Técnicas de biología molecular II

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Author :
Publisher : Cambridge Stanford Books
ISBN 13 :
Total Pages : 484 pages
Book Rating : 4./5 ( download)

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Book Synopsis Técnicas de biología molecular II by : John Kaisermann

Download or read book Técnicas de biología molecular II written by John Kaisermann and published by Cambridge Stanford Books. This book was released on with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Desde alrededor de 1960, los biólogos moleculares han desarrollado métodos para identificar, aislar y manipular componentes moleculares en células que incluyen DNA, RNA y proteínas. Contenido de este libro: CRISPR edición de genes, CRISPR, Prime edición, Anti-CRISPR, Transfección, Gen knock-in, Gene knockout, GeneTalk, Haplarithm, Haplarithmisis, Helicase-dependent amplification, Immunoprecipitation, Enfoque isoeléctrico, Isopeptag, Jumping library, Knockout moss, Kodecyte, Kodevirion, Reacción en cadena de la ligasa, ligadura (biología molecular), asistida por imán transfection, MassTag-PCR, secuenciación de Maxam-Gilbert, métodos para investigar las interacciones proteína-proteína, materia oscura microbiana, Microsatellite enrichment, sistema de cultivo de perfusión Minusheet, MNase-seq, Resonancia de plasmones de superficie multiparamétrica, mutagénesis (técnica de biología molecular), Northern blot, Northwestern blot, ensayo de protección de nucleasa, determinación de estructura de ácido nucleico, restricción de oligómeros, oligotipado (secuenciación), oligotipado (taxonomía), cadena de polimerasa de extensión de solapamiento reacción, Paired-end tag, pBLU, pBR322, Peak calling, Perturb-seq, Etiquetado de fotoafinidad, mapeo físico, vector de transformación de plantas, placa hybridization, plásmido, plasmidoma, reacción en cadena de la polimerasa, PRIME (incorporación de PRobe mediada por enzimas), Promoter bashing, pUC19, centrifugación de velocidad de zona, amplificación de la polibrasa recombinante, Inverso northern blot, Inverso transfection, análisis de espaciador intergénico ribosómico, perfil Ribosome, dependiente de RNasa H PCR, transcripción de escorrentía, secuenciación Sanger, ensayo de selección y unión de amplificación, secuenciación de células individuales, Single- secuenciación de cadena de plantilla de célula DNA, transcriptómica de célula única, SMiLE-Seq, snRNA-seq, Sono-Seq, Southern blot, Southwestern blot, sondeo de isótopos estables, proceso de extensión escalonada, Strep-tag, Streptamer, Subcloning, inmunoensayo de fibra óptica envolvente, tecnología de matriz de suspensión, cultivo sincrónico, TA cloning, TBST, TCP-seq, Toeprinting assay, inferencia de trayectoria, microscopía electrónica de transmisión DNA secuenciación, Univec, VectorDB, ensayo de viabilidad, ViroCap, Western blot, Western blot normalización

Sperm Differentiation and Spermatozoa Function: Mechanisms, Diagnostics, and Treatment - Volume II

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Publisher : Frontiers Media SA
ISBN 13 : 2889766098
Total Pages : 300 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Sperm Differentiation and Spermatozoa Function: Mechanisms, Diagnostics, and Treatment - Volume II by : Tomer Avidor-Reiss

Download or read book Sperm Differentiation and Spermatozoa Function: Mechanisms, Diagnostics, and Treatment - Volume II written by Tomer Avidor-Reiss and published by Frontiers Media SA. This book was released on 2022-07-26 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Biology of the Cell

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Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Networking in Brucellosis Research II

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Author :
Publisher : United Nations University Press
ISBN 13 : 9789280810103
Total Pages : 232 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Networking in Brucellosis Research II by :

Download or read book Networking in Brucellosis Research II written by and published by United Nations University Press. This book was released on with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:

TRASTORNOS BIPOLARES,

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 9788407001738
Total Pages : 564 pages
Book Rating : 4.0/5 (17 download)

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Book Synopsis TRASTORNOS BIPOLARES, by : E. VIETA

