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Author : Andrew W. Rogers
Publisher :
ISBN 13 : 9780444410573
Total Pages : 372 pages
Book Rating : 4.4/5 (15 download)
Download or read book Techniques of Autoradiography written by Andrew W. Rogers and published by . This book was released on 1973 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Renato Baserga
Publisher :
ISBN 13 :
Total Pages : 304 pages
Book Rating : 4.3/5 (91 download)
Download or read book Autoradiography: Techniques and Application written by Renato Baserga and published by . This book was released on 1969 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : George A. Boyd
Publisher : Academic Press
ISBN 13 : 1483282333
Total Pages : 416 pages
Book Rating : 4.4/5 (832 download)
Download or read book Autoradiography in Biology and Medicine written by George A. Boyd and published by Academic Press. This book was released on 2013-10-22 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autoradiography in Biology and Medicine focuses on the applications of autoradiography in medicine and biology, including photographic processes, emulsions, and response of films to isotopes and x-rays. The book first offers information on the meaning of autoradiography, including the etymology of autoradiography, photographic emulsion as a scientific instrument, and the relationship of biologists and autoradiography. The publication then examines the photographic process and comparative response of commercial and scientific emulsions. Discussions focus on fixation, washing, emulsion, response of films to electrons, x-rays, and gamma rays, and response of films to beta ray spectra of isotopes. The text takes a look at commercial photographic materials for autoradiography, autoradiographic image, and resolution. The manuscript then reviews the estimation of dose and exposure time, sources of error, and techniques. Topics include chemical variables, estimation of exposure time, errors occurring during the preparation of the autoradiogram for exposure, and contour autoradiography. The book is a valuable reference for readers interested in autoradiography.
Author : C. G. Curtis
Publisher :
ISBN 13 :
Total Pages : 160 pages
Book Rating : 4.F/5 ( download)
Download or read book Whole-body Autoradiography written by C. G. Curtis and published by . This book was released on 1981 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Brigitte Schultze
Publisher : Academic Press
ISBN 13 : 1483194876
Total Pages : 313 pages
Book Rating : 4.4/5 (831 download)
Download or read book Autoradiography at the Cellular Level written by Brigitte Schultze and published by Academic Press. This book was released on 2013-10-22 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium. This book reviews the different autoradiographic techniques and difficulties in theoretical principles needed for the interpretation of autoradiograms. The topics include the characteristics of the different types of radiation; autoradiographic resolution; techniques for applying photographic emulsions; and autoradiography of water-soluble substances. The application of different isotopes; general aspects of the use of thymidine; physiological RNA synthesis; and RNA synthesis under pathological conditions are also elaborated. This text likewise covers the protein synthesis under normal conditions; electron microscopic autoradiography; application of electron microscopic autoradiography; and autoradiographic methods in cytochemistry. This publication is a good reference for students, researchers, and clinicians conducting work on the use of tritiated compounds in high resolution autoradiography.
Author : P Gahan
Publisher : Elsevier
ISBN 13 : 0323161057
Total Pages : 137 pages
Book Rating : 4.3/5 (231 download)
Download or read book Autoradiography for Biologists written by P Gahan and published by Elsevier. This book was released on 2012-12-02 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autoradiography for Biologists discusses the basic and practical aspects of various autoradiography techniques. It is composed of six chapters; each chapter covers a specific form of autoradiography technique. Chapter 1 discusses various theoretical aspects of autoradiography, such as radioactive decay, beta-particle absorption, photographic process, sensitivity, and resolving power. Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro-, stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography. Practical and theoretical details of the technique, together with modifications made, are covered in each chapter. This book will be both an encouragement and a help to people wanting to apply autoradiography to the solution of biological problems.
Author : Arun Sharma
Publisher : Butterworth-Heinemann
ISBN 13 : 1483100847
Total Pages : 724 pages
Book Rating : 4.4/5 (831 download)
Download or read book Chromosome Techniques written by Arun Sharma and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.
Author : Andrew W. Rogers
Publisher :
ISBN 13 :
Total Pages : 360 pages
Book Rating : 4.3/5 (91 download)
Download or read book Techniques of Autoradiography written by Andrew W. Rogers and published by . This book was released on 1969 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : H. A. Fischer
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3112318498
Total Pages : 212 pages
Book Rating : 4.1/5 (123 download)
Download or read book Autoradiography written by H. A. Fischer and published by Walter de Gruyter GmbH & Co KG. This book was released on 2020-05-18 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: No detailed description available for "Autoradiography".
Author : E. Passarge
Publisher : Springer Science & Business Media
ISBN 13 : 3642657877
Total Pages : 389 pages
Book Rating : 4.6/5 (426 download)
Download or read book Methods in Human Cytogenetics written by E. Passarge and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume was originally intended to be an English translation of the book MetllOden in der medizinischen Cytogenetik, published in 1970. Just about then, however, a number of new techniques were introduced in human cytogenetics and soon acquired the utmost importance, parti cularly in clinical diagnosis, so that the English edition had to be con siderably enlarged. As a result, there are now twelve chapters instead of eight, and two additional authors have been called upon, Dr. KRONE and Dr. SCHNEDL. In addition to the up-to-date presentation of con ventional methods of cell culture and techniques for the preparation and identification of human chromosomes, this text covers the various tech niques of producing banding patterns and applying them in chromo some identification. Further, it deals with the culture of amniotic fluid cells and gives instructions for handling tissue-culture cells for bio chemical analysis; it thus meets the ever-increasing requirements of a modern cell-culture laboratory. To paraphrase the aims of this book, we quote part of the preface to the German edition: "It was intended to collect the various methods so as to make them accessible for laboratory use. Furthermore, it is hoped that the reader faced with current research problems will be stimulated to modify and supplement the techniques described, instead of merely applying them automatically. In a rapidly developing field, some methods are still preliminary, and no final presentation seems possible.
