis of neurotransmitters, free solution proteins, and viruses demonstrate the versatility of the technique, especially when coupled with fluorescent labeling.
Book Synopsis Analysis of Peptides and Proteins by Electrophoretic Techniques by : Angela R. Piergiovanni
Download or read book Analysis of Peptides and Proteins by Electrophoretic Techniques written by Angela R. Piergiovanni and published by MDPI. This book was released on 2019-07-23 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Book Synopsis APPLICATION OF CAPILLARY ELECT by : 易秀麗
Download or read book APPLICATION OF CAPILLARY ELECT written by 易秀麗 and published by Open Dissertation Press. This book was released on 2017-01-27 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "The Application of Capillary Electrophoresis With Laser-induced Fluorescence Detection in Quantifying the Endogenous Amino Acid Pool of Mouse Embryos" by 易秀麗, Sau-lai, Yik, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3122383 Subjects: Capillary electrophoresis Fluorescence spectroscopy Amino acids Mice - Embryos
Book Synopsis Laser-based Ultra-trace Analysis in Liquid Chromatography by : Owe Orwar
Download or read book Laser-based Ultra-trace Analysis in Liquid Chromatography written by Owe Orwar and published by . This book was released on 1994 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Nanoscale Protein Analysis Utilizing Capillary Electrophoresis and Laser-induced Fluorescence Detection by : Michael D. Harvey
Download or read book Nanoscale Protein Analysis Utilizing Capillary Electrophoresis and Laser-induced Fluorescence Detection written by Michael D. Harvey and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The trend towards high throughput applications and miniaturization necessitates approaches capable of microlitre volume sampling and low protein concentration detection. Furthermore, one of the major trends in high throughput screening is the growing replacement of technologies that depend on radioactivity to generate a signal with those that rely on fluorescence. This trend towards non-radioactive detection in general can be understood by some of the advantages inherent to these methods over radioactive modes. These include a significant reduction in safety concerns leading to a relaxation of strict laboratory procedures, elimination of expensive waste disposal, extended shelf-life of labeled reagents, and the possibility of acquiring multiplexed data through the spectral isolation of different wavelength signals. A variety of capillary electrophoretic (CE) approaches utilizing laser-induced fluorescence (LIF) have thus been developed, providing researchers with valuable tools in protein analysis. Various covalent and non-covalent fluorescent derivatization approaches have been investigated, with emphasis on biochemical and/or clinical applications. The non-covalent dye, NanoOrange, is used as a clinical diagnostic tool for early disease diagnosis, quantitating nanomolar concentrations of human serum albumin in solution, and obtaining fluorescence-based biofluid profiles. An alternate non-covalent labeling approach utilizing the fluorescent probe, Sypro Red, and capillary gel electrophoresis allows for rapid, sensitive analysis of protein sample purity as well as molecular weight determination. These two non-covalent approaches are complemented by the development of a fluorescent Insulin-Like Growth Factor-I (IGF-I) analog for use in bioanalytical applications. Specific derivatization reaction conditions were developed to selectively label the N-terminus of the analog hence preserve biological activity. High-performance liquid chromatography and electrospray mass spectrometry were used to confirm the extent of labeling and modification site. Antibody recognition of this fluorescent analog was evaluated using CE-LIF, illustrating the clinical utility of this diagnostic reagent. In addition to the above CE-LIF approaches, a fourth capillary electrophoretic tool is provided for the clinical chemist. Rapid analysis of biofluids is of significant importance in early disease diagnosis. As such, an extensive CE-based analysis of human seminal plasma is presented. Separation conditions, sample stability, and protein/non-protein zone identification issues are addressed. This study and the CE-LIF methodologies discussed above represent original approaches to nanoscale protein analysis.
Book Synopsis Rapid Separation of Amino Acids Using Capillary Electrophoresis with Laser-induced Fluorescence by : Stephanie Ann Cunningham
Download or read book Rapid Separation of Amino Acids Using Capillary Electrophoresis with Laser-induced Fluorescence written by Stephanie Ann Cunningham and published by . This book was released on 2007 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis American Doctoral Dissertations by :
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1991 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides by : Guido Fridolin Verbeck
Download or read book Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides written by Guido Fridolin Verbeck and published by . This book was released on 1999 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by : Qifeng Xue
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Site-specific Fluorescent Derivatization of Peptides for Characterization by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Sara Jane Sudmeier
Download or read book Site-specific Fluorescent Derivatization of Peptides for Characterization by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Sara Jane Sudmeier and published by . This book was released on 1998 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Large Biomolecules by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Pertti Juha Viskari
Download or read book Analysis of Large Biomolecules by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Pertti Juha Viskari and published by . This book was released on 2003 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Biomolecules Using Interfacial Capillary Electrophoresis with Laser Induced Fluorescence Detection by : Lorna Alice Hunter Pehl
Download or read book Analysis of Biomolecules Using Interfacial Capillary Electrophoresis with Laser Induced Fluorescence Detection written by Lorna Alice Hunter Pehl and published by . This book was released on 2000 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Analysis of Proteins and Peptides Using Microcolumn Analytical Methods by : Edwin Louis Gump
Download or read book Analysis of Proteins and Peptides Using Microcolumn Analytical Methods written by Edwin Louis Gump and published by . This book was released on 1998 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Peptide Analysis by Capillary Electrophoresis Coupled with Laser Induced Fluorescence and Mass Spectrometry by : Roza Wojcik
Download or read book Advances in Peptide Analysis by Capillary Electrophoresis Coupled with Laser Induced Fluorescence and Mass Spectrometry written by Roza Wojcik and published by . This book was released on 2011 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection by : Sheri Joanne Lillard
Download or read book Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection written by Sheri Joanne Lillard and published by . This book was released on 1996 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Subattomole Amino Acid Analysis by Laser-Induced Fluorescence by : YF. Cheng
Download or read book Subattomole Amino Acid Analysis by Laser-Induced Fluorescence written by YF. Cheng and published by . This book was released on 1990 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subattomole analysis of fluorescein isothiocyanate (FITC) derivatives of amino acids is produced by combining capillary zone electrophoresis for high-efficiency separation with laser-induced fluorescence for high sensitivity detection. To optimize the separation process, several buffer systems were investigated. A pH 10 carbonate buffer provided good separation of 14 FITC amino acids. Concentration detection limits range from 5 x 10-12M for alanine to 9 x 10-11M for lysine, injected onto the column; ~9 x 10-21 mol of alanine is contained within the ~1-nL injection volume at the detection limit. The alanine detection limit corresponds to less than 6000 molecules injected onto the column and represents a four order of magnitude improvement in the state of the art for fluorescent detection of amino acids and a six order of magnitude improvement in the state of the art for the detection limit for isothiocyanate derivatives of amino acids.