Download or read book TRASTORNOS BIPOLARES, written by E. VIETA and published by Springer Science & Business Media. This book was released on 2000-12-19 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt:

II Meeting of the Interamerican Commission on Animal Health, Brasilia, D.F., Brazil 1985, April 29-May 1st., 1985

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Author :
Publisher : IICA
ISBN 13 : 9789290390916
Total Pages : 330 pages
Book Rating : 4.3/5 (99 download)

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Book Synopsis II Meeting of the Interamerican Commission on Animal Health, Brasilia, D.F., Brazil 1985, April 29-May 1st., 1985 by : Interamerican Commission on Animal Health. Meeting

Download or read book II Meeting of the Interamerican Commission on Animal Health, Brasilia, D.F., Brazil 1985, April 29-May 1st., 1985 written by Interamerican Commission on Animal Health. Meeting and published by IICA. This book was released on 1985 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Auditory Efferent System: New Insights from Cortex to Cochlea

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Publisher : Frontiers Media SA
ISBN 13 : 2889199355
Total Pages : 122 pages
Book Rating : 4.8/5 (891 download)

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Book Synopsis Auditory Efferent System: New Insights from Cortex to Cochlea by : Paul H. Delano

Download or read book Auditory Efferent System: New Insights from Cortex to Cochlea written by Paul H. Delano and published by Frontiers Media SA. This book was released on 2016-09-06 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main function of the sensory systems is the transducing of external stimuli into bioelectrical signals, which are conducted through afferent pathways from sensory epithelia to the brain. However, it is known that descending projections are ubiquitous in the different sensory modalities, and in the case of auditory efferents connect the cerebral cortex with sensory receptor cells. Several functions have been attributed to the efferent system, including protection to acoustic trauma, unmasking of auditory stimuli in background noise, balance of interaural sensitivity and some cognitive functions like modulation of cochlear sensitivity during selective attention to auditory or visual stimuli. In addition there is evidence of a possible involvement of the efferent system in the etiology or treatment of some clinical pathologies like tinnitus. In this e-book, entitled “Auditory Efferent System: New Insights from Cortex to Cochlea”, we aimed to give an overview of the advances concerning the descending projections from the auditory cortex to subcortical nuclei and the olivocochlear system. In addition, different theoretical proposals of efferent functions are presented. We think that this e-book is an important contribution to the understanding of the efferent system in mammals, merging auditory-cortex literature with studies performed in the olivocochlear system.

Infecciones respiratorias en UCI

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Publisher : Springer Science & Business Media
ISBN 13 : 9788407001875
Total Pages : 330 pages
Book Rating : 4.0/5 (18 download)

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Book Synopsis Infecciones respiratorias en UCI by : Antoni Torres

Download or read book Infecciones respiratorias en UCI written by Antoni Torres and published by Springer Science & Business Media. This book was released on 2000-12-20 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant-Pest Interactions, Volumes I, II and III

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Author :
Publisher : Frontiers Media SA
ISBN 13 : 2889742873
Total Pages : 272 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis Plant-Pest Interactions, Volumes I, II and III by : Isabel Diaz

Download or read book Plant-Pest Interactions, Volumes I, II and III written by Isabel Diaz and published by Frontiers Media SA. This book was released on 2022-03-09 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook comprises Volume I, Volume II and Volume III of the Research Topic Plant-Pest Interactions. Topic Editors Colette Broekgaarden and Martin De Vos are employed by KeyGene N.V.. All other Topic Editors declare no competing interests with regards to the Research Topic subject.