Author : George A. Boyd
Publisher :
ISBN 13 :
Total Pages : 12 pages
Book Rating : 4.3/5 (91 download)
Download or read book Stripping Film Techniques for Histological Autoradiographs written by George A. Boyd and published by . This book was released on 1949 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Bonnie S. Dunbar
Publisher : Springer
ISBN 13 : 9780306428395
Total Pages : 398 pages
Book Rating : 4.4/5 (283 download)
Download or read book Two-Dimensional Electrophoresis and Immunological Techniques written by Bonnie S. Dunbar and published by Springer. This book was released on 1988-07-31 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is a summary of basic principles and techniques and is dedicated to all those students who have been told by their mentors, "Go forth and do two-dimensional gels and have the results on my desk tomorrow. " No attempt has been made in this text to provide exhaustive lists of references related to basic principles or techniques or to list every com pany or supplier involved in this area of research. Nevertheless, it is hoped that sufficient information is given to help a new investigator or student appreciate the complexities but develop sufficient expertise to carry out these techniques successfully. The discussions are designed to instill in basic science and clinical investigators of all levels of expertise an appreciation of the power of combining a variety of techniques as well as to provide basic insight into the theories, complexities, and problems frequently encountered with electrophoretic and immunochemical meth ods. Bonnie S. Dunbar Houston v Acknowledgments I wish to thank my students and staff for their patience and support throughout the preparation of this text. I would like to acknowledge my appreciation for my extensive discussions with Dr. David Sammons (Uni versity of Arizona) and to Dr. N. L. and Dr. N. G. Anderson and their colleagues (Argonne National Laboratory) for their invaluable advice and suggestions in this area over the years. I thank my research assistant, Ms.
Author : V. Grouls
Publisher : Springer Science & Business Media
ISBN 13 : 3642685145
Total Pages : 79 pages
Book Rating : 4.6/5 (426 download)
Download or read book The Development of the Red Pulp in the Spleen written by V. Grouls and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 79 pages. Available in PDF, EPUB and Kindle. Book excerpt: In many aspects hematopoiesis in newborn rodents, especially in rats, resembles hema topoiesis in the human fetus in the 6th-7th month of gestation. In man the transition from the stage of liver to bone marrow erythropoiesis takes place at this time (Bessis, 1973). In rodents, however, the liver is almost the only place where hematopoiesis occurs until birth. Thereafter it is replaced to a growing extent by the bone marrow, which so far consists mainly of immature mesenchymal cells (Maximow, 1910; Cuda, 1970). Thus hematopoietic precursor cells appear in the sternum only around 30 h after birth. Just as in premature human infants, a macrocytic anemia can be demonstrat ed in normal neonatal rats (Lucarelli et aI., 1964, 1968). Beside liver (fetal) and bone marrow, the spleen is involved in hematopoiesis. In rodents like rats and mice, splenic hematopoiesis persists more or less markedly until adulthood; in man, however, it ceases after birth and reappears only under certain pathological conditions (Fischer et aI., 1970; Hennekeuser et aI., 1967; Fresen, 1960).
Author : J.K. Koehler
Publisher : Springer Science & Business Media
ISBN 13 : 3642654924
Total Pages : 319 pages
Book Rating : 4.6/5 (426 download)
Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Author : M.A. Williams
Publisher : Elsevier
ISBN 13 : 1483293874
Total Pages : 238 pages
Book Rating : 4.4/5 (832 download)
Download or read book Autoradiography and Immunocytochemistry written by M.A. Williams and published by Elsevier. This book was released on 2013-10-22 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
Author : John R. Baker
Publisher : Garland Science
ISBN 13 : 9780198564225
Total Pages : 110 pages
Book Rating : 4.5/5 (642 download)
Download or read book Autoradiography written by John R. Baker and published by Garland Science. This book was released on 1989 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: Of interest to both students and biological scientists in a wide variety of research areas, this handbook provides a comprehensive overview of the major techniques of autoradiography. The reader will find detailed, practical instructions, as well as information on the rationale behind the methods, and a critical appraisal of autoradiographs. With this volume the author, a widely known expert in the field, provides an invaluable introduction to the theory and techniques of autoradiography and an important addition to the library of experienced researchers who are familiar with other methods, but would like to broaden the scope of their knowledge. The volume includes numerous illustrations and helpful lists of further readings.
Author : R.G. Hassler
Publisher : Springer
ISBN 13 : 1489965270
Total Pages : 472 pages
Book Rating : 4.4/5 (899 download)
Download or read book Evolution of the Forebrain written by R.G. Hassler and published by Springer. This book was released on 2013-12-11 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