Enciclopedia de Lingüística Hispánica

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Publisher : Routledge
ISBN 13 : 1317498011
Total Pages : 2157 pages
Book Rating : 4.3/5 (174 download)

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Book Synopsis Enciclopedia de Lingüística Hispánica by : Javier Gutiérrez-Rexach

Download or read book Enciclopedia de Lingüística Hispánica written by Javier Gutiérrez-Rexach and published by Routledge. This book was released on 2016-01-29 with total page 2157 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Enciclopedia de Linguistica Hispánica provides comprehensive coverage of the major and subsidiary fields of Spanish linguistics. Entries are extensively cross-referenced and arranged alphabetically within three main sections: Part 1 covers linguistic disciplines, approaches and methodologies. Part 2 brings together the grammar of Spanish, including subsections on phonology, morphology, syntax and semantics. Part 3 brings together the historical, social and geographical factors in the evolution of Spanish. Drawing on the expertise of a wide range of contributors from across the Spanish-speaking world the Enciclopedia de Linguistica Hispánica is an indispensable reference for undergraduate and postgraduate students of Spanish, and for anyone with an academic or professional interest in the Spanish language/Spanish linguistics.

Rotifera VIII: A Comparative Approach

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Publisher : Springer Science & Business Media
ISBN 13 : 9401147825
Total Pages : 487 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Rotifera VIII: A Comparative Approach by : E. Wurdak

Download or read book Rotifera VIII: A Comparative Approach written by E. Wurdak and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rotifera VIII: A Comparative Approach is a record of the proceedings of the VIIIth International Rotifer Symposium which was held in Collegeville, Minnesota, USA, on June 22-27, 1997. It contains review papers and reports of recent research findings along with the presentation of new methods in rotifer biology. The publications contained in this volume reflect the wide diversity of approaches, methods of analysis and conclusions that characterize research on the Rotifera. Some of the topics addressed are: rotifer distribution, responses to biotic and abiotic factors, genetic profile of individuals and populations, rotifer feeding and mating behavior, morphology, phylogeny and taxonomy. These studies will be of great interest to invertebrate zoologists and limnologists, particularly those interested in freshwater habitats.

Recombinant protein expression in microbial systems

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Publisher : Frontiers E-books
ISBN 13 : 2889192946
Total Pages : 103 pages
Book Rating : 4.8/5 (891 download)

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Book Synopsis Recombinant protein expression in microbial systems by : Eduardo A. Ceccarelli

Download or read book Recombinant protein expression in microbial systems written by Eduardo A. Ceccarelli and published by Frontiers E-books. This book was released on 2014-10-02 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.

Transcription Regulation - Brain Development and Homeostasis - A Finely Tuned and Orchestrated Scenario in Physiology and Pathology, Volume II

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Publisher : Frontiers Media SA
ISBN 13 : 2832533817
Total Pages : 169 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Transcription Regulation - Brain Development and Homeostasis - A Finely Tuned and Orchestrated Scenario in Physiology and Pathology, Volume II by : Estela Maris Muñoz

Download or read book Transcription Regulation - Brain Development and Homeostasis - A Finely Tuned and Orchestrated Scenario in Physiology and Pathology, Volume II written by Estela Maris Muñoz and published by Frontiers Media SA. This book was released on 2023-10-03 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: A finely tuned regulation of gene expression is essential for shaping the nervous system and for maintaining its homeostasis throughout life. Disruptions in gene regulation can impact brain development and physiology in ways that contribute to diverse pathologies. The master orchestrators of gene activity in the nucleus are transcription factors, proteins that recognize and bind to specific DNA motifs in regulatory regions and drive changes in gene expression. Transcription factors act with the help of other co-factor proteins, including components of the Mediator complex, histone modifying enzymes, chromatin modelers, and DNA methylases. In addition, transcription factor activity in the nervous system can be modulated by extracellular signals, including growth factors, hormones, neuropeptides and neurotransmitters that activate specific receptors and intracellular transduction pathways. An in-depth understanding of the mechanisms of transcription regulation is needed in order to better describe how each element, from genes to cells, defines and maintains identities and functionalities in the healthy and diseased brain. This Research Topic is oriented to developing an integrative view about transcription regulation within the nervous system, focusing on developmental and homeostatic processes, dysregulation in functionality and expression levels and consequent associated pathologies such as neurodevelopmental disorders, brain tumors, and neurodegenerative diseases. Transcription regulation investigations will specifically focus on transcription factors that belong to the bHLH (e.g. NeuroD), homeobox (e.g. Islet, Pax, Rax, and Lhx) and CREB families, and on their roles over defined nervous system areas: cerebral cortex, thalamic and hypothalamic areas, interacting with the developing brain.

New Serial Titles

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Publisher :
ISBN 13 :
Total Pages : 1776 pages
Book Rating : 4.:/5 (31 download)

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Book Synopsis New Serial Titles by :

Download or read book New Serial Titles written by and published by . This book was released on 1990 with total page 1776 pages. Available in PDF, EPUB and Kindle. Book excerpt: A union list of serials commencing publication after Dec. 31, 1949.

Molecular Chaperones—Advances in Research and Application: 2013 Edition

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Author :
Publisher : ScholarlyEditions
ISBN 13 : 1481689932
Total Pages : 224 pages
Book Rating : 4.4/5 (816 download)

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Book Synopsis Molecular Chaperones—Advances in Research and Application: 2013 Edition by :

Download or read book Molecular Chaperones—Advances in Research and Application: 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Chaperones—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Histone Chaperones. The editors have built Molecular Chaperones—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Histone Chaperones in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Molecular Chaperones—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Microbiologia

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Publisher :
ISBN 13 :
Total Pages : 588 pages
Book Rating : 4.:/5 ( download)

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Book Synopsis Microbiologia by :

Download or read book Microbiologia written by and published by . This book was released on 1997 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Aquaporins: Dynamic Role and Regulation

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Publisher : Frontiers Media SA
ISBN 13 : 2889452891
Total Pages : 185 pages
Book Rating : 4.8/5 (894 download)

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Book Synopsis Aquaporins: Dynamic Role and Regulation by : Rupesh K. Deshmukh

Download or read book Aquaporins: Dynamic Role and Regulation written by Rupesh K. Deshmukh and published by Frontiers Media SA. This book was released on 2017-10-10 with total page 185 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aquaporins (AQPs), a class of integral membrane proteins, form channels facilitating movement of water and many other solutes. In solute transport systems of all living organisms including plants, animals and fungi, AQPs play a vital role. Plants contain a much higher number of AQP genes compared to animals, the likely consequence of genome duplication events and higher ploidy levels. As a result of duplication and subsequent diversification, plant AQPs have evolved several subfamilies with very diverse functions. Plant AQPs are highly selective for specific solutes because of their unique structural features. For instance, ar/R selectivity filters and NPA domains have been found to be key elements in governing solute permeability through the AQP channels. Combination of conserved motifs and specific amino acids influencing pore morphology appears to regulate the permeability of specific solutes such as water, urea, CO2, H2O2, boric acid, silicic acid and many more. The discovery of novel AQPs has been accelerated over the last few years with the increasing availability of genomic and transcriptomic data. The expanding number of well characterised AQPs provides opportunities to understand factors influencing water transport, nutritional uptake, and elemental balance. Homology-based search tools and phylogenetic analyses offer efficient strategies for AQP identification. Subsequent characterization can be based on different approaches involving proteomics, genomics, and transcriptomic tools. The combination of these technological advances make it possible to efficiently study the inter-dependency of AQPs, regulation through phosphorylation and reversible phosphorylation, networking with other transporters, structural features, pH gating systems, trafficking and degradation. Several studies have supported the role of AQPs in differential phenotypic responses to abiotic and biotic stress in plants. Crop improvement programs aiming for the development of cultivars with higher tolerance against stresses like drought, flooding, salinity and many biotic diseases, can explore and exploit the finely tuned AQP-regulated transport system. For instance, a promising approach in crop breeding programs is the utilization of genetic variation in AQPs for the development of stress tolerant cultivars. Similarly, transgenic and mutagenesis approaches provide an opportunity to better understand the AQP transport system with subsequent applications for the development of climate-smart drought-tolerant cultivars. The contributions to this Frontiers in Plant Science Research Topic have highlighted the evolution and phylogenetic distribution of AQPs in several plant species. Numerous aspects of regulation that seek to explain AQP-mediated transport system have been addressed. These contributions will help to improve our understanding of AQPs and their role in important physiological aspects and will bring AQP research closer to practical applications.